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- EMDB-36417: The cryo-EM structure of insect gustatory receptor Gr64a from Dro... -

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Basic information

Entry
Database: EMDB / ID: EMD-36417
TitleThe cryo-EM structure of insect gustatory receptor Gr64a from Drosophila melanogaster
Map data
Sample
  • Organelle or cellular component: gustatory receptor Gr64a
    • Protein or peptide: Gustatory receptor for sugar taste 64a
Keywordsgustatory receptor / Gr64a / ligand-gated ion channel / MEMBRANE PROTEIN
Function / homology
Function and homology information


proboscis extension reflex / sweet taste receptor activity / detection of chemical stimulus involved in sensory perception of taste / ionotropic sweet taste receptor activity / taste receptor activity / sensory perception of sweet taste / ligand-gated monoatomic ion channel activity / sensory perception of taste / monoatomic ion transmembrane transport / signal transduction ...proboscis extension reflex / sweet taste receptor activity / detection of chemical stimulus involved in sensory perception of taste / ionotropic sweet taste receptor activity / taste receptor activity / sensory perception of sweet taste / ligand-gated monoatomic ion channel activity / sensory perception of taste / monoatomic ion transmembrane transport / signal transduction / membrane / plasma membrane
Similarity search - Function
Gustatory receptor / Trehalose receptor
Similarity search - Domain/homology
Gustatory receptor for sugar taste 64a
Similarity search - Component
Biological speciesDrosophila melanogaster (fruit fly)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.5 Å
AuthorsMa D / Guo J
Funding support China, 1 items
OrganizationGrant numberCountry
Ministry of Science and Technology (MoST, China)2020YFA0908501 China
CitationJournal: Science / Year: 2024
Title: Structural basis for sugar perception by gustatory receptors.
Authors: Demin Ma / Meiqin Hu / Xiaotong Yang / Qiang Liu / Fan Ye / Weijie Cai / Yong Wang / Ximing Xu / Shenghai Chang / Ruiying Wang / Wei Yang / Sheng Ye / Nannan Su / Minrui Fan / Haoxing Xu / Jiangtao Guo /
Abstract: Insects rely on a family of seven transmembrane proteins called gustatory receptors (GRs) to encode different taste modalities, such as sweet and bitter. We report structures of sweet taste ...Insects rely on a family of seven transmembrane proteins called gustatory receptors (GRs) to encode different taste modalities, such as sweet and bitter. We report structures of sweet taste receptors GR43a and GR64a in the apo and sugar-bound states. Both GRs form tetrameric sugar-gated cation channels composed of one central pore domain (PD) and four peripheral ligand-binding domains (LBDs). Whereas GR43a is specifically activated by the monosaccharide fructose that binds to a narrow pocket in LBDs, disaccharides sucrose and maltose selectively activate GR64a by binding to a larger and flatter pocket in LBDs. Sugar binding to LBDs induces local conformational changes, which are subsequently transferred to the PD to cause channel opening. Our studies reveal a structural basis for sugar recognition and activation of GRs.
History
DepositionJun 4, 2023-
Header (metadata) releaseFeb 7, 2024-
Map releaseFeb 7, 2024-
UpdateMar 6, 2024-
Current statusMar 6, 2024Processing site: PDBc / Status: Released

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Structure visualization

Supplemental images

emd_36417.png

Downloads & links

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Map

FileDownload / File: emd_36417.map.gz / Format: CCP4 / Size: 52.7 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

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AxesZ (Sec.)Y (Row.)X (Col.)
0.93 Å/pix.
x 240 pix.
= 223.2 Å		0.93 Å/pix.
x 240 pix.
= 223.2 Å		0.93 Å/pix.
x 240 pix.
= 223.2 Å

Surface

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Images are generated by Spider.

Voxel sizeX=Y=Z: 0.93 Å
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Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.0228
Minimum - Maximum-0.14460501 - 0.21518269
Average (Standard dev.)0.000003233678 (±0.00731801)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions240240240
Spacing240240240
CellA=B=C: 223.2 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #1

Fileemd_36417_half_map_1.map
Projections & Slices
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Half map: #2

Fileemd_36417_half_map_2.map
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Sample components

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Entire : gustatory receptor Gr64a

EntireName: gustatory receptor Gr64a
Components
  • Organelle or cellular component: gustatory receptor Gr64a
    • Protein or peptide: Gustatory receptor for sugar taste 64a

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Supramolecule #1: gustatory receptor Gr64a

SupramoleculeName: gustatory receptor Gr64a / type: organelle_or_cellular_component / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Drosophila melanogaster (fruit fly)

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Macromolecule #1: Gustatory receptor for sugar taste 64a

MacromoleculeName: Gustatory receptor for sugar taste 64a / type: protein_or_peptide / ID: 1 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Drosophila melanogaster (fruit fly)
Molecular weightTheoretical: 54.66825 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MWSHPQFEKG GSSGGVDMKG PNLNFRKTPS KDNGVKQVES LARPETPPPK FVEDSNLEFN VLASEKLPNY TNLDLFHRAV FPFMFLAQC VAIMPLVGIR ESNPRRVRFA YKSIPMFVTL IFMIATSILF LSMFTHLLKI GITAKNFVGL VFFGCVLSAY V VFIRLAKK ...String:
MWSHPQFEKG GSSGGVDMKG PNLNFRKTPS KDNGVKQVES LARPETPPPK FVEDSNLEFN VLASEKLPNY TNLDLFHRAV FPFMFLAQC VAIMPLVGIR ESNPRRVRFA YKSIPMFVTL IFMIATSILF LSMFTHLLKI GITAKNFVGL VFFGCVLSAY V VFIRLAKK WPAVVRIWTR TEIPFTKPPY EIPKRNLSRR VQLAALAIIG LSLGEHALYQ VSAILSYTRR IQMCANITTV PS FNNYMQT NYDYVFQLLP YSPIIAVLIL LINGACTFVW NYMDLFIMMI SKGLSYRFEQ ITTRIRKLEH EEVCESVFIQ IRE HYVKMC ELLEFVDSAM SSLILLSCVN NLYFVCYQLL NVFNKLRWPI NYIYFWYSLL YLIGRTAFVF LTAADINEES KRGL GVLRR VSSRSWCVEV ERLIFQMTTQ TVALSGKKFY FLTRRLLFGM AGTIVTYELV LLQFDEPNRR KGLQPLCA

UniProtKB: Gustatory receptor for sugar taste 64a

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.4
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 52.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 1.5 µm / Nominal defocus min: 0.8 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Startup modelType of model: NONE
Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.5 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 372952
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD

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