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- EMDB-3985: PilQ from Thermus thermophilus -

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Basic information

Entry
Database: EMDB / ID: 3985
TitlePilQ from Thermus thermophilus
Map data
SamplePilQ complex with 13-fold symmetry:
PilQ
SourceThermus thermophilus HB27 (bacteria)
Methodsingle particle reconstruction / cryo EM / 20.4 Å resolution
AuthorsD'Imprima E / Vonck J / Sanchez R
CitationJournal: Elife / Year: 2017
Title: Cryo-EM structure of the bifunctional secretin complex of .
Authors: Edoardo D'Imprima / Ralf Salzer / Ramachandra M Bhaskara / Ricardo Sánchez / Ilona Rose / Lennart Kirchner / Gerhard Hummer / Werner Kühlbrandt / Janet Vonck / Beate Averhoff
DateDeposition: Nov 15, 2017 / Header (metadata) release: Nov 29, 2017 / Map release: Jan 10, 2018 / Last update: Mar 28, 2018

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.025
  • Imaged by UCSF Chimera
  • Download
  • Surface view colored by cylindrical radius
  • Surface level: 0.025
  • Imaged by UCSF Chimera
  • Download
Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

Downloads & links

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Map

Fileemd_3985.map.gz (map file in CCP4 format, 136049 KB)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
324 pix
1.63 Å/pix.
= 528.12 Å
324 pix
1.63 Å/pix.
= 528.12 Å
324 pix
1.63 Å/pix.
= 528.12 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.63 Å
Density
Contour Level:0.025 (by author), 0.025 (movie #1):
Minimum - Maximum-0.017303573 - 0.05273688
Average (Standard dev.)0.00046973448 (0.0040036784)
Details

EMDB XML:

Space Group Number1
Map Geometry
Axis orderXYZ
Dimensions324324324
Origin000
Limit323323323
Spacing324324324
CellA=B=C: 528.12 Å
α=β=γ: 90.0 deg.

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.631.631.63
M x/y/z324324324
origin x/y/z0.0000.0000.000
length x/y/z528.120528.120528.120
α/β/γ90.00090.00090.000
start NX/NY/NZ
NX/NY/NZ
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS324324324
D min/max/mean-0.0170.0530.000

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Supplemental data

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Sample components

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Entire PilQ complex with 13-fold symmetry

EntireName: PilQ complex with 13-fold symmetry / Number of components: 2
MassTheoretical: 1000 kDa

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Component #1: protein, PilQ complex with 13-fold symmetry

ProteinName: PilQ complex with 13-fold symmetry / Recombinant expression: No
MassTheoretical: 1000 kDa
SourceSpecies: Thermus thermophilus HB27 (bacteria)

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Component #2: protein, PilQ

ProteinName: PilQ / Recombinant expression: No
SourceSpecies: Thermus thermophilus HB27 (bacteria)

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Experimental details

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Sample preparation

SpecimenSpecimen state: particle / Method: cryo EM
Sample solutionpH: 8.5
VitrificationInstrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE / Temperature: 283 K / Humidity: 7 % / Details: blotting for 8-10 seconds

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Electron microscopy imaging

ImagingMicroscope: JEOL 3200FSC
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Electron dose: 33 e/Å2 / Illumination mode: FLOOD BEAM
LensMagnification: 20000 X (nominal), 30675 X (calibrated) / Cs: 4.2 mm / Imaging mode: BRIGHT FIELD / Energy filter: In-column Omega Filter / Energy window: 18-18 eV
Specimen HolderModel: JEOL
CameraDetector: GATAN K2 (4k x 4k)

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Image processing

ProcessingMethod: single particle reconstruction / Applied symmetry: C13 (13 fold cyclic) / Number of projections: 2646
3D reconstructionSoftware: RELION / Resolution: 20.4 Å / Resolution method: FSC 0.143 CUT-OFF

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