[English] 日本語
![](img/lk-miru.gif)
- EMDB-32953: Structure of the phosphorylation-site double mutant S431E/T432E o... -
+
Open data
-
Basic information
Entry | ![]() | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Title | Structure of the phosphorylation-site double mutant S431E/T432E of the KaiC circadian clock protein | |||||||||
![]() | ||||||||||
![]() |
| |||||||||
![]() | MUTANT / CIRCADIAN CLOCK PROTEIN | |||||||||
Function / homology | ![]() regulation of phosphorelay signal transduction system / negative regulation of circadian rhythm / entrainment of circadian clock / protein serine/threonine/tyrosine kinase activity / Hydrolases; Acting on acid anhydrides; Acting on acid anhydrides to facilitate cellular and subcellular movement / circadian rhythm / non-specific serine/threonine protein kinase / phosphorylation / protein serine kinase activity / protein serine/threonine kinase activity ...regulation of phosphorelay signal transduction system / negative regulation of circadian rhythm / entrainment of circadian clock / protein serine/threonine/tyrosine kinase activity / Hydrolases; Acting on acid anhydrides; Acting on acid anhydrides to facilitate cellular and subcellular movement / circadian rhythm / non-specific serine/threonine protein kinase / phosphorylation / protein serine kinase activity / protein serine/threonine kinase activity / regulation of DNA-templated transcription / magnesium ion binding / ATP hydrolysis activity / DNA binding / ATP binding / identical protein binding Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.3 Å | |||||||||
![]() | Han X / Zhang DL / Hong L / Yu DQ / Wu ZL / Yang T / Rust MJ / Tu YH / Ouyang Q | |||||||||
Funding support | ![]()
| |||||||||
![]() | ![]() Title: Determining subunit-subunit interaction from statistics of cryo-EM images: observation of nearest-neighbor coupling in a circadian clock protein complex Authors: Han X / Zhang D / Hong L / Yu D / Wu Z / Yang T / Rust M / Tu Y / Ouyang Q | |||||||||
History |
|
-
Structure visualization
Supplemental images |
---|
-
Downloads & links
-EMDB archive
Map data | ![]() | 79.3 MB | ![]() | |
---|---|---|---|---|
Header (meta data) | ![]() ![]() | 14.5 KB 14.5 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 10.3 KB | Display | ![]() |
Images | ![]() | 71.4 KB | ||
Filedesc metadata | ![]() | 5.7 KB | ||
Others | ![]() ![]() | 76 MB 76 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 914.9 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 914.5 KB | Display | |
Data in XML | ![]() | 17.5 KB | Display | |
Data in CIF | ![]() | 22.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7x1zMC ![]() 7x1yC M: atomic model generated by this map C: citing same article ( |
---|---|
Similar structure data | Similarity search - Function & homology ![]() |
-
Links
EMDB pages | ![]() ![]() |
---|---|
Related items in Molecule of the Month |
-
Map
File | ![]() | ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Voxel size | X=Y=Z: 0.685 Å | ||||||||||||||||||||
Density |
| ||||||||||||||||||||
Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
|
-Supplemental data
-Half map: #1
File | emd_32953_half_map_1.map | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Projections & Slices |
| ||||||||||||
Density Histograms |
-Half map: #2
File | emd_32953_half_map_2.map | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Projections & Slices |
| ||||||||||||
Density Histograms |
-
Sample components
-Entire : KaiC hexamer
Entire | Name: KaiC hexamer |
---|---|
Components |
|
-Supramolecule #1: KaiC hexamer
Supramolecule | Name: KaiC hexamer / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1 |
---|---|
Source (natural) | Organism: ![]() Strain: PCC 7942 |
-Macromolecule #1: Circadian clock oscillator protein KaiC
Macromolecule | Name: Circadian clock oscillator protein KaiC / type: protein_or_peptide / ID: 1 / Number of copies: 6 / Enantiomer: LEVO |
---|---|
Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 54.347668 KDa |
Recombinant expression | Organism: ![]() ![]() |
Sequence | String: EHQAIAKMRT MIEGFDDISH GGLPIGRSTL VSGTSGTGKT LFSIQFLYNG IIEFDEPGVF VTFEETPQDI IKNARSFGWD LAKLVDEGK LFILDASPDP EGQEVVGGFD LSALIERINY AIQKYRARRV SIDSVTSVFQ QYDASSVVRR ELFRLVARLK Q IGATTVMT ...String: EHQAIAKMRT MIEGFDDISH GGLPIGRSTL VSGTSGTGKT LFSIQFLYNG IIEFDEPGVF VTFEETPQDI IKNARSFGWD LAKLVDEGK LFILDASPDP EGQEVVGGFD LSALIERINY AIQKYRARRV SIDSVTSVFQ QYDASSVVRR ELFRLVARLK Q IGATTVMT TERIEEYGPI ARYGVEEFVS DNVVILRNVL EGERRRRTLE ILKLRGTSHM KGEYPFTITD HGINIFPLGA MR LTQRSSN VRVSSGVVRL DEMCGGGFFK DSIILATGAT GTGKTLLVSR FVENACANKE RAILFAYEES RAQLLRNAYS WGM DFEEME RQNLLKIVCA YPESAGLEDH LQIIKSEIND FKPARIAIDS LSALARGVSN NAFRQFVIGV TGYAKQEEIT GLFT NTSDQ FMGAHSITDS HIEEITDTII LLQYVEIRGE MSRAINVFKM RGSWHDKAIR EFMISDKGPD IKDSFRNFER IISGS PTRI UniProtKB: Circadian clock oscillator protein KaiC |
-Macromolecule #2: ADENOSINE-5'-TRIPHOSPHATE
Macromolecule | Name: ADENOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 2 / Number of copies: 12 / Formula: ATP |
---|---|
Molecular weight | Theoretical: 507.181 Da |
Chemical component information | ![]() ChemComp-ATP: |
-Macromolecule #3: MAGNESIUM ION
Macromolecule | Name: MAGNESIUM ION / type: ligand / ID: 3 / Number of copies: 12 / Formula: MG |
---|---|
Molecular weight | Theoretical: 24.305 Da |
-Experimental details
-Structure determination
Method | cryo EM |
---|---|
![]() | single particle reconstruction |
Aggregation state | particle |
-
Sample preparation
Concentration | 0.30 mg/mL |
---|---|
Buffer | pH: 8 |
Vitrification | Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV |
-
Electron microscopy
Microscope | FEI TITAN KRIOS |
---|---|
Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 50.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 5.0 µm / Nominal defocus min: 0.4 µm |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |