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- EMDB-38424: The cryo-EM structure of Orf2971-FtsHi motor complex -

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Basic information

Entry
Database: EMDB / ID: EMD-38424
TitleThe cryo-EM structure of Orf2971-FtsHi motor complex
Map data
Sample
  • Complex: The complex of Orf2971-FtsHi motor
    • Protein or peptide: x 19 types
  • Ligand: x 6 types
KeywordsChlamydomonas reinhardtii / protein transportation / chloroplast / lipid synthesis / Enzyme / PROTEIN TRANSPORT
Function / homology
Function and homology information


fatty acid transmembrane transporter activity / chloroplast inner membrane / phytochromobilin biosynthetic process / oxidoreductase activity, acting on the CH-CH group of donors, iron-sulfur protein as acceptor / poly(ADP-ribose) glycohydrolase / cobalt ion binding / ATP-dependent peptidase activity / chloroplast thylakoid membrane / acyl carrier activity / fatty acid transport ...fatty acid transmembrane transporter activity / chloroplast inner membrane / phytochromobilin biosynthetic process / oxidoreductase activity, acting on the CH-CH group of donors, iron-sulfur protein as acceptor / poly(ADP-ribose) glycohydrolase / cobalt ion binding / ATP-dependent peptidase activity / chloroplast thylakoid membrane / acyl carrier activity / fatty acid transport / chloroplast / metalloendopeptidase activity / protein transport / oxidoreductase activity / hydrolase activity / ATP hydrolysis activity / proteolysis / ATP binding / membrane
Similarity search - Function
Tic22-like / Tic22-like family / Fatty acid desaturase / Acyl carrier protein, chloroplastic / Ferredoxin-dependent bilin reductase / Ferredoxin-dependent bilin reductase / ADP-ribosylation/Crystallin J1 / ADP-ribosylglycohydrolase / ADP-ribosylation/Crystallin J1 superfamily / : ...Tic22-like / Tic22-like family / Fatty acid desaturase / Acyl carrier protein, chloroplastic / Ferredoxin-dependent bilin reductase / Ferredoxin-dependent bilin reductase / ADP-ribosylation/Crystallin J1 / ADP-ribosylglycohydrolase / ADP-ribosylation/Crystallin J1 superfamily / : / Peptidase M41 / Peptidase M41-like / Peptidase family M41 / : / Acyl carrier protein (ACP) / AAA ATPase, AAA+ lid domain / AAA+ lid domain / ATPase, AAA-type, conserved site / AAA-protein family signature. / Phosphopantetheine attachment site / ACP-like superfamily / Carrier protein (CP) domain profile. / Phosphopantetheine binding ACP domain / ATPase family associated with various cellular activities (AAA) / ATPase, AAA-type, core / ATPases associated with a variety of cellular activities / AAA+ ATPase domain / P-loop containing nucleoside triphosphate hydrolase
Similarity search - Domain/homology
Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / 4Fe-4S ferredoxin-type domain-containing protein / Flagellar associated protein / AAA+ ATPase domain-containing protein / ADP-ribosylhydrolase ARH3 / Uncharacterized protein / Fatty acid desaturase domain-containing protein / Uncharacterized protein ...Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / 4Fe-4S ferredoxin-type domain-containing protein / Flagellar associated protein / AAA+ ATPase domain-containing protein / ADP-ribosylhydrolase ARH3 / Uncharacterized protein / Fatty acid desaturase domain-containing protein / Uncharacterized protein / AAA+ ATPase domain-containing protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized 341.7 kDa protein in psbD-psbC intergenic region / Acyl carrier protein
Similarity search - Component
Biological speciesChlamydomonas reinhardtii (plant)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.2 Å
AuthorsWang N / Li M
Funding support China, 1 items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC) China
CitationJournal: Mol Plant / Year: 2024
Title: Architecture of the ATP-driven motor for protein import into chloroplasts.
Authors: Ning Wang / Jiale Xing / Xiaodong Su / Junting Pan / Hui Chen / Lifang Shi / Long Si / Wenqiang Yang / Mei Li /
Abstract: Thousands of nuclear-encoded proteins are transported into chloroplasts through the TOC-TIC translocon that spans the chloroplast envelope membranes. A motor complex pulls the translocated proteins ...Thousands of nuclear-encoded proteins are transported into chloroplasts through the TOC-TIC translocon that spans the chloroplast envelope membranes. A motor complex pulls the translocated proteins out of the TOC-TIC complex into the chloroplast stroma by hydrolyzing ATP. The Orf2971-FtsHi complex has been suggested to serve as the ATP-hydrolyzing motor in Chlamydomonas reinhardtii, but little is known about its architecture and assembly. Here, we report the 3.2-Å resolution structure of the Chlamydomonas Orf2971-FtsHi complex. The 20-subunit complex spans the chloroplast inner envelope, with two bulky modules protruding into the intermembrane space and stromal matrix. Six subunits form a hetero-hexamer that potentially provides the pulling force through ATP hydrolysis. The remaining subunits, including potential enzymes/chaperones, likely facilitate the complex assembly and regulate its proper function. Taken together, our results provide the structural foundation for a mechanistic understanding of chloroplast protein translocation.
History
DepositionDec 24, 2023-
Header (metadata) releaseDec 4, 2024-
Map releaseDec 4, 2024-
UpdateDec 4, 2024-
Current statusDec 4, 2024Processing site: PDBj / Status: Released

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Structure visualization

Supplemental images

emd_38424.png

Downloads & links

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Map

FileDownload / File: emd_38424.map.gz / Format: CCP4 / Size: 282.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.04 Å/pix.
x 420 pix.
= 436.8 Å		1.04 Å/pix.
x 420 pix.
= 436.8 Å		1.04 Å/pix.
x 420 pix.
= 436.8 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.04 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.00943
Minimum - Maximum-0.0 - 0.09903348
Average (Standard dev.)0.00021641939 (±0.0018428217)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions420420420
Spacing420420420
CellA=B=C: 436.8 Å
α=β=γ: 90.0 °

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Supplemental data

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Sample components

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Entire : The complex of Orf2971-FtsHi motor

EntireName: The complex of Orf2971-FtsHi motor
Components
  • Complex: The complex of Orf2971-FtsHi motor
    • Protein or peptide: Ctap1
    • Protein or peptide: Fhl2
    • Protein or peptide: 4Fe-4S ferredoxin-type domain-containing protein
    • Protein or peptide: AAA+ ATPase domain-containing protein
    • Protein or peptide: Uncharacterized 341.7 kDa protein in psbD-psbC intergenic region
    • Protein or peptide: AAA+ ATPase domain-containing protein
    • Protein or peptide: Flagellar associated protein
    • Protein or peptide: Tic22
    • Protein or peptide: FaxL
    • Protein or peptide: Fatty acid desaturase domain-containing protein
    • Protein or peptide: Moc25
    • Protein or peptide: Moc29
    • Protein or peptide: Moc34
    • Protein or peptide: Moc45
    • Protein or peptide: PcyA1
    • Protein or peptide: ADP-ribosylglycohydrolase
    • Protein or peptide: Acyl carrier protein
    • Protein or peptide: Moc13
    • Protein or peptide: Moc31
  • Ligand: ZINC ION
  • Ligand: DIACYL GLYCEROL
  • Ligand: DIGALACTOSYL DIACYL GLYCEROL (DGDG)
  • Ligand: 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE
  • Ligand: ARACHIDONIC ACID
  • Ligand: 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL

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Supramolecule #1: The complex of Orf2971-FtsHi motor

SupramoleculeName: The complex of Orf2971-FtsHi motor / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#19
Source (natural)Organism: Chlamydomonas reinhardtii (plant)

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Macromolecule #1: Ctap1

MacromoleculeName: Ctap1 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 107.778039 KDa
SequenceString: MRSAELGRPP RLAQSRLRNV SSHHVTNSCL WLRPPGCRRL VASCAASKES SSASLTAERF ITDAKELNAT GSGLPIIDGP DWEEQHWAA LKAMSAGRPV ALPTPHAKFG PEDLQRIAAS GPRLEDLTLE HAERLAGPGQ LPTAPDGVAL AFRYIPRSVL G DFRQEVEP ...String:
MRSAELGRPP RLAQSRLRNV SSHHVTNSCL WLRPPGCRRL VASCAASKES SSASLTAERF ITDAKELNAT GSGLPIIDGP DWEEQHWAA LKAMSAGRPV ALPTPHAKFG PEDLQRIAAS GPRLEDLTLE HAERLAGPGQ LPTAPDGVAL AFRYIPRSVL G DFRQEVEP DWRSLPAMSP AELYAGLRAR NWTSAHYDPA AEPWRLQVFS CDYKHTGVTG WPGYRVVVTS RGGRRRWVDL AE EGELVQL TEQAPPASPA DIGYSHVFAQ LYQAYEPRYS PEALAALYGS SSSKGKAAAA AAAQHDTPAL RHLDVSYHGT GSA VAPGSG TAFLMQPSWD AVTGAIRWGL ERSGLPELRA LRDSLLPEWR PPALELNRSN NNLGVVYFAV CLTLGIVIPA LRRS RILDI RTLEEDPGAA MEFARSKSEA RKEGLTGVEF RDVAGLGPIL NEVVEVVEFL KDPGTFSKLG ARPPKGILLE GDPGT GKTL LAKALAGEAM VPFYQMSGTE FTEGIVGLGA ARVRDLFKRA RATAPCVIFV DEIDALGLRR AENDSAKTNE EREQTL NQL LTEMDGFTPD TGVVFLGATN RADLLDPALM RPGRFDRKIR MPKPDTEGRL EILKLHLRNK QVAPDVDLLQ LARDLPG LV GADLANIVNE AAMTAVRSGR QQLTARDIYA GVDRFTQGEV RPSLPTAHKL PVLCFAAKEI GIALVAGELR DRYGRVEL V ERVSIQPKGR AYSRTMFQRG TDEEYQLMTR GRLLDRIRLA LAGGFAVRTA LGEETNFTAA DIKRATRMAK KYVFYYGFS EAGGAGITTW ANQPYSGDFV IGQQRARKVV STDAMDAFAD WPTVSEDFRF DAPSPSDVTW HRYTDEVRRV LKGCSEDVLG ILAERQEAM WAGIKALSDR KELLGSELRD IFDAHPAATS RDRDARAELA AAKLDMTIFT EGANSRWPYG IEWLDDAYPK P YWVQQQEA EAAEAQAKQP AAAL

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Macromolecule #2: Fhl2

MacromoleculeName: Fhl2 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 109.355469 KDa
SequenceString: MKATGLPSLP ARALGAAGCS TSPRPAALGW SSRGCASGRR RACARVHVAD AEAVASGVAA TEAAAAVPAL PARATAVVAP LPEKNYGSL RGGRWPFLYD NVYGLPVVRQ VASYGEVLEG IRTGRISQVL WFQAPRAVTA SAAAPPPGLG GPQQPQPPPL A SPDGRCLV ...String:
MKATGLPSLP ARALGAAGCS TSPRPAALGW SSRGCASGRR RACARVHVAD AEAVASGVAA TEAAAAVPAL PARATAVVAP LPEKNYGSL RGGRWPFLYD NVYGLPVVRQ VASYGEVLEG IRTGRISQVL WFQAPRAVTA SAAAPPPGLG GPQQPQPPPL A SPDGRCLV RFANGQVKQA VIPPGEPRIS QALQQYGTAV SYIPLEPRYM PELAAMRARG AQEAVLGEVD TGAVATPVEL PE DERRGAA VGPTAFEAVA AYGSPEQLAA ALDDNYQAAA GQVAALLAER EAWVAEQEAL EAAARAERSM SDRAGGGGGG GGT ALVPSG GFSVGAWLDS IQLTNEQQAM VLKYVPILGP ILGSGFIIGL YLLARLVKGD LTDRLKMMDS EADKKKKTAL KEAR IAFLE EEVPGLVAKG ASLDDVRKRV QPVNARLGTK LAIGDGEIQS TYEACRLLLS EGVDLSAASS TAASGALAQM ESDER RAAA GAAEGGGEGG DAMNAMMEMG KLNTARIRKA TDPKIMDVKK RVRDVRRKLK RESKVQLSDE IIFFDDIAGN KQAKVE LME VVDFFRTPEK FKASGARAPK GVLLVGPPGN GKTLMARAVA GESGVAFISS SAAEFIEMYM GLGAARVRDL FNTARSV AP CIIFIDELDA VGRQRQGGGR SNDERDNTVN QLLTEMDGFE AEQQGIVVMG ATNRKDVLDA ALTRPGRFDR SIEVRRPD F QGRLEAVKVH LRDKPVAAEI DYVSLASLMG GMSGAQIAGV ANTACFLASR DGRSEVNQTD LTLAVEQAKY GRAYDQSRF VGAGRKKRFA VMEASIALAA TLLPAIEPVE YATIIPSTRS PLGRTVLKPH VGRYTTGVWT YRYLREQLLV ALAGRAGEEL VLGRDELSS LNQHRLQMAR QVAWKIMNSG MSSHPDYQHL RGLGSNYFDG SSEPGRFQQT TVVMDANQTR SEAVDADMEV E GLLNGGYK QVFELLVRNR AALDALTELL LEREKISGEE VVQVVEELGH PEDLARRAQW AGYELL

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Macromolecule #3: 4Fe-4S ferredoxin-type domain-containing protein

MacromoleculeName: 4Fe-4S ferredoxin-type domain-containing protein / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 50.682551 KDa
SequenceString: MGQFYSREFD GDPYVDLMRS LPERELVWWA QKVIWLAEGF TFVDHFARTY PRLLQHKCQR CKGAGVMTCP ACLGDARVSG GARRRAALA GLGGVAEGRS AHDHDHEAGA DGGCRVCGTA CAWDAESEWM ERWGEWESRL AYYDKATGPL MDEWYEDVLN A GNLEEDTP ...String:
MGQFYSREFD GDPYVDLMRS LPERELVWWA QKVIWLAEGF TFVDHFARTY PRLLQHKCQR CKGAGVMTCP ACLGDARVSG GARRRAALA GLGGVAEGRS AHDHDHEAGA DGGCRVCGTA CAWDAESEWM ERWGEWESRL AYYDKATGPL MDEWYEDVLN A GNLEEDTP PVEDDPPGPE VTGRWAEHDR ALHKDKKRMA ALMRRWGHPY DADANLGYQI VDPTASMGEN VWNMAQVYNS LP PELNPLR TQHLADRGGG NTQAAVEAAR SAFDAQVVME AALLQNLEAA AQDLPKPHRL PPTAGTVACN ECGGAAWGYS FFP NTAVMF GLERPFWGDT LARLSKYWNP TQVADPARTG QLLPYGEGGL RRLLAAGGGG EDEEGGALEA VVGKAPATTG RYRR DLELL LAHPELRDGA LRVPGGWGPE GGLQTYLRGQ QEEQARMQRR RDLAAEASPL ELAPAGK

UniProtKB: 4Fe-4S ferredoxin-type domain-containing protein

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Macromolecule #4: AAA+ ATPase domain-containing protein

MacromoleculeName: AAA+ ATPase domain-containing protein / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 128.535375 KDa
SequenceString: MHAQRPAGPP CSSAPSTSYP VAPSPVSSRS RGLHARRGVA EQRSLGCRST GSSDQHSNTN DGASGPSRPE QGPELDWSGL PRRQLAAMA MSPFAALSLP LVNDPAWQQS FETYGGKLRE VLLGQQEAAK NVAKQLDEGV TYMDWTYRST GVDLSAVWDP E LWIRFREA ...String:
MHAQRPAGPP CSSAPSTSYP VAPSPVSSRS RGLHARRGVA EQRSLGCRST GSSDQHSNTN DGASGPSRPE QGPELDWSGL PRRQLAAMA MSPFAALSLP LVNDPAWQQS FETYGGKLRE VLLGQQEAAK NVAKQLDEGV TYMDWTYRST GVDLSAVWDP E LWIRFREA VAQNEPAIFW NKLLDRVQYK ENLPQAGLVG DMRISYAKFL ELLKDQRVKR LVVYGDMRTA VVEVPHPWSA SV LGHPATH PFYEDSAHNR VSMLRPNPAA PEDVTQWFCA EMPEWDMEKY RFYVDLPGDF WESGVLQRHL AAQRAEGAVW DPA SGQYIL PYRAQKKVFQ VSTEVQLLDP QESWDFLGWL LAPGRLEFYE KAACVAIALR VLGIVIAIST GSPLFKLVNV GWGK LRGKG KKNATKDPKK MSKQEKKESQ WERLTSSRAR EFMTKDEKTG KMRDTGVRFE DIAGMEFLVT EMREIVRMLK GDEAY KRVG AKCPKGIIFQ GPPGTGKTYL ARAIAGEAEV PFFSSVGSEF VEMFAGVAAA RVNSLFYNAR KKAPAIIFID EIDAIG RAR STLGGDPGSM ERESALLAML VQMDGIANKT EQVLTIGATN LAQELDAALL RPGRFEVVYE VPQPGPSARM AILRYHA KG KPLEGDGQRL LLKTAEATQG WSAAALANLM NEAAILTVRR NVPAISLPMV LELVEGLNWG EQAPRIPDSE AKDRLALI T AAKAVAFALT PGLEPIKSVT MWSGRRGLGP SVDFIAMEDK AAMDMHPEET ELMGWRTNFK TNAAVVGDEP LGEFAHVAG LLVPLYAGRA AEVALFGKDG ASLATAQPLA DCFEIAYYCV RNSQVHPRFK SLPPLHTTMW LGRDDAGRWR RDPLAIGFDE ELGYHKLTL TLLKASWRRA LRLVAQRRSA ITKVAAEMLA APEEKITGAR LVEIIESTPL DDLGGEGLDG AAAAAVVEEA G NEFLPLLK EVLGQVPGII LTGESLAQTD DQGRPLPPSS ASTSSADAAA SDAAASAGPA TELRLDDATL AAVSRTLMGR LD VVDLIGR NTAVEAAERV RDALLHPETR ERLLAMRRWV EGGPGAPEFP PSPLSPEQTA AMSPSGPLYG NLALNLDWWR RRQ DNVISW SAMEILMSRR QVDLYKQDAD MTEGAIAKLG TPPAAPAAAI GSSSKSSSGQ SS

UniProtKB: AAA+ ATPase domain-containing protein

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Macromolecule #5: Uncharacterized 341.7 kDa protein in psbD-psbC intergenic region

MacromoleculeName: Uncharacterized 341.7 kDa protein in psbD-psbC intergenic region
type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 342.11675 KDa
SequenceString: MTFLNHYTYL FSIPEKQADK VSGILRLAQA RPIETLQNER INKQLNAFLK TYKFEKLITN YKKMQSFIPN NSLNGNKTNS STNKLYATS LNVFPENPPL MVRKAVSDEA DKFSKFTYSK VQVVTNNLNN GMNSKEFIKA NNLKPSLRAA ESLVLNHLTY N KFKENLYF ...String:
MTFLNHYTYL FSIPEKQADK VSGILRLAQA RPIETLQNER INKQLNAFLK TYKFEKLITN YKKMQSFIPN NSLNGNKTNS STNKLYATS LNVFPENPPL MVRKAVSDEA DKFSKFTYSK VQVVTNNLNN GMNSKEFIKA NNLKPSLRAA ESLVLNHLTY N KFKENLYF KTNNIQPTKS KSTSLFFLNI LSNSKPRTCS DFLSSPKIRK TWFRNTAWSL QTQQHRSSNG INLSLQLPYA LG PSVPAGA SGQNMYELPV AQSSSRFGTY YFLQKLLSKY LDVWNASADN GSVLSNSENI KLNFSMVSLL DSKMAIQTPN SLY FVFTQL NQKTFLSYWL LPVAGLALLT PTLLTLTGQS VSVQKFNSFI NKKTDMMVLS NTEMPSKSFG TPTLFGTSVE IYLP NSYMP KGEGESGINR VNSSINAVKK NTVTANLVLD SESQEVATSF QNDLISIKYC FNNLYNYISN KTALSTKNLF LFSAI KSNA TKHKRTQSFF SVENTTTLGN NSNFVKGHFK SSINAFSSYL PSTNVHSMIP LTSLPYLKAI SPLYSKFMID HSLKFI TPK TTLKLLQHKL NKSPKQMYTK TQNFTGLRDL RALNSFSFGQ VNFRTNHFLH SNSRPLNHYN QALKLINGYE QYKNNLQ IN CNKTLDLNTK NKLVYQVHKS HLFNQKCSQI VYKQSLYNRD LCTIRGTGTK VVDYFSHGDK LSNKNGIVLD YFVYSNLL F DNKTNTIINK DGKQNITKLK LNLTKTTVPF KTLIKKYTSI NSLVANEQTR NNLNLGLIHF NGHLSVVSNA NLLTGRPVK FIYYKFDKRL NSYLIYVNQN LKKFIQLNNN FLKPKPLSHQ KNKPVEDFNQ YATNNSSPPK TNVFEKSFVE DSSLRKPLTS LRGSKQFLN SLTILFKHQK MFKKKTLKAH KWHSDTQGIF RKHTNSSFGS ANFSNGPEES SLSTRLHIQK KRKAKKQRLE T RRQKKRTR FFPRPVWLRS RMFLNFLTER NKYYLNSTIT KQGFSLPSKD VVTTKLDWLK EDMRPSSLGA YQYKSLLTQK AG NKFQRQS FTEVVSTMEY INGIHKALNN SIFNKIVRKS LLSSSQNPLK LRLVANYSKM QFMHRVKLPF YRTLKHSEGT KNL ANKKQN LRDIKIKANY NNFKSQKANN QPQQNDKDKD KDTMFRDFWV WSYNNTQTNA FNQNLWWLLP NLTTKQSNLE FLTS TYPTA KETQRAKEEI HGNSIPTASK NQIALIRLNW ALNKTNINTF TDYSKRNNLW TTQKLRNQSK NNKTKSLEKQ FITNW EKFF LNKNLNIFSK KIISKVKQKK QKLNYMTSYL NVQSEHNVKI FHNSWWTHLN IKNLVNNQDM VIPVREGYFS VGNFNS EFI NSAIIKSINN KTLVENYVYS PSSEKETMQL LLMSSSILLH LCAIISLVSI SQVRCFVKFH LILLYKLSNV YNAILNQ LS NKLQKNLPIY NNINKLNSRY FYMNHQKSQI KQRKKLLTYF SLTLLKKQFV TVKPLQIRNF ASIKNQSSNN SNLTYTDM L PLSLRANKFR GSKYDISIRE EEGQSAHIKP SKSMYAKLNI LSLKTIFLKQ LLMNKKPSAL PSNVGLKSNR ETQKSQLIQ RIKTKELQIS LKKNIIGFSK VTKNHILKIL FNVIEVFQTA VRNISSFFEK PAEFTTTWIA YGFLVEWSSD FITIIPENVD IYIWNVFSK IYRTIPLSFI STTLGPASTV FDPVTNSTIP IQMGNFNYQK MVAFPILLSL SHLLHRRILY LFDTLFSTIT Q PDTDLIAR QEKGTLFWDI WADFLVTAAD YYNVNVAALS TIKAEQNSLI ENISNDFDNL TMSSKKPFFM PNKGVSNIKN IF WIKKLKE PQLPESIVQN REVFVRERKR TLKGLFNIYA PQEETLWNNP TSPKNLSDEK ISFKLFNQLN LQLFAEKNKI KPY FEAYFS TTQQKTNIMQ SAFPEANLNR WSVNQFITYQ SWHSHNGSNN SNGDLFIDYH PPKTFSHIPA LKYNSILQQP IGSL VCQIY SGLFNKQISK NILLVNPKTT SNNLVDYNVL LIQALAGETE MKIITDNAQR YALVNRGFAI GIKLLREVFD AIALN TPCI FLLEDIHAIG ERRPMLISDF GGGMSDDNGS FKEDFFGSQR DEVHEKNQVV YQLTRHAITH YKKPFKGDYS LAIPTN LYV TDLFLKLPTQ SISNLTNVEN HNLSIKNKIQ HNGTQSLTET KRNLGGDINK NSYLQLTQFT KTLAPPSTSP FSVLLLK EE KRLKPNKIVE ELPWTSLPGE QLATKPRTSY SVRAKVAMLA ELSLSNLSAK LDMITDLLVI IDSVRSNKGF VVFATTDI P HVLDPALRRP GRLDETICLP NIHTSNILNF TKNYEIFKSA KDTSNFGKKI ILNEMQNLTT TSTQRDMYLS CLPTNNQTH KTKREGVLTM NLKDYNILLN QVYFAEGTGG ILNSQMHKDS LQKSLNFALI SHSKKLKELN VSKLIGSNGT VSQGNVDQLG VFAGQIVNK QKKSLQQHLP NSKKSFKKKY KDKAIIYYEV GKFVLNYFLN NQLTQSSIID KPVSVTNKQT NDITIFGNDF L NLKTINYL SLYNSKNKIL LQLMLIFGGK ISQLLSSKNL VKSLKQASIN SYMVEEESGS ISSAGMPLGQ THLLPKALSV LA KPMIFSD GYNNQNLKTA TTLLLSFIHK RYLYRKNLIV PKLLSFADGN ILDEPPSPPF SSLLIPAKRF ENYKRFFRDT LTG DKMGQR KSQITLLEKL QYHMQLRSIK QLNATFSSQE NLDFQSNAAL TSQKLDTLMS LSTNNLLQNP TNINWYYQNR ILKR HGQYL TNQWWNGQLS EHNAETVFLS DIDWRSSFIK NKNINITKSK NLYRLTQQKN NTDGLDVLLD FPDTDQYYNP KRRRW LLNN GSWNFWFNFD KLYSEEIVTT WILESLIQTY KYLHKNTELL DFVTNKFITL GYIAPENANL QNISGFPSQS ELLSTK EII LTNSFKRF

UniProtKB: Uncharacterized 341.7 kDa protein in psbD-psbC intergenic region

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Macromolecule #6: AAA+ ATPase domain-containing protein

MacromoleculeName: AAA+ ATPase domain-containing protein / type: protein_or_peptide / ID: 6 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 114.826094 KDa
SequenceString: MQRRWSRAAN VRTLATSRGE QPQDSGPSTS GRAELPLDSG IGKLISTTAK AIGLVGLMAV AVLSGPTRAA HARDRLSAQP AAEALIHHQ QPYQQPHHHQ QQHRSAGAVA NPVLSDLAAA PATLEPATLE PATSTTSALT PVEAAYSAYL RRIAEAYLAE H PQMAAPEH ...String:
MQRRWSRAAN VRTLATSRGE QPQDSGPSTS GRAELPLDSG IGKLISTTAK AIGLVGLMAV AVLSGPTRAA HARDRLSAQP AAEALIHHQ QPYQQPHHHQ QQHRSAGAVA NPVLSDLAAA PATLEPATLE PATSTTSALT PVEAAYSAYL RRIAEAYLAE H PQMAAPEH AAHVARVVRS RALGTPLSFD ELMRSAVPAP GEVPNRNSRG QVAEQVRAIL DQYDREDFDL GIKQFMLEAK VK AKLEAAS RGTSRDRAAP KDYEEALAAE LFAAEEGAAP KEKAKTEDMV DDAFTTEVVE EAMALFGDAN SVKTAWRTQE VLR ELSYTQ LWALVGEGHV ARVRFYGPEK NKVMATTRAS APGGERLCKV VLPPDPELLD HLVSNGVVVD TGVTEDDRLR ASLL VQMLR YTVPFMVISG LFWMIHTWIL DPLPNKFRRQ EFIRYRREML HVASKLNFRT PAREVRIDTG SPDFIKWDDI NGIDE VKKE INEIIEYLRN PALLRSRGVA RIGGVLLAGA PGTGKTLLAK AIAAEGGVRM FTCSGTDFYD VYSGVGARRV RETFDR LRN AAPAILFIDE FDAMGAARGA QASGDESASI INELLVQMDG FEDNRGIVVL GATNRPGAID SALIRPGRFD RIIYMPL PD ALGRAKIMQV HARNKAVDPN INWYEVARAM AGFTGADVMG LMARAARMAA RQGRHAITED DIYAAMENKT MEATLEAS T AGDGGGLVGG EGVEGSPDPI PPQLRRAVSV YEAGKALLAY ITPDYEEIAR VSVCPLNVLT GFTLFVEDED KNVNAILTR SELEGRMVVH LAGRCAEKLV MGEGQMTGMG SPDLFHANLI AREMIMSMGM GRRTGPIDLL RVAATSEAAS GADTLRAGPA AADGDPFYY HTTDMSTEQA RVALAEVVEL LDAAEAKAMY GLAINWRALQ ALTQALLDRG TITGKEVAHI LESNGVIHFP D PYTTGFGW DPDGSLRYPF KPDTPPEGGS GGGGAAAEGS APQTPDLSGA RGKTWFAGTA YDAPRNADGT FKHGWHWNMP FS VKTELPD WYKKEVERYS Y

UniProtKB: AAA+ ATPase domain-containing protein

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Macromolecule #7: Flagellar associated protein

MacromoleculeName: Flagellar associated protein / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 71.675305 KDa
SequenceString: MATTSAPTSE PWTFDLVGQL RQKFGLGPEN WDRFKGQAAE VPGADVPPSG AHVTLKDLSR PAESLPARAD EAAVQAALAD DGGWVGTPD PSKYAAGTTQ LSARELQEEV AKGNVMTWKD FKQQVSGLQG PEREALLALV AQRVAAERMF FTLEDGSKVS L WDLQQYVD ...String:
MATTSAPTSE PWTFDLVGQL RQKFGLGPEN WDRFKGQAAE VPGADVPPSG AHVTLKDLSR PAESLPARAD EAAVQAALAD DGGWVGTPD PSKYAAGTTQ LSARELQEEV AKGNVMTWKD FKQQVSGLQG PEREALLALV AQRVAAERMF FTLEDGSKVS L WDLQQYVD NNPELAALAA SVRRIAVADP EDPAGRPLPG GGASGLDRSR GLTGAAHMSG QEAEELELDW GQVGRGALWR RR PTRWLLG GLDGVKDWEL EAYAHEPLAN QLLGAKYGGR DPRAVVADPA YAADVLRAGP LLGMTFVLRA ARDLPLQEVA SSW RGLLGN YLQRQAPLSL PKAVRPAHLD PTDLNGVAWP ALLSRPAAAA HAAAEAEAAG AVPDDEMGVA WRVQSGKEAA ASVA AAQQL LQSLPDALCP GPSPAAWPLT GTKLVDEGGR NWRRGGSVWV TLQPEGGVLV QAQTGGVVGE QESYLLTHVQ GQEAL AGAV MSAFMGPQPL DPELAAAARS VLLVPANGFT AANKERDPNH PLYPSFTGVR PGRAPRDVAA YTLAGGRTPL LAAGGP GEA KLASELRTVM EAALAAAARA EAEALADAAT SPSSTSSRAA PAAALAEAEA AEARRARGRA AAAAVMAEGL RRLGPDA VA MLERTAAEAE APQGGGAVVV AGAGSASGEK GVGLTSSDIF SLARTLEQE

UniProtKB: Flagellar associated protein

+
Macromolecule #8: Tic22

MacromoleculeName: Tic22 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 36.869215 KDa
SequenceString: MREGVTRWLE RTPLAQIIRN AQEGVATQQR QRDQGPWAPM LASIAMSGAG PRPQPVMDLA MAKDEVKARL APVPVYTVAN PKNEFVLVA GENNTQLGFF FFRKEDAEAL IEKIREENPR LARDSKILRV PMDNVYEVFT TPREQTGLQG IHFRFMPDMK Q VAHALQLY ...String:
MREGVTRWLE RTPLAQIIRN AQEGVATQQR QRDQGPWAPM LASIAMSGAG PRPQPVMDLA MAKDEVKARL APVPVYTVAN PKNEFVLVA GENNTQLGFF FFRKEDAEAL IEKIREENPR LARDSKILRV PMDNVYEVFT TPREQTGLQG IHFRFMPDMK Q VAHALQLY KDAGVPTRQF IGVPVFQAEG LTVTTRDMQY VPLFLCKEDL DIAVQSAYVQ RNAAQIKLYK DKADKYQADY DQ IASQLEA AANGRERGGL ESRLAKARVK LEAARDKVES VERAPLPKVE VGSFEEVVMR MTASAGNELA AWSQVMFVAP ELL RDSAGA GGKK

UniProtKB: Uncharacterized protein

+
Macromolecule #9: FaxL

MacromoleculeName: FaxL / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 39.091016 KDa
SequenceString: MSPGALYQLA PEQLRASSRR SAAATIFRRD RIRLLRCSAA AQPGEPGEAA GPSTSGSDNS NWWASINRKT GIRGPDPAPA EEHTNGPAR DIIGDRMSRR LEDINKAERQ RVWDAMRVAA AHRYAAGQMP AWFDPEWLQQ EEAPLNAMDR MRGEQRRIDE Q QQQGEASS ...String:
MSPGALYQLA PEQLRASSRR SAAATIFRRD RIRLLRCSAA AQPGEPGEAA GPSTSGSDNS NWWASINRKT GIRGPDPAPA EEHTNGPAR DIIGDRMSRR LEDINKAERQ RVWDAMRVAA AHRYAAGQMP AWFDPEWLQQ EEAPLNAMDR MRGEQRRIDE Q QQQGEASS SDKLAMEGGG GDSGAGAGGW SGGGGGGGWW REDDPYWPLR DWGDHPMRWW TLAFAAIMAA GGLATSVATG YV EPVQAGL GAGSLLALAG AAMSDARCVP GALGVKLAWA VCALIVLKEV SVGWQHKRKR RLAASAPRLE LTGLAAAALC AGY MLTDMS GLGEVALPPN PGAVFKSPDV AYRASVWQKW GYGQVQMRV

UniProtKB: Uncharacterized protein

+
Macromolecule #10: Fatty acid desaturase domain-containing protein

MacromoleculeName: Fatty acid desaturase domain-containing protein / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 70.334234 KDa
SequenceString: MNTTRQMAAS TSCSSFTSLV APGTRLRTTV RAPAMQLRNG QRESGAAGTA ASASSSSCWR LAATALGQAQ LHSGARVSCM GGVIRSPAV LLRAGVPTRL PQSAHASPLA AVQPVTSTSG SVGELGAASR RTSSGAACSS SSSGSRSAVC TSGPVRGLGR P LPSAGLRR ...String:
MNTTRQMAAS TSCSSFTSLV APGTRLRTTV RAPAMQLRNG QRESGAAGTA ASASSSSCWR LAATALGQAQ LHSGARVSCM GGVIRSPAV LLRAGVPTRL PQSAHASPLA AVQPVTSTSG SVGELGAASR RTSSGAACSS SSSGSRSAVC TSGPVRGLGR P LPSAGLRR RRGASPIAAV GTPAVGSGSG VADSGANGST GSGNGADAAA AATAAAAPSN HHPPGSSNGD NGSSAAASAS TS SATAASA SDPAPEPEPA AASTSLDGLP ETQKYVYADE WGFSRVGADF PPGSHPSLFS QLLPQALFAF DARAAVAAVA VPL AAMAAG YGWLWYMHSI APVWQQALCA ALIGTGYAGL FKVAHECAMM RFIPQMPGLQ AALGTLLMAP ALYSLPSWRL HHLH HLLHT NMLWQDVWGW HPLTKVELAD EMVRSGGSGG AAMAAARLVL TTPIKLFASV GHWLRSWDGL DLRHFHPASY VEVLS GWAA PLAFAGLVLP AVVSAGGLSG FVSCYLAPWL VFHFWLSVLS LTAHTAPHIP WRAEGDGWDA GRAAVAGTVT LRLPRP LEV LLNNANYMLP QAVAPGLPMW SAPAAYAVLA ARLGPYLTEA SMSLKLLTNH VTRWQIYDEE AHTYRPMEEV VDEIEAD LQ QLAAAAQQAQ QQLAAQDQEA RAQQVEGREE GSGGGVPAVA

UniProtKB: Fatty acid desaturase domain-containing protein

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Macromolecule #11: Moc25

MacromoleculeName: Moc25 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 29.084951 KDa
SequenceString: MRPCPAVHRR VPPGPVAGHK QLLPLQRSCR GAPLVCRTAA PMAPSWAETR SAAASAPILE PDMLPSTSAP FISPMTPYVP EEEPTRTPP SIKDTGTLRP ASEWYPQWMQ YRRREDNYVF WQDKFMRCST DIPWAEKRWT LFSTVWYLVQ QLRFVGTPPA L RYVAFLGW ...String:
MRPCPAVHRR VPPGPVAGHK QLLPLQRSCR GAPLVCRTAA PMAPSWAETR SAAASAPILE PDMLPSTSAP FISPMTPYVP EEEPTRTPP SIKDTGTLRP ASEWYPQWMQ YRRREDNYVF WQDKFMRCST DIPWAEKRWT LFSTVWYLVQ QLRFVGTPPA L RYVAFLGW RALMFQVYAA HKALVLWQCK LDAGLARIGS GGATATFSKT MALRRLHWRN SPLAEALYAL NLYKTGRVHL LP PVAKPIP RPTFFWLF

UniProtKB: Uncharacterized protein

+
Macromolecule #12: Moc29

MacromoleculeName: Moc29 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 34.185254 KDa
SequenceString: MQSAARGCHA PAGLSSSLLR SRTYVPPCAP SSSGRVVGSR HITARAAGGK GGGKDDKAKE KADFSALWAL RIKNFFSSRR KYLQQAEQV GDDDKEKAFK AEIAKEERSI RELKDELVAI SMEEIEEQEK QGDPRPALAA TDIAQARMDL QTSPLTRAVL A VVRVRELL ...String:
MQSAARGCHA PAGLSSSLLR SRTYVPPCAP SSSGRVVGSR HITARAAGGK GGGKDDKAKE KADFSALWAL RIKNFFSSRR KYLQQAEQV GDDDKEKAFK AEIAKEERSI RELKDELVAI SMEEIEEQEK QGDPRPALAA TDIAQARMDL QTSPLTRAVL A VVRVRELL RALLLLPFSA VGGAVAAWQG LFNSQRYENF LMSEGERIWA WRNRSENERW FWEVFAWDRL IFPILVIVAW EY LVPNHLV WAVLAPLALL TWMSGRLPTP ATPEFWMLAY FGFYRKVWPD AAAWLQGYVV PLMGFA

UniProtKB: Uncharacterized protein

+
Macromolecule #13: Moc34

MacromoleculeName: Moc34 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 34.163977 KDa
SequenceString: MVHVPFFGID LPEPRLAAVM PDAVYALVQG THKLGEYAHD LVFPPTPEDL RKLEQQVNAT IPREFDRVRQ RYAEGKIAND EQLSSELED ASFNWYRRQL RTSVVGATDE ELEDVAVRKL RLEPPALQAS LQERALAAAV TAAGGVDLAA EVADAAALAA L AEQEAETR ...String:
MVHVPFFGID LPEPRLAAVM PDAVYALVQG THKLGEYAHD LVFPPTPEDL RKLEQQVNAT IPREFDRVRQ RYAEGKIAND EQLSSELED ASFNWYRRQL RTSVVGATDE ELEDVAVRKL RLEPPALQAS LQERALAAAV TAAGGVDLAA EVADAAALAA L AEQEAETR RLLAARQARL ADLRKQLRPA HRQAIGTITN ASAGMLVGVL VVKSVLTLVR RLFRKKRPAG AKAPAGAAAR RQ GSVQSSA AQLQSLAAPA ARQQQQSAAA ALASAQQAAM RPQAGASASA AAAASGKAAA SKEQPKAGAA AAAADAGAKK TRI VKKR

UniProtKB: Uncharacterized protein

+
Macromolecule #14: Moc45

MacromoleculeName: Moc45 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 48.618184 KDa
SequenceString: MQSSIAHRSL AACPVQRNRF VPARCKLASN RIGPKLLSAA IAGTPEQPAT STSTPSSEAG TKDAAVVVGP ALVAKEVVST SGHSDAVPD PNFPELPLST NRAAGTQYLA IGAAYAVAAG AVAVAALQGP QLLLASPAAA DPWSSVLLGC VAATYLRAAG V FLQLKAAS ...String:
MQSSIAHRSL AACPVQRNRF VPARCKLASN RIGPKLLSAA IAGTPEQPAT STSTPSSEAG TKDAAVVVGP ALVAKEVVST SGHSDAVPD PNFPELPLST NRAAGTQYLA IGAAYAVAAG AVAVAALQGP QLLLASPAAA DPWSSVLLGC VAATYLRAAG V FLQLKAAS DAAELLCWRH QRLALTAAAY GMVAVLTQAA GLASPQLLGL QLLLSVASAA VVANVARSAW AVRPFVSSLT EG RSPGGVL AAVAGAMTGS VSTVAGLLLT TTIVVSLYGL FAAVFAPAPA LPVAVGAWPG TAAAAAVMDG SAAGLRRLAA GGL LLTAAA SHGLFDFAGS VKQQGPIDVA QAVKKMVVMD MAAPRIRTLK YFLPNPTIYS LLNLGFVAAA VLQSYFLYIA PAWG VNVNW DTALWGPMYG TAFLGLVYGL VALTKFDWSS VVDAVLRVAC WFAELTMWFW DTFVWKFSWS EKTRRA

UniProtKB: Uncharacterized protein

+
Macromolecule #15: PcyA1

MacromoleculeName: PcyA1 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 61.329266 KDa
SequenceString: MMSSIPKSIG AQRSAASTRA HALARPVVLA PAASIPARSQ GVTSTSGRCL APPPRAAAGA GAPGTAGPTN AGAAAHEVEV DAVESPLSP EDIMRLVQQH EDVAAAAESE QLVAQFRDDP QGLYEYVNRA YAEGPRRVTT PISLLQEEIT GAVTESYPAA V ANDIIGMG ...String:
MMSSIPKSIG AQRSAASTRA HALARPVVLA PAASIPARSQ GVTSTSGRCL APPPRAAAGA GAPGTAGPTN AGAAAHEVEV DAVESPLSP EDIMRLVQQH EDVAAAAESE QLVAQFRDDP QGLYEYVNRA YAEGPRRVTT PISLLQEEIT GAVTESYPAA V ANDIIGMG SWRLKDDVDP VIEFLVARLE GCWREILDTD LCLYPREKWK EQGWDLVDSM DPHQELEGFS YADIPDPAKG EA GYPRLQL ENRVYCSKVF RKLHVEVGLR QDGLQVLHVV VYPRYSYDMP IFGMDIVMVD GRVTLAVVDC CPVRADLKLQ PHY METMAL LQRTFLEGTD PALRRIPEWG SKIFSPLALC ITPSGPEELA AFAKYAVALH RAYLTMSLNA VPVVAGPGDR REAA RLQEI QDGQKRFCDN QLVNKKTRRV LEVAMGVEWT EAYMSQLMFD FDPKYEPPYF DASFEKLYTY FDENPSFGEM ADEAM ELER GAEAERANET MAAALSGRSV SREKLAMAMG FLFQNDATFR AAVQTLSGGQ VDGNIEERLT DDLMQLLERS E

UniProtKB: Uncharacterized protein

+
Macromolecule #16: ADP-ribosylglycohydrolase

MacromoleculeName: ADP-ribosylglycohydrolase / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 51.410547 KDa
SequenceString: MRQSVRSPGT RASLQSGRAA SSCLPVTPLC PLVASTTSAP ATSISSSDAL LRSTAVASTS TSSPATVPIA ARRRHQPRSR SVAAATPAA APSPAAAAAP AWRGPGEVGD EDKAVGCLLG AAVGNVLAAP YQGDRHFEVI RLRRNGVTDF WKYDIGAQPV Q YGQYTGDF ...String:
MRQSVRSPGT RASLQSGRAA SSCLPVTPLC PLVASTTSAP ATSISSSDAL LRSTAVASTS TSSPATVPIA ARRRHQPRSR SVAAATPAA APSPAAAAAP AWRGPGEVGD EDKAVGCLLG AAVGNVLAAP YQGDRHFEVI RLRRNGVTDF WKYDIGAQPV Q YGQYTGDF ANLLAVATSL SASRGVEPAH LLGALTRAYA EGGSSVEVED ASGGSSSGFL PARRYSPYDR LVMDAVLAGT DP LKVPELA ERYLAETTRR HASSSSDRPD REPHGPSDLG AAARAAPIGL AYRRAGGERL LAAVRRSLEF SHPTPLGLDA AHV VAAAAA WCGRQQPGDA VGATPAALLS HLLNDVAVTA EQCGKLRLLR DNLFQLDEVT DWRAFYAGPQ WARLTALFSR LSFH GLATA GSEFASVVLL ALLSSWGRPE QAVIVAASLG GHAPATAQTV GALAGTLYGQ SWVPERWWRG LGEGVEGEAG REAVV QAGR ALAAVELADG L

UniProtKB: ADP-ribosylhydrolase ARH3

+
Macromolecule #17: Acyl carrier protein

MacromoleculeName: Acyl carrier protein / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 12.519384 KDa
SequenceString:
MALSMIRKSA AAPVRRAAAA PVVVRGRRVI TFAAVDKAKV LEDVRSIIST QLGTELEKVA PEAKFVDLGA D(SEP)LDTV EIM MALEEKFEIA LEEEGAEKIA TVQDAADMIA AQIAAKGN

UniProtKB: Acyl carrier protein

+
Macromolecule #18: Moc13

MacromoleculeName: Moc13 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 15.265472 KDa
SequenceString:
MGGELAERKA SSASAGPRVV QMRREAIPAE LLLVKEDPSK LPAGVLQTRE QLKQAQRDIN WAGKREQVFA AVAAGWHLAS FALNLAFWG VEGMPPDRYW PTSPRIRLQI RPGRYGNMDG GQRVYMDYLA RSEGVPLN

UniProtKB: Uncharacterized protein

+
Macromolecule #19: Moc31

MacromoleculeName: Moc31 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 31.085385 KDa
SequenceString: MASTARRKPV PEAMSEDSVE QEFGGDYSEA SLEYIRALGP KKGAPFAEVD PAEQAAALPP SQAECEAAKA VLENYKRDID ALKTYRPSE QERAAIDARR ALALEATPEE LTWLKMREYY ATQRAAAADG AASTSAPGAG DAASAAADND VAALRAEMLR V AMEALGSA ...String:
MASTARRKPV PEAMSEDSVE QEFGGDYSEA SLEYIRALGP KKGAPFAEVD PAEQAAALPP SQAECEAAKA VLENYKRDID ALKTYRPSE QERAAIDARR ALALEATPEE LTWLKMREYY ATQRAAAADG AASTSAPGAG DAASAAADND VAALRAEMLR V AMEALGSA TETEELEARA RSHLAASSAG RQWSRGVAGG AGAAASGEAS AAVLAAAADA AVASVMVGTG ARYRDDYLEE ER LTRQVAK QRGQRSQAFA LSLAAAVGVS VARWWLRRGR GGAGAGGGSG GPSRSRSTQQ MQ

+
Macromolecule #20: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 20 / Number of copies: 2 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

+
Macromolecule #21: DIACYL GLYCEROL

MacromoleculeName: DIACYL GLYCEROL / type: ligand / ID: 21 / Number of copies: 1 / Formula: DGA
Molecular weightTheoretical: 625.018 Da
Chemical component information

ChemComp-DGA:
DIACYL GLYCEROL

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Macromolecule #22: DIGALACTOSYL DIACYL GLYCEROL (DGDG)

MacromoleculeName: DIGALACTOSYL DIACYL GLYCEROL (DGDG) / type: ligand / ID: 22 / Number of copies: 2 / Formula: DGD
Molecular weightTheoretical: 949.299 Da
Chemical component information

ChemComp-DGD:
DIGALACTOSYL DIACYL GLYCEROL (DGDG)

+
Macromolecule #23: 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE

MacromoleculeName: 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE / type: ligand / ID: 23 / Number of copies: 2 / Formula: LMG
Molecular weightTheoretical: 787.158 Da
Chemical component information

ChemComp-LMG:
1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE

+
Macromolecule #24: ARACHIDONIC ACID

MacromoleculeName: ARACHIDONIC ACID / type: ligand / ID: 24 / Number of copies: 1 / Formula: ACD
Molecular weightTheoretical: 304.467 Da
Chemical component information

ChemComp-ACD:
ARACHIDONIC ACID

+
Macromolecule #25: 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL

MacromoleculeName: 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL
type: ligand / ID: 25 / Number of copies: 1 / Formula: SQD
Molecular weightTheoretical: 795.116 Da
Chemical component information

ChemComp-SQD:
1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration1.2 mg/mL
BufferpH: 7.4
GridModel: Quantifoil R1.2/1.3 / Material: GOLD / Mesh: 400 / Support film - Material: CARBON / Support film - topology: CONTINUOUS / Support film - Film thickness: 2 / Pretreatment - Type: GLOW DISCHARGE
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K2 QUANTUM (4k x 4k) / Detector mode: SUPER-RESOLUTION / Average electron dose: 50.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: DIFFRACTION / Nominal defocus max: 2.0 µm / Nominal defocus min: 1.2 µm
Sample stageCooling holder cryogen: NITROGEN
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Startup modelType of model: INSILICO MODEL
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.2 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 127613
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD

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Atomic model buiding 1

RefinementSpace: REAL / Protocol: BACKBONE TRACE
Output model

PDB-8xks:
The cryo-EM structure of Orf2971-FtsHi motor complex

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