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基本情報
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タイトル | Cryo-EM structure of an intermediate-state complex during the process of photosystem II repair | ||||||||||||
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![]() | monomeric Photosystem II / PSII repair / PHOTOSYNTHESIS | ||||||||||||
機能・相同性 | ![]() photosystem II repair / oxygen evolving activity / photosystem II stabilization / photosystem II reaction center / photosystem II / oxidoreductase activity, acting on diphenols and related substances as donors, oxygen as acceptor / photosynthetic electron transport chain / response to herbicide / photosystem II / photosynthetic electron transport in photosystem II ...photosystem II repair / oxygen evolving activity / photosystem II stabilization / photosystem II reaction center / photosystem II / oxidoreductase activity, acting on diphenols and related substances as donors, oxygen as acceptor / photosynthetic electron transport chain / response to herbicide / photosystem II / photosynthetic electron transport in photosystem II / chlorophyll binding / phosphate ion binding / photosynthesis, light reaction / chloroplast thylakoid membrane / : / photosynthesis / electron transfer activity / protein stabilization / iron ion binding / heme binding / metal ion binding / membrane 類似検索 - 分子機能 | ||||||||||||
生物種 | ![]() ![]() | ||||||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 2.6 Å | ||||||||||||
![]() | Li A / Wang Y / Liu Z | ||||||||||||
資金援助 | ![]()
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![]() | ![]() タイトル: Structural basis for an early stage of the photosystem II repair cycle in Chlamydomonas reinhardtii. 著者: Anjie Li / Tingting You / Xiaojie Pang / Yidi Wang / Lijin Tian / Xiaobo Li / Zhenfeng Liu / ![]() 要旨: Photosystem II (PSII) catalyzes water oxidation and plastoquinone reduction by utilizing light energy. It is highly susceptible to photodamage under high-light conditions and the damaged PSII needs ...Photosystem II (PSII) catalyzes water oxidation and plastoquinone reduction by utilizing light energy. It is highly susceptible to photodamage under high-light conditions and the damaged PSII needs to be restored through a process known as the PSII repair cycle. The detailed molecular mechanism underlying the PSII repair process remains mostly elusive. Here, we report biochemical and structural features of a PSII-repair intermediate complex, likely arrested at an early stage of the PSII repair process in the green alga Chlamydomonas reinhardtii. The complex contains three protein factors associated with a damaged PSII core, namely Thylakoid Enriched Factor 14 (TEF14), Photosystem II Repair Factor 1 (PRF1), and Photosystem II Repair Factor 2 (PRF2). TEF14, PRF1 and PRF2 may facilitate the release of the manganese-stabilizing protein PsbO, disassembly of peripheral light-harvesting complexes from PSII and blockage of the Q site, respectively. Moreover, an α-tocopherol quinone molecule is located adjacent to the heme group of cytochrome b, potentially fulfilling a photoprotective role by preventing the generation of reactive oxygen species. | ||||||||||||
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画像 | ![]() | 142.7 KB | ||
Filedesc metadata | ![]() | 8.4 KB | ||
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-検証レポート
文書・要旨 | ![]() | 476.9 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 476.5 KB | 表示 | |
XML形式データ | ![]() | 12.2 KB | 表示 | |
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-関連構造データ
関連構造データ | ![]() 8kdeMC ![]() 8zeeC C: 同じ文献を引用 ( M: このマップから作成された原子モデル |
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類似構造データ | 類似検索 - 機能・相同性 ![]() |
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「今月の分子」の関連する項目 |
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マップ
ファイル | ![]() | ||||||||||||||||||||||||||||||||||||
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投影像・断面図 | 画像のコントロール
画像は Spider により作成 | ||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 1.04 Å | ||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
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-添付データ
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試料の構成要素
+全体 : Cryo-EM structure of an intermediate-state complex during the pro...
+超分子 #1: Cryo-EM structure of an intermediate-state complex during the pro...
+分子 #1: Photosystem II CP47 reaction center protein
+分子 #2: Photosystem II D2 protein
+分子 #3: Cytochrome b559 subunit alpha
+分子 #4: Cytochrome b559 subunit beta
+分子 #5: Photosystem II reaction center protein H
+分子 #6: Photosystem II reaction center protein I
+分子 #7: Photosystem II reaction center protein K
+分子 #8: Photosystem II reaction center protein L
+分子 #9: Photosystem II reaction center protein M
+分子 #10: Photosystem II reaction center protein T
+分子 #11: Photosystem II reaction center protein Ycf12
+分子 #12: Uncharacterized protein
+分子 #13: Photosystem II reaction center protein Z
+分子 #14: Thylakoid enriched factor 14 (TEF14)
+分子 #15: Photosystem II repair factor 1 (PRF1)
+分子 #16: Photosystem II CP43 reaction center protein
+分子 #17: Photosystem II protein D1
+分子 #18: Photosystem II repair factor 2 (PRF2)
+分子 #19: CHLOROPHYLL A
+分子 #20: BETA-CAROTENE
+分子 #21: 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE
+分子 #22: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE
+分子 #23: 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,...
+分子 #24: PHEOPHYTIN A
+分子 #25: PROTOPORPHYRIN IX CONTAINING FE
+分子 #26: RRR-ALPHA-TOCOPHERYLQUINONE
+分子 #27: DIGALACTOSYL DIACYL GLYCEROL (DGDG)
+分子 #28: 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL
+分子 #29: BICARBONATE ION
+分子 #30: FE (II) ION
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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![]() | 単粒子再構成法 |
試料の集合状態 | particle |
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試料調製
緩衝液 | pH: 6.5 |
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凍結 | 凍結剤: ETHANE |
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電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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撮影 | フィルム・検出器のモデル: GATAN K2 SUMMIT (4k x 4k) 平均電子線量: 60.0 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: ![]() |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / 最大 デフォーカス(公称値): 1.5 µm / 最小 デフォーカス(公称値): 1.0 µm |
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |