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- EMDB-36907: Cryo-EM structure of the RC-LH core comples from Halorhodospira h... -

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Basic information

Entry
Database: EMDB / ID: EMD-36907
TitleCryo-EM structure of the RC-LH core comples from Halorhodospira halochloris
Map data
Sample
  • Complex: RC-LH core complex
    • Protein or peptide: x 10 types
  • Protein or peptide: x 1 types
  • Ligand: x 14 types
KeywordsRC-LH complex / HALORHODOSPIRA HALOCHLORIS / PHOTOSYNTHESIS
Function / homology
Function and homology information


organelle inner membrane / plasma membrane-derived chromatophore membrane / plasma membrane light-harvesting complex / bacteriochlorophyll binding / photosynthesis, light reaction / electron transporter, transferring electrons within the cyclic electron transport pathway of photosynthesis activity / photosynthetic electron transport in photosystem II / electron transfer activity / iron ion binding / heme binding ...organelle inner membrane / plasma membrane-derived chromatophore membrane / plasma membrane light-harvesting complex / bacteriochlorophyll binding / photosynthesis, light reaction / electron transporter, transferring electrons within the cyclic electron transport pathway of photosynthesis activity / photosynthetic electron transport in photosystem II / electron transfer activity / iron ion binding / heme binding / membrane / metal ion binding / plasma membrane
Similarity search - Function
Photosynthetic reaction centre, cytochrome c subunit / Multihaem cytochrome, PRC subunit superfamily / Photosynthetic reaction centre cytochrome C subunit / Antenna complex, beta subunit, conserved site / Antenna complexes beta subunits signature. / Antenna complex, alpha subunit / Antenna complex, beta domain superfamily / Antenna complex, alpha subunit conserved site / Antenna complexes alpha subunits signature. / Light-harvesting protein B beta chain ...Photosynthetic reaction centre, cytochrome c subunit / Multihaem cytochrome, PRC subunit superfamily / Photosynthetic reaction centre cytochrome C subunit / Antenna complex, beta subunit, conserved site / Antenna complexes beta subunits signature. / Antenna complex, alpha subunit / Antenna complex, beta domain superfamily / Antenna complex, alpha subunit conserved site / Antenna complexes alpha subunits signature. / Light-harvesting protein B beta chain / Antenna complex, alpha/beta subunit / Light-harvesting complex / Antenna complex alpha/beta subunit / Photosynthetic reaction centre, H subunit / Bacterial photosynthetic reaction centre, H-chain, C-terminal / Photosynthetic reaction centre, M subunit / Photosynthetic reaction centre, H subunit, N-terminal / Photosynthetic reaction centre, H subunit, N-terminal domain superfamily / Photosynthetic reaction centre, H-chain N-terminal region / PRC-barrel domain / PRC-barrel domain / Photosynthetic reaction centre, L subunit / PRC-barrel-like superfamily / Multiheme cytochrome superfamily / Photosynthetic reaction centre, L/M / Photosystem II protein D1/D2 superfamily / Photosynthetic reaction centre protein / Photosynthetic reaction center proteins signature. / Prokaryotic membrane lipoprotein lipid attachment site profile.
Similarity search - Domain/homology
Photosynthetic reaction center cytochrome c subunit / Photosynthetic reaction center H subunit / Reaction center protein M chain / Uncharacterized protein / Reaction center protein L chain / Antenna complex alpha/beta subunit domain-containing protein / Antenna complex alpha/beta subunit domain-containing protein / Light-harvesting LHI
Similarity search - Component
Biological speciesHalorhodospira halochloris (bacteria)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.42 Å
AuthorsWang G-L / Qi C-H / Yu L-J
Funding support China, 1 items
OrganizationGrant numberCountry
Ministry of Science and Technology (MoST, China)2022YFC3401800 China
CitationJournal: J Integr Plant Biol / Year: 2024
Title: Structural insights into the unusual core photocomplex from a triply extremophilic purple bacterium, Halorhodospira halochloris.
Authors: Chen-Hui Qi / Guang-Lei Wang / Fang-Fang Wang / Jie Wang / Xiang-Ping Wang / Mei-Juan Zou / Fei Ma / Michael T Madigan / Yukihiro Kimura / Zheng-Yu Wang-Otomo / Long-Jiang Yu /
Abstract: Halorhodospira (Hlr.) halochloris is a triply extremophilic phototrophic purple sulfur bacterium, as it is thermophilic, alkaliphilic, and extremely halophilic. The light-harvesting-reaction center ...Halorhodospira (Hlr.) halochloris is a triply extremophilic phototrophic purple sulfur bacterium, as it is thermophilic, alkaliphilic, and extremely halophilic. The light-harvesting-reaction center (LH1-RC) core complex of this bacterium displays an LH1-Q transition at 1,016 nm, which is the lowest-energy wavelength absorption among all known phototrophs. Here we report the cryo-EM structure of the LH1-RC at 2.42 Å resolution. The LH1 complex forms a tricyclic ring structure composed of 16 αβγ-polypeptides and one αβ-heterodimer around the RC. From the cryo-EM density map, two previously unrecognized integral membrane proteins, referred to as protein G and protein Q, were identified. Both of these proteins are single transmembrane-spanning helices located between the LH1 ring and the RC L-subunit and are absent from the LH1-RC complexes of all other purple bacteria of which the structures have been determined so far. Besides bacteriochlorophyll b molecules (B1020) located on the periplasmic side of the Hlr. halochloris membrane, there are also two arrays of bacteriochlorophyll b molecules (B800 and B820) located on the cytoplasmic side. Only a single copy of a carotenoid (lycopene) was resolved in the Hlr. halochloris LH1-α3β3 and this was positioned within the complex. The potential quinone channel should be the space between the LH1-α3β3 that accommodates the single lycopene but does not contain a γ-polypeptide, B800 and B820. Our results provide a structural explanation for the unusual Q red shift and carotenoid absorption in the Hlr. halochloris spectrum and reveal new insights into photosynthetic mechanisms employed by a species that thrives under the harshest conditions of any phototrophic microorganism known.
History
DepositionJul 22, 2023-
Header (metadata) releaseMay 22, 2024-
Map releaseMay 22, 2024-
UpdateMay 22, 2024-
Current statusMay 22, 2024Processing site: PDBj / Status: Released

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Structure visualization

Supplemental images

emd_36907.png

Downloads & links

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Map

FileDownload / File: emd_36907.map.gz / Format: CCP4 / Size: 178 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Voxel sizeX=Y=Z: 1.02 Å
Density
Contour LevelBy AUTHOR: 0.4
Minimum - Maximum-1.6558168 - 2.8824334
Average (Standard dev.)0.00016962357 (±0.07369802)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions360360360
Spacing360360360
CellA=B=C: 367.19998 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #2

Fileemd_36907_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_36907_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : RC-LH core complex

EntireName: RC-LH core complex
Components
  • Complex: RC-LH core complex
    • Protein or peptide: Photosynthetic reaction center cytochrome c subunit
    • Protein or peptide: Reaction center protein M chainPhotosynthetic reaction centre
    • Protein or peptide: Photosynthetic reaction center H subunit
    • Protein or peptide: Antenna complex alpha/beta subunit domain-containing protein
    • Protein or peptide: Light-harvesting LHI
    • Protein or peptide: Antenna complex alpha/beta subunit domain-containing protein
    • Protein or peptide: Beta subunit of light-harvesting 1
    • Protein or peptide: Gamma subunit of light-harvesting 1
    • Protein or peptide: reaction center small polypeptide
    • Protein or peptide: reactin center small polypeptide
  • Protein or peptide: Reaction center protein L chainPhotosynthetic reaction centre
  • Ligand: HEME C
  • Ligand: (1R)-2-{[{[(2S)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL (11E)-OCTADEC-11-ENOATE
  • Ligand: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE
  • Ligand: Trans-Geranyl Bacteriochlorophyll B
  • Ligand: Trans-Geranyl Bacteriopheophytin B
  • Ligand: Ubiquinone-8Coenzyme Q10
  • Ligand: UNKNOWN LIGAND
  • Ligand: FE (III) ION
  • Ligand: MENAQUINONE 8Vitamin K2
  • Ligand: CARDIOLIPIN
  • Ligand: LYCOPENE
  • Ligand: Trans-Geranyl 8-vinyl-bacteriochlorophyll B
  • Ligand: DI-PALMITOYL-3-SN-PHOSPHATIDYLETHANOLAMINE
  • Ligand: 2-O-octyl-beta-D-glucopyranose

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Supramolecule #1: RC-LH core complex

SupramoleculeName: RC-LH core complex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1, #3-#11
Source (natural)Organism: Halorhodospira halochloris (bacteria)

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Macromolecule #1: Photosynthetic reaction center cytochrome c subunit

MacromoleculeName: Photosynthetic reaction center cytochrome c subunit / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Halorhodospira halochloris (bacteria)
Molecular weightTheoretical: 41.612859 KDa
SequenceString: MDGRYLTRSL AVGALAAGFL VVAGCERPPF EQEQTGPRGT GMYVLDNPRI LESRLDLHTA PEARPMASED GERAGDVHEN VQVLADLSD EQFWRIKEEM TDWVAGDEGC TYCHTDDLAS DEKYQYRVSR DMIEMTRYLN ANWADTHLTH SNEAGVTCYT C HRGEPIPP ...String:
MDGRYLTRSL AVGALAAGFL VVAGCERPPF EQEQTGPRGT GMYVLDNPRI LESRLDLHTA PEARPMASED GERAGDVHEN VQVLADLSD EQFWRIKEEM TDWVAGDEGC TYCHTDDLAS DEKYQYRVSR DMIEMTRYLN ANWADTHLTH SNEAGVTCYT C HRGEPIPP ASWHSEEESG ETRFMTGMGD LQLQNKISSK TAYTAFPRDA LDTFLVGHEG ELSIVGEGEG GLRTATTEGV SL REAYEAV GLMMHLSYSL DAGCTLCHNV SRWASWEDSP KERETAWHGI RMARDINVNW INPLIDEYPE DADVLGPTGD VGK VSCQTC HNKERRPLYG EEFLELYPEL VGEPDPDFDY LQFGDLGTDL LKGVND

UniProtKB: Photosynthetic reaction center cytochrome c subunit

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Macromolecule #2: Reaction center protein L chain

MacromoleculeName: Reaction center protein L chain / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Halorhodospira halochloris (bacteria)
Molecular weightTheoretical: 31.115025 KDa
SequenceString: MAMLDFERKY RVRGGTLLGG DLFDFWVGPF YVGFFGVTAI FCAVFGFLMI GLKAAISETW SIFQLVLAPP NLENGFALAP LDEGGLWQI VTACAIGAFV SWALREVEIS RKLGIGYHIP FAFGVAISFF VLAQLGRPLL LGGWGHAFPY GIIAHLDWVN N VGYQNLHY ...String:
MAMLDFERKY RVRGGTLLGG DLFDFWVGPF YVGFFGVTAI FCAVFGFLMI GLKAAISETW SIFQLVLAPP NLENGFALAP LDEGGLWQI VTACAIGAFV SWALREVEIS RKLGIGYHIP FAFGVAISFF VLAQLGRPLL LGGWGHAFPY GIIAHLDWVN N VGYQNLHY HYHWAHMLGC SLFFATSFAL ALHGGLILSV TNPKKGEVVK TAEHENTFFR DFVGYSIGSL GIHRLGLALA LS TSISCIF GILTTGPFWS RGWPEWWYTW WPQIPIWNWG VS

UniProtKB: Reaction center protein L chain

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Macromolecule #3: Reaction center protein M chain

MacromoleculeName: Reaction center protein M chain / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Halorhodospira halochloris (bacteria)
Molecular weightTheoretical: 36.221676 KDa
SequenceString: MAEYQNVFTR VQVRGPAEQG LEVPGGSWNR VGRPRFSYLL GKIGDAQVGP IYLGATGVVA SLGFLIFCLM VGFNWLAAVD WSVREVFRQ FWWLAVEVPP PEYGLRIPPF NDGGWFLWGL AICSLSLLMW WARTYIRARA LGLGTHVAWA FAAALWFYFI I TIIRPVAI ...String:
MAEYQNVFTR VQVRGPAEQG LEVPGGSWNR VGRPRFSYLL GKIGDAQVGP IYLGATGVVA SLGFLIFCLM VGFNWLAAVD WSVREVFRQ FWWLAVEVPP PEYGLRIPPF NDGGWFLWGL AICSLSLLMW WARTYIRARA LGLGTHVAWA FAAALWFYFI I TIIRPVAI GSWDESLPIG MFAHLDWLVA ISERYGNFYY NPFHMLSIAF CFGSALLFAA HGATILATGR YNSEREIEQI TD RGTGSER AALFWRWTMG FNATMESIHR WGYWMAILVP LVASIGLFLS GTVIESWYEW GLKHNLVPIY EELSDPARNP AAQ

UniProtKB: Reaction center protein M chain

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Macromolecule #4: Photosynthetic reaction center H subunit

MacromoleculeName: Photosynthetic reaction center H subunit / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Halorhodospira halochloris (bacteria)
Molecular weightTheoretical: 31.293768 KDa
SequenceString: MEAFYPMGIA RFDWGIWAVI FFFVFLAGLI VYCRREDKRE GYPLISDPND KYGAPRLVSG TIPRVPKPKT FLLRDGRTIQ VPRQEKVEW DRNYKLEAQP TAPWPGSPLE PIGNPMKAAI GPGAYAKRED KPELTWHNKQ KIVPMRIATE YYVVEDDPDL R GAPVVGLC ...String:
MEAFYPMGIA RFDWGIWAVI FFFVFLAGLI VYCRREDKRE GYPLISDPND KYGAPRLVSG TIPRVPKPKT FLLRDGRTIQ VPRQEKVEW DRNYKLEAQP TAPWPGSPLE PIGNPMKAAI GPGAYAKRED KPELTWHNKQ KIVPMRIATE YYVVEDDPDL R GAPVVGLC GGQGGRVRDI WVDRSECRIM YYEVEISPHT SGKDSVLLPQ CFARETRRMD GVWEIRVNSI TAEQFRDVPR LS NPDQITP QEEDMVCAYY GAGTLYAVPG RTEPFLP

UniProtKB: Photosynthetic reaction center H subunit

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Macromolecule #5: Antenna complex alpha/beta subunit domain-containing protein

MacromoleculeName: Antenna complex alpha/beta subunit domain-containing protein
type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Halorhodospira halochloris (bacteria)
Molecular weightTheoretical: 12.053332 KDa
SequenceString:
MANDIRPLRD FTDEEAQEFH QAAVQSFFLY VAVAFVAHLL VWAWRPFWPP EQGYRLEDFA PEEIRTDSFY SDFLPTTSWN SVTEGVNSL AAQLSTVVHY IAPFAG

UniProtKB: Antenna complex alpha/beta subunit domain-containing protein

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Macromolecule #6: Light-harvesting LHI

MacromoleculeName: Light-harvesting LHI / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Halorhodospira halochloris (bacteria)
Molecular weightTheoretical: 7.562918 KDa
SequenceString:
MWRIWKVFDP RRILIATALW LIIISLTIHV ILMTTERFNW LQGAPAAEYY SEVVEDGAAL SPRLV

UniProtKB: Light-harvesting LHI

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Macromolecule #7: Antenna complex alpha/beta subunit domain-containing protein

MacromoleculeName: Antenna complex alpha/beta subunit domain-containing protein
type: protein_or_peptide / ID: 7 / Number of copies: 16 / Enantiomer: LEVO
Source (natural)Organism: Halorhodospira halochloris (bacteria)
Molecular weightTheoretical: 7.696815 KDa
SequenceString:
MWKLWKFVDF RMTAVGFHLF FALLAFAVHF ACISSERFNW LEGAPAAEYY MDEDPGIWKR TSYDG

UniProtKB: Antenna complex alpha/beta subunit domain-containing protein

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Macromolecule #8: Beta subunit of light-harvesting 1

MacromoleculeName: Beta subunit of light-harvesting 1 / type: protein_or_peptide / ID: 8 / Number of copies: 16 / Enantiomer: LEVO
Source (natural)Organism: Halorhodospira halochloris (bacteria)
Molecular weightTheoretical: 9.555675 KDa
SequenceString:
MTDIRTGLTD EECQEIHEMN MLGMHAYWSI GLIANALAYA WRPFHQGRAG NRLEDHAPDY VRSALTSVQE QASSVTAAVQ QTPMVG

UniProtKB: Uncharacterized protein

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Macromolecule #9: Gamma subunit of light-harvesting 1

MacromoleculeName: Gamma subunit of light-harvesting 1 / type: protein_or_peptide / ID: 9 / Number of copies: 16 / Enantiomer: LEVO
Source (natural)Organism: Halorhodospira halochloris (bacteria)
Molecular weightTheoretical: 3.123757 KDa
SequenceString:
MGDAGIVVAV LVILAILGWP NISSTLRRW

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Macromolecule #10: reaction center small polypeptide

MacromoleculeName: reaction center small polypeptide / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Halorhodospira halochloris (bacteria)
Molecular weightTheoretical: 3.531281 KDa
SequenceString:
MTGEAVWLIW FMAALALIGG ALPIVVKWWR E

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Macromolecule #11: reactin center small polypeptide

MacromoleculeName: reactin center small polypeptide / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Halorhodospira halochloris (bacteria)
Molecular weightTheoretical: 3.76249 KDa
SequenceString:
MEGHMDVVDI SWLVTLAVLA LLAGAYPVFK RWR

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Macromolecule #12: HEME C

MacromoleculeName: HEME C / type: ligand / ID: 12 / Number of copies: 4 / Formula: HEC
Molecular weightTheoretical: 618.503 Da
Chemical component information

ChemComp-HEC:
HEME C / Heme C

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Macromolecule #13: (1R)-2-{[{[(2S)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-...

MacromoleculeName: (1R)-2-{[{[(2S)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL (11E)-OCTADEC-11-ENOATE
type: ligand / ID: 13 / Number of copies: 50 / Formula: PGV
Molecular weightTheoretical: 749.007 Da
Chemical component information

ChemComp-PGV:
(1R)-2-{[{[(2S)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL (11E)-OCTADEC-11-ENOATE / phospholipid*YM / Phosphatidylglycerol

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Macromolecule #14: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE

MacromoleculeName: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE / type: ligand / ID: 14 / Number of copies: 1 / Formula: LHG
Molecular weightTheoretical: 722.97 Da
Chemical component information

ChemComp-LHG:
1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE / phospholipid*YM / Phosphatidylglycerol

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Macromolecule #15: Trans-Geranyl Bacteriochlorophyll B

MacromoleculeName: Trans-Geranyl Bacteriochlorophyll B / type: ligand / ID: 15 / Number of copies: 54 / Formula: A1LZM
Molecular weightTheoretical: 907.472 Da

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Macromolecule #16: Trans-Geranyl Bacteriopheophytin B

MacromoleculeName: Trans-Geranyl Bacteriopheophytin B / type: ligand / ID: 16 / Number of copies: 2 / Formula: A1LZP
Molecular weightTheoretical: 885.183 Da

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Macromolecule #17: Ubiquinone-8

MacromoleculeName: Ubiquinone-8 / type: ligand / ID: 17 / Number of copies: 1 / Formula: UQ8
Molecular weightTheoretical: 727.109 Da
Chemical component information

ChemComp-UQ8:
Ubiquinone-8

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Macromolecule #18: UNKNOWN LIGAND

MacromoleculeName: UNKNOWN LIGAND / type: ligand / ID: 18 / Number of copies: 57 / Formula: UNL
Molecular weightTheoretical: 909.488 Da
Chemical component information


ChemComp, No image

ChemComp-UNL:
Unknown ligand

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Macromolecule #19: FE (III) ION

MacromoleculeName: FE (III) ION / type: ligand / ID: 19 / Number of copies: 1 / Formula: FE
Molecular weightTheoretical: 55.845 Da

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Macromolecule #20: MENAQUINONE 8

MacromoleculeName: MENAQUINONE 8 / type: ligand / ID: 20 / Number of copies: 2 / Formula: MQ8
Molecular weightTheoretical: 717.116 Da
Chemical component information

ChemComp-MQ8:
MENAQUINONE 8 / Vitamin K2

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Macromolecule #21: CARDIOLIPIN

MacromoleculeName: CARDIOLIPIN / type: ligand / ID: 21 / Number of copies: 3 / Formula: CDL
Molecular weightTheoretical: 1.464043 KDa
Chemical component information

ChemComp-CDL:
CARDIOLIPIN / phospholipid*YM / Cardiolipin

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Macromolecule #22: LYCOPENE

MacromoleculeName: LYCOPENE / type: ligand / ID: 22 / Number of copies: 1 / Formula: LYC
Molecular weightTheoretical: 536.873 Da
Chemical component information

ChemComp-LYC:
LYCOPENE / Lycopene

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Macromolecule #23: Trans-Geranyl 8-vinyl-bacteriochlorophyll B

MacromoleculeName: Trans-Geranyl 8-vinyl-bacteriochlorophyll B / type: ligand / ID: 23 / Number of copies: 16 / Formula: A1LZQ
Molecular weightTheoretical: 907.472 Da

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Macromolecule #24: DI-PALMITOYL-3-SN-PHOSPHATIDYLETHANOLAMINE

MacromoleculeName: DI-PALMITOYL-3-SN-PHOSPHATIDYLETHANOLAMINE / type: ligand / ID: 24 / Number of copies: 1 / Formula: PEF
Molecular weightTheoretical: 691.959 Da
Chemical component information

ChemComp-PEF:
DI-PALMITOYL-3-SN-PHOSPHATIDYLETHANOLAMINE / phospholipid*YM / Phosphatidylethanolamine

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Macromolecule #25: 2-O-octyl-beta-D-glucopyranose

MacromoleculeName: 2-O-octyl-beta-D-glucopyranose / type: ligand / ID: 25 / Number of copies: 2 / Formula: BGL
Molecular weightTheoretical: 292.369 Da
Chemical component information

ChemComp-BGL:
2-O-octyl-beta-D-glucopyranose / detergent*YM

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TALOS ARCTICA
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Nominal defocus max: 2.3000000000000003 µm / Nominal defocus min: 0.7000000000000001 µm
Image recordingFilm or detector model: GATAN K2 BASE (4k x 4k) / Average electron dose: 60.8 e/Å2
Experimental equipment
Model: Talos Arctica / Image courtesy: FEI Company

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Image processing

Startup modelType of model: NONE
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD
Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.42 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 353518
FSC plot (resolution estimation)

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