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- EMDB-35588: Cryo-EM structure of CasJ19-crRNA-DNA complex at 3.1 Angstroms re... -
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Open data
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Basic information
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Title | Cryo-EM structure of CasJ19-crRNA-DNA complex at 3.1 Angstroms resolution, Conformation 1 | |||||||||
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![]() | CRISPR-CasJ19 / RNA BINDING PROTEIN/RNA/DNA / RNA BINDING PROTEIN-RNA-DNA complex | |||||||||
Biological species | unidentified (others) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.1 Å | |||||||||
![]() | Zhang X / Duan Z / Zhu J | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Cryo-EM structure of CasJ19-crRNA-DNA complex at 3.1 Angstroms resolution, Conformation 1 Authors: Zhang X / Duan Z / Zhu J | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 25.5 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 10.9 KB 10.9 KB | Display Display | ![]() |
Images | ![]() | 107.9 KB | ||
Masks | ![]() | 27 MB | ![]() | |
Filedesc metadata | ![]() | 3.6 KB | ||
Others | ![]() ![]() | 25.1 MB 25.1 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 767.5 KB | Display | ![]() |
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Full document | ![]() | 767.1 KB | Display | |
Data in XML | ![]() | 10.4 KB | Display | |
Data in CIF | ![]() | 12.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
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Links
EMDB pages | ![]() ![]() |
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Map
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Voxel size | X=Y=Z: 1.32 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Mask #1
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Projections & Slices |
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Density Histograms |
-Half map: #1
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Projections & Slices |
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Density Histograms |
-Half map: #2
File | emd_35588_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
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Sample components
-Entire : Ternary complex of casJ19 with crRNA and target DNA, Conformation 1
Entire | Name: Ternary complex of casJ19 with crRNA and target DNA, Conformation 1 |
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Components |
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-Supramolecule #1: Ternary complex of casJ19 with crRNA and target DNA, Conformation 1
Supramolecule | Name: Ternary complex of casJ19 with crRNA and target DNA, Conformation 1 type: complex / ID: 1 / Parent: 0 |
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Source (natural) | Organism: unidentified (others) |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 7.4 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 50.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 5.0 µm / Nominal defocus min: 1.2 µm |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
Startup model | Type of model: NONE |
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Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 3.1 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 242907 |
Initial angle assignment | Type: MAXIMUM LIKELIHOOD |
Final angle assignment | Type: MAXIMUM LIKELIHOOD |