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Open data
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Basic information
Entry | ![]() | |||||||||
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Title | Pre-translocated Pol IV transcription elongation complex | |||||||||
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![]() | TRANSCRIPTION/DNA/RNA / elongation / Pol IV / RNA polymerase / TRANSCRIPTION-DNA-RNA complex | |||||||||
Function / homology | ![]() stomatal complex patterning / siRNA-mediated long-distance post-transcriptional gene silencing / RNA polymerase IV complex / RNA polymerase V complex / gene silencing by siRNA-directed DNA methylation / stomatal complex development / siRNA-mediated retrotransposon silencing by heterochromatin formation / siRNA transcription / DNA/RNA hybrid binding / regulatory ncRNA-mediated post-transcriptional gene silencing ...stomatal complex patterning / siRNA-mediated long-distance post-transcriptional gene silencing / RNA polymerase IV complex / RNA polymerase V complex / gene silencing by siRNA-directed DNA methylation / stomatal complex development / siRNA-mediated retrotransposon silencing by heterochromatin formation / siRNA transcription / DNA/RNA hybrid binding / regulatory ncRNA-mediated post-transcriptional gene silencing / siRNA processing / regulatory ncRNA-mediated gene silencing / RNA polymerase complex / RNA polymerase II activity / regulation of immune response / defense response to fungus / heterochromatin / RNA polymerase II, core complex / DNA-templated transcription initiation / ribonucleoside binding / DNA-directed 5'-3' RNA polymerase activity / DNA-directed RNA polymerase / nucleic acid binding / transcription by RNA polymerase II / single-stranded RNA binding / protein dimerization activity / RNA-directed RNA polymerase / RNA-dependent RNA polymerase activity / DNA-templated transcription / nucleotide binding / nucleolus / DNA binding / zinc ion binding / nucleoplasm / nucleus / metal ion binding / cytoplasm Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.4 Å | |||||||||
![]() | Huang K / Fang CL / Zhang Y | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Transcription of the Plant RNA polymerase IV is prone to backtracking Authors: Huang K / Fang CL / Zhang Y | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 45.6 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 31.2 KB 31.2 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 9.5 KB | Display | ![]() |
Images | ![]() | 106.9 KB | ||
Filedesc metadata | ![]() | 9.9 KB | ||
Others | ![]() ![]() | 84.5 MB 84.5 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8xmdMC ![]() 8xmbC ![]() 8xmcC ![]() 8xmeC M: atomic model generated by this map C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
File | ![]() | ||||||||||||||||||||
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Voxel size | X=Y=Z: 1.1 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: #2
File | emd_38472_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #1
File | emd_38472_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
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Sample components
+Entire : Pre-translocated Pol IV transcription elongation complex
+Supramolecule #1: Pre-translocated Pol IV transcription elongation complex
+Macromolecule #1: DNA-directed RNA polymerase IV subunit 1
+Macromolecule #2: DNA-directed RNA polymerases IV and V subunit 2
+Macromolecule #3: DNA-directed RNA polymerases II, IV and V subunit 3
+Macromolecule #4: DNA-directed RNA polymerases IV and V subunit 4
+Macromolecule #5: DNA-directed RNA polymerases II and IV subunit 5A
+Macromolecule #6: DNA-directed RNA polymerases II, IV and V subunit 6A
+Macromolecule #7: DNA-directed RNA polymerase IV subunit 7
+Macromolecule #8: DNA-directed RNA polymerases II, IV and V subunit 8B
+Macromolecule #9: DNA-directed RNA polymerases II, IV and V subunit 9A
+Macromolecule #10: DNA-directed RNA polymerases II, IV and V subunit 10
+Macromolecule #11: DNA-directed RNA polymerases II, IV and V subunit 11
+Macromolecule #12: DNA-directed RNA polymerases II, IV and V subunit 12
+Macromolecule #13: RNA-dependent RNA polymerase 2
+Macromolecule #14: Nontemplate_DNA
+Macromolecule #16: Template_DNA
+Macromolecule #15: RNA
+Macromolecule #17: ZINC ION
+Macromolecule #18: MAGNESIUM ION
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Concentration | 1.2 mg/mL |
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Buffer | pH: 7.5 |
Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 50.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.2 µm / Nominal defocus min: 1.2 µm |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |