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Yorodumi- EMDB-37749: In situ PBS-PSII supercomplex from cyanobacterial Spirulina platensis -
+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-37749 | |||||||||
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Title | In situ PBS-PSII supercomplex from cyanobacterial Spirulina platensis | |||||||||
Map data | ||||||||||
Sample |
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Keywords | complex / PHOTOSYNTHESIS | |||||||||
Function / homology | Function and homology information phycobilisome / plasma membrane-derived thylakoid membrane / photosynthesis Similarity search - Function | |||||||||
Biological species | Arthrospira platensis (bacteria) / Arthrospira sp. FACHB-439 (bacteria) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.5 Å | |||||||||
Authors | You X / Zhang X / Xiao YN / Sun S / Sui SF | |||||||||
Funding support | 1 items
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Citation | Journal: To Be Published Title: Structure of in situ PBS-PSII supercomplex at 3.5 Angstroms resolution. Authors: You X / Zhang X / Xiao YN / Sun S / Sui SF | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_37749.map.gz | 228.5 MB | EMDB map data format | |
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Header (meta data) | emd-37749-v30.xml emd-37749.xml | 52.4 KB 52.4 KB | Display Display | EMDB header |
Images | emd_37749.png | 123.6 KB | ||
Filedesc metadata | emd-37749.cif.gz | 10.9 KB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-37749 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-37749 | HTTPS FTP |
-Validation report
Summary document | emd_37749_validation.pdf.gz | 567.8 KB | Display | EMDB validaton report |
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Full document | emd_37749_full_validation.pdf.gz | 567.4 KB | Display | |
Data in XML | emd_37749_validation.xml.gz | 7.3 KB | Display | |
Data in CIF | emd_37749_validation.cif.gz | 8.3 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-37749 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-37749 | HTTPS FTP |
-Related structure data
Related structure data | 8wqlMC M: atomic model generated by this map C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_37749.map.gz / Format: CCP4 / Size: 244.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.632 Å | ||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Sample components
+Entire : In situ PBS-PSII supercomplex
+Supramolecule #1: In situ PBS-PSII supercomplex
+Macromolecule #1: LRC1
+Macromolecule #2: LCM
+Macromolecule #3: Allophycocyanin alpha chain
+Macromolecule #4: Allophycocyanin beta chain
+Macromolecule #5: Phycobilisome 7.8 kDa linker polypeptide, allophycocyanin-associa...
+Macromolecule #6: Photosystem II protein D1
+Macromolecule #7: Photosystem II CP47 reaction center protein
+Macromolecule #8: Photosystem II CP43 reaction center protein
+Macromolecule #9: Photosystem II D2 protein
+Macromolecule #10: Cytochrome b559 subunit alpha
+Macromolecule #11: Cytochrome b559 subunit beta
+Macromolecule #12: Photosystem II reaction center protein H
+Macromolecule #13: Photosystem II reaction center protein I
+Macromolecule #14: Photosystem II reaction center protein J
+Macromolecule #15: Photosystem II reaction center protein K
+Macromolecule #16: Photosystem II reaction center protein L
+Macromolecule #17: Photosystem II reaction center protein M
+Macromolecule #18: Photosystem II extrinsic protein O
+Macromolecule #19: PsbQ protein
+Macromolecule #20: Photosystem II reaction center protein Y
+Macromolecule #21: LPP1
+Macromolecule #22: Photosystem II reaction center protein T
+Macromolecule #23: PsbU
+Macromolecule #24: Photosystem II extrinsic protein V
+Macromolecule #25: Photosystem II reaction center protein X
+Macromolecule #26: Photosystem II reaction center protein Psb30
+Macromolecule #27: Photosystem II reaction center protein Z
+Macromolecule #28: LPP2
+Macromolecule #29: Allophycocyanin beta-18 subunit
+Macromolecule #30: Allophycocyanin-B alpha subunit
+Macromolecule #31: C-phycocyanin alpha subunit
+Macromolecule #32: Phycocyanin beta subunit
+Macromolecule #33: PHYCOCYANOBILIN
+Macromolecule #34: CA-MN4-O5 CLUSTER
+Macromolecule #35: CHLORIDE ION
+Macromolecule #36: CHLOROPHYLL A
+Macromolecule #37: 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,...
+Macromolecule #38: 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL
+Macromolecule #39: 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE
+Macromolecule #40: DODECYL-BETA-D-MALTOSIDE
+Macromolecule #41: BICARBONATE ION
+Macromolecule #42: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE
+Macromolecule #43: BETA-CAROTENE
+Macromolecule #44: DIGALACTOSYL DIACYL GLYCEROL (DGDG)
+Macromolecule #45: PHEOPHYTIN A
+Macromolecule #46: FE (II) ION
+Macromolecule #47: PROTOPORPHYRIN IX CONTAINING FE
+Macromolecule #48: CALCIUM ION
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | cell |
-Sample preparation
Buffer | pH: 7.6 |
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Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 35.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Calibrated defocus min: 6.0 µm / Illumination mode: SPOT SCAN / Imaging mode: BRIGHT FIELD / Nominal defocus max: 6.0 µm / Nominal defocus min: 1.0 µm |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
-Image processing
Startup model | Type of model: NONE |
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Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 3.5 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 168000 |
Initial angle assignment | Type: MAXIMUM LIKELIHOOD |
Final angle assignment | Type: MAXIMUM LIKELIHOOD |