Japan Agency for Medical Research and Development (AMED)
JP23fm0208101
日本
Japan Agency for Medical Research and Development (AMED)
JP22fm0208101
日本
Japan Agency for Medical Research and Development (AMED)
22wm0325023h9903
日本
Japan Agency for Medical Research and Development (AMED)
JP23fm0208101
日本
Japan Agency for Medical Research and Development (AMED)
22fk0108552h0001
日本
Japan Agency for Medical Research and Development (AMED)
JP23ama121001
日本
引用
ジャーナル: Nat Commun / 年: 2025 タイトル: Structural basis for Ebola virus nucleocapsid assembly and function regulated by VP24. 著者: Yoko Fujita-Fujiharu / Shangfan Hu / Ai Hirabayashi / Yuki Takamatsu / Yen Ni Ng / Kazuya Houri / Yukiko Muramoto / Masahiro Nakano / Yukihiko Sugita / Takeshi Noda / 要旨: The Ebola virus, a member of the Filoviridae family, causes severe hemorrhagic fever in humans. Filamentous virions contain a helical nucleocapsid responsible for genome transcription, replication, ...The Ebola virus, a member of the Filoviridae family, causes severe hemorrhagic fever in humans. Filamentous virions contain a helical nucleocapsid responsible for genome transcription, replication, and packaging into progeny virions. The nucleocapsid consists of a helical nucleoprotein (NP)-viral genomic RNA complex forming the core structure, to which VP24 and VP35 bind externally. Two NPs, each paired with a VP24 molecule, constitute a repeating unit. However, the detailed nucleocapsid structure remains unclear. Here, we determine the nucleocapsid-like structure within virus-like particles at 4.6 Å resolution using single-particle cryo-electron microscopy. Mutational analysis identifies specific interactions between the two NPs and two VP24s and demonstrates that each of the two VP24s in different orientations distinctively regulates nucleocapsid assembly, viral RNA synthesis, intracellular transport of the nucleocapsid, and infectious virion production. Our findings highlight the sophisticated mechanisms underlying the assembly and functional regulation of the nucleocapsid and provide insights into antiviral development.
凍結剤: ETHANE / チャンバー内湿度: 100 % / チャンバー内温度: 281.15 K / 装置: FEI VITROBOT MARK IV 詳細: The sample was applied to both sides of the grid. The grids were blotted for 14 seconds..
詳細
The total 2.5 microlitre sample was applied to both sides of the glow-discharged grid.
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電子顕微鏡法
顕微鏡
TFS KRIOS
特殊光学系
球面収差補正装置: Microscope was modified with a Cs corrector (CEOS GmbH) エネルギーフィルター - 名称: GIF Bioquantum / エネルギーフィルター - スリット幅: 20 eV