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Yorodumi- EMDB-32415: The block1 map of Getah virus using block-based reconstruction method. -
+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-32415 | |||||||||
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Title | The block1 map of Getah virus using block-based reconstruction method. | |||||||||
Map data | Cryo-EM structure of Getah virus, Block-based reconstruction (block1) | |||||||||
Sample |
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Biological species | Getah virus | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.7 Å | |||||||||
Authors | Wang M / Sun ZZ | |||||||||
Funding support | 1 items
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Citation | Journal: To Be Published Title: Implications for the pathogenicity and antigenicity of alpha viruses revealed by a 3.5 angstrom Cryo-EM structure of Getah virus Authors: Wang M / Sun ZZ / Wang JF | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_32415.map.gz | 117.1 MB | EMDB map data format | |
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Header (meta data) | emd-32415-v30.xml emd-32415.xml | 7.6 KB 7.6 KB | Display Display | EMDB header |
Images | emd_32415.png | 128.8 KB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-32415 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-32415 | HTTPS FTP |
-Validation report
Summary document | emd_32415_validation.pdf.gz | 579.6 KB | Display | EMDB validaton report |
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Full document | emd_32415_full_validation.pdf.gz | 579.1 KB | Display | |
Data in XML | emd_32415_validation.xml.gz | 6.5 KB | Display | |
Data in CIF | emd_32415_validation.cif.gz | 7.4 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-32415 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-32415 | HTTPS FTP |
-Related structure data
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_32415.map.gz / Format: CCP4 / Size: 125 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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Annotation | Cryo-EM structure of Getah virus, Block-based reconstruction (block1) | ||||||||||||||||||||||||||||||||||||
Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.36 Å | ||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Sample components
-Entire : Getah virus
Entire | Name: Getah virus |
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Components |
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-Supramolecule #1: Getah virus
Supramolecule | Name: Getah virus / type: virus / ID: 1 / Parent: 0 / Macromolecule list: #1-#2 / NCBI-ID: 59300 / Sci species name: Getah virus / Virus type: VIRION / Virus isolate: STRAIN / Virus enveloped: Yes / Virus empty: No |
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Host (natural) | Organism: Getah virus |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | cell |
-Sample preparation
Buffer | pH: 7.4 |
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Vitrification | Cryogen name: NITROGEN |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 35.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 1.0 µm |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
-Image processing
Particle selection | Number selected: 36066 / Details: region for particle picking |
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Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 3.7 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 30996 |
Initial angle assignment | Type: ANGULAR RECONSTITUTION |
Final angle assignment | Type: ANGULAR RECONSTITUTION |