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TitleStructural basis for Ebola virus nucleocapsid assembly and function regulated by VP24.
Journal, issue, pagesNat Commun, Vol. 16, Issue 1, Page 2171, Year 2025
Publish dateMar 10, 2025
AuthorsYoko Fujita-Fujiharu / Shangfan Hu / Ai Hirabayashi / Yuki Takamatsu / Yen Ni Ng / Kazuya Houri / Yukiko Muramoto / Masahiro Nakano / Yukihiko Sugita / Takeshi Noda /
PubMed AbstractThe Ebola virus, a member of the Filoviridae family, causes severe hemorrhagic fever in humans. Filamentous virions contain a helical nucleocapsid responsible for genome transcription, replication, ...The Ebola virus, a member of the Filoviridae family, causes severe hemorrhagic fever in humans. Filamentous virions contain a helical nucleocapsid responsible for genome transcription, replication, and packaging into progeny virions. The nucleocapsid consists of a helical nucleoprotein (NP)-viral genomic RNA complex forming the core structure, to which VP24 and VP35 bind externally. Two NPs, each paired with a VP24 molecule, constitute a repeating unit. However, the detailed nucleocapsid structure remains unclear. Here, we determine the nucleocapsid-like structure within virus-like particles at 4.6 Å resolution using single-particle cryo-electron microscopy. Mutational analysis identifies specific interactions between the two NPs and two VP24s and demonstrates that each of the two VP24s in different orientations distinctively regulates nucleocapsid assembly, viral RNA synthesis, intracellular transport of the nucleocapsid, and infectious virion production. Our findings highlight the sophisticated mechanisms underlying the assembly and functional regulation of the nucleocapsid and provide insights into antiviral development.
External linksNat Commun / PubMed:40064872 / PubMed Central
MethodsEM (single particle)
Resolution4.6 Å
Structure data

39081

8y9j

EMDB-39081, PDB-8y9j:
Structure of the Ebola virus nucleocapsid subunit
Method: EM (single particle) / Resolution: 4.6 Å

Source
  • zaire ebolavirus
  • homo sapiens (human)
KeywordsVIRAL PROTEIN/RNA / Complex / VIRAL PROTEIN / VIRAL PROTEIN-RNA complex

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