[English] 日本語
![](img/lk-miru.gif)
- EMDB-33737: Cryo-EM structure of the PSI-LHCI-Lhcp supercomplex from Ostreoco... -
+
Open data
-
Basic information
Entry | ![]() | |||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Title | Cryo-EM structure of the PSI-LHCI-Lhcp supercomplex from Ostreococcus tauri | |||||||||||||||
![]() | ||||||||||||||||
![]() |
| |||||||||||||||
Function / homology | ![]() chloroplast photosystem I / photosynthesis, light harvesting / chloroplast thylakoid lumen / photosynthesis, light harvesting in photosystem I / photosystem I reaction center / photosystem I / photosystem I / photosystem II / chlorophyll binding / photosynthetic electron transport in photosystem I ...chloroplast photosystem I / photosynthesis, light harvesting / chloroplast thylakoid lumen / photosynthesis, light harvesting in photosystem I / photosystem I reaction center / photosystem I / photosystem I / photosystem II / chlorophyll binding / photosynthetic electron transport in photosystem I / chloroplast thylakoid membrane / response to light stimulus / photosynthesis / chloroplast / 4 iron, 4 sulfur cluster binding / electron transfer activity / magnesium ion binding / membrane / metal ion binding Similarity search - Function | |||||||||||||||
Biological species | ![]() | |||||||||||||||
Method | single particle reconstruction / cryo EM / Resolution: 2.94 Å | |||||||||||||||
![]() | Shan J / Sheng X / Ishii A / Watanabe A / Song C / Murata K / Minagawa J / Liu Z | |||||||||||||||
Funding support | ![]() ![]()
| |||||||||||||||
![]() | ![]() Title: The photosystem I supercomplex from a primordial green alga harbors three light-harvesting complex trimers. Authors: Asako Ishii / Jianyu Shan / Xin Sheng / Eunchul Kim / Akimasa Watanabe / Makio Yokono / Chiyo Noda / Chihong Song / Kazuyoshi Murata / Zhenfeng Liu / Jun Minagawa / ![]() ![]() Abstract: As a ubiquitous picophytoplankton in the ocean and an early-branching green alga, is a model prasinophyte species for studying the functional evolution of the light-harvesting systems in ...As a ubiquitous picophytoplankton in the ocean and an early-branching green alga, is a model prasinophyte species for studying the functional evolution of the light-harvesting systems in photosynthesis. Here, we report the structure and function of the photosystem I (PSI) supercomplex in low light conditions, where it expands its photon-absorbing capacity by assembling with the light-harvesting complexes I (LHCI) and a prasinophyte-specific light-harvesting complex (Lhcp). The architecture of the supercomplex exhibits hybrid features of the plant-type and the green algal-type PSI supercomplexes, consisting of a PSI core, an Lhca1-Lhca4-Lhca2-Lhca3 belt attached on one side and an Lhca5-Lhca6 heterodimer associated on the other side between PsaG and PsaH. Interestingly, nine Lhcp subunits, including one Lhcp1 monomer with a phosphorylated amino-terminal threonine and eight Lhcp2 monomers, oligomerize into three trimers and associate with PSI on the third side between Lhca6 and PsaK. The Lhcp1 phosphorylation and the light-harvesting capacity of PSI were subjected to reversible photoacclimation, suggesting that the formation of PSI-LHCI-Lhcp supercomplex is likely due to a phosphorylation-dependent mechanism induced by changes in light intensity. Notably, this supercomplex did not exhibit far-red peaks in the 77 K fluorescence spectra, which is possibly due to the weak coupling of the chlorophyll 603-609 pair in Lhca1-4. | |||||||||||||||
History |
|
-
Structure visualization
Supplemental images |
---|
-
Downloads & links
-EMDB archive
Map data | ![]() | 202.7 MB | ![]() | |
---|---|---|---|---|
Header (meta data) | ![]() ![]() | 48.3 KB 48.3 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 13.5 KB | Display | ![]() |
Images | ![]() | 179 KB | ||
Others | ![]() ![]() | 171.4 MB 171.4 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 1.1 MB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 1.1 MB | Display | |
Data in XML | ![]() | 20.4 KB | Display | |
Data in CIF | ![]() | 26 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7ycaMC ![]() 8hg3C ![]() 8hg5C ![]() 8hg6C M: atomic model generated by this map C: citing same article ( |
---|---|
Similar structure data | Similarity search - Function & homology ![]() |
-
Links
EMDB pages | ![]() ![]() |
---|---|
Related items in Molecule of the Month |
-
Map
File | ![]() | ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Voxel size | X=Y=Z: 1.04 Å | ||||||||||||||||||||
Density |
| ||||||||||||||||||||
Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
|
-Supplemental data
-Half map: #2
File | emd_33737_half_map_1.map | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Projections & Slices |
| ||||||||||||
Density Histograms |
-Half map: #1
File | emd_33737_half_map_2.map | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Projections & Slices |
| ||||||||||||
Density Histograms |
-
Sample components
+Entire : photosystem I-light-harvesting complex I-prasinophyte-specific Lh...
+Supramolecule #1: photosystem I-light-harvesting complex I-prasinophyte-specific Lh...
+Macromolecule #1: Lhca1
+Macromolecule #2: Chlorophyll a-b binding protein, chloroplastic
+Macromolecule #3: Chlorophyll a-b binding protein, chloroplastic
+Macromolecule #4: Chlorophyll a-b binding protein, chloroplastic
+Macromolecule #5: Chlorophyll a-b binding protein, chloroplastic
+Macromolecule #6: Lhca6
+Macromolecule #7: Photosystem I P700 chlorophyll a apoprotein A1
+Macromolecule #8: Photosystem I P700 chlorophyll a apoprotein A2
+Macromolecule #9: Photosystem I iron-sulfur center
+Macromolecule #10: Photosystem I reaction center subunit II, chloroplastic
+Macromolecule #11: Photosystem I reaction centre subunit IV
+Macromolecule #12: Photosystem I reaction center subunit III
+Macromolecule #13: PsaG
+Macromolecule #14: Photosystem I PsaH, reaction centre subunit VI
+Macromolecule #15: Photosystem I reaction center subunit VIII
+Macromolecule #16: Photosystem I reaction center subunit IX
+Macromolecule #17: Photosystem I PsaG/PsaK protein
+Macromolecule #18: PSI subunit V
+Macromolecule #19: Photosystem I reaction center subunit XII
+Macromolecule #20: Photosystem I PsaN, reaction centre subunit N
+Macromolecule #21: PsaO
+Macromolecule #22: Chlorophyll a-b binding protein, chloroplastic
+Macromolecule #23: Chlorophyll a-b binding protein, chloroplastic
+Macromolecule #24: CHLOROPHYLL B
+Macromolecule #25: CHLOROPHYLL A
+Macromolecule #26: (3~{E},5~{E},7~{E},9~{E},11~{E},13~{E},15~{E},17~{E})-1-[(1~{S},4...
+Macromolecule #27: (3S,5R,6S,3'S,5'R,6'S)-5,6,5',6'-DIEPOXY-5,6,5',6'- TETRAHYDRO-BE...
+Macromolecule #28: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE
+Macromolecule #29: (1~{S})-3,5,5-trimethyl-4-[(3~{E},5~{E},7~{E},9~{E},11~{E},13~{E}...
+Macromolecule #30: BETA-CAROTENE
+Macromolecule #31: 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE
+Macromolecule #32: 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL
+Macromolecule #33: CHLOROPHYLL A ISOMER
+Macromolecule #34: PHYLLOQUINONE
+Macromolecule #35: IRON/SULFUR CLUSTER
+Macromolecule #36: DIGALACTOSYL DIACYL GLYCEROL (DGDG)
+Macromolecule #37: (1R,3R)-6-{(3E,5E,7E,9E,11E,13E,15E,17E)-18-[(1S,4R,6R)-4-HYDROXY...
+Macromolecule #38: Chlorophyll c2
+Macromolecule #39: water
-Experimental details
-Structure determination
Method | cryo EM |
---|---|
![]() | single particle reconstruction |
Aggregation state | particle |
-
Sample preparation
Buffer | pH: 6.5 |
---|---|
Grid | Model: Quantifoil R1.2/1.3 |
Vitrification | Cryogen name: NITROGEN / Chamber humidity: 100 % / Chamber temperature: 277 K |
-
Electron microscopy
Microscope | FEI TITAN |
---|---|
Image recording | Film or detector model: GATAN K2 BASE (4k x 4k) / Average electron dose: 60.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: OTHER / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.2 µm / Nominal defocus min: 1.8 µm |
+
Image processing
-Atomic model buiding 1
Initial model | (Chain: PDB, experimental model, PDB, experimental model, PDB, experimental model, PDB, experimental model, PDB, experimental model, PDB, experimental model, PDB, experimental model, PDB, experimental model, PDB, experimental model, PDB, experimental model, PDB, experimental model, PDB, experimental model, PDB, experimental model, PDB, experimental model, PDB, experimental model) |
---|---|
Details | Initial local fitting was done using Chimera and then Wincoot was usd for flexible fitting |
Refinement | Space: REAL / Protocol: RIGID BODY FIT / Overall B value: 110.967 / Target criteria: Correlation coefficient |
Output model | ![]() PDB-7yca: |