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- EMDB-3812: Tomogram of e. coli carrying the ple7 plasmid carrying YFP-MreB h... -

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Basic information

Database: EMDB / ID: 3812
TitleTomogram of e. coli carrying the ple7 plasmid carrying YFP-MreB hyper-overexpressed by induction with 1uM IPTG
SampleEscherichia Coli
SourceEscherichia coli / bacteria / エシェリキア・コリ, 大腸菌 /
Map data
Methodelectron tomography
AuthorsSwulius MT / Jensen GJ
CitationJ. Bacteriol., 2012, 194, 6382-6386

J. Bacteriol., 2012, 194, 6382-6386 Yorodumi Papers
The helical MreB cytoskeleton in Escherichia coli MC1000/pLE7 is an artifact of the N-Terminal yellow fluorescent protein tag.
Matthew T Swulius / Grant J Jensen

DateDeposition: Jul 14, 2017 / Header (metadata) release: Aug 30, 2017 / Map release: Aug 30, 2017 / Last update: Aug 30, 2017

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Fileemd_3812.map.gz (map file in CCP4 format, 1032257 KB)
Voxel sizeX=Y=Z: 9.46 Å
Contour Level:27.7 (by author)
Minimum - Maximum-128 - 127
Average (Standard dev.)29.99577 (2.205534)


Space Group Number1
Map Geometry
Axis orderXYZ
CellA: 9611.36 Å / B: 9611.36 Å / C: 9460 Å
α=β=γ: 90 deg.

CCP4 map header:

modeenvelope stored as signed bytes (from -128 lowest to 127 highest)
Å/pix. X/Y/Z9.469.469.46
M x/y/z101610161000
origin x/y/z0.0000.0000.000
length x/y/z9611.3609611.3609460.000
start NX/NY/NZ
MAP C/R/S123
start NC/NR/NS000
D min/max/mean-128.000127.00029.996

Supplemental data

Sample components

Entire Escherichia Coli

EntireName: Escherichia Coli / Number of components: 2

Component #1: cellular-component, Escherichia Coli

Cellular-componentName: Escherichia Coli / Recombinant expression: No
SourceSpecies: Escherichia coli / bacteria / エシェリキア・コリ, 大腸菌 /

Component #2: cellular-component, Helical MreB Cytoskeleton

Cellular-componentName: Helical MreB Cytoskeleton / Recombinant expression: No
SourceSpecies: Escherichia coli / bacteria / エシェリキア・コリ, 大腸菌 /

Experimental details

Sample preparation

Specimen statecell
Sample solutionBuffer solution: MC1000, MC1000/pLE6, and MC1000/pLE7 were grown in LB at 37C with 50ug/ml ampicillin when appropriate.
pH: 7
VitrificationCryogen name: NONE

Electron microscopy imaging

Experimental equipment
Model: Tecnai Polara / Image courtesy: FEI Company
ImagingMicroscope: FEI POLARA 300
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Electron dose: 0.4 e/Å2 / Illumination mode: FLOOD BEAM
LensImaging mode: BRIGHT FIELD / Energy filter: FEI
Specimen HolderModel: OTHER / Tilt Angle: -64 - 64 deg. / Temperature: K ( - 123.15 K)
CameraDetector: GATAN ULTRASCAN 4000 (4k x 4k)

Image processing

ProcessingMethod: electron tomography / Tilt angle increment: 1 deg. / Number of sections: 501
3D reconstructionSoftware: IMOD

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