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- PDB-8jma: The cryo-EM structure of insect gustatory receptor Gr43a from Dro... -

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Basic information

Entry
Database: PDB / ID: 8jma
TitleThe cryo-EM structure of insect gustatory receptor Gr43a from Drosophila melanogaster in complex with fructose
ComponentsGustatory receptor for sugar taste 43a
KeywordsMEMBRANE PROTEIN / gustatory receptor / ligand-gated ion channel / Gr43a / fructose
Function / homology
Function and homology information


ionotropic sweet taste receptor activity / regulation of appetite / male courtship behavior / sweet taste receptor activity / regulation of feeding behavior / adult feeding behavior / chemosensory behavior / taste receptor activity / sensory perception of sweet taste / cellular response to fructose stimulus ...ionotropic sweet taste receptor activity / regulation of appetite / male courtship behavior / sweet taste receptor activity / regulation of feeding behavior / adult feeding behavior / chemosensory behavior / taste receptor activity / sensory perception of sweet taste / cellular response to fructose stimulus / ligand-gated monoatomic cation channel activity / monoatomic cation transmembrane transport / sensory perception of taste / axon / neuronal cell body / dendrite / signal transduction / membrane / plasma membrane
Similarity search - Function
7TM chemoreceptor / 7tm Chemosensory receptor
Similarity search - Domain/homology
beta-D-fructofuranose / Gustatory receptor for sugar taste 43a
Similarity search - Component
Biological speciesDrosophila melanogaster (fruit fly)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 2.5 Å
AuthorsMa, D. / Guo, J.
Funding support China, 1items
OrganizationGrant numberCountry
Ministry of Science and Technology (MoST, China)2020YFA0908501 China
CitationJournal: Science / Year: 2024
Title: Structural basis for sugar perception by gustatory receptors.
Authors: Demin Ma / Meiqin Hu / Xiaotong Yang / Qiang Liu / Fan Ye / Weijie Cai / Yong Wang / Ximing Xu / Shenghai Chang / Ruiying Wang / Wei Yang / Sheng Ye / Nannan Su / Minrui Fan / Haoxing Xu / Jiangtao Guo /
Abstract: Insects rely on a family of seven transmembrane proteins called gustatory receptors (GRs) to encode different taste modalities, such as sweet and bitter. We report structures of sweet taste ...Insects rely on a family of seven transmembrane proteins called gustatory receptors (GRs) to encode different taste modalities, such as sweet and bitter. We report structures of sweet taste receptors GR43a and GR64a in the apo and sugar-bound states. Both GRs form tetrameric sugar-gated cation channels composed of one central pore domain (PD) and four peripheral ligand-binding domains (LBDs). Whereas GR43a is specifically activated by the monosaccharide fructose that binds to a narrow pocket in LBDs, disaccharides sucrose and maltose selectively activate GR64a by binding to a larger and flatter pocket in LBDs. Sugar binding to LBDs induces local conformational changes, which are subsequently transferred to the PD to cause channel opening. Our studies reveal a structural basis for sugar recognition and activation of GRs.
History
DepositionJun 4, 2023Deposition site: PDBJ / Processing site: PDBC
Revision 1.0Feb 7, 2024Provider: repository / Type: Initial release
Revision 1.1Feb 14, 2024Group: Database references / Category: citation / citation_author
Item: _citation.page_first / _citation.page_last ..._citation.page_first / _citation.page_last / _citation.pdbx_database_id_PubMed / _citation.title / _citation_author.identifier_ORCID
Revision 1.2Mar 6, 2024Group: Database references / Category: citation / Item: _citation.journal_volume

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: Gustatory receptor for sugar taste 43a
B: Gustatory receptor for sugar taste 43a
C: Gustatory receptor for sugar taste 43a
D: Gustatory receptor for sugar taste 43a
hetero molecules


Theoretical massNumber of molelcules
Total (without water)197,9588
Polymers197,2384
Non-polymers7214
Water00
1


  • Idetical with deposited unit
  • defined by author
  • Evidence: electron microscopy, not applicable
TypeNameSymmetry operationNumber
identity operation1_5551

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Components

#1: Protein
Gustatory receptor for sugar taste 43a


Mass: 49309.391 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Drosophila melanogaster (fruit fly) / Gene: Gr43a, GR43.1, CG1712 / Production host: Homo sapiens (human) / References: UniProt: Q9V4K2
#2: Sugar
ChemComp-FRU / beta-D-fructofuranose / beta-D-fructose / D-fructose / fructose


Type: D-saccharide, beta linking / Mass: 180.156 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C6H12O6 / Feature type: SUBJECT OF INVESTIGATION
IdentifierTypeProgram
DFrufbCONDENSED IUPAC CARBOHYDRATE SYMBOLGMML 1.0
b-D-fructofuranoseCOMMON NAMEGMML 1.0
b-D-FrufIUPAC CARBOHYDRATE SYMBOLPDB-CARE 1.0
FruSNFG CARBOHYDRATE SYMBOLGMML 1.0
Has ligand of interestY

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: gustatory receptor Gr43a / Type: ORGANELLE OR CELLULAR COMPONENT / Entity ID: #1 / Source: MULTIPLE SOURCES
Source (natural)Organism: Drosophila melanogaster (fruit fly)
Source (recombinant)Organism: Homo sapiens (human)
Buffer solutionpH: 7.4
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
VitrificationCryogen name: ETHANE

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD / Nominal defocus max: 1500 nm / Nominal defocus min: 800 nm
Image recordingElectron dose: 52 e/Å2 / Film or detector model: FEI FALCON IV (4k x 4k)

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Processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
3D reconstructionResolution: 2.5 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 240297 / Symmetry type: POINT

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