Journal: EMBO J / Year: 2017 Title: Structure of a VirD4 coupling protein bound to a VirB type IV secretion machinery. Authors: Adam Redzej / Marta Ukleja / Sarah Connery / Martina Trokter / Catarina Felisberto-Rodrigues / Adam Cryar / Konstantinos Thalassinos / Richard D Hayward / Elena V Orlova / Gabriel Waksman / Abstract: Type IV secretion (T4S) systems are versatile bacterial secretion systems mediating transport of protein and/or DNA T4S systems are generally composed of 11 VirB proteins and 1 VirD protein (VirD4). ...Type IV secretion (T4S) systems are versatile bacterial secretion systems mediating transport of protein and/or DNA T4S systems are generally composed of 11 VirB proteins and 1 VirD protein (VirD4). The VirB1-11 proteins assemble to form a secretion machinery and a pilus while the VirD4 protein is responsible for substrate recruitment. The structure of VirD4 in isolation is known; however, its structure bound to the VirB1-11 apparatus has not been determined. Here, we purify a T4S system with VirD4 bound, define the biochemical requirements for complex formation and describe the protein-protein interaction network in which VirD4 is involved. We also solve the structure of this complex by negative stain electron microscopy, demonstrating that two copies of VirD4 dimers locate on both sides of the apparatus, in between the VirB4 ATPases. Given the central role of VirD4 in type IV secretion, our study provides mechanistic insights on a process that mediates the dangerous spread of antibiotic resistance genes among bacterial populations.
History
Deposition
Feb 3, 2017
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Header (metadata) release
Mar 8, 2017
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Map release
Jan 17, 2018
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Update
Jul 31, 2019
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Current status
Jul 31, 2019
Processing site: PDBe / Status: Released
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Structure visualization
Movie
Surface view with section colored by density value
Type: NEGATIVE / Material: 2 % uranyl acetate / Details: Staining for 3 min
Grid
Model: type B 400, Agar Scientific / Material: COPPER / Support film - Material: CARBON / Support film - topology: CONTINUOUS / Support film - Film thickness: 4.0 nm / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Atmosphere: AIR
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Electron microscopy
Microscope
FEI TECNAI F20
Image recording
Film or detector model: DIRECT ELECTRON DE-20 (5k x 3k) / Detector mode: INTEGRATING / Digitization - Frames/image: 2-19 / Average exposure time: 2.5 sec. / Average electron dose: 30.0 e/Å2
Electron beam
Acceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN
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