[English] 日本語
Yorodumi- EMDB-31732: ACE2 -Targeting Monoclonal Antibody as Potent and Broad-Spectrum ... -
+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-31732 | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Title | ACE2 -Targeting Monoclonal Antibody as Potent and Broad-Spectrum Coronavirus Blocker | ||||||||||||
Map data | Overall structure of 3E8 bound with AEC2-B0AT1 complex | ||||||||||||
Sample |
| ||||||||||||
Function / homology | Function and homology information Defective SLC6A19 causes Hartnup disorder (HND) / Defective SLC6A19 causes Hartnup disorder (HND) / amino acid transmembrane transporter activity / Na+/Cl- dependent neurotransmitter transporters / Amino acid transport across the plasma membrane / neutral L-amino acid transmembrane transporter activity / symporter activity / amino acid transport / positive regulation of amino acid transport / angiotensin-converting enzyme 2 ...Defective SLC6A19 causes Hartnup disorder (HND) / Defective SLC6A19 causes Hartnup disorder (HND) / amino acid transmembrane transporter activity / Na+/Cl- dependent neurotransmitter transporters / Amino acid transport across the plasma membrane / neutral L-amino acid transmembrane transporter activity / symporter activity / amino acid transport / positive regulation of amino acid transport / angiotensin-converting enzyme 2 / positive regulation of L-proline import across plasma membrane / Hydrolases; Acting on peptide bonds (peptidases); Metallocarboxypeptidases / angiotensin-mediated drinking behavior / tryptophan transport / positive regulation of gap junction assembly / regulation of systemic arterial blood pressure by renin-angiotensin / regulation of vasoconstriction / regulation of cardiac conduction / peptidyl-dipeptidase activity / sodium ion transmembrane transport / angiotensin maturation / maternal process involved in female pregnancy / Metabolism of Angiotensinogen to Angiotensins / metallocarboxypeptidase activity / Attachment and Entry / negative regulation of signaling receptor activity / carboxypeptidase activity / regulation of cytokine production / positive regulation of cardiac muscle contraction / viral life cycle / blood vessel diameter maintenance / response to nutrient / negative regulation of smooth muscle cell proliferation / regulation of transmembrane transporter activity / brush border membrane / cilium / negative regulation of ERK1 and ERK2 cascade / endocytic vesicle membrane / metallopeptidase activity / positive regulation of reactive oxygen species metabolic process / virus receptor activity / regulation of cell population proliferation / regulation of inflammatory response / endopeptidase activity / Induction of Cell-Cell Fusion / Potential therapeutics for SARS / entry receptor-mediated virion attachment to host cell / receptor-mediated endocytosis of virus by host cell / Attachment and Entry / membrane fusion / receptor-mediated virion attachment to host cell / symbiont entry into host cell / membrane raft / apical plasma membrane / endoplasmic reticulum lumen / cell surface / extracellular space / extracellular exosome / zinc ion binding / extracellular region / membrane / identical protein binding / plasma membrane Similarity search - Function | ||||||||||||
Biological species | Homo sapiens (human) / Severe acute respiratory syndrome coronavirus 2 | ||||||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.2 Å | ||||||||||||
Authors | Yan RH / Zhang YY / Li YN / Zhou Q | ||||||||||||
Funding support | China, 3 items
| ||||||||||||
Citation | Journal: Science / Year: 2020 Title: Structural basis for the recognition of SARS-CoV-2 by full-length human ACE2. Authors: Renhong Yan / Yuanyuan Zhang / Yaning Li / Lu Xia / Yingying Guo / Qiang Zhou / Abstract: Angiotensin-converting enzyme 2 (ACE2) is the cellular receptor for severe acute respiratory syndrome-coronavirus (SARS-CoV) and the new coronavirus (SARS-CoV-2) that is causing the serious ...Angiotensin-converting enzyme 2 (ACE2) is the cellular receptor for severe acute respiratory syndrome-coronavirus (SARS-CoV) and the new coronavirus (SARS-CoV-2) that is causing the serious coronavirus disease 2019 (COVID-19) epidemic. Here, we present cryo-electron microscopy structures of full-length human ACE2 in the presence of the neutral amino acid transporter BAT1 with or without the receptor binding domain (RBD) of the surface spike glycoprotein (S protein) of SARS-CoV-2, both at an overall resolution of 2.9 angstroms, with a local resolution of 3.5 angstroms at the ACE2-RBD interface. The ACE2-BAT1 complex is assembled as a dimer of heterodimers, with the collectrin-like domain of ACE2 mediating homodimerization. The RBD is recognized by the extracellular peptidase domain of ACE2 mainly through polar residues. These findings provide important insights into the molecular basis for coronavirus recognition and infection. | ||||||||||||
History |
|
-Structure visualization
Supplemental images |
---|
-Downloads & links
-EMDB archive
Map data | emd_31732.map.gz | 85.5 MB | EMDB map data format | |
---|---|---|---|---|
Header (meta data) | emd-31732-v30.xml emd-31732.xml | 17.8 KB 17.8 KB | Display Display | EMDB header |
Images | emd_31732.png | 60 KB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-31732 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-31732 | HTTPS FTP |
-Related structure data
Related structure data | 7v61MC M: atomic model generated by this map C: citing same article (ref.) |
---|---|
Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
---|---|
Related items in Molecule of the Month |
-Map
File | Download / File: emd_31732.map.gz / Format: CCP4 / Size: 91.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Annotation | Overall structure of 3E8 bound with AEC2-B0AT1 complex | ||||||||||||||||||||
Voxel size | X=Y=Z: 1.077 Å | ||||||||||||||||||||
Density |
| ||||||||||||||||||||
Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
|
-Supplemental data
-Sample components
-Entire : ACE2-B0AT1 complex
Entire | Name: ACE2-B0AT1 complex |
---|---|
Components |
|
-Supramolecule #1: ACE2-B0AT1 complex
Supramolecule | Name: ACE2-B0AT1 complex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#2 |
---|---|
Source (natural) | Organism: Homo sapiens (human) |
Recombinant expression | Organism: Homo sapiens (human) |
-Macromolecule #1: Sodium-dependent neutral amino acid transporter B(0)AT1
Macromolecule | Name: Sodium-dependent neutral amino acid transporter B(0)AT1 type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO |
---|---|
Source (natural) | Organism: Homo sapiens (human) |
Molecular weight | Theoretical: 73.289359 KDa |
Recombinant expression | Organism: Homo sapiens (human) |
Sequence | String: MADYKDDDDK SGPDEVDASG RVRLVLPNPG LDARIPSLAE LETIEQEEAS SRPKWDNKAQ YMLTCLGFCV GLGNVWRFPY LCQSHGGGA FMIPFLILLV LEGIPLLYLE FAIGQRLRRG SLGVWSSIHP ALKGLGLASM LTSFMVGLYY NTIISWIMWY L FNSFQEPL ...String: MADYKDDDDK SGPDEVDASG RVRLVLPNPG LDARIPSLAE LETIEQEEAS SRPKWDNKAQ YMLTCLGFCV GLGNVWRFPY LCQSHGGGA FMIPFLILLV LEGIPLLYLE FAIGQRLRRG SLGVWSSIHP ALKGLGLASM LTSFMVGLYY NTIISWIMWY L FNSFQEPL PWSDCPLNEN QTGYVDECAR SSPVDYFWYR ETLNISTSIS DSGSIQWWML LCLACAWSVL YMCTIRGIET TG KAVYITS TLPYVVLTIF LIRGLTLKGA TNGIVFLFTP NVTELAQPDT WLDAGAQVFF SFSLAFGGLI SFSSYNSVHN NCE KDSVIV SIINGFTSVY VAIVVYSVIG FRATQRYDDC FSTNILTLIN GFDLPEGNVT QENFVDMQQR CNASDPAAYA QLVF QTCDI NAFLSEAVEG TGLAFIVFTE AITKMPLSPL WSVLFFIMLF CLGLSSMFGN MEGVVVPLQD LRVIPPKWPK EVLTG LICL GTFLIGFIFT LNSGQYWLSL LDSYAGSIPL LIIAFCEMFS VVYVYGVDRF NKDIEFMIGH KPNIFWQVTW RVVSPL LML IIFLFFFVVE VSQELTYSIW DPGYEEFPKS QKISYPNWVY VVVVIVAGVP SLTIPGYAIY KLIRNHCQKP GDHQGLV ST LSTASMNGDL KY |
-Macromolecule #2: Angiotensin-converting enzyme 2
Macromolecule | Name: Angiotensin-converting enzyme 2 / type: protein_or_peptide / ID: 2 / Number of copies: 2 / Enantiomer: LEVO / EC number: angiotensin-converting enzyme 2 |
---|---|
Source (natural) | Organism: Homo sapiens (human) |
Molecular weight | Theoretical: 93.971266 KDa |
Recombinant expression | Organism: Homo sapiens (human) |
Sequence | String: MASGRSSSSW LLLSLVAVTA AWSHPQFEKQ STIEEQAKTF LDKFNHEAED LFYQSSLASW NYNTNITEEN VQNMNNAGDK WSAFLKEQS TLAQMYPLQE IQNLTVKLQL QALQQNGSSV LSEDKSKRLN TILNTMSTIY STGKVCNPDN PQECLLLEPG L NEIMANSL ...String: MASGRSSSSW LLLSLVAVTA AWSHPQFEKQ STIEEQAKTF LDKFNHEAED LFYQSSLASW NYNTNITEEN VQNMNNAGDK WSAFLKEQS TLAQMYPLQE IQNLTVKLQL QALQQNGSSV LSEDKSKRLN TILNTMSTIY STGKVCNPDN PQECLLLEPG L NEIMANSL DYNERLWAWE SWRSEVGKQL RPLYEEYVVL KNEMARANHY EDYGDYWRGD YEVNGVDGYD YSRGQLIEDV EH TFEEIKP LYEHLHAYVR AKLMNAYPSY ISPIGCLPAH LLGDMWGRFW TNLYSLTVPF GQKPNIDVTD AMVDQAWDAQ RIF KEAEKF FVSVGLPNMT QGFWENSMLT DPGNVQKAVC HPTAWDLGKG DFRILMCTKV TMDDFLTAHH EMGHIQYDMA YAAQ PFLLR NGANEGFHEA VGEIMSLSAA TPKHLKSIGL LSPDFQEDNE TEINFLLKQA LTIVGTLPFT YMLEKWRWMV FKGEI PKDQ WMKKWWEMKR EIVGVVEPVP HDETYCDPAS LFHVSNDYSF IRYYTRTLYQ FQFQEALCQA AKHEGPLHKC DISNST EAG QKLFNMLRLG KSEPWTLALE NVVGAKNMNV RPLLNYFEPL FTWLKDQNKN SFVGWSTDWS PYADQSIKVR ISLKSAL GD KAYEWNDNEM YLFRSSVAYA MRQYFLKVKN QMILFGEEDV RVANLKPRIS FNFFVTAPKN VSDIIPRTEV EKAIRMSR S RINDAFRLND NSLEFLGIQP TLGPPNQPPV SIWLIVFGVV MGVIVVGIVI LIFTGIRDRK KKNKARSGEN PYASIDISK GENNPGFQNT DDVQTSF |
-Macromolecule #3: 3E8
Macromolecule | Name: 300000000 / type: protein_or_peptide / ID: 3 / Number of copies: 2 / Enantiomer: LEVO |
---|---|
Source (natural) | Organism: Severe acute respiratory syndrome coronavirus 2 |
Molecular weight | Theoretical: 49.96698 KDa |
Recombinant expression | Organism: Severe acute respiratory syndrome coronavirus 2 |
Sequence | String: EVQLEESGAE LVRPGASVKI SCKAFGYTFT NHHINWMKQR PGQGLDWIGY VNPYNDYTKY SQNFKGKATL SVDRSSSTAY MELSSLTSE DSAVYYCARW RDYDRDWYFD VWGAGTTVIV SSASTKGPSV FPLAPSSKST SGGTAALGCL VKDYFPEPVT V SWNSGALT ...String: EVQLEESGAE LVRPGASVKI SCKAFGYTFT NHHINWMKQR PGQGLDWIGY VNPYNDYTKY SQNFKGKATL SVDRSSSTAY MELSSLTSE DSAVYYCARW RDYDRDWYFD VWGAGTTVIV SSASTKGPSV FPLAPSSKST SGGTAALGCL VKDYFPEPVT V SWNSGALT SGVHTFPAVL QSSGLYSLSS VVTVPSSSLG TQTYICNVNH KPSNTKVDKK VEPKSCDKTH TCPPCPAPEL LG GPSVFLF PPKPKDTLMI SRTPEVTCVV VDVSHEDPEV KFNWYVDGVE VHNAKTKPRE EQYNSTYRVV SVLTVLHQDW LNG KEYKCK VSNKALPAPI EKTISKAKGQ PREPQVYTLP PSRDELTKNQ VSLTCLVKGF YPSDIAVEWE SNGQPENNYK TTPP VLDSD GSFFLYSKLT VDKSRWQQGN VFSCSVMHEA LHNHYTQKSL SLSPGK |
-Macromolecule #4: 3E8
Macromolecule | Name: 300000000 / type: protein_or_peptide / ID: 4 / Number of copies: 2 / Enantiomer: LEVO |
---|---|
Source (natural) | Organism: Severe acute respiratory syndrome coronavirus 2 |
Molecular weight | Theoretical: 23.507031 KDa |
Recombinant expression | Organism: Severe acute respiratory syndrome coronavirus 2 |
Sequence | String: DIVMTQSPAT LSVTPGDRVS LSCRASQSIR DYLYWYQQKS HESPRLLIKY ASQSISGIPS RFSGSGSGSD FTLSINSVEP EDVGVYYCQ NGHSFPYTFG GGTKLEIKRT VAAPSVFIFP PSDEQLKSGT ASVVCLLNNF YPREAKVQWK VDNALQSGNS Q ESVTEQDS ...String: DIVMTQSPAT LSVTPGDRVS LSCRASQSIR DYLYWYQQKS HESPRLLIKY ASQSISGIPS RFSGSGSGSD FTLSINSVEP EDVGVYYCQ NGHSFPYTFG GGTKLEIKRT VAAPSVFIFP PSDEQLKSGT ASVVCLLNNF YPREAKVQWK VDNALQSGNS Q ESVTEQDS KDSTYSLSST LTLSKADYEK HKVYACEVTH QGLSSPVTKS FNRGEC |
-Macromolecule #6: 2-acetamido-2-deoxy-beta-D-glucopyranose
Macromolecule | Name: 2-acetamido-2-deoxy-beta-D-glucopyranose / type: ligand / ID: 6 / Number of copies: 10 / Formula: NAG |
---|---|
Molecular weight | Theoretical: 221.208 Da |
Chemical component information | ChemComp-NAG: |
-Macromolecule #7: ZINC ION
Macromolecule | Name: ZINC ION / type: ligand / ID: 7 / Number of copies: 2 / Formula: ZN |
---|---|
Molecular weight | Theoretical: 65.409 Da |
-Macromolecule #8: water
Macromolecule | Name: water / type: ligand / ID: 8 / Number of copies: 4 / Formula: HOH |
---|---|
Molecular weight | Theoretical: 18.015 Da |
Chemical component information | ChemComp-HOH: |
-Experimental details
-Structure determination
Method | cryo EM |
---|---|
Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 8 |
---|---|
Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
---|---|
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy |
Sample stage | Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN |
Image recording | Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 50.0 e/Å2 |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
-Image processing
Initial angle assignment | Type: ANGULAR RECONSTITUTION |
---|---|
Final angle assignment | Type: MAXIMUM LIKELIHOOD |
Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 3.2 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 3.0.6) / Number images used: 101662 |