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- EMDB-38993: Cryo-EM Structure of the human minor pre-B complex (pre-precataly... -

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Basic information

Entry
Database: EMDB / ID: EMD-38993
TitleCryo-EM Structure of the human minor pre-B complex (pre-precatalytic spliceosome) U11 and tri-snRNP part
Map data
Sample
  • Complex: human minor pre-B complex
    • RNA: x 5 types
    • Protein or peptide: x 27 types
  • Ligand: x 4 types
Keywordsspliceosome / minor spliceosome / U11 snRNP / U12 snRNP / U4atac / U6atac / CENATAC / TXNL4B / U11 snRNA / minor tri-snRNP / U12-type intron / SPLICING/RNA / SPLICING-RNA complex
Function / homology
Function and homology information


negative regulation of centrosome duplication / spliceosomal snRNP complex / ribonucleoprotein complex localization / U4atac snRNP / positive regulation of cytotoxic T cell differentiation / maturation of 5S rRNA / RNA localization / U4/U6 snRNP / U4atac snRNA binding / chromosome separation ...negative regulation of centrosome duplication / spliceosomal snRNP complex / ribonucleoprotein complex localization / U4atac snRNP / positive regulation of cytotoxic T cell differentiation / maturation of 5S rRNA / RNA localization / U4/U6 snRNP / U4atac snRNA binding / chromosome separation / R-loop processing / regulation of mitotic cell cycle spindle assembly checkpoint / box C/D sno(s)RNA binding / positive regulation of hippo signaling / snRNA binding / U2 snRNP binding / rRNA 2'-O-methylation / dense fibrillar component / U7 snRNA binding / histone pre-mRNA DCP binding / U7 snRNP / cis assembly of pre-catalytic spliceosome / histone pre-mRNA 3'end processing complex / nuclear histone mRNA catabolic process / SLBP independent Processing of Histone Pre-mRNAs / SLBP Dependent Processing of Replication-Dependent Histone Pre-mRNAs / box C/D methylation guide snoRNP complex / 7-methylguanosine cap hypermethylation / U12-type spliceosomal complex / U1 snRNP binding / pICln-Sm protein complex / U2-type catalytic step 1 spliceosome / methylosome / RNA splicing, via transesterification reactions / sno(s)RNA-containing ribonucleoprotein complex / small nuclear ribonucleoprotein complex / SMN-Sm protein complex / spliceosomal tri-snRNP complex / commitment complex / P granule / U4 snRNP / snRNP binding / U2-type precatalytic spliceosome / mRNA cis splicing, via spliceosome / U2-type prespliceosome assembly / U2-type catalytic step 2 spliceosome / U2-type spliceosomal complex / U4 snRNA binding / telomerase holoenzyme complex / telomerase RNA binding / box C/D snoRNP assembly / U1 snRNP / U3 snoRNA binding / U2 snRNP / spliceosome conformational change to release U4 (or U4atac) and U1 (or U11) / RNA Polymerase II Transcription Termination / protein methylation / regulation of protein catabolic process / U2-type prespliceosome / K63-linked polyubiquitin modification-dependent protein binding / mRNA modification / precatalytic spliceosome / rRNA modification in the nucleus and cytosol / mRNA Splicing - Minor Pathway / MLL1 complex / spliceosomal complex assembly / negative regulation of mRNA splicing, via spliceosome / spliceosomal tri-snRNP complex assembly / U5 snRNP / U5 snRNA binding / pre-mRNA intronic binding / U2 snRNA binding / U6 snRNA binding / U4/U6 x U5 tri-snRNP complex / ribonucleoprotein complex binding / protein deubiquitination / Cajal body / U1 snRNA binding / RNA processing / Major pathway of rRNA processing in the nucleolus and cytosol / spliceosomal snRNP assembly / positive regulation of protein export from nucleus / catalytic step 2 spliceosome / spliceosomal complex / mRNA Splicing - Major Pathway / RNA splicing / response to glucocorticoid / helicase activity / cellular response to xenobiotic stimulus / cellular response to tumor necrosis factor / maturation of SSU-rRNA / response to cocaine / small-subunit processome / mRNA splicing, via spliceosome / osteoblast differentiation / mRNA processing / kinetochore / mRNA Polyadenylation / neuron differentiation / cellular response to lipopolysaccharide
Similarity search - Function
CCDC84-like / Coiled coil protein 84 / Programmed cell death protein 7 / snRNP35, RNA recognition motif / : / Programmed cell death protein 7 / U11/U12 small nuclear ribonucleoprotein 25kDa protein / SNRNP25, ubiquitin-like domain / Ubiquitin-like domain / : ...CCDC84-like / Coiled coil protein 84 / Programmed cell death protein 7 / snRNP35, RNA recognition motif / : / Programmed cell death protein 7 / U11/U12 small nuclear ribonucleoprotein 25kDa protein / SNRNP25, ubiquitin-like domain / Ubiquitin-like domain / : / TRM13/UPF0224 family, U11-48K-like CHHC zinc finger domain / U11-48K-like CHHC zinc finger / Zinc finger CHHC U11-48K-type profile. / Serine/threonine-protein PRP4, catalytic domain / USP39 / : / DDX23-like domain / : / PRP4-like superfamily / pre-mRNA processing factor 4 (PRP4) like / U1-C, C2H2-type zinc finger / U1 zinc finger / SNU66/SART1 family / HIND motif / SART-1 family / HIND motif / PWI domain superfamily / PWI domain / PWI domain profile. / Pre-mRNA processing factor 4 (PRP4)-like / Splicing Factor Motif, present in Prp18 and Pr04 / Pre-mRNA-splicing factor 3 / U4/U6 small nuclear ribonucleoprotein Prp3 / pre-mRNA processing factor 3 domain / Prp31 C-terminal / U4/U6 small nuclear ribonucleoprotein Prp31 / Prp31 C terminal domain / Small nuclear ribonucleoprotein Prp3, C-terminal domain / Small nuclear ribonucleoprotein Prp3, C-terminal domain / PWI domain / PWI, domain in splicing factors / Dim1 family / Mitosis protein DIM1 / Mitosis protein DIM1 / PRP1 splicing factor, N-terminal / PRP1 splicing factor, N-terminal / Zinc finger C-x8-C-x5-C-x3-H type (and similar) / : / : / : / Small ribonucleoprotein associated, SmB/SmN / Small nuclear ribonucleoprotein D1 / Ubiquitin Carboxyl-terminal Hydrolase-like zinc finger / Zinc finger, UBP-type / Zn-finger in ubiquitin-hydrolases and other protein / Zinc finger UBP-type profile. / Brr2, N-terminal helicase PWI domain / : / N-terminal helicase PWI domain / Pre-mRNA-splicing helicase BRR2 plug domain / : / Winged Helix-turn-helix domain / Sec63 Brl domain / Zinc finger, CCCH-type superfamily / NOSIC / NOSIC (NUC001) domain / Nop domain / Nop domain superfamily / Nop, C-terminal domain / snoRNA binding domain, fibrillarin / Nop domain profile. / H/ACA ribonucleoprotein complex, subunit Nhp2-like / : / zinc finger / Tetratricopeptide repeat / Pre-mRNA-splicing factor Syf1-like / 116kDa U5 small nuclear ribonucleoprotein component, N-terminal / 116kDa U5 small nuclear ribonucleoprotein component, C-terminal / Snu114, GTP-binding domain / 116 kDa U5 small nuclear ribonucleoprotein component N-terminus / Sec63 domain / Sec63 Brl domain / : / Nucleolar protein 10-like, N-terminal domain / Small nuclear ribonucleoprotein Sm D2 / Small nuclear ribonucleoprotein Sm D3 / Small nuclear ribonucleoprotein E / Small nuclear ribonucleoprotein G / Small nuclear ribonucleoprotein F / Sm-like protein Lsm7/SmG / Like-Sm (LSM) domain containing protein, LSm4/SmD1/SmD3 / Sm-like protein Lsm6/SmF / Zinc finger, CCCH-type / Zinc finger C3H1-type profile. / LSM domain / LSM domain, eukaryotic/archaea-type / snRNP Sm proteins / Tetratricopeptide repeat / Peptidase C19, ubiquitin carboxyl-terminal hydrolase / Ubiquitin carboxyl-terminal hydrolase
Similarity search - Domain/homology
U4/U6 small nuclear ribonucleoprotein Prp4 / U4/U6.U5 tri-snRNP-associated protein 1 / U4/U6 small nuclear ribonucleoprotein Prp3 / U5 small nuclear ribonucleoprotein 200 kDa helicase / Pre-mRNA-processing factor 6 / Small nuclear ribonucleoprotein-associated proteins B and B' / NHP2-like protein 1 / Small nuclear ribonucleoprotein E / Small nuclear ribonucleoprotein F / Small nuclear ribonucleoprotein G ...U4/U6 small nuclear ribonucleoprotein Prp4 / U4/U6.U5 tri-snRNP-associated protein 1 / U4/U6 small nuclear ribonucleoprotein Prp3 / U5 small nuclear ribonucleoprotein 200 kDa helicase / Pre-mRNA-processing factor 6 / Small nuclear ribonucleoprotein-associated proteins B and B' / NHP2-like protein 1 / Small nuclear ribonucleoprotein E / Small nuclear ribonucleoprotein F / Small nuclear ribonucleoprotein G / Small nuclear ribonucleoprotein Sm D1 / Small nuclear ribonucleoprotein Sm D2 / Small nuclear ribonucleoprotein Sm D3 / Serine/threonine-protein kinase PRP4 homolog / 116 kDa U5 small nuclear ribonucleoprotein component / U11/U12 small nuclear ribonucleoprotein 35 kDa protein / Ubiquitin carboxyl-terminal hydrolase 39 / U11/U12 small nuclear ribonucleoprotein 48 kDa protein / Pre-mRNA-processing-splicing factor 8 / Centrosomal AT-AC splicing factor / Programmed cell death protein 7 / U4/U6 small nuclear ribonucleoprotein Prp31 / U5 small nuclear ribonucleoprotein 40 kDa protein / Probable ATP-dependent RNA helicase DDX23 / U11/U12 small nuclear ribonucleoprotein 25 kDa protein / Thioredoxin-like protein 4B / Zinc finger matrin-type protein 5
Similarity search - Component
Biological speciesHomo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.38 Å
AuthorsBai R / Yuan M / Zhang P / Luo T / Shi Y / Wan R
Funding support China, 3 items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC)32171214 China
National Natural Science Foundation of China (NSFC)31930059 China
National Natural Science Foundation of China (NSFC)32100987 China
CitationJournal: Science / Year: 2024
Title: Structural basis of U12-type intron engagement by the fully assembled human minor spliceosome.
Authors: Rui Bai / Meng Yuan / Pu Zhang / Ting Luo / Yigong Shi / Ruixue Wan /
Abstract: The minor spliceosome, which is responsible for the splicing of U12-type introns, comprises five small nuclear RNAs (snRNAs), of which only one is shared with the major spliceosome. In this work, we ...The minor spliceosome, which is responsible for the splicing of U12-type introns, comprises five small nuclear RNAs (snRNAs), of which only one is shared with the major spliceosome. In this work, we report the 3.3-angstrom cryo-electron microscopy structure of the fully assembled human minor spliceosome pre-B complex. The atomic model includes U11 small nuclear ribonucleoprotein (snRNP), U12 snRNP, and U4atac/U6atac.U5 tri-snRNP. U11 snRNA is recognized by five U11-specific proteins (20K, 25K, 35K, 48K, and 59K) and the heptameric Sm ring. The 3' half of the 5'-splice site forms a duplex with U11 snRNA; the 5' half is recognized by U11-35K, U11-48K, and U11 snRNA. Two proteins, CENATAC and DIM2/TXNL4B, specifically associate with the minor tri-snRNP. A structural analysis uncovered how two conformationally similar tri-snRNPs are differentiated by the minor and major prespliceosomes for assembly.
History
DepositionFeb 2, 2024-
Header (metadata) releaseMar 20, 2024-
Map releaseMar 20, 2024-
UpdateMar 27, 2024-
Current statusMar 27, 2024Processing site: PDBc / Status: Released

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Structure visualization

Supplemental images

emd_38993.png

Downloads & links

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Map

FileDownload / File: emd_38993.map.gz / Format: CCP4 / Size: 512 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

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AxesZ (Sec.)Y (Row.)X (Col.)
1.07 Å/pix.
x 512 pix.
= 549.99 Å		1.07 Å/pix.
x 512 pix.
= 549.99 Å		1.07 Å/pix.
x 512 pix.
= 549.99 Å

Surface

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Images are generated by Spider.

Voxel sizeX=Y=Z: 1.0742 Å
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Histogram (log scale)
Contour LevelBy AUTHOR: 0.25
Minimum - Maximum-1.1841499 - 2.669018
Average (Standard dev.)0.003640113 (±0.048953224)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions512512512
Spacing512512512
CellA=B=C: 549.9904 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #2

Fileemd_38993_half_map_1.map
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Half map: #1

Fileemd_38993_half_map_2.map
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Sample components

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Entire : human minor pre-B complex

EntireName: human minor pre-B complex
Components
  • Complex: human minor pre-B complex
    • RNA: pre-mRNA
    • RNA: U5 snRNA
    • Protein or peptide: Pre-mRNA-processing-splicing factor 8
    • Protein or peptide: 116 kDa U5 small nuclear ribonucleoprotein component
    • Protein or peptide: U5 small nuclear ribonucleoprotein 200 kDa helicase
    • Protein or peptide: U5 small nuclear ribonucleoprotein 40 kDa protein
    • Protein or peptide: Pre-mRNA-processing factor 6
    • Protein or peptide: Thioredoxin-like protein 4B
    • Protein or peptide: Probable ATP-dependent RNA helicase DDX23
    • RNA: U4atac snRNA
    • RNA: U6atac snRNA
    • Protein or peptide: U4/U6 small nuclear ribonucleoprotein Prp31
    • Protein or peptide: U4/U6 small nuclear ribonucleoprotein Prp4
    • Protein or peptide: U4/U6 small nuclear ribonucleoprotein Prp3
    • Protein or peptide: NHP2-like protein 1
    • RNA: U11 snRNA
    • Protein or peptide: Serine/threonine-protein kinase PRP4 homolog
    • Protein or peptide: Centrosomal AT-AC splicing factor
    • Protein or peptide: U4/U6.U5 tri-snRNP-associated protein 1
    • Protein or peptide: U4/U6.U5 tri-snRNP-associated protein 2
    • Protein or peptide: Zinc finger matrin-type protein 5
    • Protein or peptide: U11/U12 small nuclear ribonucleoprotein 35 kDa protein
    • Protein or peptide: U11/U12 small nuclear ribonucleoprotein 25 kDa protein
    • Protein or peptide: U11/U12 small nuclear ribonucleoprotein 48 kDa protein
    • Protein or peptide: Programmed cell death protein 7
    • Protein or peptide: Small nuclear ribonucleoprotein-associated proteins B and B'
    • Protein or peptide: Small nuclear ribonucleoprotein Sm D1
    • Protein or peptide: Small nuclear ribonucleoprotein Sm D2
    • Protein or peptide: Small nuclear ribonucleoprotein Sm D3
    • Protein or peptide: Small nuclear ribonucleoprotein E
    • Protein or peptide: Small nuclear ribonucleoprotein F
    • Protein or peptide: Small nuclear ribonucleoprotein G
  • Ligand: MAGNESIUM ION
  • Ligand: INOSITOL HEXAKISPHOSPHATE
  • Ligand: GUANOSINE-5'-TRIPHOSPHATE
  • Ligand: ZINC ION

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Supramolecule #1: human minor pre-B complex

SupramoleculeName: human minor pre-B complex / type: complex / ID: 1 / Parent: 0
Macromolecule list: #1-#9, #17-#22, #24, #32, #23, #30-#31, #25-#29, #10-#16
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 1.8 MDa

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Macromolecule #1: pre-mRNA

MacromoleculeName: pre-mRNA / type: rna / ID: 1 / Number of copies: 1
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 89.522656 KDa
SequenceString: GAAUACACGG AAUUCGAGCU CGCCCACUCU UGGAUCGGAA ACCCGUCGGC CUCCGAAAUA UCCUUUUCUA GCAUGUAGAA CUGGUUACC UGCAGCCCAA GCUUGCUGCG AUAUCCGUAC ACCAUCAGGG UACGAGCUAG CCCAUGGCGU ACACCAUCAG G GUACGACU ...String:
GAAUACACGG AAUUCGAGCU CGCCCACUCU UGGAUCGGAA ACCCGUCGGC CUCCGAAAUA UCCUUUUCUA GCAUGUAGAA CUGGUUACC UGCAGCCCAA GCUUGCUGCG AUAUCCGUAC ACCAUCAGGG UACGAGCUAG CCCAUGGCGU ACACCAUCAG G GUACGACU AGUAGAUCUC GUACACCAUC AGGGUACGGA AUUCUCUAGA CACGUCUAGG GCGCAGUAGU CCAGGGUUUC CU UGAUGAU GUCAUAUCCU UAACUCAUUG UCCCUUUUUU UUC

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Macromolecule #2: U5 snRNA

MacromoleculeName: U5 snRNA / type: rna / ID: 2 / Number of copies: 1
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 37.254855 KDa
SequenceString:
AUACUCUGGU UUCUCUUCAG AUCGCAUAAA UCUUUCGCCU UUUACUAAAG AUUUCCGUGG AGAGGAACAA CUCUGAGUCU UAACCCAAU UUUUUGAGGC CUUGCUUUGG CAAGGCUA

GENBANK: GENBANK: AC105129.4

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Macromolecule #17: U4atac snRNA

MacromoleculeName: U4atac snRNA / type: rna / ID: 17 / Number of copies: 1
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 42.049789 KDa
SequenceString:
(M7M)AACCAUCCU UUUCUUGGGG UUGCGCUACU GUCCAAUGAG CGCAUAGUGA GGGCAGUACU GCUAACGCCU GAACAA CAC ACCCGCAUCA ACUAGAGCUU UUGCUUUAUU UUGGUGCAAU UUUUGGAAAA AU

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Macromolecule #18: U6atac snRNA

MacromoleculeName: U6atac snRNA / type: rna / ID: 18 / Number of copies: 1
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 40.221832 KDa
SequenceString:
GUGUUGUAUG AAAGGAGAGA AGGUUAGCAC UCCCCUUGAC AAGGAUGGAA GAGGCCCUCG GGCCUGACAA CACGCAUACG GUUAAGGCA UUGCCACCUA CUUCGUGGCA UCUAACCAUC GUUUUU

GENBANK: GENBANK: NR_023344.1

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Macromolecule #24: U11 snRNA

MacromoleculeName: U11 snRNA / type: rna / ID: 24 / Number of copies: 1
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 43.505629 KDa
SequenceString:
AAAAAGGGCU UCUGUCGUGA GUGGCACACG UAGGGCAACU CGAUUGCUCU GCGUGCGGAA UCGACAUCAA GAGAUUUCGG AAGCAUAAU UUUUUGGUAU UUGGGCAGCU GGUGAUCGUU GGUCCCGGCG CCCUUU

GENBANK: GENBANK: NR_004407.1

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Macromolecule #3: Pre-mRNA-processing-splicing factor 8

MacromoleculeName: Pre-mRNA-processing-splicing factor 8 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 273.97425 KDa
SequenceString: MAGVFPYRGP GNPVPGPLAP LPDYMSEEKL QEKARKWQQL QAKRYAEKRK FGFVDAQKED MPPEHVRKII RDHGDMTNRK FRHDKRVYL GALKYMPHAV LKLLENMPMP WEQIRDVPVL YHITGAISFV NEIPWVIEPV YISQWGSMWI MMRREKRDRR H FKRMRFPP ...String:
MAGVFPYRGP GNPVPGPLAP LPDYMSEEKL QEKARKWQQL QAKRYAEKRK FGFVDAQKED MPPEHVRKII RDHGDMTNRK FRHDKRVYL GALKYMPHAV LKLLENMPMP WEQIRDVPVL YHITGAISFV NEIPWVIEPV YISQWGSMWI MMRREKRDRR H FKRMRFPP FDDEEPPLDY ADNILDVEPL EAIQLELDPE EDAPVLDWFY DHQPLRDSRK YVNGSTYQRW QFTLPMMSTL YR LANQLLT DLVDDNYFYL FDLKAFFTSK ALNMAIPGGP KFEPLVRDIN LQDEDWNEFN DINKIIIRQP IRTEYKIAFP YLY NNLPHH VHLTWYHTPN VVFIKTEDPD LPAFYFDPLI NPISHRHSVK SQEPLPDDDE EFELPEFVEP FLKDTPLYTD NTAN GIALL WAPRPFNLRS GRTRRALDIP LVKNWYREHC PAGQPVKVRV SYQKLLKYYV LNALKHRPPK AQKKRYLFRS FKATK FFQS TKLDWVEVGL QVCRQGYNML NLLIHRKNLN YLHLDYNFNL KPVKTLTTKE RKKSRFGNAF HLCREVLRLT KLVVDS HVQ YRLGNVDAFQ LADGLQYIFA HVGQLTGMYR YKYKLMRQIR MCKDLKHLIY YRFNTGPVGK GPGCGFWAAG WRVWLFF MR GITPLLERWL GNLLARQFEG RHSKGVAKTV TKQRVESHFD LELRAAVMHD ILDMMPEGIK QNKARTILQH LSEAWRCW K ANIPWKVPGL PTPIENMILR YVKAKADWWT NTAHYNRERI RRGATVDKTV CKKNLGRLTR LYLKAEQERQ HNYLKDGPY ITAEEAVAVY TTTVHWLESR RFSPIPFPPL SYKHDTKLLI LALERLKEAY SVKSRLNQSQ REELGLIEQA YDNPHEALSR IKRHLLTQR AFKEVGIEFM DLYSHLVPVY DVEPLEKITD AYLDQYLWYE ADKRRLFPPW IKPADTEPPP LLVYKWCQGI N NLQDVWET SEGECNVMLE SRFEKMYEKI DLTLLNRLLR LIVDHNIADY MTAKNNVVIN YKDMNHTNSY GIIRGLQFAS FI VQYYGLV MDLLVLGLHR ASEMAGPPQM PNDFLSFQDI ATEAAHPIRL FCRYIDRIHI FFRFTADEAR DLIQRYLTEH PDP NNENIV GYNNKKCWPR DARMRLMKHD VNLGRAVFWD IKNRLPRSVT TVQWENSFVS VYSKDNPNLL FNMCGFECRI LPKC RTSYE EFTHKDGVWN LQNEVTKERT AQCFLRVDDE SMQRFHNRVR QILMASGSTT FTKIVNKWNT ALIGLMTYFR EAVVN TQEL LDLLVKCENK IQTRIKIGLN SKMPSRFPPV VFYTPKELGG LGMLSMGHVL IPQSDLRWSK QTDVGITHFR SGMSHE EDQ LIPNLYRYIQ PWESEFIDSQ RVWAEYALKR QEAIAQNRRL TLEDLEDSWD RGIPRINTLF QKDRHTLAYD KGWRVRT DF KQYQVLKQNP FWWTHQRHDG KLWNLNNYRT DMIQALGGVE GILEHTLFKG TYFPTWEGLF WEKASGFEES MKWKKLTN A QRSGLNQIPN RRFTLWWSPT INRANVYVGF QVQLDLTGIF MHGKIPTLKI SLIQIFRAHL WQKIHESIVM DLCQVFDQE LDALEIETVQ KETIHPRKSY KMNSSCADIL LFASYKWNVS RPSLLADSKD VMDSTTTQKY WIDIQLRWGD YDSHDIERYA RAKFLDYTT DNMSIYPSPT GVLIAIDLAY NLHSAYGNWF PGSKPLIQQA MAKIMKANPA LYVLRERIRK GLQLYSSEPT E PYLSSQNY GELFSNQIIW FVDDTNVYRV TIHKTFEGNL TTKPINGAIF IFNPRTGQLF LKIIHTSVWA GQKRLGQLAK WK TAEEVAA LIRSLPVEEQ PKQIIVTRKG MLDPLEVHLL DFPNIVIKGS ELQLPFQACL KVEKFGDLIL KATEPQMVLF NLY DDWLKT ISSYTAFSRL ILILRALHVN NDRAKVILKP DKTTITEPHH IWPTLTDEEW IKVEVQLKDL ILADYGKKNN VNVA SLTQS EIRDIILGME ISAPSQQRQQ IAEIEKQTKE QSQLTATQTR TVNKHGDEII TSTTSNYETQ TFSSKTEWRV RAISA ANLH LRTNHIYVSS DDIKETGYTY ILPKNVLKKF ICISDLRAQI AGYLYGVSPP DNPQVKEIRC IVMVPQWGTH QTVHLP GQL PQHEYLKEME PLGWIHTQPN ESPQLSPQDV TTHAKIMADN PSWDGEKTII ITCSFTPGSC TLTAYKLTPS GYEWGRQ NT DKGNNPKGYL PSHYERVQML LSDRFLGFFM VPAQSSWNYN FMGVRHDPNM KYELQLANPK EFYHEVHRPS HFLNFALL Q EGEVYSADRE DLYA

UniProtKB: Pre-mRNA-processing-splicing factor 8

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Macromolecule #4: 116 kDa U5 small nuclear ribonucleoprotein component

MacromoleculeName: 116 kDa U5 small nuclear ribonucleoprotein component / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 109.560625 KDa
SequenceString: MDTDLYDEFG NYIGPELDSD EDDDELGRET KDLDEMDDDD DDDDVGDHDD DHPGMEVVLH EDKKYYPTAE EVYGPEVETI VQEEDTQPL TEPIIKPVKT KKFTLMEQTL PVTVYEMDFL ADLMDNSELI RNVTLCGHLH HGKTCFVDCL IEQTHPEIRK R YDQDLCYT ...String:
MDTDLYDEFG NYIGPELDSD EDDDELGRET KDLDEMDDDD DDDDVGDHDD DHPGMEVVLH EDKKYYPTAE EVYGPEVETI VQEEDTQPL TEPIIKPVKT KKFTLMEQTL PVTVYEMDFL ADLMDNSELI RNVTLCGHLH HGKTCFVDCL IEQTHPEIRK R YDQDLCYT DILFTEQERG VGIKSTPVTV VLPDTKGKSY LFNIMDTPGH VNFSDEVTAG LRISDGVVLF IDAAEGVMLN TE RLIKHAV QERLAVTVCI NKIDRLILEL KLPPTDAYYK LRHIVDEVNG LISMYSTDEN LILSPLLGNV CFSSSQYSIC FTL GSFAKI YADTFGDINY QEFAKRLWGD IYFNPKTRKF TKKAPTSSSQ RSFVEFILEP LYKILAQVVG DVDTSLPRTL DELG IHLTK EELKLNIRPL LRLVCKKFFG EFTGFVDMCV QHIPSPKVGA KPKIEHTYTG GVDSDLGEAM SDCDPDGPLM CHTTK MYST DDGVQFHAFG RVLSGTIHAG QPVKVLGENY TLEDEEDSQI CTVGRLWISV ARYHIEVNRV PAGNWVLIEG VDQPIV KTA TITEPRGNEE AQIFRPLKFN TTSVIKIAVE PVNPSELPKM LDGLRKVNKS YPSLTTKVEE SGEHVILGTG ELYLDCV MH DLRKMYSEID IKVADPVVTF CETVVETSSL KCFAETPNKK NKITMIAEPL EKGLAEDIEN EVVQITWNRK KLGEFFQT K YDWDLLAARS IWAFGPDATG PNILVDDTLP SEVDKALLGS VKDSIVQGFQ WGTREGPLCD ELIRNVKFKI LDAVVAQEP LHRGGGQIIP TARRVVYSAF LMATPRLMEP YYFVEVQAPA DCVSAVYTVL ARRRGHVTQD APIPGSPLYT IKAFIPAIDS FGFETDLRT HTQGQAFSLS VFHHWQIVPG DPLDKSIVIR PLEPQPAPHL AREFMIKTRR RKGLSEDVSI SKFFDDPMLL E LAKQDVVL NYPM

UniProtKB: 116 kDa U5 small nuclear ribonucleoprotein component

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Macromolecule #5: U5 small nuclear ribonucleoprotein 200 kDa helicase

MacromoleculeName: U5 small nuclear ribonucleoprotein 200 kDa helicase / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO / EC number: RNA helicase
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 244.823422 KDa
SequenceString: MADVTARSLQ YEYKANSNLV LQADRSLIDR TRRDEPTGEV LSLVGKLEGT RMGDKAQRTK PQMQEERRAK RRKRDEDRHD INKMKGYTL LSEGIDEMVG IIYKPKTKET RETYEVLLSF IQAALGDQPR DILCGAADEV LAVLKNEKLR DKERRKEIDL L LGQTDDTR ...String:
MADVTARSLQ YEYKANSNLV LQADRSLIDR TRRDEPTGEV LSLVGKLEGT RMGDKAQRTK PQMQEERRAK RRKRDEDRHD INKMKGYTL LSEGIDEMVG IIYKPKTKET RETYEVLLSF IQAALGDQPR DILCGAADEV LAVLKNEKLR DKERRKEIDL L LGQTDDTR YHVLVNLGKK ITDYGGDKEI QNMDDNIDET YGVNVQFESD EEEGDEDVYG EVREEASDDD MEGDEAVVRC TL SANLVAS GELMSSKKKD LHPRDIDAFW LQRQLSRFYD DAIVSQKKAD EVLEILKTAS DDRECENQLV LLLGFNTFDF IKV LRQHRM MILYCTLLAS AQSEAEKERI MGKMEADPEL SKFLYQLHET EKEDLIREER SRRERVRQSR MDTDLETMDL DQGG EALAP RQVLDLEDLV FTQGSHFMAN KRCQLPDGSF RRQRKGYEEV HVPALKPKPF GSEEQLLPVE KLPKYAQAGF EGFKT LNRI QSKLYRAALE TDENLLLCAP TGAGKTNVAL MCMLREIGKH INMDGTINVD DFKIIYIAPM RSLVQEMVGS FGKRLA TYG ITVAELTGDH QLCKEEISAT QIIVCTPEKW DIITRKGGER TYTQLVRLII LDEIHLLHDD RGPVLEALVA RAIRNIE MT QEDVRLIGLS ATLPNYEDVA TFLRVDPAKG LFYFDNSFRP VPLEQTYVGI TEKKAIKRFQ IMNEIVYEKI MEHAGKNQ V LVFVHSRKET GKTARAIRDM CLEKDTLGLF LREGSASTEV LRTEAEQCKN LELKDLLPYG FAIHHAGMTR VDRTLVEDL FADKHIQVLV STATLAWGVN LPAHTVIIKG TQVYSPEKGR WTELGALDIL QMLGRAGRPQ YDTKGEGILI TSHGELQYYL SLLNQQLPI ESQMVSKLPD MLNAEIVLGN VQNAKDAVNW LGYAYLYIRM LRSPTLYGIS HDDLKGDPLL DQRRLDLVHT A ALMLDKNN LVKYDKKTGN FQVTELGRIA SHYYITNDTV QTYNQLLKPT LSEIELFRVF SLSSEFKNIT VREEEKLELQ KL LERVPIP VKESIEEPSA KINVLLQAFI SQLKLEGFAL MADMVYVTQS AGRLMRAIFE IVLNRGWAQL TDKTLNLCKM IDK RMWQSM CPLRQFRKLP EEVVKKIEKK NFPFERLYDL NHNEIGELIR MPKMGKTIHK YVHLFPKLEL SVHLQPITRS TLKV ELTIT PDFQWDEKVH GSSEAFWILV EDVDSEVILH HEYFLLKAKY AQDEHLITFF VPVFEPLPPQ YFIRVVSDRW LSCET QLPV SFRHLILPEK YPPPTELLDL QPLPVSALRN SAFESLYQDK FPFFNPIQTQ VFNTVYNSDD NVFVGAPTGS GKTICA EFA ILRMLLQSSE GRCVYITPME ALAEQVYMDW YEKFQDRLNK KVVLLTGETS TDLKLLGKGN IIISTPEKWD ILSRRWK QR KNVQNINLFV VDEVHLIGGE NGPVLEVICS RMRYISSQIE RPIRIVALSS SLSNAKDVAH WLGCSATSTF NFHPNVRP V PLELHIQGFN ISHTQTRLLS MAKPVYHAIT KHSPKKPVIV FVPSRKQTRL TAIDILTTCA ADIQRQRFLH CTEKDLIPY LEKLSDSTLK ETLLNGVGYL HEGLSPMERR LVEQLFSSGA IQVVVASRSL CWGMNVAAHL VIIMDTQYYN GKIHAYVDYP IYDVLQMVG HANRPLQDDE GRCVIMCQGS KKDFFKKFLY EPLPVESHLD HCMHDHFNAE IVTKTIENKQ DAVDYLTWTF L YRRMTQNP NYYNLQGISH RHLSDHLSEL VEQTLSDLEQ SKCISIEDEM DVAPLNLGMI AAYYYINYTT IELFSMSLNA KT KVRGLIE IISNAAEYEN IPIRHHEDNL LRQLAQKVPH KLNNPKFNDP HVKTNLLLQA HLSRMQLSAE LQSDTEEILS KAI RLIQAC VDVLSSNGWL SPALAAMELA QMVTQAMWSK DSYLKQLPHF TSEHIKRCTD KGVESVFDIM EMEDEERNAL LQLT DSQIA DVARFCNRYP NIELSYEVVD KDSIRSGGPV VVLVQLEREE EVTGPVIAPL FPQKREEGWW VVIGDAKSNS LISIK RLTL QQKAKVKLDF VAPATGAHNY TLYFMSDAYM GCDQEYKFSV DVKEAETDSD SD

UniProtKB: U5 small nuclear ribonucleoprotein 200 kDa helicase

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Macromolecule #6: U5 small nuclear ribonucleoprotein 40 kDa protein

MacromoleculeName: U5 small nuclear ribonucleoprotein 40 kDa protein / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 39.359492 KDa
SequenceString: MIEQQKRKGP ELPLVPVKRQ RHELLLGAGS GPGAGQQQAT PGALLQAGPP RCSSLQAPIM LLSGHEGEVY CCKFHPNGST LASAGFDRL ILLWNVYGDC DNYATLKGHS GAVMELHYNT DGSMLFSAST DKTVAVWDSE TGERVKRLKG HTSFVNSCYP A RRGPQLVC ...String:
MIEQQKRKGP ELPLVPVKRQ RHELLLGAGS GPGAGQQQAT PGALLQAGPP RCSSLQAPIM LLSGHEGEVY CCKFHPNGST LASAGFDRL ILLWNVYGDC DNYATLKGHS GAVMELHYNT DGSMLFSAST DKTVAVWDSE TGERVKRLKG HTSFVNSCYP A RRGPQLVC TGSDDGTVKL WDIRKKAAIQ TFQNTYQVLA VTFNDTSDQI ISGGIDNDIK VWDLRQNKLT YTMRGHADSV TG LSLSSEG SYLLSNAMDN TVRVWDVRPF APKERCVKIF QGNVHNFEKN LLRCSWSPDG SKIAAGSADR FVYVWDTTSR RIL YKLPGH AGSINEVAFH PDEPIIISAS SDKRLYMGEI Q

UniProtKB: U5 small nuclear ribonucleoprotein 40 kDa protein

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Macromolecule #7: Pre-mRNA-processing factor 6

MacromoleculeName: Pre-mRNA-processing factor 6 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 107.092242 KDa
SequenceString: MNKKKKPFLG MPAPLGYVPG LGRGATGFTT RSDIGPARDA NDPVDDRHAP PGKRTVGDQM KKNQAADDDD EDLNDTNYDE FNGYAGSLF SSGPYEKDDE EADAIYAALD KRMDERRKER REQREKEEIE KYRMERPKIQ QQFSDLKRKL AEVTEEEWLS I PEVGDARN ...String:
MNKKKKPFLG MPAPLGYVPG LGRGATGFTT RSDIGPARDA NDPVDDRHAP PGKRTVGDQM KKNQAADDDD EDLNDTNYDE FNGYAGSLF SSGPYEKDDE EADAIYAALD KRMDERRKER REQREKEEIE KYRMERPKIQ QQFSDLKRKL AEVTEEEWLS I PEVGDARN KRQRNPRYEK LTPVPDSFFA KHLQTGENHT SVDPRQTQFG GLNTPYPGGL NTPYPGGMTP GLMTPGTGEL DM RKIGQAR NTLMDMRLSQ VSDSVSGQTV VDPKGYLTDL NSMIPTHGGD INDIKKARLL LKSVRETNPH HPPAWIASAR LEE VTGKLQ VARNLIMKGT EMCPKSEDVW LEAARLQPGD TAKAVVAQAV RHLPQSVRIY IRAAELETDI RAKKRVLRKA LEHV PNSVR LWKAAVELEE PEDARIMLSR AVECCPTSVE LWLALARLET YENARKVLNK ARENIPTDRH IWITAAKLEE ANGNT QMVE KIIDRAITSL RANGVEINRE QWIQDAEECD RAGSVATCQA VMRAVIGIGI EEEDRKHTWM EDADSCVAHN ALECAR AIY AYALQVFPSK KSVWLRAAYF EKNHGTRESL EALLQRAVAH CPKAEVLWLM GAKSKWLAGD VPAARSILAL AFQANPN SE EIWLAAVKLE SENDEYERAR RLLAKARSSA PTARVFMKSV KLEWVQDNIR AAQDLCEEAL RHYEDFPKLW MMKGQIEE Q KEMMEKAREA YNQGLKKCPH STPLWLLLSR LEEKIGQLTR ARAILEKSRL KNPKNPGLWL ESVRLEYRAG LKNIANTLM AKALQECPNS GILWSEAIFL EARPQRRTKS VDALKKCEHD PHVLLAVAKL FWSQRKITKA REWFHRTVKI DSDLGDAWAF FYKFELQHG TEEQQEEVRK RCESAEPRHG ELWCAVSKDI ANWQKKIGDI LRLVAGRIKN TF

UniProtKB: Pre-mRNA-processing factor 6

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Macromolecule #8: Thioredoxin-like protein 4B

MacromoleculeName: Thioredoxin-like protein 4B / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 17.034611 KDa
SequenceString:
MSFLLPKLTS KKEVDQAIKS TAEKVLVLRF GRDEDPVCLQ LDDILSKTSS DLSKMAAIYL VDVDQTAVYT QYFDISYIPS TVFFFNGQH MKVDYGSPDH TKFVGSFKTK QDFIDLIEVI YRGAMRGKLI VQSPIDPKNI PKYDLLYQDI

UniProtKB: Thioredoxin-like protein 4B

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Macromolecule #9: Probable ATP-dependent RNA helicase DDX23

MacromoleculeName: Probable ATP-dependent RNA helicase DDX23 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO / EC number: RNA helicase
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 95.785148 KDa
SequenceString: MAGELADKKD RDASPSKEER KRSRTPDRER DRDRDRKSSP SKDRKRHRSR DRRRGGSRSR SRSRSKSAER ERRHKERERD KERDRNKKD RDRDKDGHRR DKDRKRSSLS PGRGKDFKSR KDRDSKKDEE DEHGDKKPKA QPLSLEELLA KKKAEEEAEA K PKFLSKAE ...String:
MAGELADKKD RDASPSKEER KRSRTPDRER DRDRDRKSSP SKDRKRHRSR DRRRGGSRSR SRSRSKSAER ERRHKERERD KERDRNKKD RDRDKDGHRR DKDRKRSSLS PGRGKDFKSR KDRDSKKDEE DEHGDKKPKA QPLSLEELLA KKKAEEEAEA K PKFLSKAE REAEALKRRQ QEVEERQRML EEERKKRKQF QDLGRKMLED PQERERRERR ERMERETNGN EDEEGRQKIR EE KDKSKEL HAIKERYLGG IKKRRRTRHL NDRKFVFEWD ASEDTSIDYN PLYKERHQVQ LLGRGFIAGI DLKQQKREQS RFY GDLMEK RRTLEEKEQE EARLRKLRKK EAKQRWDDRH WSQKKLDEMT DRDWRIFRED YSITTKGGKI PNPIRSWKDS SLPP HILEV IDKCGYKEPT PIQRQAIPIG LQNRDIIGVA ETGSGKTAAF LIPLLVWITT LPKIDRIEES DQGPYAIILA PTREL AQQI EEETIKFGKP LGIRTVAVIG GISREDQGFR LRMGCEIVIA TPGRLIDVLE NRYLVLSRCT YVVLDEADRM IDMGFE PDV QKILEHMPVS NQKPDTDEAE DPEKMLANFE SGKHKYRQTV MFTATMPPAV ERLARSYLRR PAVVYIGSAG KPHERVE QK VFLMSESEKR KKLLAILEQG FDPPIIIFVN QKKGCDVLAK SLEKMGYNAC TLHGGKGQEQ REFALSNLKA GAKDILVA T DVAGRGIDIQ DVSMVVNYDM AKNIEDYIHR IGRTGRAGKS GVAITFLTKE DSAVFYELKQ AILESPVSSC PPELANHPD AQHKPGTILT KKRREETIFA

UniProtKB: Probable ATP-dependent RNA helicase DDX23

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Macromolecule #10: Small nuclear ribonucleoprotein-associated proteins B and B'

MacromoleculeName: Small nuclear ribonucleoprotein-associated proteins B and B'
type: protein_or_peptide / ID: 10 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 24.642131 KDa
SequenceString: MTVGKSSKML QHIDYRMRCI LQDGRIFIGT FKAFDKHMNL ILCDCDEFRK IKPKNSKQAE REEKRVLGLV LLRGENLVSM TVEGPPPKD TGIARVPLAG AAGGPGIGRA AGRGIPAGVP MPQAPAGLAG PVRGVGGPSQ QVMTPQGRGT VAAAAAAATA S IAGAPTQY ...String:
MTVGKSSKML QHIDYRMRCI LQDGRIFIGT FKAFDKHMNL ILCDCDEFRK IKPKNSKQAE REEKRVLGLV LLRGENLVSM TVEGPPPKD TGIARVPLAG AAGGPGIGRA AGRGIPAGVP MPQAPAGLAG PVRGVGGPSQ QVMTPQGRGT VAAAAAAATA S IAGAPTQY PPGRGGPPPP MGRGAPPPGM MGPPPGMRPP MGPPMGIPPG RGTPMGMPPP GMRPPPPGMR GPPPPGMRPP RP

UniProtKB: Small nuclear ribonucleoprotein-associated proteins B and B'

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Macromolecule #11: Small nuclear ribonucleoprotein Sm D1

MacromoleculeName: Small nuclear ribonucleoprotein Sm D1 / type: protein_or_peptide / ID: 11 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 13.310653 KDa
SequenceString:
MKLVRFLMKL SHETVTIELK NGTQVHGTIT GVDVSMNTHL KAVKMTLKNR EPVQLETLSI RGNNIRYFIL PDSLPLDTLL VDVEPKVKS KKREAVAGRG RGRGRGRGRG RGRGRGGPRR

UniProtKB: Small nuclear ribonucleoprotein Sm D1

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Macromolecule #12: Small nuclear ribonucleoprotein Sm D2

MacromoleculeName: Small nuclear ribonucleoprotein Sm D2 / type: protein_or_peptide / ID: 12 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 13.551928 KDa
SequenceString:
MSLLNKPKSE MTPEELQKRE EEEFNTGPLS VLTQSVKNNT QVLINCRNNK KLLGRVKAFD RHCNMVLENV KEMWTEVPKS GKGKKKSKP VNKDRYISKM FLRGDSVIVV LRNPLIAGK

UniProtKB: Small nuclear ribonucleoprotein Sm D2

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Macromolecule #13: Small nuclear ribonucleoprotein Sm D3

MacromoleculeName: Small nuclear ribonucleoprotein Sm D3 / type: protein_or_peptide / ID: 13 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 13.940308 KDa
SequenceString:
MSIGVPIKVL HEAEGHIVTC ETNTGEVYRG KLIEAEDNMN CQMSNITVTY RDGRVAQLEQ VYIRGSKIRF LILPDMLKNA PMLKSMKNK NQGSGAGRGK AAILKAQVAA RGRGRGMGRG NIFQKRR

UniProtKB: Small nuclear ribonucleoprotein Sm D3

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Macromolecule #14: Small nuclear ribonucleoprotein E

MacromoleculeName: Small nuclear ribonucleoprotein E / type: protein_or_peptide / ID: 14 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 10.817601 KDa
SequenceString:
MAYRGQGQKV QKVMVQPINL IFRYLQNRSR IQVWLYEQVN MRIEGCIIGF DEYMNLVLDD AEEIHSKTKS RKQLGRIMLK GDNITLLQS VSN

UniProtKB: Small nuclear ribonucleoprotein E

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Macromolecule #15: Small nuclear ribonucleoprotein F

MacromoleculeName: Small nuclear ribonucleoprotein F / type: protein_or_peptide / ID: 15 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 9.734171 KDa
SequenceString:
MSLPLNPKPF LNGLTGKPVM VKLKWGMEYK GYLVSVDGYM NMQLANTEEY IDGALSGHLG EVLIRCNNVL YIRGVEEEEE DGEMRE

UniProtKB: Small nuclear ribonucleoprotein F

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Macromolecule #16: Small nuclear ribonucleoprotein G

MacromoleculeName: Small nuclear ribonucleoprotein G / type: protein_or_peptide / ID: 16 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 8.508084 KDa
SequenceString:
MSKAHPPELK KFMDKKLSLK LNGGRHVQGI LRGFDPFMNL VIDECVEMAT SGQQNNIGMV VIRGNSIIML EALERV

UniProtKB: Small nuclear ribonucleoprotein G

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Macromolecule #19: U4/U6 small nuclear ribonucleoprotein Prp31

MacromoleculeName: U4/U6 small nuclear ribonucleoprotein Prp31 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 55.528969 KDa
SequenceString: MSLADELLAD LEEAAEEEEG GSYGEEEEEP AIEDVQEETQ LDLSGDSVKT IAKLWDSKMF AEIMMKIEEY ISKQAKASEV MGPVEAAPE YRVIVDANNL TVEIENELNI IHKFIRDKYS KRFPELESLV PNALDYIRTV KELGNSLDKC KNNENLQQIL T NATIMVVS ...String:
MSLADELLAD LEEAAEEEEG GSYGEEEEEP AIEDVQEETQ LDLSGDSVKT IAKLWDSKMF AEIMMKIEEY ISKQAKASEV MGPVEAAPE YRVIVDANNL TVEIENELNI IHKFIRDKYS KRFPELESLV PNALDYIRTV KELGNSLDKC KNNENLQQIL T NATIMVVS VTASTTQGQQ LSEEELERLE EACDMALELN ASKHRIYEYV ESRMSFIAPN LSIIIGASTA AKIMGVAGGL TN LSKMPAC NIMLLGAQRK TLSGFSSTSV LPHTGYIYHS DIVQSLPPDL RRKAARLVAA KCTLAARVDS FHESTEGKVG YEL KDEIER KFDKWQEPPP VKQVKPLPAP LDGQRKKRGG RRYRKMKERL GLTEIRKQAN RMSFGEIEED AYQEDLGFSL GHLG KSGSG RVRQTQVNEA TKARISKTLQ RTLQKQSVVY GGKSTIRDRS SGTASSVAFT PLQGLEIVNP QAAEKKVAEA NQKYF SSMA EFLKVKGEKS GLMST

UniProtKB: U4/U6 small nuclear ribonucleoprotein Prp31

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Macromolecule #20: U4/U6 small nuclear ribonucleoprotein Prp4

MacromoleculeName: U4/U6 small nuclear ribonucleoprotein Prp4 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 58.536105 KDa
SequenceString: MASSRASSTQ ATKTKAPDDL VAPVVKKPHI YYGSLEEKER ERLAKGESGI LGKDGLKAGI EAGNINITSG EVFEIEEHIS ERQAEVLAE FERRKRARQI NVSTDDSEVK ACLRALGEPI TLFGEGPAER RERLRNILSV VGTDALKKTK KDDEKSKKSK E EYQQTWYH ...String:
MASSRASSTQ ATKTKAPDDL VAPVVKKPHI YYGSLEEKER ERLAKGESGI LGKDGLKAGI EAGNINITSG EVFEIEEHIS ERQAEVLAE FERRKRARQI NVSTDDSEVK ACLRALGEPI TLFGEGPAER RERLRNILSV VGTDALKKTK KDDEKSKKSK E EYQQTWYH EGPNSLKVAR LWIANYSLPR AMKRLEEARL HKEIPETTRT SQMQELHKSL RSLNNFCSQI GDDRPISYCH FS PNSKMLA TACWSGLCKL WSVPDCNLLH TLRGHNTNVG AIVFHPKSTV SLDPKDVNLA SCAADGSVKL WSLDSDEPVA DIE GHTVRV ARVMWHPSGR FLGTTCYDRS WRLWDLEAQE EILHQEGHSM GVYDIAFHQD GSLAGTGGLD AFGRVWDLRT GRCI MFLEG HLKEIYGINF SPNGYHIATG SGDNTCKVWD LRQRRCVYTI PAHQNLVTGV KFEPIHGNFL LTGAYDNTAK IWTHP GWSP LKTLAGHEGK VMGLDISSDG QLIATCSYDR TFKLWMAE

UniProtKB: U4/U6 small nuclear ribonucleoprotein Prp4

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Macromolecule #21: U4/U6 small nuclear ribonucleoprotein Prp3

MacromoleculeName: U4/U6 small nuclear ribonucleoprotein Prp3 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 77.669188 KDa
SequenceString: MALSKRELDE LKPWIEKTVK RVLGFSEPTV VTAALNCVGK GMDKKKAADH LKPFLDDSTL RFVDKLFEAV EEGRSSRHSK SSSDRSRKR ELKEVFGDDS EISKESSGVK KRRIPRFEEV EEEPEVIPGP PSESPGMLTK LQIKQMMEAA TRQIEERKKQ L SFISPPTP ...String:
MALSKRELDE LKPWIEKTVK RVLGFSEPTV VTAALNCVGK GMDKKKAADH LKPFLDDSTL RFVDKLFEAV EEGRSSRHSK SSSDRSRKR ELKEVFGDDS EISKESSGVK KRRIPRFEEV EEEPEVIPGP PSESPGMLTK LQIKQMMEAA TRQIEERKKQ L SFISPPTP QPKTPSSSQP ERLPIGNTIQ PSQAATFMND AIEKARKAAE LQARIQAQLA LKPGLIGNAN MVGLANLHAM GI APPKVEL KDQTKPTPLI LDEQGRTVDA TGKEIELTHR MPTLKANIRA VKREQFKQQL KEKPSEDMES NTFFDPRVSI APS QRQRRT FKFHDKGKFE KIAQRLRTKA QLEKLQAEIS QAARKTGIHT STRLALIAPK KELKEGDIPE IEWWDSYIIP NGFD LTEEN PKREDYFGIT NLVEHPAQLN PPVDNDTPVT LGVYLTKKEQ KKLRRQTRRE AQKELQEKVR LGLMPPPEPK VRISN LMRV LGTEAVQDPT KVEAHVRAQM AKRQKAHEEA NAARKLTAEQ RKVKKIKKLK EDISQGVHIS VYRVRNLSNP AKKFKI EAN AGQLYLTGVV VLHKDVNVVV VEGGPKAQKK FKRLMLHRIK WDEQTSNTKG DDDEESDEEA VKKTNKCVLV WEGTAKD RS FGEMKFKQCP TENMAREHFK KHGAEHYWDL ALSESVLEST D

UniProtKB: U4/U6 small nuclear ribonucleoprotein Prp3

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Macromolecule #22: NHP2-like protein 1

MacromoleculeName: NHP2-like protein 1 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 14.191524 KDa
SequenceString:
MTEADVNPKA YPLADAHLTK KLLDLVQQSC NYKQLRKGAN EATKTLNRGI SEFIVMAADA EPLEIILHLP LLCEDKNVPY VFVRSKQAL GRACGVSRPV IACSVTIKEG SQLKQQIQSI QQSIERLLV

UniProtKB: NHP2-like protein 1

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Macromolecule #23: Centrosomal AT-AC splicing factor

MacromoleculeName: Centrosomal AT-AC splicing factor / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 38.03416 KDa
SequenceString: MAPAQRCPLC RQTFFCGRGH VYSRKHQRQL KEALERLLPQ VEAARKAIRA AQVERYVPEH ERCCWCLCCG CEVREHLSHG NLTVLYGGL LEHLASPEHK KATNKFWWEN KAEVQMKEKF LVTPQDYARF KKSMVKGLDS YEEKEDKVIK EMAAQIREVE Q SRQEVVRS ...String:
MAPAQRCPLC RQTFFCGRGH VYSRKHQRQL KEALERLLPQ VEAARKAIRA AQVERYVPEH ERCCWCLCCG CEVREHLSHG NLTVLYGGL LEHLASPEHK KATNKFWWEN KAEVQMKEKF LVTPQDYARF KKSMVKGLDS YEEKEDKVIK EMAAQIREVE Q SRQEVVRS VLEPQAVPDP EEGSSAPRSW KGMNSQVASS LQQPSNLDLP PAPELDWMET GPSLTFIGHQ DIPGVGNIHS GA TPPWMIQ DEEYIAGNQE IGPSYEEFLK EKEKQKLKKL PPDRVGANFD HSSRTSAGWL PSFGRVWNNG RRWQSRHQFK TEA AAMKKQ SHTEKS

UniProtKB: Centrosomal AT-AC splicing factor

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Macromolecule #25: Zinc finger matrin-type protein 5

MacromoleculeName: Zinc finger matrin-type protein 5 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 20.002801 KDa
SequenceString:
MGKRYFCDYC DRSFQDNLHN RKKHLNGLQH LKAKKVWYDM FRDAAAILLD EQNKRPCRKF LLTGQCDFGS NCRFSHMSER DLQELSIQV EEERRAREWL LDAPELPEGH LEDWLEKRAK RLSSAPSSRA EPIRTTVFQY PVGWPPVQEL PPSLRAPPPG G WPLQPRVQ WG

UniProtKB: Zinc finger matrin-type protein 5

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Macromolecule #26: U11/U12 small nuclear ribonucleoprotein 35 kDa protein

MacromoleculeName: U11/U12 small nuclear ribonucleoprotein 35 kDa protein
type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 29.514471 KDa
SequenceString: MNDWMPIAKE YDPLKAGSID GTDEDPHDRA VWRAMLARYV PNKGVIGDPL LTLFVARLNL QTKEDKLKEV FSRYGDIRRL RLVRDLVTG FSKGYAFIEY KEERAVIKAY RDADGLVIDQ HEIFVDYELE RTLKGWIPRR LGGGLGGKKE SGQLRFGGRD R PFRKPINL ...String:
MNDWMPIAKE YDPLKAGSID GTDEDPHDRA VWRAMLARYV PNKGVIGDPL LTLFVARLNL QTKEDKLKEV FSRYGDIRRL RLVRDLVTG FSKGYAFIEY KEERAVIKAY RDADGLVIDQ HEIFVDYELE RTLKGWIPRR LGGGLGGKKE SGQLRFGGRD R PFRKPINL PVVKNDLYRE GKRERRERSR SRERHWDSRT RDRDHDRGRE KRWQEREPTR VWPDNDWERE RDFRDDRIKG RE KKERGK

UniProtKB: U11/U12 small nuclear ribonucleoprotein 35 kDa protein

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Macromolecule #27: U11/U12 small nuclear ribonucleoprotein 25 kDa protein

MacromoleculeName: U11/U12 small nuclear ribonucleoprotein 25 kDa protein
type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 15.290729 KDa
SequenceString:
MDVFQEGLAM VVQDPLLCDL PIQVTLEEVN SQIALEYGQA MTVRVCKMDG EVMPVVVVQS ATVLDLKKAI QRYVQLKQER EGGIQHISW SYVWRTYHLT SAGEKLTEDR KKLRDYGIRN RDEVSFIKKL RQK

UniProtKB: U11/U12 small nuclear ribonucleoprotein 25 kDa protein

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Macromolecule #28: U11/U12 small nuclear ribonucleoprotein 48 kDa protein

MacromoleculeName: U11/U12 small nuclear ribonucleoprotein 48 kDa protein
type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 40.042621 KDa
SequenceString: MEGEPPPVEE RRRLQEELNE FVESGCRTLE EVTASLGWDL DSLDPGEEEA AEDEVVICPY DSNHHMPKSS LAKHMASCRL RKMGYTKEE EDEMYNPEFF YENVKIPSIT LNKDSQFQII KQARTAVGKD SDCYNQRIYS SLPVEVPLNH KRFVCDLTQA D RLALYDFV ...String:
MEGEPPPVEE RRRLQEELNE FVESGCRTLE EVTASLGWDL DSLDPGEEEA AEDEVVICPY DSNHHMPKSS LAKHMASCRL RKMGYTKEE EDEMYNPEFF YENVKIPSIT LNKDSQFQII KQARTAVGKD SDCYNQRIYS SLPVEVPLNH KRFVCDLTQA D RLALYDFV VEETKKKRSD SQIIENDSDL FVDLAAKINQ DNSRKSPKSY LEILAEVRDY KRRRQSYRAK NVHITKKSYT EV IRDVINV HMEELSNHWQ EEQEKAEDDA EKNEERRSAS VDSRQSGGSY LDAECSRHRR DRSRSPHKRK RNKDKDKNCE SRR RKERDG ERHHSHKRRK QKI

UniProtKB: U11/U12 small nuclear ribonucleoprotein 48 kDa protein

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Macromolecule #29: Programmed cell death protein 7

MacromoleculeName: Programmed cell death protein 7 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 54.791984 KDa
SequenceString: MALPPFFGQG RPGPPPPQPP PPAPFGCPPP PLPSPAFPPP LPQRPGPFPG ASAPFLQPPL ALQPRASAEA SRGGGGAGAF YPVPPPPLP PPPPQCRPFP GTDAGERPRP PPPGPGPPWS PRWPEAPPPP ADVLGDAALQ RLRDRQWLEA VFGTPRRAGC P VPQRTHAG ...String:
MALPPFFGQG RPGPPPPQPP PPAPFGCPPP PLPSPAFPPP LPQRPGPFPG ASAPFLQPPL ALQPRASAEA SRGGGGAGAF YPVPPPPLP PPPPQCRPFP GTDAGERPRP PPPGPGPPWS PRWPEAPPPP ADVLGDAALQ RLRDRQWLEA VFGTPRRAGC P VPQRTHAG PSLGEVRARL LRALRLVRRL RGLSQALREA EADGAAWVLL YSQTAPLRAE LAERLQPLTQ AAYVGEARRR LE RVRRRRL RLRERARERE AEREAEAARA VEREQEIDRW RVKCVQEVEE KKREQELKAA ADGVLSEVRK KQADTKRMVD ILR ALEKLR KLRKEAAARK GVCPPASADE TFTHHLQRLR KLIKKRSELY EAEERALRVM LEGEQEEERK RELEKKQRKE KEKI LLQKR EIESKLFGDP DEFPLAHLLE PFRQYYLQAE HSLPALIQIR HDWDQYLVPS DHPKGNFVPQ GWVLPPLPSN DIWAT AVKL H

UniProtKB: Programmed cell death protein 7

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Macromolecule #30: U4/U6.U5 tri-snRNP-associated protein 1

MacromoleculeName: U4/U6.U5 tri-snRNP-associated protein 1 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 90.414117 KDa
SequenceString: MGSSKKHRGE KEAAGTTAAA GTGGATEQPP RHREHKKHKH RSGGSGGSGG ERRKRSRERG GERGSGRRGA EAEARSSTHG RERSQAEPS ERRVKREKRD DGYEAAASSK TSSGDASSLS IEETNKLRAK LGLKPLEVNA IKKEAGTKEE PVTADVINPM A LRQREELR ...String:
MGSSKKHRGE KEAAGTTAAA GTGGATEQPP RHREHKKHKH RSGGSGGSGG ERRKRSRERG GERGSGRRGA EAEARSSTHG RERSQAEPS ERRVKREKRD DGYEAAASSK TSSGDASSLS IEETNKLRAK LGLKPLEVNA IKKEAGTKEE PVTADVINPM A LRQREELR EKLAAAKEKR LLNQKLGKIK TLGEDDPWLD DTAAWIERSR QLQKEKDLAE KRAKLLEEMD QEFGVSTLVE EE FGQRRQD LYSARDLQGL TVEHAIDSFR EGETMILTLK DKGVLQEEED VLVNVNLVDK ERAEKNVELR KKKPDYLPYA EDE SVDDLA QQKPRSILSK YDEELEGERP HSFRLEQGGT ADGLRERELE EIRAKLRLQA QSLSTVGPRL ASEYLTPEEM VTFK KTKRR VKKIRKKEKE VVVRADDLLP LGDQTQDGDF GSRLRGRGRR RVSEVEEEKE PVPQPLPSDD TRVENMDISD EEEGG APPP GSPQVLEEDE AELELQKQLE KGRRLRQLQQ LQQLRDSGEK VVEIVKKLES RQRGWEEDED PERKGAIVFN ATSEFC RTL GEIPTYGLAG NREEQEELMD FERDEERSAN GGSESDGEEN IGWSTVNLDE EKQQQDFSAS STTILDEEPI VNRGLAA AL LLCQNKGLLE TTVQKVARVK APNKSLPSAV YCIEDKMAID DKYSRREEYR GFTQDFKEKD GYKPDVKIEY VDETGRKL T PKEAFRQLSH RFHGKGSGKM KTERRMKKLD EEALLKKMSS SDTPLGTVAL LQEKQKAQKT PYIVLSGSGK SMNANTITK

UniProtKB: U4/U6.U5 tri-snRNP-associated protein 1

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Macromolecule #31: U4/U6.U5 tri-snRNP-associated protein 2

MacromoleculeName: U4/U6.U5 tri-snRNP-associated protein 2 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 65.481426 KDa
SequenceString: MSGRSKRESR GSTRGKRESE SRGSSGRVKR ERDREREPEA ASSRGSPVRV KREFEPASAR EAPASVVPFV RVKREREVDE DSEPEREVR AKNGRVDSED RRSRHCPYLD TINRSVLDFD FEKLCSISLS HINAYACLVC GKYFQGRGLK SHAYIHSVQF S HHVFLNLH ...String:
MSGRSKRESR GSTRGKRESE SRGSSGRVKR ERDREREPEA ASSRGSPVRV KREFEPASAR EAPASVVPFV RVKREREVDE DSEPEREVR AKNGRVDSED RRSRHCPYLD TINRSVLDFD FEKLCSISLS HINAYACLVC GKYFQGRGLK SHAYIHSVQF S HHVFLNLH TLKFYCLPDN YEIIDSSLED ITYVLKPTFT KQQIANLDKQ AKLSRAYDGT TYLPGIVGLN NIKANDYANA VL QALSNVP PLRNYFLEED NYKNIKRPPG DIMFLLVQRF GELMRKLWNP RNFKAHVSPH EMLQAVVLCS KKTFQITKQG DGV DFLSWF LNALHSALGG TKKKKKTIVT DVFQGSMRIF TKKLPHPDLP AEEKEQLLHN DEYQETMVES TFMYLTLDLP TAPL YKDEK EQLIIPQVPL FNILAKFNGI TEKEYKTYKE NFLKRFQLTK LPPYLIFCIK RFTKNNFFVE KNPTIVNFPI TNVDL REYL SEEVQAVHKN TTYDLIANIV HDGKPSEGSY RIHVLHHGTG KWYELQDLQV TDILPQMITL SEAYIQIWKR RDNDET NQQ GA

UniProtKB: Ubiquitin carboxyl-terminal hydrolase 39

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Macromolecule #32: Serine/threonine-protein kinase PRP4 homolog

MacromoleculeName: Serine/threonine-protein kinase PRP4 homolog / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO / EC number: non-specific serine/threonine protein kinase
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 117.264977 KDa
SequenceString: MAAAETQSLR EQPEMEDANS EKSINEENGE VSEDQSQNKH SRHKKKKHKH RSKHKKHKHS SEEDKDKKHK HKHKHKKHKR KEIIDASDK EGMSPAKRTK LDDLALLEDL EKQRALIKAE LDNELMEGKV QSGMGLILQG YESGSEEEGE IHEKARNGNR S STRSSSTK ...String:
MAAAETQSLR EQPEMEDANS EKSINEENGE VSEDQSQNKH SRHKKKKHKH RSKHKKHKHS SEEDKDKKHK HKHKHKKHKR KEIIDASDK EGMSPAKRTK LDDLALLEDL EKQRALIKAE LDNELMEGKV QSGMGLILQG YESGSEEEGE IHEKARNGNR S STRSSSTK GKLELVDNKI TTKKRSKSRS KERTRHRSDK KKSKGGIEIV KEKTTRSKSK ERKKSKSPSK RSKSQDQARK SK SPTLRRR SQEKIGKARS PTDDKVKIED KSKSKDRKKS PIINESRSRD RGKKSRSPVD LRGKSKDRRS RSKERKSKRS ETD KEKKPI KSPSKDASSG KENRSPSRRP GRSPKRRSLS PKPRDKSRRS RSPLLNDRRS KQSKSPSRTL SPGRRAKSRS LERK RREPE RRRLSSPRTR PRDDILSRRE RSKDASPINR WSPTRRRSRS PIRRRSRSPL RRSRSPRRRS RSPRRRDRGR RSRSR LRRR SRSRGGRRRR SRSKVKEDKF KGSLSEGMKV EQESSSDDNL EDFDVEEEDE EALIEQRRIQ RQAIVQKYKY LAEDSN MSV PSEPSSPQSS TRTRSPSPDD ILERVAADVK EYERENVDTF EASVKAKHNL MTVEQNNGSS QKKLLAPDMF TESDDMF AA YFDSARLRAA GIGKDFKENP NLRDNWTDAE GYYRVNIGEV LDKRYNVYGY TGQGVFSNVV RARDNARANQ EVAVKIIR N NELMQKTGLK ELEFLKKLND ADPDDKFHCL RLFRHFYHKQ HLCLVFEPLS MNLREVLKKY GKDVGLHIKA VRSYSQQLF LALKLLKRCN ILHADIKPDN ILVNESKTIL KLCDFGSASH VADNDITPYL VSRFYRAPEI IIGKSYDYGI DMWSVGCTLY ELYTGKILF PGKTNNHMLK LAMDLKGKMP NKMIRKGVFK DQHFDQNLNF MYIEVDKVTE REKVTVMSTI NPTKDLLADL I GCQRLPED QRKKVHQLKD LLDQILMLDP AKRISINQAL QHAFIQEKI

UniProtKB: Serine/threonine-protein kinase PRP4 homolog

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Macromolecule #33: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 33 / Number of copies: 2 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Macromolecule #34: INOSITOL HEXAKISPHOSPHATE

MacromoleculeName: INOSITOL HEXAKISPHOSPHATE / type: ligand / ID: 34 / Number of copies: 1 / Formula: IHP
Molecular weightTheoretical: 660.035 Da
Chemical component information

ChemComp-IHP:
INOSITOL HEXAKISPHOSPHATE

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Macromolecule #35: GUANOSINE-5'-TRIPHOSPHATE

MacromoleculeName: GUANOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 35 / Number of copies: 1 / Formula: GTP
Molecular weightTheoretical: 523.18 Da
Chemical component information

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

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Macromolecule #36: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 36 / Number of copies: 6 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.9
GridModel: Quantifoil R1.2/1.3 / Material: GOLD / Mesh: 300 / Support film - Material: CARBON / Support film - topology: CONTINUOUS / Pretreatment - Type: PLASMA CLEANING
VitrificationCryogen name: NITROGEN

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 50.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 3.0 µm / Nominal defocus min: 0.5 µm / Nominal magnification: 81000
Sample stageCooling holder cryogen: NITROGEN
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Startup modelType of model: INSILICO MODEL
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.38 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 388888
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD

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