+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-3265 | |||||||||
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Title | Single particle analysis of ATP-sensitive potassium channel | |||||||||
Map data | Heterooctamer complex of hamster SUR1 and mouse Kir6.2 | |||||||||
Sample |
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Keywords | potassium channel / ABC transporter / ATP-sensitive potassium channel | |||||||||
Function / homology | Function and homology information ATP sensitive Potassium channels / response to resveratrol / ATP-activated inward rectifier potassium channel activity / inward rectifying potassium channel / cell body fiber / Regulation of insulin secretion / sulfonylurea receptor activity / ventricular cardiac muscle tissue development / CAMKK-AMPK signaling cascade / ABC-family proteins mediated transport ...ATP sensitive Potassium channels / response to resveratrol / ATP-activated inward rectifier potassium channel activity / inward rectifying potassium channel / cell body fiber / Regulation of insulin secretion / sulfonylurea receptor activity / ventricular cardiac muscle tissue development / CAMKK-AMPK signaling cascade / ABC-family proteins mediated transport / voltage-gated monoatomic ion channel activity involved in regulation of presynaptic membrane potential / response to stress / Ion homeostasis / inward rectifier potassium channel activity / ATPase-coupled monoatomic cation transmembrane transporter activity / nervous system process / regulation of monoatomic ion transmembrane transport / inorganic cation transmembrane transport / ankyrin binding / response to ATP / response to testosterone / potassium ion import across plasma membrane / action potential / potassium ion binding / potassium channel activity / intercalated disc / axolemma / ABC-type transporter activity / cellular response to nutrient levels / negative regulation of insulin secretion / T-tubule / heat shock protein binding / positive regulation of protein localization to plasma membrane / potassium ion transport / acrosomal vesicle / response to ischemia / determination of adult lifespan / cellular response to glucose stimulus / sarcolemma / cellular response to nicotine / glucose metabolic process / nuclear envelope / response to estradiol / cellular response to tumor necrosis factor / presynaptic membrane / transmembrane transporter binding / endosome / response to hypoxia / response to xenobiotic stimulus / neuronal cell body / glutamatergic synapse / apoptotic process / ATP hydrolysis activity / ATP binding / plasma membrane / cytoplasm Similarity search - Function | |||||||||
Biological species | Cricetus cricetus (black-bellied hamster) / Mus musculus (house mouse) | |||||||||
Method | single particle reconstruction / negative staining / Resolution: 18.0 Å | |||||||||
Authors | Kimura Y / Kanno R / Matsuo M / Kato H / Mitsuoka K / Kazumitsu U | |||||||||
Citation | Journal: The Journal of Biological Chemistry Title: Nucleotide-dependent conformational change of the ATP-sensitive potassium channel Authors: Kimura Y / Kanno R / Matsuo M / Kato H / Mitsuoka K / Kazumitsu U | |||||||||
History |
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-Structure visualization
Movie |
Movie viewer |
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Structure viewer | EM map: SurfViewMolmilJmol/JSmol |
Supplemental images |
-Downloads & links
-EMDB archive
Map data | emd_3265.map.gz | 495.6 KB | EMDB map data format | |
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Header (meta data) | emd-3265-v30.xml emd-3265.xml | 9.7 KB 9.7 KB | Display Display | EMDB header |
Images | emd_3265.png | 31.8 KB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-3265 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-3265 | HTTPS FTP |
-Validation report
Summary document | emd_3265_validation.pdf.gz | 194.4 KB | Display | EMDB validaton report |
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Full document | emd_3265_full_validation.pdf.gz | 193.5 KB | Display | |
Data in XML | emd_3265_validation.xml.gz | 5 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-3265 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-3265 | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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Related items in Molecule of the Month |
-Map
File | Download / File: emd_3265.map.gz / Format: CCP4 / Size: 3.7 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Annotation | Heterooctamer complex of hamster SUR1 and mouse Kir6.2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 3.6 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
-Sample components
-Entire : ATP-sensitive potassium channel complex consisting of hamster SUR...
Entire | Name: ATP-sensitive potassium channel complex consisting of hamster SUR1 and mouse Kir6.2 |
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Components |
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-Supramolecule #1000: ATP-sensitive potassium channel complex consisting of hamster SUR...
Supramolecule | Name: ATP-sensitive potassium channel complex consisting of hamster SUR1 and mouse Kir6.2 type: sample / ID: 1000 Oligomeric state: Four SUR1 and four Kir6.2 forms heterooctamer Number unique components: 2 |
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Molecular weight | Theoretical: 880 KDa |
-Macromolecule #1: ATP-binding cassette sub-family C member 8
Macromolecule | Name: ATP-binding cassette sub-family C member 8 / type: protein_or_peptide / ID: 1 / Name.synonym: ABCC8, SUR1 / Number of copies: 4 / Recombinant expression: Yes |
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Source (natural) | Organism: Cricetus cricetus (black-bellied hamster) / synonym: Black-bellied hamster |
Molecular weight | Theoretical: 180 KDa |
Recombinant expression | Organism: Homo sapiens (human) / Recombinant strain: human / Recombinant cell: FreeStyle 293F / Recombinant plasmid: pCDNA3.1 |
Sequence | UniProtKB: ATP-binding cassette sub-family C member 8 |
-Macromolecule #2: ATP-sensitive inward rectifier potassium channel 11
Macromolecule | Name: ATP-sensitive inward rectifier potassium channel 11 / type: protein_or_peptide / ID: 2 / Name.synonym: KCNJ11, Kir6.2 / Number of copies: 4 / Recombinant expression: Yes |
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Source (natural) | Organism: Mus musculus (house mouse) / synonym: Mouse |
Molecular weight | Theoretical: 44 KDa |
Recombinant expression | Organism: Homo sapiens (human) / Recombinant strain: human / Recombinant cell: FreeStyle 293F / Recombinant plasmid: pCDNA3.1 |
Sequence | UniProtKB: ATP-sensitive inward rectifier potassium channel 11 |
-Experimental details
-Structure determination
Method | negative staining |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Concentration | 0.1 mg/mL |
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Buffer | pH: 7.2 Details: 50 mM HEPES-Na, 50 mM NaCl, 50 mM KCl, 5% glycerol, 1 mM 2-mercaptoethanol, 0.05% C12E8 |
Staining | Type: NEGATIVE Details: Purified protein was stained with 2% uranyl acetate for 10 seconds on a carbon-coated copper grid |
Grid | Details: 400 mesh copper grid with thin carbon support, glow discharged |
Vitrification | Cryogen name: NONE / Instrument: OTHER |
-Electron microscopy
Microscope | JEOL 1010 |
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Date | Jun 9, 2009 |
Image recording | Category: CCD / Film or detector model: OTHER / Number real images: 34 / Bits/pixel: 8 |
Tilt angle min | 0 |
Tilt angle max | 0 |
Electron beam | Acceleration voltage: 100 kV / Electron source: TUNGSTEN HAIRPIN |
Electron optics | Calibrated magnification: 38400 / Illumination mode: SPOT SCAN / Imaging mode: BRIGHT FIELD / Nominal magnification: 30000 |
Sample stage | Specimen holder model: JEOL |
-Image processing
Details | The particles were selected using an automatic selection program. |
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Final reconstruction | Applied symmetry - Point group: C4 (4 fold cyclic) / Algorithm: OTHER / Resolution.type: BY AUTHOR / Resolution: 18.0 Å / Resolution method: OTHER / Software - Name: IMAGIC / Number images used: 12117 |
Final two d classification | Number classes: 82 |