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- EMDB-38134: The cryo-EM structure of insect gustatory receptor Gr43a I418A fr... -

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Basic information

Entry
Database: EMDB / ID: EMD-38134
TitleThe cryo-EM structure of insect gustatory receptor Gr43a I418A from Drosophila melanogaster in complex with fructose
Map data
Sample
  • Organelle or cellular component: gustatory receptor Gr43a I418A
    • Protein or peptide: Gustatory receptor for sugar taste 43a
  • Ligand: SODIUM ION
  • Ligand: beta-D-fructofuranose
  • Ligand: water
Keywordsgustatory receptor / ligand-gated ion channel / Gr43a / fructose / MEMBRANE PROTEIN
Function / homology
Function and homology information


sweet taste receptor activity / ionotropic sweet taste receptor activity / male courtship behavior / regulation of appetite / taste receptor activity / chemosensory behavior / adult feeding behavior / sensory perception of sweet taste / regulation of feeding behavior / cellular response to fructose stimulus ...sweet taste receptor activity / ionotropic sweet taste receptor activity / male courtship behavior / regulation of appetite / taste receptor activity / chemosensory behavior / adult feeding behavior / sensory perception of sweet taste / regulation of feeding behavior / cellular response to fructose stimulus / ligand-gated monoatomic cation channel activity / monoatomic cation transmembrane transport / sensory perception of taste / axon / neuronal cell body / dendrite / signal transduction / membrane / plasma membrane
Similarity search - Function
7TM chemoreceptor / 7tm Chemosensory receptor
Similarity search - Domain/homology
Gustatory receptor for sugar taste 43a
Similarity search - Component
Biological speciesDrosophila melanogaster (fruit fly)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.8 Å
AuthorsMa D / Guo J
Funding support China, 1 items
OrganizationGrant numberCountry
Ministry of Science and Technology (MoST, China)2020YFA0908501 China
CitationJournal: Science / Year: 2024
Title: Structural basis for sugar perception by gustatory receptors.
Authors: Demin Ma / Meiqin Hu / Xiaotong Yang / Qiang Liu / Fan Ye / Weijie Cai / Yong Wang / Ximing Xu / Shenghai Chang / Ruiying Wang / Wei Yang / Sheng Ye / Nannan Su / Minrui Fan / Haoxing Xu / Jiangtao Guo /
Abstract: Insects rely on a family of seven transmembrane proteins called gustatory receptors (GRs) to encode different taste modalities, such as sweet and bitter. We report structures of sweet taste ...Insects rely on a family of seven transmembrane proteins called gustatory receptors (GRs) to encode different taste modalities, such as sweet and bitter. We report structures of sweet taste receptors GR43a and GR64a in the apo and sugar-bound states. Both GRs form tetrameric sugar-gated cation channels composed of one central pore domain (PD) and four peripheral ligand-binding domains (LBDs). Whereas GR43a is specifically activated by the monosaccharide fructose that binds to a narrow pocket in LBDs, disaccharides sucrose and maltose selectively activate GR64a by binding to a larger and flatter pocket in LBDs. Sugar binding to LBDs induces local conformational changes, which are subsequently transferred to the PD to cause channel opening. Our studies reveal a structural basis for sugar recognition and activation of GRs.
History
DepositionNov 27, 2023-
Header (metadata) releaseFeb 7, 2024-
Map releaseFeb 7, 2024-
UpdateMar 6, 2024-
Current statusMar 6, 2024Processing site: PDBc / Status: Released

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Structure visualization

Supplemental images

emd_38134.png

Downloads & links

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Map

FileDownload / File: emd_38134.map.gz / Format: CCP4 / Size: 52.7 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
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AxesZ (Sec.)Y (Row.)X (Col.)
0.93 Å/pix.
x 240 pix.
= 223.2 Å		0.93 Å/pix.
x 240 pix.
= 223.2 Å		0.93 Å/pix.
x 240 pix.
= 223.2 Å

Surface

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Images are generated by Spider.

Voxel sizeX=Y=Z: 0.93 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.0197
Minimum - Maximum-0.11165704 - 0.16526158
Average (Standard dev.)0.000084514075 (±0.0058426894)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions240240240
Spacing240240240
CellA=B=C: 223.2 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #2

Fileemd_38134_half_map_1.map
Projections & Slices
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Half map: #1

Fileemd_38134_half_map_2.map
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Sample components

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Entire : gustatory receptor Gr43a I418A

EntireName: gustatory receptor Gr43a I418A
Components
  • Organelle or cellular component: gustatory receptor Gr43a I418A
    • Protein or peptide: Gustatory receptor for sugar taste 43a
  • Ligand: SODIUM ION
  • Ligand: beta-D-fructofuranose
  • Ligand: water

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Supramolecule #1: gustatory receptor Gr43a I418A

SupramoleculeName: gustatory receptor Gr43a I418A / type: organelle_or_cellular_component / ID: 1 / Parent: 0 / Macromolecule list: #1
Source (natural)Organism: Drosophila melanogaster (fruit fly)

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Macromolecule #1: Gustatory receptor for sugar taste 43a

MacromoleculeName: Gustatory receptor for sugar taste 43a / type: protein_or_peptide / ID: 1 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Drosophila melanogaster (fruit fly)
Molecular weightTheoretical: 49.267312 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MEISQPSIGI FYISKVLALA PYATVRNSKG RVEIGRSWLF TVYSATLTVV MVFLTYRGLL FDANSEIPVR MKSATSKVVT ALDVSVVVM AIVSGVYCGL FSLNDTLELN DRLNKIDNTL NAYNNFRRDR WRALGMAAVS LLAISILVGL DVGTWMRIAQ D MNIAQSDT ...String:
MEISQPSIGI FYISKVLALA PYATVRNSKG RVEIGRSWLF TVYSATLTVV MVFLTYRGLL FDANSEIPVR MKSATSKVVT ALDVSVVVM AIVSGVYCGL FSLNDTLELN DRLNKIDNTL NAYNNFRRDR WRALGMAAVS LLAISILVGL DVGTWMRIAQ D MNIAQSDT ELNVHWYIPF YSLYFILTGL QVNIANTAYG LGRRFGRLNR MLSSSFLAEN NATSAIKPQK VSTVKNVSVN RP AMPSALH ASLTKLNGET LPSEAAAKNK GLLLKSLADS HESLGKCVHL LSNSFGIAVL FILVSCLLHL VATAYFLFLE LLS KRDNGY LWVQMLWICF HFLRLLMVVE PCHLAARESR KTIQIVCEIE RKVHEPILAE AVKKFWQQLL VVDADFSACG LCRV NRTIL TSFASAIATY LVALIQFQRT NGLEGGSSGG WSHPQFEK

UniProtKB: Gustatory receptor for sugar taste 43a

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Macromolecule #2: SODIUM ION

MacromoleculeName: SODIUM ION / type: ligand / ID: 2 / Number of copies: 2
Molecular weightTheoretical: 22.99 Da

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Macromolecule #3: beta-D-fructofuranose

MacromoleculeName: beta-D-fructofuranose / type: ligand / ID: 3 / Number of copies: 4 / Formula: FRU
Molecular weightTheoretical: 180.156 Da
Chemical component information

ChemComp-FRU:
beta-D-fructofuranose

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Macromolecule #4: water

MacromoleculeName: water / type: ligand / ID: 4 / Number of copies: 4 / Formula: HOH
Molecular weightTheoretical: 18.015 Da
Chemical component information

ChemComp-HOH:
WATER

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.4
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 52.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 1.5 µm / Nominal defocus min: 0.8 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Startup modelType of model: NONE
Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.8 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 158079
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD

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