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Structure paper

TitleArchitecture of the ATP-driven motor for protein import into chloroplasts.
Journal, issue, pagesMol Plant, Vol. 17, Issue 11, Page 1702-1718, Year 2024
Publish dateNov 4, 2024
AuthorsNing Wang / Jiale Xing / Xiaodong Su / Junting Pan / Hui Chen / Lifang Shi / Long Si / Wenqiang Yang / Mei Li /
PubMed AbstractThousands of nuclear-encoded proteins are transported into chloroplasts through the TOC-TIC translocon that spans the chloroplast envelope membranes. A motor complex pulls the translocated proteins ...Thousands of nuclear-encoded proteins are transported into chloroplasts through the TOC-TIC translocon that spans the chloroplast envelope membranes. A motor complex pulls the translocated proteins out of the TOC-TIC complex into the chloroplast stroma by hydrolyzing ATP. The Orf2971-FtsHi complex has been suggested to serve as the ATP-hydrolyzing motor in Chlamydomonas reinhardtii, but little is known about its architecture and assembly. Here, we report the 3.2-Å resolution structure of the Chlamydomonas Orf2971-FtsHi complex. The 20-subunit complex spans the chloroplast inner envelope, with two bulky modules protruding into the intermembrane space and stromal matrix. Six subunits form a hetero-hexamer that potentially provides the pulling force through ATP hydrolysis. The remaining subunits, including potential enzymes/chaperones, likely facilitate the complex assembly and regulate its proper function. Taken together, our results provide the structural foundation for a mechanistic understanding of chloroplast protein translocation.
External linksMol Plant / PubMed:39327731
MethodsEM (single particle)
Resolution3.2 - 3.4 Å
Structure data

38424

8xks

EMDB-38424, PDB-8xks:
The cryo-EM structure of Orf2971-FtsHi motor complex
Method: EM (single particle) / Resolution: 3.2 Å

EMDB-39187: The masked postprocess map for top region of Orf2971-FtsHi motor complex.
Method: EM (single particle) / Resolution: 3.2 Å

EMDB-39189: The masked postprocess map for bottom region of Orf2971-FtsHi motor complex
Method: EM (single particle) / Resolution: 3.2 Å

EMDB-39201: The Tic22 and Ctap7 within motor complex
Method: EM (single particle) / Resolution: 3.4 Å

EMDB-39202: The middle region of Orf2971-FtsHi motor complex
Method: EM (single particle) / Resolution: 3.3 Å

EMDB-62477: The postprocess Cryo-EM map of Orf2971-Ftshi complex
Method: EM (single particle) / Resolution: 3.3 Å

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-DGA:
DIACYL GLYCEROL

ChemComp-DGD:
DIGALACTOSYL DIACYL GLYCEROL (DGDG)

ChemComp-LMG:
1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE

ChemComp-ACD:
ARACHIDONIC ACID

ChemComp-SQD:
1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL

Source
  • chlamydomonas reinhardtii (plant)
KeywordsPROTEIN TRANSPORT / Chlamydomonas reinhardtii / protein transportation / chloroplast / lipid synthesis / Enzyme

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