EMDB-34725: NPC-trapped pre-60S particle

Basic information

Entry

Database: EMDB / ID: EMD-34725

• Title: NPC-trapped pre-60S particle
• Map data: NPC-trapped pre-60S particle

Sample

• Complex: NPC-trapped pre-60S ribosome
  • Protein or peptide: x 54 types
  • RNA: x 3 types
• Ligand: x 1 types

• Keywords: pre-60S ribosome / RIBOSOME

Function / homology

Function:

nuclear RNA export factor complex / regulation of ribosomal subunit export from nucleus / nuclear pore central transport channel / Hydrolases / tRNA re-export from nucleus / Transport of Mature mRNA derived from an Intron-Containing Transcript / tRNA export from nucleus / positive regulation of ATP-dependent activity / U4 snRNA binding / maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / regulation of polysaccharide biosynthetic process / tRNA processing / poly(A)+ mRNA export from nucleus / maturation of 5.8S rRNA / transporter complex / cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / response to cycloheximide / porin activity / lipopolysaccharide transport / SRP-dependent cotranslational protein targeting to membrane / GTP hydrolysis and joining of the 60S ribosomal subunit / ribosomal large subunit binding / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / ATPase activator activity / Formation of a pool of free 40S subunits / preribosome, large subunit precursor / nuclear-transcribed mRNA catabolic process / L13a-mediated translational silencing of Ceruloplasmin expression / Gram-negative-bacterium-type cell outer membrane assembly / translational elongation / ribosomal large subunit export from nucleus / U5 snRNA binding / U2 snRNA binding / U6 snRNA binding / nuclear pore / ribosomal subunit export from nucleus / mRNA export from nucleus / regulation of translational fidelity / U1 snRNA binding / protein-RNA complex assembly / maturation of LSU-rRNA / ribosomal small subunit export from nucleus / Neutrophil degranulation / translation initiation factor activity / assembly of large subunit precursor of preribosome / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / ribosomal large subunit biogenesis / cytosolic ribosome assembly / maturation of SSU-rRNA / macroautophagy / small-subunit processome / maintenance of translational fidelity / cell outer membrane / metallopeptidase activity / rRNA processing / protein transport / ribosome biogenesis / ATPase binding / 5S rRNA binding / ribosomal large subunit assembly / large ribosomal subunit rRNA binding / protein-macromolecule adaptor activity / cytosolic large ribosomal subunit / nucleic acid binding / cytoplasmic translation / tRNA binding / negative regulation of translation / rRNA binding / structural constituent of ribosome / ribosome / translation / response to antibiotic / mRNA binding / GTPase activity / GTP binding / nucleolus / proteolysis / RNA binding / zinc ion binding / nucleoplasm / metal ion binding / nucleus / cytosol / cytoplasm

Domain/homology:

: / Nuclear pore RNA shuttling protein Mtr2 / Nuclear pore RNA shuttling protein Mtr2 / Mex67, RNA recognition motif / RNA recognition motif / Ribosome biogenesis protein Alb1 / Alb1 / Translation machinery-associated protein 16 / Tma16 superfamily / Translation machinery-associated protein 16 / TAP C-terminal (TAP-C) domain / Nmd3, N-terminal / Ribosomal export protein Nmd3 / : / : / TAP C-terminal domain / NMD3 family / NMD3 OB-fold domain / NMD3 SH3 domain / NXF1/2/3/5 leucine-rich repeat domain / TAP C-terminal (TAP-C) domain profile. / C-terminal domain of vertebrate Tap protein / Nuclear RNA export factor / Nuclear RNA export factor, NTF2 domain / Nuclear transport factor 2, eukaryote / Nuclear transport factor 2 domain profile. / : / : / Domain of unknown function DUF2423 / YBL028C ribosome biogenesis factor, N-terminal domain / Nucleolar GTP-binding protein 2, N-terminal domain / Nucleolar GTP-binding protein 2 / NGP1NT (NUC091) domain / Nuclear transport factor 2 domain / Zinc-finger double-stranded RNA-binding / Zinc finger, double-stranded RNA binding / : / Ribosomal biogenesis NSA2 family / GTP-binding protein, orthogonal bundle domain superfamily / Ribosome assembly factor Mrt4 / : / NOG, C-terminal / Nucleolar GTP-binding protein 1 / NOGCT (NUC087) domain / Nucleolar GTP-binding protein 1, Rossman-fold domain / NOG1, N-terminal helical domain / Nucleolar GTP-binding protein 1 (NOG1) / NOG1 N-terminal helical domain / Circularly permuted (CP)-type guanine nucleotide-binding (G) domain / Circularly permuted (CP)-type guanine nucleotide-binding (G) domain profile. / Matrin/U1-C-like, C2H2-type zinc finger / U1-like zinc finger / OBG-type guanine nucleotide-binding (G) domain / OBG-type guanine nucleotide-binding (G) domain profile. / Translation initiation factor IF6 / eIF-6 family / translation initiation factor 6 / 50S ribosome-binding GTPase / LptD, C-terminal / LPS-assembly protein LptD / : / LPS transport system D / Organic solvent tolerance-like, N-terminal / LptA/(LptD N-terminal domain) LPS transport protein / Creatinase/aminopeptidase-like / UBA-like superfamily / GTP binding domain / Group II dsDNA virus coat/capsid protein / 50S ribosomal protein L10, insertion domain superfamily / : / 60S ribosomal protein L10P, insertion domain / Insertion domain in 60S ribosomal protein L10P / metallochaperone-like domain / TRASH domain / Ribosomal protein L1, conserved site / NTF2-like domain superfamily / Ribosomal protein L1 signature. / Ribosomal protein L1 / Ribosomal protein L29e / Ribosomal L29e protein family / Ribosomal protein L22e / Ribosomal protein L22e superfamily / Ribosomal L22e protein family / Ribosomal protein L27e, conserved site / Ribosomal protein L27e signature. / Ribosomal protein L38e / Ribosomal protein L38e superfamily / Ribosomal L38e protein family / Ribosomal protein L1, 3-layer alpha/beta-sandwich / Ribosomal protein L44e signature. / Ribosomal protein L19/L19e conserved site / Ribosomal protein L19, eukaryotic / Ribosomal protein L19e signature. / : / 60S ribosomal protein L18a/ L20, eukaryotes / Ribosomal protein L6e signature. / : / Ribosomal protein L44e / Ribosomal protein L44 / Ribosomal protein L34e, conserved site

Component:

Large ribosomal subunit protein uL15 / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein eL39 / Large ribosomal subunit protein uL30A / Large ribosomal subunit protein uL6A / Large ribosomal subunit protein uL22A / Large ribosomal subunit protein uL24A / Large ribosomal subunit protein eL33A / Large ribosomal subunit protein eL36A / Large ribosomal subunit protein eL29 / Large ribosomal subunit protein eL15A / Large ribosomal subunit protein eL22A / Large ribosomal subunit protein uL5A / Large ribosomal subunit protein eL27A / Large ribosomal subunit protein eL31A / Large ribosomal subunit protein eL20A / Large ribosomal subunit protein eL43A / Large ribosomal subunit protein eL42A / Large ribosomal subunit protein uL14A / Large ribosomal subunit protein uL1A / Large ribosomal subunit protein uL2A / Large ribosomal subunit protein eL18A / Large ribosomal subunit protein uL11A / Large ribosomal subunit protein eL19A / Large ribosomal subunit protein uL29A / Large ribosomal subunit protein uL4A / Putative major capsid protein p72 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein eL8A / Large ribosomal subunit protein uL18 / Large ribosomal subunit protein uL13A / Ribosome assembly factor MRT4 / mRNA transport regulator MTR2 / Large ribosomal subunit protein eL14A / Large ribosomal subunit protein eL32 / UPF0642 protein YBL028C / 60S ribosomal export protein NMD3 / Shuttling pre-60S factor ECM1 / Ribosome biogenesis protein NSA2 / Ribosome biogenesis protein ALB1 / Large ribosomal subunit protein eL37A / Large ribosomal subunit protein eL38 / Nucleolar GTP-binding protein 2 / Large ribosomal subunit protein eL34A / Large ribosomal subunit protein eL6A / Large ribosomal subunit protein eL21A / Nucleolar GTP-binding protein 1 / Probable metalloprotease ARX1 / Ribosome biogenesis protein RLP24 / Bud site selection protein 20 / Translation machinery-associated protein 16 / Eukaryotic translation initiation factor 6 / LPS-assembly protein LptD / mRNA export factor MEX67

• Biological species: Saccharomyces cerevisiae S288C (yeast)
• Method: single particle reconstruction / cryo EM / Resolution: 2.64 Å
• Authors: Li ZQ / Chen SJ / Sui SF

Funding support

China, 1 items

Organization	Grant number	Country
National Natural Science Foundation of China (NSFC)		China

• Citation: Journal: Nature / Year: 2023; Title: Nuclear export of pre-60S particles through the nuclear pore complex.; Authors: Zongqiang Li / Shuaijiabin Chen / Liang Zhao / Guoqiang Huang / Huiqin Xu / Xiaoyun Yang / Peiyi Wang / Ning Gao / Sen-Fang Sui / ; Abstract: The nuclear pore complex (NPC) is the bidirectional gate that mediates the exchange of macromolecules or their assemblies between nucleus and cytoplasm. The assembly intermediates of the ribosomal subunits, pre-60S and pre-40S particles, are among the largest cargoes of the NPC and the export of these gigantic ribonucleoproteins requires numerous export factors. Here we report the cryo-electron microscopy structure of native pre-60S particles trapped in the channel of yeast NPCs. In addition to known assembly factors, multiple factors with export functions are also included in the structure. These factors in general bind to either the flexible regions or subunit interface of the pre-60S particle, and virtually form many anchor sites for NPC binding. Through interactions with phenylalanine-glycine (FG) repeats from various nucleoporins of NPC, these factors collectively facilitate the passage of the pre-60S particle through the central FG repeat network of the NPC. Moreover, in silico analysis of the axial and radial distribution of pre-60S particles within the NPC shows that a single NPC can take up to four pre-60S particles simultaneously, and pre-60S particles are enriched in the inner ring regions close to the wall of the NPC with the solvent-exposed surface facing the centre of the nuclear pore. Our data suggest a translocation model for the export of pre-60S particles through the NPC.

History

Deposition	Nov 12, 2022	-
Header (metadata) release	Apr 12, 2023	-
Map release	Apr 12, 2023	-
Update	Nov 13, 2024	-
Current status	Nov 13, 2024	Processing site: PDBj / Status: Released

Map

• File: Download / File: emd_34725.map.gz / Format: CCP4 / Size: 1.9 GB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Annotation: NPC-trapped pre-60S particle
• Voxel size: X=Y=Z: 0.668 Å

Density

Contour Level	By AUTHOR: 0.12
Minimum - Maximum	-0.4946383 - 1.3733753
Average (Standard dev.)	0.0016723165 (±0.037945535)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 800 800 800 Spacing 800 800 800
Cell	A=B=C: 534.39996 Å α=β=γ: 90.0 °

Supplemental data

Half map: NPC-trapped pre-60S particle half B map

• File: emd_34725_half_map_1.map
• Annotation: NPC-trapped pre-60S particle half B map

Half map: NPC-trapped pre-60S particle half A map

• File: emd_34725_half_map_2.map
• Annotation: NPC-trapped pre-60S particle half A map

Sample components

Entire : NPC-trapped pre-60S ribosome

• Entire: Name: NPC-trapped pre-60S ribosome

Components

• Complex: NPC-trapped pre-60S ribosome
  • Protein or peptide: 60S ribosomal protein L35-A
  • Protein or peptide: 60S ribosomal protein L34-A
  • Protein or peptide: 60S ribosomal protein L29
  • Protein or peptide: 60S ribosomal protein L2-A
  • Protein or peptide: 60S ribosomal protein L23-A
  • Protein or peptide: 60S ribosomal protein L16-A
  • Protein or peptide: 60S ribosomal protein L33-A
  • Protein or peptide: 60S ribosomal protein L6-A
  • Protein or peptide: Nucleolar GTP-binding protein 2
  • Protein or peptide: Nucleolar GTP-binding protein 1
  • Protein or peptide: Ribosome biogenesis protein RLP24
  • Protein or peptide: 60S ribosomal protein L30
  • Protein or peptide: 60S ribosomal protein L3
  • Protein or peptide: Eukaryotic translation initiation factor 6
  • Protein or peptide: 60S ribosomal protein L22-A
  • Protein or peptide: 60S ribosomal protein L31-A
  • Protein or peptide: 60S ribosomal protein L32
  • Protein or peptide: Shuttling pre-60S factor ECM1
  • Protein or peptide: Ribosome biogenesis protein ALB1
  • Protein or peptide: mRNA transport regulator MTR2
  • Protein or peptide: 60S ribosomal protein L13-A
  • Protein or peptide: YEAST 60S ribosomal protein L12-A
  • Protein or peptide: mRNA export factor MEX67
  • Protein or peptide: 60S ribosomal protein L4-A
  • Protein or peptide: 60S ribosomal protein L19-A
  • Protein or peptide: 60S ribosomal protein L14-A
  • Protein or peptide: 60S ribosomal protein L42-A
  • Protein or peptide: Probable metalloprotease ARX1
  • Protein or peptide: 60S ribosomal protein L26-A
  • Protein or peptide: 60S ribosomal export protein NMD3
  • Protein or peptide: 60S ribosomal protein L43-A
  • Protein or peptide: 60S ribosomal protein L20-A
  • Protein or peptide: 60S ribosomal protein L36-A
  • Protein or peptide: 60S ribosomal protein L9-A
  • Protein or peptide: 60S ribosomal protein L25
  • Protein or peptide: Ribosome biogenesis protein NSA2
  • RNA: 25S ribosomal RNA
  • RNA: 5S ribosomal RNA
  • Protein or peptide: Ribosome assembly factor MRT4
  • Protein or peptide: 60S ribosomal protein L37-A
  • Protein or peptide: 60S ribosomal protein L39
  • Protein or peptide: 60S ribosomal protein L8-A
  • Protein or peptide: 60S ribosomal protein L18-A
  • Protein or peptide: 60S ribosomal protein L21-A
  • Protein or peptide: Translation machinery-associated protein 16
  • RNA: 5.8S ribosomal RNA
  • Protein or peptide: 60S ribosomal protein L15-A
  • Protein or peptide: UPF0642 protein YBL028C
  • Protein or peptide: 60S ribosomal protein L27-A
  • Protein or peptide: 60S ribosomal protein L38
  • Protein or peptide: YEAST 60S ribosomal protein L1-A
  • Protein or peptide: 60S ribosomal protein L7-A
  • Protein or peptide: 60S ribosomal protein L17-A
  • Protein or peptide: 60S ribosomal protein L28
  • Protein or peptide: Bud site selection protein 20
  • Protein or peptide: 60S ribosomal protein L11-A
  • Protein or peptide: 60S ribosomal protein L5
• Ligand: MAGNESIUM ION

Supramolecule #1: NPC-trapped pre-60S ribosome

• Supramolecule: Name: NPC-trapped pre-60S ribosome / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#57
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)

Macromolecule #1: 60S ribosomal protein L35-A

• Macromolecule: Name: 60S ribosomal protein L35-A / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 13.94264 KDa

Sequence

String:

MAGVKAYELR TKSKEQLASQ LVDLKKELAE LKVQKLSRPS LPKIKTVRKS IACVLTVINE QQREAVRQLY KGKKYQPKDL RAKKTRALR RALTKFEASQ VTEKQRKKQI AFPQRKYAIK A

UniProtKB: Large ribosomal subunit protein uL29A

Macromolecule #2: 60S ribosomal protein L34-A

• Macromolecule: Name: 60S ribosomal protein L34-A / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 13.673196 KDa

Sequence

String:

MAQRVTFRRR NPYNTRSNKI KVVKTPGGIL RAQHVKKLAT RPKCGDCGSA LQGISTLRPR QYATVSKTHK TVSRAYGGSR CANCVKERI IRAFLIEEQK IVKKVVKEQT EAAKKSEKKA KK

UniProtKB: Large ribosomal subunit protein eL34A

Macromolecule #3: 60S ribosomal protein L29

• Macromolecule: Name: 60S ribosomal protein L29 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 6.691884 KDa

Sequence

String:

MAKSKNHTAH NQTRKAHRNG IKKPKTYKYP SLKGVDPKFR RNHKHALHGT AKALAAAKK

UniProtKB: Large ribosomal subunit protein eL29

Macromolecule #4: 60S ribosomal protein L2-A

• Macromolecule: Name: 60S ribosomal protein L2-A / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 27.463574 KDa

Sequence

String:

MGRVIRNQRK GAGSIFTSHT RLRQGAAKLR TLDYAERHGY IRGIVKQIVH DSGRGAPLAK VVFRDPYKYR LREEIFIANE GVHTGQFIY AGKKASLNVG NVLPLGSVPE GTIVSNVEEK PGDRGALARA SGNYVIIIGH NPDENKTRVR LPSGAKKVIS S DARGVIGV IAGGGRVDKP LLKAGRAFHK YRLKRNSWPK TRGVAMNPVD HPHGGGNHQH IGKASTISRG AVSGQKAGLI AA RRTGLLR GSQKTQD

UniProtKB: Large ribosomal subunit protein uL2A

Macromolecule #5: 60S ribosomal protein L23-A

• Macromolecule: Name: 60S ribosomal protein L23-A / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 14.49395 KDa

Sequence

String:

MSGNGAQGTK FRISLGLPVG AIMNCADNSG ARNLYIIAVK GSGSRLNRLP AASLGDMVMA TVKKGKPELR KKVMPAIVVR QAKSWRRRD GVFLYFEDNA GVIANPKGEM KGSAITGPVG KECADLWPRV ASNSGVVV

UniProtKB: Large ribosomal subunit protein uL14A

Macromolecule #6: 60S ribosomal protein L16-A

• Macromolecule: Name: 60S ribosomal protein L16-A / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 22.247227 KDa

Sequence

String:

MSVEPVVVID GKGHLVGRLA SVVAKQLLNG QKIVVVRAEE LNISGEFFRN KLKYHDFLRK ATAFNKTRGP FHFRAPSRIF YKALRGMVS HKTARGKAAL ERLKVFEGIP PPYDKKKRVV VPQALRVLRL KPGRKYTTLG KLSTSVGWKY EDVVAKLEAK R KVSSAEYY AKKRAFTKKV ASANATAAES DVAKQLAALG Y

UniProtKB: Large ribosomal subunit protein uL13A

Macromolecule #7: 60S ribosomal protein L33-A

• Macromolecule: Name: 60S ribosomal protein L33-A / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 12.17713 KDa

Sequence

String:

MAESHRLYVK GKHLSYQRSK RVNNPNVSLI KIEGVATPQD AQFYLGKRIA YVYRASKEVR GSKIRVMWGK VTRTHGNSGV VRATFRNNL PAKTFGASVR IFLYPSNI

UniProtKB: Large ribosomal subunit protein eL33A

Macromolecule #8: 60S ribosomal protein L6-A

• Macromolecule: Name: 60S ribosomal protein L6-A / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 20.000564 KDa

Sequence

String:

MSAQKAPKWY PSEDVAALKK TRKAARPQKL RASLVPGTVL ILLAGRFRGK RVVYLKHLED NTLLISGPFK VNGVPLRRVN ARYVIATST KVSVEGVNVE KFNVEYFAKE KLTKKEKKEA NLFPEQQNKE IKAERVEDQK VVDKALIAEI KKTPLLKQYL S ASFSLKNG DKPHMLKF

UniProtKB: Large ribosomal subunit protein eL6A

Macromolecule #9: Nucleolar GTP-binding protein 2

• Macromolecule: Name: Nucleolar GTP-binding protein 2 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 55.58559 KDa

Sequence

String:

MGTGKKEKSR RIREGDTKDG NLRVKGENFY RDSKRVKFLN MYTSGKEIRN KKGNLIRAAS FQDSTIPDAR VQPDRRWFGN TRVISQDAL QHFRSALGET QKDTYQVLLR RNKLPMSLLE EKDADESPKA RILDTESYAD AFGPKAQRKR PRLAASNLED L VKATNEDI TKYEEKQVLD ATLGLMGNQE DKENGWTSAA KEAIFSKGQS KRIWNELYKV IDSSDVVIHV LDARDPLGTR CK SVEEYMK KETPHKHLIY VLNKCDLVPT WVAAAWVKHL SKERPTLAFH ASITNSFGKG SLIQLLRQFS QLHTDRKQIS VGF IGYPNT GKSSIINTLR KKKVCQVAPI PGETKVWQYI TLMKRIFLID CPGIVPPSSK DSEEDILFRG VVRVEHVTHP EQYI PGVLK RCQVKHLERT YEISGWKDAT EFIEILARKQ GRLLKGGEPD ESGVSKQILN DFNRGKIPWF VLPPEKEGEE KPKKK EVEK TA

UniProtKB: Nucleolar GTP-binding protein 2

Macromolecule #10: Nucleolar GTP-binding protein 1

• Macromolecule: Name: Nucleolar GTP-binding protein 1 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 74.531227 KDa

Sequence

String:

MQLSWKDIPT VAPANDLLDI VLNRTQRKTP TVIRPGFKIT RIRAFYMRKV KYTGEGFVEK FEDILKGFPN INDVHPFHRD LMDTLYEKN HYKISLAAIS RAKSLVEQVA RDYVRLLKFG QSLFQCKQLK RAALGRMATI VKKLRDPLAY LEQVRQHIGR L PSIDPNTR TLLICGYPNV GKSSFLRCIT KSDVDVQPYA FTTKSLYVGH FDYKYLRFQA IDTPGILDRP TEEMNNIEMQ SI YAIAHLR SCVLYFMDLS EQCGFTIEAQ VKLFHSIKPL FANKSVMVVI NKTDIIRPED LDEERAQLLE SVKEVPGVEI MTS SCQLEE NVMEVRNKAC EKLLASRIEN KLKSQSRINN VLNKIHVAQP QARDDVKRTP FIPESVKNLK KYDPEDPNRR KLAR DIEAE NGGAGVFNVN LKDKYLLEDD EWKNDIMPEI LDGKNVYDFL DPEIAAKLQA LEEEEEKLEN EGFYNSDDEE EIYDG FEAS EVDDIKEKAA WIRNRQKTMI AEARNRKSLK NKAIMPRSKL TKSFGKMEEH MSTLGHDMSA LQDKQNRAAR KNRYVE RGS DVVFGDQDAL TASTENGVKL RQTDRLLDGV ADGSMRSKAD RMAKMERRER NRHAKQGESD RHNAVSLSKH LFSGKRG VG KTDFR

UniProtKB: Nucleolar GTP-binding protein 1

Macromolecule #11: Ribosome biogenesis protein RLP24

• Macromolecule: Name: Ribosome biogenesis protein RLP24 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 24.02765 KDa

Sequence

String:

MRIYQCHFCS SPCYPGHGIM FVRNDAKEFR FCRSKCHKAF KQRRNPRKLK WTKAFRKAAG KELAVDSTLT FAQRRNVPVR YNRELVATT LKAMARIEEI RQKRERAFYK NRMRGNKEKD FLRDKKLVES NPELLRIREV EIARKLAKEQ ERAESVSEQE E SEEEEEDM EIDSDEEEEE QLEKQKILLK NRRRNTKKIA F

UniProtKB: Ribosome biogenesis protein RLP24

Macromolecule #12: 60S ribosomal protein L30

• Macromolecule: Name: 60S ribosomal protein L30 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 11.430364 KDa

Sequence

String:

MAPVKSQESI NQKLALVIKS GKYTLGYKST VKSLRQGKSK LIIIAANTPV LRKSELEYYA MLSKTKVYYF QGGNNELGTA VGKLFRVGV VSILEAGDSD ILTTLA

UniProtKB: Putative major capsid protein p72

Macromolecule #13: 60S ribosomal protein L3

• Macromolecule: Name: 60S ribosomal protein L3 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 43.850793 KDa

Sequence

String:

MSHRKYEAPR HGHLGFLPRK RAASIRARVK AFPKDDRSKP VALTSFLGYK AGMTTIVRDL DRPGSKFHKR EVVEAVTVVD TPPVVVVGV VGYVETPRGL RSLTTVWAEH LSDEVKRRFY KNWYKSKKKA FTKYSAKYAQ DGAGIERELA RIKKYASVVR V LVHTQIRK TPLAQKKAHL AEIQLNGGSI SEKVDWAREH FEKTVAVDSV FEQNEMIDAI AVTKGHGFEG VTHRWGTKKL PR KTHRGLR KVACIGAWHP AHVMWSVARA GQRGYHSRTS INHKIYRVGK GDDEANGATS FDRTKKTITP MGGFVHYGEI KND FIMVKG CIPGNRKRIV TLRKSLYTNT SRKALEEVSL KWIDTASKFG KGRFQTPAEK HAFMGTLKKD L

UniProtKB: Large ribosomal subunit protein uL3

Macromolecule #14: Eukaryotic translation initiation factor 6

• Macromolecule: Name: Eukaryotic translation initiation factor 6 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 26.476605 KDa

Sequence

String:

MATRTQFENS NEIGVFSKLT NTYCLVAVGG SENFYSAFEA ELGDAIPIVH TTIAGTRIIG RMTAGNRRGL LVPTQTTDQE LQHLRNSLP DSVKIQRVEE RLSALGNVIC CNDYVALVHP DIDRETEELI SDVLGVEVFR QTISGNILVG SYCSLSNQGG L VHPQTSVQ DQEELSSLLQ VPLVAGTVNR GSSVVGAGMV VNDYLAVTGL DTTAPELSVI ESIFRLQDAQ PESISGNLRD TL IETYS

UniProtKB: Eukaryotic translation initiation factor 6

Macromolecule #15: 60S ribosomal protein L22-A

• Macromolecule: Name: 60S ribosomal protein L22-A / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 13.711359 KDa

Sequence

String:

MAPNTSRKQK IAKTFTVDVS SPTENGVFDP ASYAKYLIDH IKVEGAVGNL GNAVTVTEDG TVVTVVSTAK FSGKYLKYLT KKYLKKNQL RDWIRFVSTK TNEYRLAFYQ VTPEEDEEED EE

UniProtKB: Large ribosomal subunit protein eL22A

Macromolecule #16: 60S ribosomal protein L31-A

• Macromolecule: Name: 60S ribosomal protein L31-A / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 12.980158 KDa

Sequence

String:

MAGLKDVVTR EYTINLHKRL HGVSFKKRAP RAVKEIKKFA KLHMGTDDVR LAPELNQAIW KRGVKGVEYR LRLRISRKRN EEEDAKNPL FSYVEPVLVA SAKGLQTVVV EEDA

UniProtKB: Large ribosomal subunit protein eL31A

Macromolecule #17: 60S ribosomal protein L32

• Macromolecule: Name: 60S ribosomal protein L32 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 14.809441 KDa

Sequence

String:

MASLPHPKIV KKHTKKFKRH HSDRYHRVAE NWRKQKGIDS VVRRRFRGNI SQPKIGYGSN KKTKFLSPSG HKTFLVANVK DLETLTMHT KTYAAEIAHN ISAKNRVVIL ARAKALGIKV TNPKGRLALE A

UniProtKB: Large ribosomal subunit protein eL32

Macromolecule #18: Shuttling pre-60S factor ECM1

• Macromolecule: Name: Shuttling pre-60S factor ECM1 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 23.934316 KDa

Sequence

String:

MWEQRRQKVV FSLTILVRYR LKQSMAKKIS KNSRAARQSD ALEPEVKDLS ELPRAEKTDL TNILIRTAAK NEALLEAKIS KKANKSKRG KKLNKKALED KLANSISSMD RDRLVKALNF TNRLDGKIAK SISRAKYIQN TRKAGWDSTN ETIKKELAFL N GGLSVQAK SASEGNAEKE DEEIPEVFDS LAEDNTVQKT PTNRFGVLPD DVEE

UniProtKB: Shuttling pre-60S factor ECM1

Macromolecule #19: Ribosome biogenesis protein ALB1

• Macromolecule: Name: Ribosome biogenesis protein ALB1 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 19.322037 KDa

Sequence

String:

MPSKNSINRP KLTSNLHHKV HSLNKKRAQR ERAGLLKPAR SSVNSKSGEI KSVALDLYFQ NKKNESQNST AVTLQNASSS PASITTRTL SKKRAKKIER NLKYATQRKL LVDASAKLED EMDIDLDGGK KVKENEKKSS LTLVKEALWS VIDDTASQGL I IENGQGTT LGGPFFP

UniProtKB: Ribosome biogenesis protein ALB1

Macromolecule #20: mRNA transport regulator MTR2

• Macromolecule: Name: mRNA transport regulator MTR2 / type: protein_or_peptide / ID: 20 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 20.802506 KDa

Sequence

String:

MNTNSNTMVM NDANQAQITA TFTKKILAHL DDPDSNKLAQ FVQLFNPNNC RIIFNATPFA QATVFLQMWQ NQVVQTQHAL TGVDYHAIP GSGTLICNVN CKVRFDESGR DKMGQDATVP IQPNNTGNRN RPNDMNKPRP LWGPYFGISL QLIIDDRIFR N DFNGVISG FNYNMVYKPE DSLLKI

UniProtKB: mRNA transport regulator MTR2

Macromolecule #21: 60S ribosomal protein L13-A

• Macromolecule: Name: 60S ribosomal protein L13-A / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 22.604164 KDa

Sequence

String:

MAISKNLPIL KNHFRKHWQE RVKVHFDQAG KKVSRRNARA TRAAKIAPRP LDLLRPVVRA PTVKYNRKVR AGRGFTLAEV KAAGLTAAY ARTIGIAVDH RRQNRNQEIF DANVQRLKEY QSKIIVFPRN GKAPEAEQVL SAAATFPIAQ PATDVEARAV Q DNGESAFR TLRLARSEKK FRGIREKRAR EKAEAEAEKK K

UniProtKB: LPS-assembly protein LptD

Macromolecule #22: YEAST 60S ribosomal protein L12-A

• Macromolecule: Name: YEAST 60S ribosomal protein L12-A / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 17.850621 KDa

Sequence

String:

MPPKFDPNEV KYLYLRAVGG EVGASAALAP KIGPLGLSPK KVGEDIAKAT KEFKGIKVTV QLKIQNRQAA ASVVPSASSL VITALKEPP RDRKKDKNVK HSGNIQLDEI IEIARQMRDK SFGRTLASVT KEILGTAQSV GCRVDFKNPH DIIEGINAGE I EIPEN

UniProtKB: Large ribosomal subunit protein uL11A

Macromolecule #23: mRNA export factor MEX67

• Macromolecule: Name: mRNA export factor MEX67 / type: protein_or_peptide / ID: 23 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 67.417477 KDa

Sequence

String:

MSGFHNVGNI NMMAQQQMQQ NRIKISVRNW QNATMNDLIN FISRNARVAV YDAHVEGPLV IGYVNSKAEA ESLMKWNGVR FAGSNLKFE LLDNNGASAG TSDTISFLRG VLLKRYDPQT KLLNLGALHS DPELIQKGVF SSISTQSKMF PAMMKLASTE K SLIVESVN LADNQLKDIS AISTLAQTFP NLKNLCLANN QIFRFRSLEV WKNKFKDLRE LLMTNNPITT DKLYRTEMLR LF PKLVVLD NVIVRDEQKL QTVYSLPMKI QQFFFENDAL GQSSTDFATN FLNLWDNNRE QLLNLYSPQS QFSVSVDSTI PPS TVTDSD QTPAFGYYMS SSRNISKVSS EKSIQQRLSI GQESINSIFK TLPKTKHHLQ EQPNEYSMET ISYPQINGFV ITLH GFFEE TGKPELESNK KTGKNNYQKN RRYNHGYNST SNNKLSKKSF DRTWVIVPMN NSVIIASDLL TVRAYSTGAW KTASI AIAQ PPQQQASVLP QVASMNPNIT TPPQPQPSVV PGGMSIPGAP QGAMVMAPTL QLPPDVQSRL NPVQLELLNK LHLETK LNA EYTFMLAEQS NWNYEVAIKG FQSSMNGIPR EAFVQF

UniProtKB: mRNA export factor MEX67

Macromolecule #24: 60S ribosomal protein L4-A

• Macromolecule: Name: 60S ribosomal protein L4-A / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 39.159125 KDa

Sequence

String:

MSRPQVTVHS LTGEATANAL PLPAVFSAPI RPDIVHTVFT SVNKNKRQAY AVSEKAGHQT SAESWGTGRA VARIPRVGGG GTGRSGQGA FGNMCRGGRM FAPTKTWRKW NVKVNHNEKR YATASAIAAT AVASLVLARG HRVEKIPEIP LVVSTDLESI Q KTKEAVAA LKAVGAHSDL LKVLKSKKLR AGKGKYRNRR WTQRRGPLVV YAEDNGIVKA LRNVPGVETA NVASLNLLQL AP GAHLGRF VIWTEAAFTK LDQVWGSETV ASSKVGYTLP SHIISTSDVT RIINSSEIQS AIRPAGQATQ KRTHVLKKNP LKN KQVLLR LNPYAKVFAA EKLGSKKAEK TGTKPAAVFT ETLKHD

UniProtKB: Large ribosomal subunit protein uL4A

Macromolecule #25: 60S ribosomal protein L19-A

• Macromolecule: Name: 60S ribosomal protein L19-A / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 21.762316 KDa

Sequence

String:

MANLRTQKRL AASVVGVGKR KVWLDPNETS EIAQANSRNA IRKLVKNGTI VKKAVTVHSK SRTRAHAQSK REGRHSGYGK RKGTREARL PSQVVWIRRL RVLRRLLAKY RDAGKIDKHL YHVLYKESKG NAFKHKRALV EHIIQAKADA QREKALNEEA E ARRLKNRA ARDRRAQRVA EKRDALLKED A

UniProtKB: Large ribosomal subunit protein eL19A

Macromolecule #26: 60S ribosomal protein L14-A

• Macromolecule: Name: 60S ribosomal protein L14-A / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 15.195066 KDa

Sequence

String:

MSTDSIVKAS NWRLVEVGRV VLIKKGQSAG KLAAIVEIID QKKVLIDGPK AGVPRQAINL GQVVLTPLTF ALPRGARTAT VSKKWAAAA VCEKWAASSW AKKIAQRERR AALTDFERFQ VMVLRKQKRY TVKKALAKA

UniProtKB: Large ribosomal subunit protein eL14A

Macromolecule #27: 60S ribosomal protein L42-A

• Macromolecule: Name: 60S ribosomal protein L42-A / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 12.246658 KDa

Sequence

String:

MVNVPKTRKT YCKGKTCRKH TQHKVTQYKA GKASLFAQGK RRYDRKQSGF GGQTKPVFHK KAKTTKKVVL RLECVKCKTR AQLTLKRCK HFELGGEKKQ KGQALQF

UniProtKB: Large ribosomal subunit protein eL42A

Macromolecule #28: Probable metalloprotease ARX1

• Macromolecule: Name: Probable metalloprotease ARX1 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO / EC number: Hydrolases
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 65.290336 KDa

Sequence

String:

MALAISHEDT QILLKDKNIL QESVLNKYRT AGQIAQTALK YVTSLINDSY HSKTTQRQLT VPELCLLTDS FILTRLEQYY KNKVNERGI AIPTTIDIDQ ISGGWCPEID DTQNLLNWNK GKDSTFASSV TGTLRPGDLV KITLGVHIDG YTSEVSHTMV I YPVDETKP ILQPTGPLLG GKADAVAAAH IAMETVVALL ACALTPEKLP ASLGGTSSGI TGQLIRTIVD TIARSYNCGV VP GSRVRRI RRFLAGQNEG IVAEREYKGV VWTESHQEAD LLSNTDAKDL TVVDRGQSTP FTNVSAIPSD DFVVQSGEVY LID LKMASL EHCTKKGLVT LETVDSYTGK SHKAGELIAR PGAYVRDFAQ THILKLKTSR QLLTKIDKQG VYPFKLSHLS SNFP FVHEN EEELQSLKKD LKSFRLGMSE ISNNYLCVES PIQIARWVPW DHILKATNPN GNLSYDATST LTLPGHELPL PKLGV SAIK LKSLMNSTKE SISLPVAREC NTIVLCDSSV STTDRPELLR LTGGSKTCQP SWIHSQHELN PQDSIVQGIF QLATLA KDK RFGLLLKETQ PMKQKSVETS NGGVEETMKM

UniProtKB: Probable metalloprotease ARX1

Macromolecule #29: 60S ribosomal protein L26-A

• Macromolecule: Name: 60S ribosomal protein L26-A / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 14.265784 KDa

Sequence

String:

MAKQSLDVSS DRRKARKAYF TAPSSQRRVL LSAPLSKELR AQYGIKALPI RRDDEVLVVR GSKKGQEGKI SSVYRLKFAV QVDKVTKEK VNGASVPINL HPSKLVITKL HLDKDRKALI QRKGGKLE

UniProtKB: Large ribosomal subunit protein uL24A

Macromolecule #30: 60S ribosomal export protein NMD3

• Macromolecule: Name: 60S ribosomal export protein NMD3 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 59.167047 KDa

Sequence

String:

MEFTPIDPHQ HQNAATLLCC NCGTPIDGST GLVMCYDCIK LTVDITQGIP REANISFCRN CERFLQPPGQ WIRAELESRE LLAICLRRL KGLTKVRLVD ASFIWTEPHS RRIRIKLTVQ GEAMTNTIIQ QTFEVEYIVI AMQCPDCARS YTTNTWRATV Q IRQKVPHK RTFLFLEQLI LKHNAHVDTI SISEAKDGLD FFYAQKNHAV KMIDFLNAVV PIKHKKSEEL ISQDTHTGAS TY KFSYSVE IVPICKDDLV VLPKKLAKSM GNISQFVLCS KISNTVQFMD PTTLQTADLS PSVYWRAPFN ALADVTQLVE FIV LDVDST GISRGNRVLA DITVARTSDL GVNDQVYYVR SHLGGICHAG DSVMGYFIAN SNYNSDLFDG LNIDYVPDVV LVKK LYQRK SKKSRHWKLK RMAKEHKDID ASLDYNSRAQ KQEMERAEKD YELFLQELEE DAELRQSVNL YKNREANVPP EEHEM DEDE DEDAPQINID ELLDELDEMT LEDGVENTPV ESQQ

UniProtKB: 60S ribosomal export protein NMD3

Macromolecule #31: 60S ribosomal protein L43-A

• Macromolecule: Name: 60S ribosomal protein L43-A / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 10.112952 KDa

Sequence

String:

MAKRTKKVGI TGKYGVRYGS SLRRQVKKLE IQQHARYDCS FCGKKTVKRG AAGIWTCSCC KKTVAGGAYT VSTAAAATVR STIRRLREM VEA

UniProtKB: Large ribosomal subunit protein eL43A

Macromolecule #32: 60S ribosomal protein L20-A

• Macromolecule: Name: 60S ribosomal protein L20-A / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 20.478852 KDa

Sequence

String:

MAHFKEYQVI GRRLPTESVP EPKLFRMRIF ASNEVIAKSR YWYFLQKLHK VKKASGEIVS INQINEAHPT KVKNFGVWVR YDSRSGTHN MYKEIRDVSR VAAVETLYQD MAARHRARFR SIHILKVAEI EKTADVKRQY VKQFLTKDLK FPLPHRVQKS T KTFSYKRP STFY

UniProtKB: Large ribosomal subunit protein eL20A

Macromolecule #33: 60S ribosomal protein L36-A

• Macromolecule: Name: 60S ribosomal protein L36-A / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 11.151259 KDa

Sequence

String:

MTVKTGIAIG LNKGKKVTSM TPAPKISYKK GAASNRTKFV RSLVREIAGL SPYERRLIDL IRNSGEKRAR KVAKKRLGSF TRAKAKVEE MNNIIAASRR H

UniProtKB: Large ribosomal subunit protein eL36A

Macromolecule #34: 60S ribosomal protein L9-A

• Macromolecule: Name: 60S ribosomal protein L9-A / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 21.605061 KDa

Sequence

String:

MKYIQTEQQI EVPEGVTVSI KSRIVKVVGP RGTLTKNLKH IDVTFTKVNN QLIKVAVHNG GRKHVAALRT VKSLVDNMIT GVTKGYKYK MRYVYAHFPI NVNIVEKDGA KFIEVRNFLG DKKIRNVPVR DGVTIEFSTN VKDEIVLSGN SVEDVSQNAA D LQQICRVR NKDIRKFLDG IYVSHKGFIT EDL

UniProtKB: Large ribosomal subunit protein uL6A

Macromolecule #35: 60S ribosomal protein L25

• Macromolecule: Name: 60S ribosomal protein L25 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 15.787612 KDa

Sequence

String:

MAPSAKATAA KKAVVKGTNG KKALKVRTSA TFRLPKTLKL ARAPKYASKA VPHYNRLDSY KVIEQPITSE TAMKKVEDGN ILVFQVSMK ANKYQIKKAV KELYEVDVLK VNTLVRPNGT KKAYVRLTAD YDALDIANRI GYI

UniProtKB: Large ribosomal subunit protein uL23

Macromolecule #36: Ribosome biogenesis protein NSA2

• Macromolecule: Name: Ribosome biogenesis protein NSA2 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 29.786783 KDa

Sequence

String:

MPQNDYIERH IKQHGKRLDH EERKRKREAR ESHKISERAQ KLTGWKGKQF AKKRYAEKVS MRKKIKAHEQ SKVKGSSKPL DTDGDALPT YLLDREQNNT AKAISSSIKQ KRLEKADKFS VPLPKVRGIS EEEMFKVIKT GKSRSKSWKR MITKHTFVGE G FTRRPVKM ERIIRPSALR QKKANVTHPE LGVTVFLPIL AVKKNPQSPM YTQLGVLTKG TIIEVNVSEL GMVTAGGKVV WG KYAQVTN EPDRDGCVNA VLLV

UniProtKB: Ribosome biogenesis protein NSA2

Macromolecule #39: Ribosome assembly factor MRT4

• Macromolecule: Name: Ribosome assembly factor MRT4 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 27.098012 KDa

Sequence

String:

MPRSKRSKLV TLAQTDKKGR ENKERIFDEV REALDTYRYV WVLHLDDVRT PVLQEIRTSW AGSKLIMGKR KVLQKALGEK REEEYKENL YQLSKLCSGV TGLLFTDEDV NTVKEYFKSY VRSDYSRPNT KAPLTFTIPE GIVYSRGGQI PAEEDVPMIH S LEPTMRNK FEIPTKIKAG KITIDSPYLV CTEGEKLDVR QALILKQFGI AASEFKVKVS AYYDNDSSTV ESTNINME

UniProtKB: Ribosome assembly factor MRT4

Macromolecule #40: 60S ribosomal protein L37-A

• Macromolecule: Name: 60S ribosomal protein L37-A / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 9.877395 KDa

Sequence

String:

MGKGTPSFGK RHNKSHTLCN RCGRRSFHVQ KKTCSSCGYP AAKTRSYNWG AKAKRRHTTG TGRMRYLKHV SRRFKNGFQT GSASKASA

UniProtKB: Large ribosomal subunit protein eL37A

Macromolecule #41: 60S ribosomal protein L39

• Macromolecule: Name: 60S ribosomal protein L39 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 6.35864 KDa

Sequence

String:

MAAQKSFRIK QKMAKAKKQN RPLPQWIRLR TNNTIRYNAK RRNWRRTKMN I

UniProtKB: Large ribosomal subunit protein eL39

Macromolecule #42: 60S ribosomal protein L8-A

• Macromolecule: Name: 60S ribosomal protein L8-A / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 28.17582 KDa

Sequence

String:

MAPGKKVAPA PFGAKSTKSN KTRNPLTHST PKNFGIGQAV QPKRNLSRYV KWPEYVRVQR QKKILSIRLK VPPTIAQFQY TLDRNTAAE TFKLFNKYRP ETAAEKKERL TKEAAAVAEG KSKQDASPKP YAVKYGLNHV VALIENKKAK LVLIANDVDP I ELVVFLPA LCKKMGVPYA IVKGKARLGT LVNQKTSAVA ALTEVRAEDE AALAKLVSTI DANFADKYDE VKKHWGGGIL GN KAQAKMD KRAKNSDSA

UniProtKB: Large ribosomal subunit protein eL8A

Macromolecule #43: 60S ribosomal protein L18-A

• Macromolecule: Name: 60S ribosomal protein L18-A / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 20.609252 KDa

Sequence

String:

MGIDHTSKQH KRSGHRTAPK SDNVYLKLLV KLYTFLARRT DAPFNKVVLK ALFLSKINRP PVSVSRIARA LKQEGAANKT VVVVGTVTD DARIFEFPKT TVAALRFTAG ARAKIVKAGG ECITLDQLAV RAPKGQNTLI LRGPRNSREA VRHFGMGPHK G KAPRILST GRKFERARGR RRSKGFKV

UniProtKB: Large ribosomal subunit protein eL18A

Macromolecule #44: 60S ribosomal protein L21-A

• Macromolecule: Name: 60S ribosomal protein L21-A / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 18.279266 KDa

Sequence

String:

MGKSHGYRSR TRYMFQRDFR KHGAVHLSTY LKVYKVGDIV DIKANGSIQK GMPHKFYQGK TGVVYNVTKS SVGVIINKMV GNRYLEKRL NLRVEHIKHS KCRQEFLERV KANAAKRAEA KAQGVAVQLK RQPAQPRESR IVSTEGNVPQ TLAPVPYETF I

UniProtKB: Large ribosomal subunit protein eL21A

Macromolecule #45: Translation machinery-associated protein 16

• Macromolecule: Name: Translation machinery-associated protein 16 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 20.883109 KDa

Sequence

String:

MPVTKSLSKL QKNLSKKGKN ITVHPKGRKY EKLVRATMRE DKIAAKKKLH QDKRVHELAR VKFMQDVVNS DTFKGQPIFD HAHTREFIQ SFIERDDTEL DELKKKRRSN RPPSNRQVLL QQRRDQELKE FKAGFLCPDL SDAKNMEFLR NWNGTFGLLN T LRLIRIND KGEQVVGGNE

UniProtKB: Translation machinery-associated protein 16

Macromolecule #47: 60S ribosomal protein L15-A

• Macromolecule: Name: 60S ribosomal protein L15-A / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 24.482357 KDa

Sequence

String:

MGAYKYLEEL QRKKQSDVLR FLQRVRVWEY RQKNVIHRAA RPTRPDKARR LGYKAKQGFV IYRVRVRRGN RKRPVPKGAT YGKPTNQGV NELKYQRSLR ATAEERVGRR AANLRVLNSY WVNQDSTYKY FEVILVDPQH KAIRRDARYN WICDPVHKHR E ARGLTATG KKSRGINKGH KFNNTKAGRR KTWKRQNTLS LWRYRK

UniProtKB: Large ribosomal subunit protein eL15A

Macromolecule #48: UPF0642 protein YBL028C

• Macromolecule: Name: UPF0642 protein YBL028C / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 12.435429 KDa

Sequence

String:

MAKSLRASSH LNAKSVKRRG VFQKAVDARE QRISDKLKED LLKQKLEDLK KKEEQGIDMD VDEKKSNEEA PRKKISTSGW RDGRHHTYK KAKLMKQSKK KTSFTRF

UniProtKB: UPF0642 protein YBL028C

Macromolecule #49: 60S ribosomal protein L27-A

• Macromolecule: Name: 60S ribosomal protein L27-A / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 15.56836 KDa

Sequence

String:

MAKFLKAGKV AVVVRGRYAG KKVVIVKPHD EGSKSHPFGH ALVAGIERYP LKVTKKHGAK KVAKRTKIKP FIKVVNYNHL LPTRYTLDV EAFKSVVSTE TFEQPSQREE AKKVVKKAFE ERHQAGKNQW FFSKLRF

UniProtKB: Large ribosomal subunit protein eL27A

Macromolecule #50: 60S ribosomal protein L38

• Macromolecule: Name: 60S ribosomal protein L38 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 8.845561 KDa

Sequence

String:

MAREITDIKQ FLELTRRADV KTATVKINKK LNKAGKPFRQ TKFKVRGSSS LYTLVINDAG KAKKLIQSLP PTLKVNRL

UniProtKB: Large ribosomal subunit protein eL38

Macromolecule #51: YEAST 60S ribosomal protein L1-A

• Macromolecule: Name: YEAST 60S ribosomal protein L1-A / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 24.524799 KDa

Sequence

String:

MSKITSSQVR EHVKELLKYS NETKKRNFLE TVELQVGLKN YDPQRDKRFS GSLKLPNCPR PNMSICIFGD AFDVDRAKSC GVDAMSVDD LKKLNKNKKL IKKLSKKYNA FIASEVLIKQ VPRLLGPQLS KAGKFPTPVS HNDDLYGKVT DVRSTIKFQL K KVLCLAVA VGNVEMEEDV LVNQILMSVN FFVSLLKKNW QNVGSLVVKS SMGPAFRLY

UniProtKB: Large ribosomal subunit protein uL1A

Macromolecule #52: 60S ribosomal protein L7-A

• Macromolecule: Name: 60S ribosomal protein L7-A / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 27.686281 KDa

Sequence

String:

MAAEKILTPE SQLKKSKAQQ KTAEQVAAER AARKAANKEK RAIILERNAA YQKEYETAER NIIQAKRDAK AAGSYYVEAQ HKLVFVVRI KGINKIPPKP RKVLQLLRLT RINSGTFVKV TKATLELLKL IEPYVAYGYP SYSTIRQLVY KRGFGKINKQ R VPLSDNAI IEANLGKYGI LSIDDLIHEI ITVGPHFKQA NNFLWPFKLS NPSGGWGVPR KFKHFIQGGS FGNREEFINK LV KSMN

UniProtKB: Large ribosomal subunit protein uL30A

Macromolecule #53: 60S ribosomal protein L17-A

• Macromolecule: Name: 60S ribosomal protein L17-A / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 20.589518 KDa

Sequence

String:

MARYGATSTN PAKSASARGS YLRVSFKNTR ETAQAINGWE LTKAQKYLEQ VLDHQRAIPF RRFNSSIGRT AQGKEFGVTK ARWPAKSVK FVQGLLQNAA ANAEAKGLDA TKLYVSHIQV NQAPKQRRRT YRAHGRINKY ESSPSHIELV VTEKEEAVAK A AEKKVVRL TSRQRGRIAA QKRIAA

UniProtKB: Large ribosomal subunit protein uL22A

Macromolecule #54: 60S ribosomal protein L28

• Macromolecule: Name: 60S ribosomal protein L28 / type: protein_or_peptide / ID: 54 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 16.761666 KDa

Sequence

String:

MPSRFTKTRK HRGHVSAGKG RIGKHRKHPG GRGMAGGQHH HRINMDKYHP GYFGKVGMRY FHKQQAHFWK PVLNLDKLWT LIPEDKRDQ YLKSASKETA PVIDTLAAGY GKILGKGRIP NVPVIVKARF VSKLAEEKIR AAGGVVELIA

UniProtKB: Large ribosomal subunit protein uL15

Macromolecule #55: Bud site selection protein 20

• Macromolecule: Name: Bud site selection protein 20 / type: protein_or_peptide / ID: 55 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 18.546982 KDa

Sequence

String:

MGRYSVKRYK TKRRTRDLDL IYNDLSTKES VQKLLNQPLD ETKPGLGQHY CIHCAKYMET AIALKTHLKG KVHKRRVKEL RGVPYTQEV SDAAAGYNLN KFLNRVQEIT QSVGPEKESN EALLKEHLDS TLANVKTTEP TLPWAAADAE ANTAAVTEAE S TASAST

UniProtKB: Bud site selection protein 20

Macromolecule #56: 60S ribosomal protein L11-A

• Macromolecule: Name: 60S ribosomal protein L11-A / type: protein_or_peptide / ID: 56 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 19.755691 KDa

Sequence

String:

MSAKAQNPMR DLKIEKLVLN ISVGESGDRL TRASKVLEQL SGQTPVQSKA RYTVRTFGIR RNEKIAVHVT VRGPKAEEIL ERGLKVKEY QLRDRNFSAT GNFGFGIDEH IDLGIKYDPS IGIFGMDFYV VMNRPGARVT RRKRCKGTVG NSHKTTKEDT V SWFKQKYD ADVLDK

UniProtKB: Large ribosomal subunit protein uL5A

Macromolecule #57: 60S ribosomal protein L5

• Macromolecule: Name: 60S ribosomal protein L5 / type: protein_or_peptide / ID: 57 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 33.764828 KDa

Sequence

String:

MAFQKDAKSS AYSSRFQTPF RRRREGKTDY YQRKRLVTQH KAKYNTPKYR LVVRFTNKDI ICQIISSTIT GDVVLAAAYS HELPRYGIT HGLTNWAAAY ATGLLIARRT LQKLGLDETY KGVEEVEGEY ELTEAVEDGP RPFKVFLDIG LQRTTTGARV F GALKGASD GGLYVPHSEN RFPGWDFETE EIDPELLRSY IFGGHVSQYM EELADDDEER FSELFKGYLA DDIDADSLED IY TSAHEAI RADPAFKPTE KKFTKEQYAA ESKKYRQTKL SKEERAARVA AKIAALAGQQ

UniProtKB: Large ribosomal subunit protein uL18

Macromolecule #37: 25S ribosomal RNA

• Macromolecule: Name: 25S ribosomal RNA / type: rna / ID: 37 / Number of copies: 1
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 1.0974938749999998 MDa

Sequence

String:

GUUUGACCUC AAAUCAGGUA GGAGUACCCG CUGAACUUAA GCAUAUCAAU AAGCGGAGGA AAAGAAACCA ACCGGGAUUG CCUUAGUAA CGGCGAGUGA AGCGGCAAAA GCUCAAAUUU GAAAUCUGGU ACCUUCGGUG CCCGAGUUGU AAUUUGGAGA G GGCAACUU UGGGGCCGUU CCUUGUCUAU GUUCCUUGGA ACAGGACGUC AUAGAGGGUG AGAAUCCCGU GUGGCGAGGA GU GCGGUUC UUUGUAAAGU GCCUUCGAAG AGUCGAGUUG UUUGGGAAUG CAGCUCUAAG UGGGUGGUAA AUUCCAUCUA AAG CUAAAU AUUGGCGAGA GACCGAUAGC GAACAAGUAC AGUGAUGGAA AGAUGAAAAG AACUUUGAAA AGAGAGUGAA AAAG UACGU GAAAUUGUUG AAAGGGAAGG GCAUUUGAUC AGACAUGGUG UUUUGUGCCC UCUGCUCCUU GUGGGUAGGG GAAUC UCGC AUUUCACUGG GCCAGCAUCA GUUUUGGUGG CAGGAUAAAU CCAUAGGAAU GUAGCUUGCC UCGGUAAGUA UUAUAG CCU GUGGGAAUAC UGCCAGCUGG GACUGAGGAC UGCGACGUAA GUCAAGGAUG CUGGCAUAAU GGUUAUAUGC CGCCCGU CU UGAAACACGG ACCAAGGAGU CUAACGUCUA UGCGAGUGUU UGGGUGUAAA ACCCAUACGC GUAAUGAAAG UGAACGUA G GUUGGGGCCU CGCAAGAGGU GCACAAUCGA CCGAUCCUGA UGUCUUCGGA UGGAUUUGAG UAAGAGCAUA GCUGUUGGG ACCCGAAAGA UGGUGAACUA UGCCUGAAUA GGGUGAAGCC AGAGGAAACU CUGGUGGAGG CUCGUAGCGG UUCUGACGUG CAAAUCGAU CGUCGAAUUU GGGUAUAGGG GCGAAAGACU AAUCGAACCA UCUAGUAGCU GGUUCCUGCC GAAGUUUCCC U CAGGAUAG CAGAAGCUCG UAUCAGUUUU AUGAGGUAAA GCGAAUGAUU AGAGGUUCCG GGGUCGAAAU GACCUUGACC UA UUCUCAA ACUUUAAAUA UGUAAGAAGU CCUUGUUACU UAAUUGAACG UGGACAUUUG AAUGAAGAGC UUUUAGUGGG CCA UUUUUG GUAAGCAGAA CUGGCGAUGC GGGAUGAACC GAACGUAGAG UUAAGGUGCC GGAAUACACG CUCAUCAGAC ACCA CAAAA GGUGUUAGUU CAUCUAGACA GCCGGACGGU GGCCAUGGAA GUCGGAAUCC GCUAAGGAGU GUGUAACAAC UCACC GGCC GAAUGAACUA GCCCUGAAAA UGGAUGGCGC UCAAGCGUGU UACCUAUACU CUACCGUCAG GGUUGAUAUG AUGCCC UGA CGAGUAGGCA GGCGUGGAGG UCAGUGACGA AGCCUAGACC GUAAGGUCGG GUCGAACGGC CUCUAGUGCA GAUCUUG GU GGUAGUAGCA AAUAUUCAAA UGAGAACUUU GAAGACUGAA GUGGGGAAAG GUUCCACGUC AACAGCAGUU GGACGUGG G UUAGUCGAUC CUAAGAGAUG GGGAAGCUCC GUUUCAAAGG CCUGAUUUUA UGCAGGCCAC CAUCGAAAGG GAAUCCGGU UAAGAUUCCG GAACCUGGAU AUGGAUUCUU CACGGUAACG UAACUGAAUG UGGAGACGUC GGCGCGAGCC CUGGGAGGAG UUAUCUUUU CUUCUUAACA GCUUAUCACC CCGGAAUUGG UUUAUCCGGA GAUGGGGUCU UAUGGCUGGA AGAGGCCAGC A CCUUUGCU GGCUCCGGUG CGCUUGUGAC GGCCCGUGAA AAUCCACAGG AAGGAAUAGU UUUCAUGCCA GGUCGUACUG AU AACCGCA GCAGGUCUCC AAGGUGAACA GCCUCUAGUU GAUAGAAUAA UGUAGAUAAG GGAAGUCGGC AAAAUAGAUC CGU AACUUC GGGAUAAGGA UUGGCUCUAA GGGUCGGGUA GUGAGGGCCU UGGUCAGACG CAGCGGGCGU GCUUGUGGAC UGCU UGGUG GGGCUUGCUC UGCUAGGCGG ACUACUUGCG UGCCUUGUUG UAGACGGCCU UGGUAGGUCU CUUGUAGACC GUCGC UUGC UACAAUUAAC GAUCAACUUA GAACUGGUAC GGACAAGGGG AAUCUGACUG UCUAAUUAAA ACAUAGCAUU GCGAUG GUC AGAAAGUGAU GUUGACGCAA UGUGAUUUCU GCCCAGUGCU CUGAAUGUCA AAGUGAAGAA AUUCAACCAA GCGCGGG UA AACGGCGGGA GUAACUAUGA CUCUCUUAAG GUAGCCAAAU GCCUCGUCAU CUAAUUAGUG ACGCGCAUGA AUGGAUUA A CGAGAUUCCC ACUGUCCCUA UCUACUAUCU AGCGAAACCA CAGCCAAGGG AACGGGCUUG GCAGAAUCAG CGGGGAAAG AAGACCCUGU UGAGCUUGAC UCUAGUUUGA CAUUGUGAAG AGACAUAGAG GGUGUAGAAU AAGUGGGAGC UUCGGCGCCA GUGAAAUAC CACUACCUUU AUAGUUUCUU UACUUAUUCA AUGAAGCGGA GCUGGAAUUC AUUUUCCACG UUCUAGCAUU C AAGGUCCC AUUCGGGGCU GAUCCGGGUU GAAGACAUUG UCAGGUGGGG AGUUUGGCUG GGGCGGCACA UCUGUUAAAC GA UAACGCA GAUGUCCUAA GGGGGGCUCA UGGAGAACAG AAAUCUCCAG UAGAACAAAA GGGUAAAAGC CCCCUUGAUU UUG AUUUUC AGUGUGAAUA CAAACCAUGA AAGUGUGGCC UAUCGAUCCU UUAGUCCCUC GGAAUUUGAG GCUAGAGGUG CCAG AAAAG UUACCACAGG GAUAACUGGC UUGUGGCAGU CAAGCGUUCA UAGCGACAUU GCUUUUUGAU UCUUCGAUGU CGGCU CUUC CUAUCAUACC GAAGCAGAAU UCGGUAAGCG UUGGAUUGUU CACCCACUAA UAGGGAACGU GAGCUGGGUU UAGACC GUC GUGAGACAGG UUAGUUUUAC CCUACUGAUG AAUGUUACCG CAAUAGUAAU UGAACUUAGU ACGAGAGGAA CAGUUCA UU CGGAUAAUUG GUUUUUGCGG CUGUCUGAUC AGGCAUUGCC GCGAAGCUAC CAUCCGCUGG AUUAUGGCUG AACGCCUC U AAGUCAGAAU CCAUGCUAGA ACGCGGUGAU UUCUUUGCUC CACACAAUAU AGAUGGAUAC GAAUAAGGCG UCCUUGUGG CGUCGCUGAA CCAUAGCAGG CUAGCAACGG UGCACUUGGC GGAAAGGCCU UGGGUGCUUG CUGGCGAAUU GCAAUGUCAU UUUGCGUGG GGAUAAAUCA UUUGUAUACG ACUUAGAUGU ACAACGGGGU AUUGUAAGCA GUAGAGUAGC CUUGUUGUUA C GAUCUGCU GAGAUUAAGC CUUUGUUGUC UGAUUUGU

GENBANK: GENBANK: NR_132207.1

Macromolecule #38: 5S ribosomal RNA

• Macromolecule: Name: 5S ribosomal RNA / type: rna / ID: 38 / Number of copies: 1
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 38.951105 KDa

Sequence

String:

GGUUGCGGCC AUAUCUACCA GAAAGCACCG UUUCCCGUCC GAUCAACUGU AGUUAAGCUG GUAAGAGCCU GACCGAGUAG UGUAGUGGG UGACCAUACG CGAAACUCAG GUGCUGCAAU CU

GENBANK: GENBANK: CP008604.1

Macromolecule #46: 5.8S ribosomal RNA

• Macromolecule: Name: 5.8S ribosomal RNA / type: rna / ID: 46 / Number of copies: 1
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 50.682922 KDa

Sequence

String:

AAACUUUCAA CAACGGAUCU CUUGGUUCUC GCAUCGAUGA AGAACGCAGC GAAAUGCGAU ACGUAAUGUG AAUUGCAGAA UUCCGUGAA UCAUCGAAUC UUUGAACGCA CAUUGCGCCC CUUGGUAUUC CAGGGGGCAU GCCUGUUUGA GCGUCAUUU

GENBANK: GENBANK: MK973013.1

Macromolecule #58: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 58 / Number of copies: 237 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Buffer: pH: 7.4
• Vitrification: Cryogen name: ETHANE

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Image recording: Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 50.0 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: OTHER / Imaging mode: OTHER / Nominal defocus max: 2.5 µm / Nominal defocus min: 1.5 µm
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

Startup model

Type of model: EMDB MAP
EMDB ID:

0220

• Final reconstruction: Resolution.type: BY AUTHOR / Resolution: 2.64 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 86343
• Initial angle assignment: Type: PROJECTION MATCHING
• Final angle assignment: Type: MAXIMUM LIKELIHOOD