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- EMDB-32261: huamn bile salt exporter ABCB11 with N-terminal truncated -

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Basic information

Entry
Database: EMDB / ID: EMD-32261
Titlehuamn bile salt exporter ABCB11 with N-terminal truncated
Map data
Sample
  • Organelle or cellular component: ABCB11
    • Protein or peptide: Human ABCB11
Biological speciesHomo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.6 Å
AuthorsWang L
Funding support China, 1 items
OrganizationGrant numberCountry
Ministry of Science and Technology (MoST, China) China
CitationJournal: Cell Res / Year: 2020
Title: Cryo-EM structure of human bile salts exporter ABCB11.
Authors: Liang Wang / Wen-Tao Hou / Li Chen / Yong-Liang Jiang / Da Xu / Linfeng Sun / Cong-Zhao Zhou / Yuxing Chen /
History
DepositionNov 23, 2021-
Header (metadata) releaseNov 30, 2022-
Map releaseNov 30, 2022-
UpdateNov 30, 2022-
Current statusNov 30, 2022Processing site: PDBj / Status: Released

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Structure visualization

Supplemental images

emd_32261.png

Downloads & links

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Map

FileDownload / File: emd_32261.map.gz / Format: CCP4 / Size: 64 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.07 Å/pix.
x 256 pix.
= 273.92 Å		1.07 Å/pix.
x 256 pix.
= 273.92 Å		1.07 Å/pix.
x 256 pix.
= 273.92 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.07 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.2
Minimum - Maximum-1.0762985 - 1.7533152
Average (Standard dev.)0.00027530876 (±0.03637838)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions256256256
Spacing256256256
CellA=B=C: 273.92 Å
α=β=γ: 90.0 °

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Supplemental data

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Sample components

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Entire : ABCB11

EntireName: ABCB11
Components
  • Organelle or cellular component: ABCB11
    • Protein or peptide: Human ABCB11

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Supramolecule #1: ABCB11

SupramoleculeName: ABCB11 / type: organelle_or_cellular_component / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Homo sapiens (human)

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Macromolecule #1: Human ABCB11

MacromoleculeName: Human ABCB11 / type: protein_or_peptide / ID: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
SequenceString: VGFFQLFRFS SSTDIWLMFV GSLCAF LHG IAQPGVLLIF GTMTDVFIDY DVELQELQIP GKACVNNTIV WTNSSLNQNM TNGTRCGLLN IESEMIKFAS YYAGIAVAVL ITGYIQICFW VIAAARQIQK MRKFYFRRIM RMEIGWFDCN SVGELNTRFS DDINKINDAI ...String:
VGFFQLFRFS SSTDIWLMFV GSLCAF LHG IAQPGVLLIF GTMTDVFIDY DVELQELQIP GKACVNNTIV WTNSSLNQNM TNGTRCGLLN IESEMIKFAS YYAGIAVAVL ITGYIQICFW VIAAARQIQK MRKFYFRRIM RMEIGWFDCN SVGELNTRFS DDINKINDAI ADQMALFIQR MTSTICGFLL GFFRGWKLTL VIISVSPLIG IGAATIGLSV SKFTDYELKA YAKAGVVADE VISSMRTVAA FGGEKREVER YEKNLVFAQR WGIRKGIVMG FFTGFVWCLI FLCYALAFWY GSTLVLDEGE YTPGTLVQIF LSVIVGALNL GNASPCLEAF ATGRAAATSI FETIDRKPII DCMSEDGYKL DRIKGEIEFH NVTFHYPSRP EVKILNDLNM VIKPGEMTAL VGPSGAGKST ALQLIQRFYD PCEGMVTVDG HDIRSLNIQW LRDQIGIVEQ EPVLFSTTIA ENIRYGREDA TMEDIVQAAK EANAYNFIMD LPQQFDTLVG EGGGQMSGGQ KQRVAIARAL IRNPKILLLD MATSALDNES EAMVQEVLSK IQHGHTIISV AHRLSTVRAA DTIIGFEHGT AVERGTHEEL LERKGVYFTL VTLQSQGNQA LNEEDIKDAT EDDMLARTFS RGSYQDSLRA SIRQRSKSQL SYLVHEPPLA VVDHKSTYEE DRKDKDIPVQ EEVEPAPVRR ILKFSAPEWP YMLVGSVGAA VNGTVTPLYA FLFSQILGTF SIPDKEEQRS QINGVCLLFV AMGCVSLFTQ FLQGYAFAKS GELLTKRLRK FGFRAMLGQD IAWFDDLRNS PGALTTRLAT DASQVQGAAG SQIGMIVNSF TNVTVAMIIA FSFSWKLSLV ILCFFPFLAL SGATQTRMLT GFASRDKQAL EMVGQITNEA LSNIRTVAGI GKERRFIEAL ETELEKPFKT AIQKANIYGF CFAFAQCIMF IANSASYRYG GYLISNEGLH FSYVFRVISA VVLSATALGR AFSYTPSYAK AKISAARFFQ LLDRQPPISV YNTAGEKWDN FQGKIDFVDC KFTYPSRPDS QVLNGLSVSI SPGQTLAFVG SSGCGKSTSI QLLERFYDPD QGKVMIDGHD SKKVNVQFLR SNIGIVSQEP VLFACSIMDN IKYGDNTKEI PMERVIAAAK QAQLHDFVMS LPEKYETNVG SQGSQLSRGE KQRIAIARAI VRDPKILLLD EATSALDTES EKTVQVALDK AREGRTCIVI AHRLSTIQNA DIIAVMAQGV VIEKGTHEEL MAQKGAYYKL VTTGSPIS

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 8
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 60.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: SPOT SCAN / Imaging mode: DIFFRACTION
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.6 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 122754
Initial angle assignmentType: RANDOM ASSIGNMENT
Final angle assignmentType: RANDOM ASSIGNMENT
FSC plot (resolution estimation)

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