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Open data
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Basic information
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Title | Molecular mechanism of prostaglandin transporter SLCO2A1 | |||||||||
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![]() | SLCO family / MEMBRANE PROTEIN | |||||||||
Function / homology | ![]() Defective SLCO2A1 causes primary, autosomal recessive hypertrophic osteoarthropathy 2 (PHOAR2) / Transport of organic anions / sodium-independent organic anion transmembrane transporter activity / sodium-independent organic anion transport / prostaglandin transport / prostaglandin transmembrane transporter activity / lipid transporter activity / lipid transport / basal plasma membrane / basolateral plasma membrane ...Defective SLCO2A1 causes primary, autosomal recessive hypertrophic osteoarthropathy 2 (PHOAR2) / Transport of organic anions / sodium-independent organic anion transmembrane transporter activity / sodium-independent organic anion transport / prostaglandin transport / prostaglandin transmembrane transporter activity / lipid transporter activity / lipid transport / basal plasma membrane / basolateral plasma membrane / lysosome / membrane / plasma membrane Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.2 Å | |||||||||
![]() | Li YH / Qu QH / Zhu ZN / Chao YL / Zhou ZX | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Molecular mechanism of prostaglandin transporter SLCO2A1 Authors: Li YH / Qu QH | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 38.2 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 13.1 KB 13.1 KB | Display Display | ![]() |
Images | ![]() | 73.7 KB | ||
Filedesc metadata | ![]() | 5.5 KB | ||
Others | ![]() ![]() | 37.7 MB 37.7 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8kgv M: atomic model generated by this map C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
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Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 0.932 Å | ||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: #2
File | emd_37233_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #1
File | emd_37233_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
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Sample components
-Entire : Membrane Protein sample
Entire | Name: Membrane Protein sample |
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Components |
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-Supramolecule #1: Membrane Protein sample
Supramolecule | Name: Membrane Protein sample / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1 |
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Source (natural) | Organism: ![]() |
-Macromolecule #1: Solute carrier organic anion transporter family member 2A1
Macromolecule | Name: Solute carrier organic anion transporter family member 2A1 type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 68.543078 KDa |
Recombinant expression | Organism: Mammalian expression vector Flag-EGFP-MCS-pcDNA3.1 (others) |
Sequence | String: MGLLPKLGAS QGSDTSTSRA GRCARSVFGN IKVFVLCQGL LQLCQLLYSA YFKSSLTTIE KRFGLSSSSS GLISSLNEIS NAILIIFVS YFGSRVHRPR LIGIGGLFLA AGAFILTLPH FLSEPYQYTL ASTGNNSRLQ AELCQKHWQD LPPSKCHSTT Q NPQKETSS ...String: MGLLPKLGAS QGSDTSTSRA GRCARSVFGN IKVFVLCQGL LQLCQLLYSA YFKSSLTTIE KRFGLSSSSS GLISSLNEIS NAILIIFVS YFGSRVHRPR LIGIGGLFLA AGAFILTLPH FLSEPYQYTL ASTGNNSRLQ AELCQKHWQD LPPSKCHSTT Q NPQKETSS MWGLMVVAQL LAGIGTVPIQ PFGISYVDDF SEPSNSPLYI SILFAISVFG PAFGYLLGSV MLQIFVDYGR VN TAAVNLV PGDPRWIGAW WLGLLISSAL LVLTSFPFFF FPRAMPIGAK RAPATADEAR KLEEAKSRGS LVDFIKRFPC IFL RLLMNS LFVLVVLAQC TFSSVIAGLS TFLNKFLEKQ YGTSAAYANF LIGAVNLPAA ALGMLFGGIL MKRFVFSLQA IPRI ATTII TISMILCVPL FFMGCSTPTV AEVYPPSTSS SIHPQSPACR RDCSCPDSIF HPVCGDNGIE YLSPCHAGCS NINMS SATS KQLIYLNCSC VTGGSASAKT GSCPVPCAHF LLPAIFLISF VSLIACISHN PLYMMVLRVV NQEEKSFAIG VQFLLM RLL AWLPSPALYG LTIDHSCIRW NSLCLGRRGA CAYYDNDALR DRYLGLQMGY KALGMLLLCF ISWRVK UniProtKB: Solute carrier organic anion transporter family member 2A1 |
-Macromolecule #2: 2-acetamido-2-deoxy-beta-D-glucopyranose
Macromolecule | Name: 2-acetamido-2-deoxy-beta-D-glucopyranose / type: ligand / ID: 2 / Number of copies: 1 / Formula: NAG |
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Molecular weight | Theoretical: 221.208 Da |
Chemical component information | ![]() ChemComp-NAG: |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 7.3 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 50.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 2.0 µm / Nominal defocus min: 1.5 µm |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
Startup model | Type of model: NONE |
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Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 3.2 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 238917 |
Initial angle assignment | Type: ANGULAR RECONSTITUTION |
Final angle assignment | Type: ANGULAR RECONSTITUTION |