+
Open data
-
Basic information
Entry | Database: EMDB / ID: EMD-3154 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Title | Salmonella enterica wild-type bacterial flagellar motor | |||||||||
![]() | Wild-type Salmonella enterica bacteria flagellar motor | |||||||||
![]() |
| |||||||||
![]() | bacterial flagellar motor / flagellar diversity / electron cryo-tomography / Campylobacter jejuni / Vibrio fischeri / Salmonella enterica | |||||||||
Biological species | ![]() | |||||||||
Method | subtomogram averaging / cryo EM / Resolution: 69.4 Å | |||||||||
![]() | Beeby M | |||||||||
![]() | ![]() Title: Diverse high-torque bacterial flagellar motors assemble wider stator rings using a conserved protein scaffold. Authors: Morgan Beeby / Deborah A Ribardo / Caitlin A Brennan / Edward G Ruby / Grant J Jensen / David R Hendrixson / ![]() ![]() Abstract: Although it is known that diverse bacterial flagellar motors produce different torques, the mechanism underlying torque variation is unknown. To understand this difference better, we combined genetic ...Although it is known that diverse bacterial flagellar motors produce different torques, the mechanism underlying torque variation is unknown. To understand this difference better, we combined genetic analyses with electron cryo-tomography subtomogram averaging to determine in situ structures of flagellar motors that produce different torques, from Campylobacter and Vibrio species. For the first time, to our knowledge, our results unambiguously locate the torque-generating stator complexes and show that diverse high-torque motors use variants of an ancestrally related family of structures to scaffold incorporation of additional stator complexes at wider radii from the axial driveshaft than in the model enteric motor. We identify the protein components of these additional scaffold structures and elucidate their sequential assembly, demonstrating that they are required for stator-complex incorporation. These proteins are widespread, suggesting that different bacteria have tailored torques to specific environments by scaffolding alternative stator placement and number. Our results quantitatively account for different motor torques, complete the assignment of the locations of the major flagellar components, and provide crucial constraints for understanding mechanisms of torque generation and the evolution of multiprotein complexes. | |||||||||
History |
|
-
Structure visualization
Movie |
![]() |
---|---|
Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
-
Downloads & links
-EMDB archive
Map data | ![]() | 22.6 MB | ![]() | |
---|---|---|---|---|
Header (meta data) | ![]() ![]() | 8.1 KB 8.1 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 7.9 KB | Display | ![]() |
Images | ![]() ![]() | 68.3 KB 68.3 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 3150C ![]() 3155C ![]() 3156C ![]() 3157C ![]() 3158C ![]() 3159C ![]() 3160C ![]() 3161C ![]() 3162C ![]() 3813C C: citing same article ( |
---|---|
Similar structure data | |
EM raw data | ![]() Data size: 328.0 MB Data #1: Tilt-series for salmonella enterica wild-type bacterial flagellar motor [tilt series]) |
-
Links
EMDB pages | ![]() ![]() |
---|
-
Map
File | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Annotation | Wild-type Salmonella enterica bacteria flagellar motor | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 8.122 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
|
-Supplemental data
-
Sample components
-Entire : Salmonella enterica wild-type bacterial flagellar motor
Entire | Name: Salmonella enterica wild-type bacterial flagellar motor |
---|---|
Components |
|
-Supramolecule #1000: Salmonella enterica wild-type bacterial flagellar motor
Supramolecule | Name: Salmonella enterica wild-type bacterial flagellar motor type: sample / ID: 1000 / Oligomeric state: 1 / Number unique components: 1 |
---|---|
Molecular weight | Experimental: 1 MDa / Theoretical: 1 MDa |
-Supramolecule #1: Bacterial flagellar motor
Supramolecule | Name: Bacterial flagellar motor / type: organelle_or_cellular_component / ID: 1 / Number of copies: 1 / Recombinant expression: No |
---|---|
Source (natural) | Organism: ![]() |
-Experimental details
-Structure determination
Method | cryo EM |
---|---|
![]() | subtomogram averaging |
Aggregation state | particle |
-
Sample preparation
Vitrification | Cryogen name: ETHANE-PROPANE MIXTURE / Chamber humidity: 100 % / Instrument: FEI VITROBOT MARK III / Method: Variable |
---|
-
Electron microscopy
Microscope | FEI TECNAI F20 |
---|---|
Date | Jan 1, 2015 |
Image recording | Category: CCD / Film or detector model: FEI FALCON II (4k x 4k) / Average electron dose: 120 e/Å2 |
Electron beam | Acceleration voltage: 200 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.0 mm / Nominal defocus max: -4.0 µm / Nominal defocus min: -4.0 µm / Nominal magnification: 50000 |
Sample stage | Specimen holder: Nitrogen cooled Gatan 914 / Specimen holder model: SIDE ENTRY, EUCENTRIC / Tilt series - Axis1 - Min angle: -60 ° / Tilt series - Axis1 - Max angle: 60 ° |
Experimental equipment | ![]() Model: Tecnai F20 / Image courtesy: FEI Company |