+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-39102 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Title | ATAT-2 bound K40Q MEC-12/MEC-7 microtubule | |||||||||
Map data | Major map | |||||||||
Sample |
| |||||||||
Keywords | Microtubule / luminal enzyme / PTM / STRUCTURAL PROTEIN | |||||||||
Function / homology | Function and homology information positive regulation of detection of mechanical stimulus involved in sensory perception of touch / positive regulation of mechanosensory behavior / COPI-mediated anterograde transport / COPI-independent Golgi-to-ER retrograde traffic / COPI-dependent Golgi-to-ER retrograde traffic / Kinesins / Intraflagellar transport / alpha-tubulin N-acetyltransferase / tubulin N-acetyltransferase activity / detection of mechanical stimulus involved in sensory perception of touch ...positive regulation of detection of mechanical stimulus involved in sensory perception of touch / positive regulation of mechanosensory behavior / COPI-mediated anterograde transport / COPI-independent Golgi-to-ER retrograde traffic / COPI-dependent Golgi-to-ER retrograde traffic / Kinesins / Intraflagellar transport / alpha-tubulin N-acetyltransferase / tubulin N-acetyltransferase activity / detection of mechanical stimulus involved in sensory perception of touch / thigmotaxis / detection of mechanical stimulus involved in sensory perception / mechanosensory behavior / lysine N-acetyltransferase activity, acting on acetyl phosphate as donor / microtubule-based process / neuron development / regulation of microtubule cytoskeleton organization / cytoplasmic microtubule organization / response to mechanical stimulus / structural constituent of cytoskeleton / microtubule cytoskeleton organization / mitotic cell cycle / Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement / microtubule / hydrolase activity / neuron projection / axon / GTPase activity / neuronal cell body / GTP binding / metal ion binding / cytoplasm Similarity search - Function | |||||||||
Biological species | Caenorhabditis elegans (invertebrata) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.1 Å | |||||||||
Authors | Lam WH / Yu D / Zhai Y / Ti S | |||||||||
Funding support | 1 items
| |||||||||
Citation | Journal: Nat Struct Mol Biol / Year: 2024 Title: Tubulin acetyltransferases access and modify the microtubule luminal K40 residue through anchors in taxane-binding pockets. Authors: Jingyi Luo / Wai Hei Lam / Daqi Yu / Victor C Chao / Marc Nicholas Zopfi / Chen Jing Khoo / Chang Zhao / Shan Yan / Zheng Liu / Xiang David Li / Chaogu Zheng / Yuanliang Zhai / Shih-Chieh Ti / Abstract: Acetylation at α-tubulin K40 is the sole post-translational modification preferred to occur inside the lumen of hollow cylindrical microtubules. However, how tubulin acetyltransferases access the ...Acetylation at α-tubulin K40 is the sole post-translational modification preferred to occur inside the lumen of hollow cylindrical microtubules. However, how tubulin acetyltransferases access the luminal K40 in micrometer-long microtubules remains unknown. Here, we use cryo-electron microscopy and single-molecule reconstitution assays to reveal the enzymatic mechanism for tubulin acetyltransferases to modify K40 in the lumen. One tubulin acetyltransferase spans across the luminal lattice, with the catalytic core docking onto two α-tubulins and the enzyme's C-terminal domain occupying the taxane-binding pockets of two β-tubulins. The luminal accessibility and enzyme processivity of tubulin acetyltransferases are inhibited by paclitaxel, a microtubule-stabilizing chemotherapeutic agent. Characterizations using recombinant tubulins mimicking preacetylated and postacetylated K40 show the crosstalk between microtubule acetylation states and the cofactor acetyl-CoA in enzyme turnover. Our findings provide crucial insights into the conserved multivalent interactions involving α- and β-tubulins to acetylate the confined microtubule lumen. | |||||||||
History |
|
-Structure visualization
Supplemental images |
---|
-Downloads & links
-EMDB archive
Map data | emd_39102.map.gz | 121.8 MB | EMDB map data format | |
---|---|---|---|---|
Header (meta data) | emd-39102-v30.xml emd-39102.xml | 23.6 KB 23.6 KB | Display Display | EMDB header |
FSC (resolution estimation) | emd_39102_fsc.xml | 13.1 KB | Display | FSC data file |
Images | emd_39102.png | 44 KB | ||
Filedesc metadata | emd-39102.cif.gz | 7 KB | ||
Others | emd_39102_additional_1.map.gz emd_39102_half_map_1.map.gz emd_39102_half_map_2.map.gz | 230.1 MB 226.3 MB 226.3 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-39102 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-39102 | HTTPS FTP |
-Validation report
Summary document | emd_39102_validation.pdf.gz | 964.6 KB | Display | EMDB validaton report |
---|---|---|---|---|
Full document | emd_39102_full_validation.pdf.gz | 964.1 KB | Display | |
Data in XML | emd_39102_validation.xml.gz | 22.3 KB | Display | |
Data in CIF | emd_39102_validation.cif.gz | 29 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-39102 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-39102 | HTTPS FTP |
-Related structure data
Related structure data | 8yalMC 8y9fC 8yajC 8yarC M: atomic model generated by this map C: citing same article (ref.) |
---|---|
Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
---|---|
Related items in Molecule of the Month |
-Map
File | Download / File: emd_39102.map.gz / Format: CCP4 / Size: 244.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Annotation | Major map | ||||||||||||||||||||||||||||||||||||
Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.06 Å | ||||||||||||||||||||||||||||||||||||
Density |
| ||||||||||||||||||||||||||||||||||||
Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
|
-Supplemental data
-Additional map: Sharpened map
File | emd_39102_additional_1.map | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Annotation | Sharpened map | ||||||||||||
Projections & Slices |
| ||||||||||||
Density Histograms |
-Half map: #2
File | emd_39102_half_map_1.map | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Projections & Slices |
| ||||||||||||
Density Histograms |
-Half map: #1
File | emd_39102_half_map_2.map | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Projections & Slices |
| ||||||||||||
Density Histograms |
-Sample components
-Entire : K40Q MEC-12/MEC-7 microtubule complexed with alpha TAT2
Entire | Name: K40Q MEC-12/MEC-7 microtubule complexed with alpha TAT2 |
---|---|
Components |
|
-Supramolecule #1: K40Q MEC-12/MEC-7 microtubule complexed with alpha TAT2
Supramolecule | Name: K40Q MEC-12/MEC-7 microtubule complexed with alpha TAT2 type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#3 |
---|---|
Source (natural) | Organism: Caenorhabditis elegans (invertebrata) |
-Macromolecule #1: Tubulin alpha-3 chain
Macromolecule | Name: Tubulin alpha-3 chain / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO EC number: Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement |
---|---|
Source (natural) | Organism: Caenorhabditis elegans (invertebrata) |
Molecular weight | Theoretical: 50.165367 KDa |
Recombinant expression | Organism: Trichoplusia ni (cabbage looper) |
Sequence | String: MREVISIHIG QAGVQIGNAC WELYCLEHGI QPDGQMPSDQ SLGGSDDSFS TFFSETGSGR HVPRAVMVDL EPTVIDEIRT GTYRSLFHP EQLITGKEDA ANNYARGHYT IGKEIIDLTL DRIRRLADNC TGLQGFLVFH SFGGGTGSGF TSLLMERLSV D YGKKAKLE ...String: MREVISIHIG QAGVQIGNAC WELYCLEHGI QPDGQMPSDQ SLGGSDDSFS TFFSETGSGR HVPRAVMVDL EPTVIDEIRT GTYRSLFHP EQLITGKEDA ANNYARGHYT IGKEIIDLTL DRIRRLADNC TGLQGFLVFH SFGGGTGSGF TSLLMERLSV D YGKKAKLE FSIYPAPQVS TAVVEPYNSI LTTHTTLEHS DCSFMVDNEA IYDICRRNLD IERPSYTNLN RLIGQIVSSI TA SLRFDGA LNVDLTEFQT NLVPYPRIHF PLATFSPVIS AEKAYHEQLS VAEITNMCFE PHNQMVKCDP RHGKYMAVCL LFR GDVVPK DVNAAIATIK TKRSIQFVDW CPTGFKVGIN YQPPTVVPGG DLAKVPRAVC MLSNTTAIAE AWARLDHKFD LMYA KRAFV HWYVGEGMEE GEFSEAREDL AALEKDYEEV GVDSMEDNGE EGDEY UniProtKB: Tubulin alpha-3 chain |
-Macromolecule #2: Alpha-tubulin N-acetyltransferase 2
Macromolecule | Name: Alpha-tubulin N-acetyltransferase 2 / type: protein_or_peptide / ID: 2 / Number of copies: 2 / Enantiomer: LEVO / EC number: alpha-tubulin N-acetyltransferase |
---|---|
Source (natural) | Organism: Caenorhabditis elegans (invertebrata) |
Molecular weight | Theoretical: 30.434654 KDa |
Recombinant expression | Organism: Trichoplusia ni (cabbage looper) |
Sequence | String: MEIAFDLSTI FTDNIQRLTR TDLLKYGPKR YWAVAQSIDC LGEMSSKFHG WKRVITMYDK IVDHDEEQTT YIMWEKVNGS KSILKGLLR VGYKTLYLTD NEQNQYMEKA MCILDFFVVP TEQRSGNGFK MFDEMLKAEN VTVDQCAFDK PSAALQQFLE K YYDRKDLV ...String: MEIAFDLSTI FTDNIQRLTR TDLLKYGPKR YWAVAQSIDC LGEMSSKFHG WKRVITMYDK IVDHDEEQTT YIMWEKVNGS KSILKGLLR VGYKTLYLTD NEQNQYMEKA MCILDFFVVP TEQRSGNGFK MFDEMLKAEN VTVDQCAFDK PSAALQQFLE K YYDRKDLV WQSNKYALCS NFFIGRHPTV PFTPRQTKRA SRASSAVSSH ASSRNTSPIG RNRPRHDSVA DLMRQDMLAG VR AEVDPNS PTGLKNARDF GHRRIW UniProtKB: Alpha-tubulin N-acetyltransferase 2 |
-Macromolecule #3: Tubulin beta-1 chain
Macromolecule | Name: Tubulin beta-1 chain / type: protein_or_peptide / ID: 3 / Details: MEC-7 / Number of copies: 2 / Enantiomer: LEVO |
---|---|
Source (natural) | Organism: Caenorhabditis elegans (invertebrata) |
Molecular weight | Theoretical: 49.306176 KDa |
Recombinant expression | Organism: Trichoplusia ni (cabbage looper) |
Sequence | String: MREIVHIQAG QCGNQIGSKF WEVISDEHGI DPSGQYVGDS DLQLERINVY YNEAGSNKYV PRAVLVDLEP GTMDSVRSGP FGQLFRPDN YVFGQSGAGN NWAKGHYTEG AELVDNVLDV VRKEAESTDC LQGFQLTHSL GGGTGSGMGT LLISKIREEY P DRIMNTFS ...String: MREIVHIQAG QCGNQIGSKF WEVISDEHGI DPSGQYVGDS DLQLERINVY YNEAGSNKYV PRAVLVDLEP GTMDSVRSGP FGQLFRPDN YVFGQSGAGN NWAKGHYTEG AELVDNVLDV VRKEAESTDC LQGFQLTHSL GGGTGSGMGT LLISKIREEY P DRIMNTFS VVPSPKVSDT VVEPYNATLS VHQLVENTDS TFCIDNEALY DICFRTLKLT TPTYGDLNHL VSATMSGVTT CL RFPGQLN ADLRKLAVNM VPFPRLHFFM PGFAPLTSRS NQQYRAITVP ELTQQCFDAK NMMAACDPRH GRYLTAAAIF RGR MSMKEV DEQMLNIQNK NSSYFVDWIP NNVKTAVCDI PPRGLKMSAT FIGNSTAIQE LFKRISEQFT AMFRRKAFLH WYTG EGMDE MEFTEAESNM NDLVSEYQQY QEAAADEDAA EAFDGE UniProtKB: Tubulin beta-1 chain |
-Macromolecule #4: GUANOSINE-5'-TRIPHOSPHATE
Macromolecule | Name: GUANOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 4 / Number of copies: 2 / Formula: GTP |
---|---|
Molecular weight | Theoretical: 523.18 Da |
Chemical component information | ChemComp-GTP: |
-Macromolecule #5: ACETYL COENZYME *A
Macromolecule | Name: ACETYL COENZYME *A / type: ligand / ID: 5 / Number of copies: 1 / Formula: ACO |
---|---|
Molecular weight | Theoretical: 809.571 Da |
Chemical component information | ChemComp-ACO: |
-Macromolecule #6: PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER
Macromolecule | Name: PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER / type: ligand / ID: 6 / Number of copies: 2 / Formula: G2P |
---|---|
Molecular weight | Theoretical: 521.208 Da |
Chemical component information | ChemComp-G2P: |
-Experimental details
-Structure determination
Method | cryo EM |
---|---|
Processing | single particle reconstruction |
Aggregation state | helical array |
-Sample preparation
Buffer | pH: 6.8 Component:
| ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Grid | Model: C-flat-1.2/1.3 / Material: GOLD / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 45 sec. | ||||||||||||
Vitrification | Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 298 K / Instrument: FEI VITROBOT MARK IV |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
---|---|
Image recording | Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 50.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | C2 aperture diameter: 100.0 µm / Calibrated defocus max: 2.2 µm / Calibrated defocus min: 1.1 µm / Calibrated magnification: 47170 / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 2.2 µm / Nominal defocus min: 1.1 µm / Nominal magnification: 81000 |
Sample stage | Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
+Image processing
-Atomic model buiding 1
Refinement | Protocol: RIGID BODY FIT |
---|---|
Output model | PDB-8yal: |