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- EMDB-31393: Structure of the phycobilisome from the red alga Porphyridium pur... -

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Basic information

Entry
Database: EMDB / ID: EMD-31393
TitleStructure of the phycobilisome from the red alga Porphyridium purpureum in Middle Light
Map data
Sample
  • Complex: phycobilisome
    • Protein or peptide: x 28 types
  • Ligand: x 4 types
Keywordslight-harvesting complex / photosynthesis
Function / homology
Function and homology information


phycobilisome / chloroplast thylakoid membrane / photosynthesis / endomembrane system / lyase activity
Similarity search - Function
: / Fasciclin domain / Four repeated domains in the Fasciclin I family of proteins, present in many other contexts. / FAS1 domain / FAS1 domain superfamily / FAS1/BIgH3 domain profile. / Allophycocyanin linker chain / Phycobilisome linker protein / Allophycocyanin, beta subunit / Phycobilisome linker domain ...: / Fasciclin domain / Four repeated domains in the Fasciclin I family of proteins, present in many other contexts. / FAS1 domain / FAS1 domain superfamily / FAS1/BIgH3 domain profile. / Allophycocyanin linker chain / Phycobilisome linker protein / Allophycocyanin, beta subunit / Phycobilisome linker domain / Phycobilisome linker domain superfamily / Phycobilisome Linker polypeptide / Phycobilisome (PBS) linker domain profile. / Phycocyanin, alpha subunit / Phycocyanin, beta subunit / CpcD-like domain / CpcD/allophycocyanin linker domain / CpcD-like domain profile. / CpcD/allophycocyanin linker domain / Phycobilisome, alpha/beta subunit / Phycobilisome, alpha/beta subunit superfamily / Phycobilisome protein / Globin-like superfamily
Similarity search - Domain/homology
Phycobilisome 31.8 kDa linker polypeptide, phycoerythrin-associated, rod / Uncharacterized protein / Phycobilisome 31.8 kDa linker polypeptide, phycoerythrin-associated, rod / Phycobilisome 31.8 kDa linker polypeptide, phycoerythrin-associated, rod / R-phycoerythrin gamma chain, chloroplastic / Uncharacterized protein / Phycobilisome 7.8 kDa linker polypeptide, allophycocyanin-associated, core / R-phycoerythrin gamma chain, chloroplastic / Phycobilisome 32.1 kDa linker polypeptide, phycocyanin-associated, rod / Uncharacterized protein ...Phycobilisome 31.8 kDa linker polypeptide, phycoerythrin-associated, rod / Uncharacterized protein / Phycobilisome 31.8 kDa linker polypeptide, phycoerythrin-associated, rod / Phycobilisome 31.8 kDa linker polypeptide, phycoerythrin-associated, rod / R-phycoerythrin gamma chain, chloroplastic / Uncharacterized protein / Phycobilisome 7.8 kDa linker polypeptide, allophycocyanin-associated, core / R-phycoerythrin gamma chain, chloroplastic / Phycobilisome 32.1 kDa linker polypeptide, phycocyanin-associated, rod / Uncharacterized protein / R-phycoerythrin gamma chain, chloroplastic / R-phycoerythrin gamma chain, chloroplastic / FAS1 domain-containing protein / Phycobilisome 27.9 kDa linker polypeptide, phycoerythrin-associated, rod / Uncharacterized protein / Phycobilisome 27.9 kDa linker polypeptide, phycoerythrin-associated, rod / Phycobilisome 31.8 kDa linker polypeptide, phycoerythrin-associated, rod / FAS1 domain-containing protein / Phycoerythrin alpha subunit / B-phycoerythrin beta chain / C-phycocyanin alpha subunit / Allophycocyanin alpha subunit / Phycobilisome rod-core linker polypeptide / C-phycocyanin beta subunit / Allophycocyanin beta 18 subunit / Phycobiliprotein ApcE / Allophycocyanin gamma subunit / Allophycocyanin beta subunit
Similarity search - Component
Biological speciesPorphyridium purpureum (eukaryote)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.0 Å
AuthorsMa JF / Sui S-F
Funding support China, 2 items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC)31670745 China
National Natural Science Foundation of China (NSFC)31861143048 China
CitationJournal: Commun Biol / Year: 2023
Title: The structural basis for light acclimation in phycobilisome light harvesting systems systems in Porphyridium purpureum
Authors: Dodson EJ / Ma J / Suissa Szlejf M / Maroudas-Sklare N / Paltiel Y / Adir N / Sun S / Sui SF / Keren N
History
DepositionJun 2, 2021-
Header (metadata) releaseSep 28, 2022-
Map releaseSep 28, 2022-
UpdateSep 17, 2025-
Current statusSep 17, 2025Processing site: PDBj / Status: Released

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Structure visualization

Supplemental images

emd_31393.png

Downloads & links

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Map

FileDownload / File: emd_31393.map.gz / Format: CCP4 / Size: 744.3 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.07 Å/pix.
x 580 pix.
= 618.28 Å		1.07 Å/pix.
x 580 pix.
= 618.28 Å		1.07 Å/pix.
x 580 pix.
= 618.28 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.066 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.02
Minimum - Maximum-0.06504417 - 0.121381335
Average (Standard dev.)0.000544629 (±0.004794267)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions580580580
Spacing580580580
CellA=B=C: 618.2801 Å
α=β=γ: 90.0 °

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Supplemental data

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Sample components

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Entire : phycobilisome

EntireName: phycobilisome
Components
  • Complex: phycobilisome
    • Protein or peptide: Phycobilisome 31.8 kDa linker polypeptide, phycoerythrin-associated, rod
    • Protein or peptide: R-phycoerythrin gamma chain, chloroplastic
    • Protein or peptide: Phycobilisome rod-core linker polypeptide
    • Protein or peptide: C-phycocyanin alpha subunit
    • Protein or peptide: C-phycocyanin beta subunit
    • Protein or peptide: Phycoerythrin alpha subunit
    • Protein or peptide: B-phycoerythrin beta chain
    • Protein or peptide: Phycobilisome 31.8 kDa linker polypeptide, phycoerythrin-associated, rod
    • Protein or peptide: Phycobilisome 31.8 kDa linker polypeptide, phycoerythrin-associated, rod
    • Protein or peptide: Phycobilisome 27.9 kDa linker polypeptide, phycoerythrin-associated, rod
    • Protein or peptide: CaRSPs2
    • Protein or peptide: CaRSPs1
    • Protein or peptide: FAS1 domain-containing protein
    • Protein or peptide: R-phycoerythrin gamma chain, chloroplastic
    • Protein or peptide: Phycobilisome 32.1 kDa linker polypeptide, phycocyanin-associated, rod
    • Protein or peptide: R-phycoerythrin gamma chain, chloroplastic
    • Protein or peptide: R-phycoerythrin gamma chain, chloroplastic
    • Protein or peptide: Phycobilisome 27.9 kDa linker polypeptide, phycoerythrin-associated, rod
    • Protein or peptide: Phycobilisome 31.8 kDa linker polypeptide, phycoerythrin-associated, rod
    • Protein or peptide: Allophycocyanin alpha subunit
    • Protein or peptide: Allophycocyanin beta subunit
    • Protein or peptide: Allophycocyanin gamma subunit
    • Protein or peptide: Allophycocyanin beta 18 subunit
    • Protein or peptide: Phycobilisome 7.8 kDa linker polypeptide, allophycocyanin-associated, core
    • Protein or peptide: Phycobilisome linker polypeptide
    • Protein or peptide: Lrc4
    • Protein or peptide: LRC5
    • Protein or peptide: FAS1 domain-containing protein
  • Ligand: PHYCOERYTHROBILIN
  • Ligand: PHYCOUROBILIN
  • Ligand: PHYCOCYANOBILIN
  • Ligand: phenylmethanesulfonic acid

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Supramolecule #1: phycobilisome

SupramoleculeName: phycobilisome / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#28
Source (natural)Organism: Porphyridium purpureum (eukaryote)
Molecular weightTheoretical: 14700 kDa/nm

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Macromolecule #1: Phycobilisome 31.8 kDa linker polypeptide, phycoerythrin-associat...

MacromoleculeName: Phycobilisome 31.8 kDa linker polypeptide, phycoerythrin-associated, rod
type: protein_or_peptide / ID: 1 / Number of copies: 6 / Enantiomer: LEVO
Source (natural)Organism: Porphyridium purpureum (eukaryote)
Molecular weightTheoretical: 39.743625 KDa
SequenceString: MAAFVTGMGA APRSQMRSVS GSAVCVRSDS VRAPGALTMR LGTVSGNSSL DKLGLDKFLS ESNRAYTPRA QPGFSSEYEQ IISATYKQL FGNAYIMDSE RAEMAKQESM FRDGQLTLKD FCRALAKTEQ YKKRFFDSRP LYGAIELNFK NILGRTPDGL E HYRAKSAV ...String:
MAAFVTGMGA APRSQMRSVS GSAVCVRSDS VRAPGALTMR LGTVSGNSSL DKLGLDKFLS ESNRAYTPRA QPGFSSEYEQ IISATYKQL FGNAYIMDSE RAEMAKQESM FRDGQLTLKD FCRALAKTEQ YKKRFFDSRP LYGAIELNFK NILGRTPDGL E HYRAKSAV YDTKGYEAFV DAFFDDGEYD EVYDDYTVPF YRGYKTEANL SMAAFTHFFR MVRGSSTSDK ANPNSMQKDI PL NYYGITK TPLAVIAPGA AGTAYTESFS GTGSWQSGRA GLNAARVALG VPATANGKSF RVEVTGYTQP GFGITAGTAV GKL YKANKL SRYPRSNKSY VVGFDELTPL YQRITKNGGT IASITPL

UniProtKB: Phycobilisome 31.8 kDa linker polypeptide, phycoerythrin-associated, rod

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Macromolecule #2: R-phycoerythrin gamma chain, chloroplastic

MacromoleculeName: R-phycoerythrin gamma chain, chloroplastic / type: protein_or_peptide / ID: 2 / Number of copies: 6 / Enantiomer: LEVO
Source (natural)Organism: Porphyridium purpureum (eukaryote)
Molecular weightTheoretical: 31.792982 KDa
SequenceString: MAAFVSGFHG VQVGAPAENK LVCRAAKPAQ LTMLTGYDSK SSPNFPNRAA TRERRTVSFN ARVARNKSQA KKILEKADEF FARSVTMQY KAFACPNGVY DIQCTEGTVK GAAYEKRAMA VSAAFRAKQA SPAAKARALF ENRRHAIIAS HECQHEEDLF V RFPKLSAA ...String:
MAAFVSGFHG VQVGAPAENK LVCRAAKPAQ LTMLTGYDSK SSPNFPNRAA TRERRTVSFN ARVARNKSQA KKILEKADEF FARSVTMQY KAFACPNGVY DIQCTEGTVK GAAYEKRAMA VSAAFRAKQA SPAAKARALF ENRRHAIIAS HECQHEEDLF V RFPKLSAA YMMGKTEAMR TCSRYVVPDS LEEEYMAASV DRQMKERACP GGVYASSCVE GNAKGQAEQA RVAALATAFR SA QKSASKT TAERYSSAAY GRDHFAHGCS YEESVFNTYP ATAAAMRSKS YNY

UniProtKB: R-phycoerythrin gamma chain, chloroplastic

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Macromolecule #3: Phycobilisome rod-core linker polypeptide

MacromoleculeName: Phycobilisome rod-core linker polypeptide / type: protein_or_peptide / ID: 3 / Number of copies: 6 / Enantiomer: LEVO
Source (natural)Organism: Porphyridium purpureum (eukaryote)
Molecular weightTheoretical: 26.996689 KDa
SequenceString: MSIPILKYSL STQNQRVESF EVNPGDEQYR IYSTDSLPSS SEVDQIIWAA YRQIFSEHQI LKSTKQPFLE SQLRFNQISI KDFIKGLVL SAPFRDLNYN VNNNYRFVEI CVQRVLGRDI YNEREKIAWS IIIATQGLEG FIDTLLNTQE YLSNFGDSIV P FQRRRIIP ...String:
MSIPILKYSL STQNQRVESF EVNPGDEQYR IYSTDSLPSS SEVDQIIWAA YRQIFSEHQI LKSTKQPFLE SQLRFNQISI KDFIKGLVL SAPFRDLNYN VNNNYRFVEI CVQRVLGRDI YNEREKIAWS IIIATQGLEG FIDTLLNTQE YLSNFGDSIV P FQRRRIIP QRSKGEMPFN LKTPRYGIEF RSKLAATQFS WASPIRRFRP QEQQPKAGDP ALFLNMVQEM GPGL

UniProtKB: Phycobilisome rod-core linker polypeptide

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Macromolecule #4: C-phycocyanin alpha subunit

MacromoleculeName: C-phycocyanin alpha subunit / type: protein_or_peptide / ID: 4 / Number of copies: 36 / Enantiomer: LEVO
Source (natural)Organism: Porphyridium purpureum (eukaryote)
Molecular weightTheoretical: 17.478664 KDa
SequenceString:
MKTPITEAIA TADNQGRFLS NTELQAVNGR YQRATASLTA AKALTGSAQR LITGAAQAVY NKFPYTTQMP GPAYASSAIG KAKCARDIG YYLRMVTYCL VVGGTGPMDE YLVAGLEEIN RSFDLSPSWY IEALQYIKNS HGLSGQVANE ANAYIDYAIN T LS

UniProtKB: C-phycocyanin alpha subunit

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Macromolecule #5: C-phycocyanin beta subunit

MacromoleculeName: C-phycocyanin beta subunit / type: protein_or_peptide / ID: 5 / Number of copies: 36 / Enantiomer: LEVO
Source (natural)Organism: Porphyridium purpureum (eukaryote)
Molecular weightTheoretical: 18.242705 KDa
SequenceString:
MLDAFAKVVA QADARGEFLS NTQIDALSKM VKEGNQRLDI VNKVTSNASA IVTNSARALF AEQPQLIQPG G(MEN)AYTS RRM AACLRDMEIV LRYVSYAMIA GDSSVLDDRC LNGLRETYQA LGTPGSSVAV AIQKMKDASV ALANDTTGTP IGDCSSL VA ELAGYFDRAA VSVV

UniProtKB: C-phycocyanin beta subunit

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Macromolecule #6: Phycoerythrin alpha subunit

MacromoleculeName: Phycoerythrin alpha subunit / type: protein_or_peptide / ID: 6 / Number of copies: 230 / Enantiomer: LEVO
Source (natural)Organism: Porphyridium purpureum (eukaryote)
Molecular weightTheoretical: 17.824029 KDa
SequenceString:
MKSVITTVVS AADAAGRFPS NSDLESIQGN IQRSAARLEA AEKLAGNHEA VVKEAGDACF AKYAYLKNPG EAGENQEKIN KCYRDVDHY MRLVNYCLVV GGTGPLDEWG IAGAREVYRT LNLPTSAYVA SIAYTRDRLC VPRDMSAQAG VEFSAYLDYL I NALS

UniProtKB: Phycoerythrin alpha subunit

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Macromolecule #7: B-phycoerythrin beta chain

MacromoleculeName: B-phycoerythrin beta chain / type: protein_or_peptide / ID: 7 / Number of copies: 250 / Enantiomer: LEVO
Source (natural)Organism: Porphyridium purpureum (eukaryote)
Molecular weightTheoretical: 18.584182 KDa
SequenceString:
MLDAFSRVVV NSDAKAAYVG GSDLQALKSF IADGNKRLDA VNSIVSNASC MVSDAVSGMI CENPGLISPG G(MEN)CYTN RRM AACLRDGEII LRYVSYALLA GDASVLEDRC LNGLKETYIA LGVPTNSSIR AVSIMKAQAV AFITNTATER KMSFAAG DC TSLASEVASY FDRVGAAIS

UniProtKB: B-phycoerythrin beta chain

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Macromolecule #8: Phycobilisome 31.8 kDa linker polypeptide, phycoerythrin-associat...

MacromoleculeName: Phycobilisome 31.8 kDa linker polypeptide, phycoerythrin-associated, rod
type: protein_or_peptide / ID: 8 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Porphyridium purpureum (eukaryote)
Molecular weightTheoretical: 32.886242 KDa
SequenceString: MEAVGFVHVG AWRVGSSSAA AAAAGVAGKR CGALPVSFGA HAPRRAASAS QVKMMAFSSI QNVLTDAPLG TPSAMGFLDE RTEKPLMGP SEMDELVYAV YKQVFGNAHI MESERAELRD AESLFRYLRD VREFVRALAK SNAYTSRFLQ GNQQYRFFEL N FKHLLGRG ...String:
MEAVGFVHVG AWRVGSSSAA AAAAGVAGKR CGALPVSFGA HAPRRAASAS QVKMMAFSSI QNVLTDAPLG TPSAMGFLDE RTEKPLMGP SEMDELVYAV YKQVFGNAHI MESERAELRD AESLFRYLRD VREFVRALAK SNAYTSRFLQ GNQQYRFFEL N FKHLLGRG PRSLEEMASH SALYAEGGFE AVIDAILDSD EYAQEFGSTV VPYCRFKGEY PTNEEFNRML VLKGANSTSD KA GKGRSKL CYSIAAGCSQ NWLSMSKALP FGTEKGTGFN LISSSRNPAA RPRIGTKIPG GVVFSS

UniProtKB: Phycobilisome 31.8 kDa linker polypeptide, phycoerythrin-associated, rod

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Macromolecule #9: Phycobilisome 31.8 kDa linker polypeptide, phycoerythrin-associat...

MacromoleculeName: Phycobilisome 31.8 kDa linker polypeptide, phycoerythrin-associated, rod
type: protein_or_peptide / ID: 9 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Porphyridium purpureum (eukaryote)
Molecular weightTheoretical: 43.968605 KDa
SequenceString: MAAFISSFAG AQLAHARPSK QTALRAALTL PGTAETNLAV PSNAVRKVQP YPIAKPPSYS SVDSLRPARV SRMDADWATI YEQVRRQVM GNAYVMEGEA PDIDVAFSQL KGGNLTVREF VRAVGKSASY RTRFMEAKSS YNFVLLNFKH FLGRAPTQEE V STHIQILA ...String:
MAAFISSFAG AQLAHARPSK QTALRAALTL PGTAETNLAV PSNAVRKVQP YPIAKPPSYS SVDSLRPARV SRMDADWATI YEQVRRQVM GNAYVMEGEA PDIDVAFSQL KGGNLTVREF VRAVGKSASY RTRFMEAKSS YNFVLLNFKH FLGRAPTQEE V STHIQILA TSGLEAEIDS YIDSDEYKAL FGDHVVPYVV YRGTYLSSER FNRMVKANPG GATSDKAKSN LNMIATVAAD LP TDAIDVM RGLPSPITSE TLAFGTAYYW AKVEKEASEG RSASPIGEKI GKFDHAPIST YTSLCSYDKV NKAPQISVTN VGS DEHSYV SVTSKYIAPD MAAAAQMLAD CQKYKAGGNA PTGKWMKYYP GTTVNMAPYI SLNDTGSDSS RTVSVTLDKV KISP NKIGK

UniProtKB: Phycobilisome 31.8 kDa linker polypeptide, phycoerythrin-associated, rod

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Macromolecule #10: Phycobilisome 27.9 kDa linker polypeptide, phycoerythrin-associat...

MacromoleculeName: Phycobilisome 27.9 kDa linker polypeptide, phycoerythrin-associated, rod
type: protein_or_peptide / ID: 10 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Porphyridium purpureum (eukaryote)
Molecular weightTheoretical: 37.175957 KDa
SequenceString: MAFVTGGVGA VVRPGTAVSR GVVCARQQAR PRAAALQMGV SDVSITLSGV AASQPVSAPA KMSLEDRQLL VLQAIKQVFG NAYVMEEER ASFAKQESMF LSGELSVREF VRELALSDTY RRRFFEPCGP YRFVELNMKH LLGRGPISQA EVSQHVQCYV N NGYEAEIS ...String:
MAFVTGGVGA VVRPGTAVSR GVVCARQQAR PRAAALQMGV SDVSITLSGV AASQPVSAPA KMSLEDRQLL VLQAIKQVFG NAYVMEEER ASFAKQESMF LSGELSVREF VRELALSDTY RRRFFEPCGP YRFVELNMKH LLGRGPISQA EVSQHVQCYV N NGYEAEIS SYVDSDEYYE RFGEDTVPYE QFRGTYMTAE DFNRMVSMYG APGQSDKSLT SRARSTGVAN SNKVLSLEGA GR SSKTVGR VATNTASSLT SVKSGIPPRP DIDQPRGQSS KRLVGRRLEI VPGSYMYLSP AEAAEYRAQQ AAVSQVSAAF SAD VQSKMA QVSKLKRELA ALGLSV

UniProtKB: Phycobilisome 27.9 kDa linker polypeptide, phycoerythrin-associated, rod

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Macromolecule #11: CaRSPs2

MacromoleculeName: CaRSPs2 / type: protein_or_peptide / ID: 11 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Porphyridium purpureum (eukaryote)
Molecular weightTheoretical: 36.402004 KDa
SequenceString: MWEQQRPRRC EAPAAPSSRP AERRAAARRS RAQLRMKQDD YEQWKTEFAG GFPGGEAFYK KWIEEGAKGD VPALEEELQP RSPNKKPTI YEEQMISNRG QQKGVDPTWK TLLAGGFPGG EFFFKKWIGE GAQGEVPNLD ADLQPGSGSA KKTGKKEDAD K SSPGGIMT ...String:
MWEQQRPRRC EAPAAPSSRP AERRAAARRS RAQLRMKQDD YEQWKTEFAG GFPGGEAFYK KWIEEGAKGD VPALEEELQP RSPNKKPTI YEEQMISNRG QQKGVDPTWK TLLAGGFPGG EFFFKKWIGE GAQGEVPNLD ADLQPGSGSA KKTGKKEDAD K SSPGGIMT PGRIMVPSGL GEEEETVDKE APRPELYNKY FSADRLHKAP EILFEYNKTK YDRVGVRYTE VTSKASERFF PK SRMNRAP VIEISYREGA VSTASVSLSM PEISGPPALP FPVPKGDVTT TMVTDPTTGR LKLEFKVDGA AVSAYSDPRA TEA YNKMIR K

UniProtKB: Uncharacterized protein

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Macromolecule #12: CaRSPs1

MacromoleculeName: CaRSPs1 / type: protein_or_peptide / ID: 12 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Porphyridium purpureum (eukaryote)
Molecular weightTheoretical: 31.172643 KDa
SequenceString: MAAFVSGGCG VGGQRRAWPA KGAAVARTHA CPTTMVVLPV ARAGLAATAK KNQYMGTSVA PEIVLTDKGS DMSRKVKTED KKVAADQAA AMGILANMSL YASLNPVKRM TYKAKEQAPA YVKKTGNPVE DFYPSSWRNM APVISLSANR VAVAFEKIDA A SNGVKANS ...String:
MAAFVSGGCG VGGQRRAWPA KGAAVARTHA CPTTMVVLPV ARAGLAATAK KNQYMGTSVA PEIVLTDKGS DMSRKVKTED KKVAADQAA AMGILANMSL YASLNPVKRM TYKAKEQAPA YVKKTGNPVE DFYPSSWRNM APVISLSANR VAVAFEKIDA A SNGVKANS NNKPFWKSGA TTKNYVAPEA PAQSEPETLD DAAYQRYFPA RIRNKAPAME FRRPSFANTE DPSAYFMLQK ET VPLRMAL AEKLLTKLGR KGDGSEAEEE KVKPQKKAAK KDAKDDAKDD E

UniProtKB: Uncharacterized protein

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Macromolecule #13: FAS1 domain-containing protein

MacromoleculeName: FAS1 domain-containing protein / type: protein_or_peptide / ID: 13 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Porphyridium purpureum (eukaryote)
Molecular weightTheoretical: 31.332955 KDa
SequenceString: MAAKPYESGY IAEDDFWRGR GIAAWVYATG ANKVIAQIVK DFNLTDKKFM VFIPNDGAFA RLSPQLRKAM MEDSRLVYDM LAGHIFTSK GSAMLKDLQG AGYLQPAYGE AIGYVGTGRV IKIGNAQVIP ESSDILRKNL GFSAHTLDTF IVPKALTKKV S IEAGFSPV ...String:
MAAKPYESGY IAEDDFWRGR GIAAWVYATG ANKVIAQIVK DFNLTDKKFM VFIPNDGAFA RLSPQLRKAM MEDSRLVYDM LAGHIFTSK GSAMLKDLQG AGYLQPAYGE AIGYVGTGRV IKIGNAQVIP ESSDILRKNL GFSAHTLDTF IVPKALTKKV S IEAGFSPV TPAKYVSTTK ADLRYVGATK PAAVGGRRAM NLMKQQPFWM YGPPYNAVTQ DEYEPISAAA PKAFVDYQIF AP GTVKVSP DSVNANELNP VSGMSKYIGK TQKLVGDQGI SDRSDKLPMG Q

UniProtKB: FAS1 domain-containing protein

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Macromolecule #14: R-phycoerythrin gamma chain, chloroplastic

MacromoleculeName: R-phycoerythrin gamma chain, chloroplastic / type: protein_or_peptide / ID: 14 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Porphyridium purpureum (eukaryote)
Molecular weightTheoretical: 36.924992 KDa
SequenceString: MAAFVVGAQV SSGFMGCAAP RKEQTRVGGA AKAAVSMRII IQDKNSYRKY QTNTSTSKWD KLLNTKPMKR QVQPNPPTNE TRALNLGNT FRSPAFKFLG TLKRSKDPSG LRLGFYGRKA DDFMARSIAM QAKASAAGSG VYTTQCSEGA SKGMAENART A SLAKQFRQ ...String:
MAAFVVGAQV SSGFMGCAAP RKEQTRVGGA AKAAVSMRII IQDKNSYRKY QTNTSTSKWD KLLNTKPMKR QVQPNPPTNE TRALNLGNT FRSPAFKFLG TLKRSKDPSG LRLGFYGRKA DDFMARSIAM QAKASAAGSG VYTTQCSEGA SKGMAENART A SLAKQFRQ AQRSAREMSF DYYEGRKYAM KAVGHICNYE EKIFQQYNKT AAAYVMGKQE TLLSCDRYAQ PANKAEEYIQ KS VQMQMKK RSIPYGVYTT SCADGTVKGM AENARVAKES ANFRARQMSA GAKAAARFNA RRVANDWHNN GCNYEEKLTS RFP AAASSV RPTTNRY

UniProtKB: R-phycoerythrin gamma chain, chloroplastic

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Macromolecule #15: Phycobilisome 32.1 kDa linker polypeptide, phycocyanin-associated, rod

MacromoleculeName: Phycobilisome 32.1 kDa linker polypeptide, phycocyanin-associated, rod
type: protein_or_peptide / ID: 15 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Porphyridium purpureum (eukaryote)
Molecular weightTheoretical: 53.601336 KDa
SequenceString: MAFVNGFSGG AAAVPRRGVA RVCAQRVVMK VVDRLDSQPS AAFEQTKQVY TFSRYILGPH RAVVAPVAMD PSEKEVVLRA VYRQVFGNA YIMEEERAEL RVMESQFLLG ELSVKELVRA LAKSSTYKVR FFEGAVQYRF IELCFKHLLG RAPDNHEEIA V HMRKYQQE ...String:
MAFVNGFSGG AAAVPRRGVA RVCAQRVVMK VVDRLDSQPS AAFEQTKQVY TFSRYILGPH RAVVAPVAMD PSEKEVVLRA VYRQVFGNA YIMEEERAEL RVMESQFLLG ELSVKELVRA LAKSSTYKVR FFEGAVQYRF IELCFKHLLG RAPDNHEEIA V HMRKYQQE GYDAEIDSYL DAGEYDNVFG DDTVPFLRFR GVYTPCDSFN RQCALQGGWA NSDKAMGGAA LSGYNGSDGR QM STMIGNY ISGKPIPYEK VAADTPLKST APNWYARPNP ALAPQPAYVS AKEIAELRSR VSKLEAAWSV AVKQSAAAKD TVE TWRAAA KEMAAMRGIS PMGEAYFGGI AQKVDNGALA QLGNKASSYK KYLYAIETDE VSRLEVDLEE AKGQLRVLEA AMAK STPMT RTAEFKTLTK NVAAVTAAEK ADPLSKRPRI SASRPKPTPA AAKMAAAEKK KGPFGLSVPS LPNLPNLPKL PSLSL PKVS LPFGKK

UniProtKB: Phycobilisome 32.1 kDa linker polypeptide, phycocyanin-associated, rod

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Macromolecule #16: R-phycoerythrin gamma chain, chloroplastic

MacromoleculeName: R-phycoerythrin gamma chain, chloroplastic / type: protein_or_peptide / ID: 16 / Number of copies: 6 / Enantiomer: LEVO
Source (natural)Organism: Porphyridium purpureum (eukaryote)
Molecular weightTheoretical: 29.453639 KDa
SequenceString: MAAFVGSAAS AFTGASAVKA NEKRSVCSLQ MVAMPQTGLV NSKFSARMAK KTAKQTKNKV DEYMARSVQR QYKQAAVATG VYGTQCTEG TVKGAAEASR SAALSRQFRI KQRSAFSKAH DLFEFRKHAI IAAAGCSYEE KMVTRFPKLA AAMVLGQTEM M RTCSRYVV ...String:
MAAFVGSAAS AFTGASAVKA NEKRSVCSLQ MVAMPQTGLV NSKFSARMAK KTAKQTKNKV DEYMARSVQR QYKQAAVATG VYGTQCTEG TVKGAAEASR SAALSRQFRI KQRSAFSKAH DLFEFRKHAI IAAAGCSYEE KMVTRFPKLA AAMVLGQTEM M RTCSRYVV PESVEEEYMA ASVDKQMKRR GAPGGVYSLS CAEGVAKGQA EIARVSALGA AYRAASKSAS AVTAERYNSM AY GRVHFAH GCSYEEQQFN KYPAAAAAMR SDSYGY

UniProtKB: R-phycoerythrin gamma chain, chloroplastic

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Macromolecule #17: R-phycoerythrin gamma chain, chloroplastic

MacromoleculeName: R-phycoerythrin gamma chain, chloroplastic / type: protein_or_peptide / ID: 17 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Porphyridium purpureum (eukaryote)
Molecular weightTheoretical: 31.358787 KDa
SequenceString: METAFVSGFM GKAAVAKFGA TAVCDKTARR SSSSNSQVHM VTGAVSSVNM RRFQRVPKVS GFSAKVTKKN VNKALDKADM FFAKSVTME GKAAAIPYGV YGIQCMEGSA KGMAHEKRAM ALSAAFRMNQ RSAAEKTGAM YENRRLALIL AQNDHQEKQY I KYPKLAAA ...String:
METAFVSGFM GKAAVAKFGA TAVCDKTARR SSSSNSQVHM VTGAVSSVNM RRFQRVPKVS GFSAKVTKKN VNKALDKADM FFAKSVTME GKAAAIPYGV YGIQCMEGSA KGMAHEKRAM ALSAAFRMNQ RSAAEKTGAM YENRRLALIL AQNDHQEKQY I KYPKLAAA ALMASTEVTR ACQRYAVPES IEEEFLAASV DKVNKMRGTT ASGVYKSSCV EGNAKGQAEQ ARVAALAVAF RS AQKSASQ FAAERYAQSK YGRDLFSSTH FEEGYANTYP AMAAAKRASS YGY

UniProtKB: R-phycoerythrin gamma chain, chloroplastic

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Macromolecule #18: Phycobilisome 27.9 kDa linker polypeptide, phycoerythrin-associat...

MacromoleculeName: Phycobilisome 27.9 kDa linker polypeptide, phycoerythrin-associated, rod
type: protein_or_peptide / ID: 18 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Porphyridium purpureum (eukaryote)
Molecular weightTheoretical: 34.958746 KDa
SequenceString: MAFVSGAGAA VRGTSGKAAA RCAAVRTVRA ARAIRMTTAP GSAPFSSLDE PERQVVMWES VGDEKDQVFK QLYRQVFGNA YLMESDLEE LLVPESQLLM GSISVKDFIK RVAKSDAYKK RFFEPCGPYR FVELCTKHFL GRGPRDQKEV SEHVQRLANE G YDADVDSY ...String:
MAFVSGAGAA VRGTSGKAAA RCAAVRTVRA ARAIRMTTAP GSAPFSSLDE PERQVVMWES VGDEKDQVFK QLYRQVFGNA YLMESDLEE LLVPESQLLM GSISVKDFIK RVAKSDAYKK RFFEPCGPYR FVELCTKHFL GRGPRDQKEV SEHVQRLANE G YDADVDSY MDSEEYMSLF GENGVPRFVF KGTYEGNDQF NRLAAMRQFA DGSYTDTRSG STAPRKAQKA ELTMAEGDFV GR AKVSRGL PAETSAAKTG TPPVRALKGP VNPRAGVRVR IKVVDNLYQV YEIPPMADPK AKVNAFWAKP IPSSLTKKY

UniProtKB: Phycobilisome 27.9 kDa linker polypeptide, phycoerythrin-associated, rod

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Macromolecule #19: Phycobilisome 31.8 kDa linker polypeptide, phycoerythrin-associat...

MacromoleculeName: Phycobilisome 31.8 kDa linker polypeptide, phycoerythrin-associated, rod
type: protein_or_peptide / ID: 19 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Porphyridium purpureum (eukaryote)
Molecular weightTheoretical: 55.146727 KDa
SequenceString: MAFVGAMPGG GGEPRAVRMM MQTDARSLSS GSSAAVPFST AVRFESPSGG LDRYSRVDPA APGPNVITRF LFKDRPVRRS DPSLSEVDR EATMRTVYRN VMGNAYVMEE ERAELATLES QFLVGAISTR DFVRGVAKSA TYKKRFFESV SQFRFIELNF K HFMGRAPL ...String:
MAFVGAMPGG GGEPRAVRMM MQTDARSLSS GSSAAVPFST AVRFESPSGG LDRYSRVDPA APGPNVITRF LFKDRPVRRS DPSLSEVDR EATMRTVYRN VMGNAYVMEE ERAELATLES QFLVGAISTR DFVRGVAKSA TYKKRFFESV SQFRFIELNF K HFMGRAPL DMAEMSKHYE IFAAGGYDAE VDSYFDSEEY LDVFGLDTVP YMRFRGTYAP NSTFNLQCRL QGGWARSDKK LP MMSMLPL NNKAAIMPHQ IVDGLPVIPN SEHPSQKYNV PKVSREKLQR ELLIAQGKAN ALQIELDAAY TSLASSRAFL APF AAMAAD MDIRPLYGKN PQVFAGQFLG VGAGQWGKTG ADTVRGRSRR VAADIGVKEF QLERVKQLVV DLQRALALED AEAD APATS LLQAYQAKVY VKPPVIAKKK GPEPVNEDEI TIGQGDKKIK VTVLRNLGDR TEKLREKPEK EEEEGPRTFK DLYET AKPM KGFPGDGSEM NVGG

UniProtKB: Phycobilisome 31.8 kDa linker polypeptide, phycoerythrin-associated, rod

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Macromolecule #20: Allophycocyanin alpha subunit

MacromoleculeName: Allophycocyanin alpha subunit / type: protein_or_peptide / ID: 20 / Number of copies: 20 / Enantiomer: LEVO
Source (natural)Organism: Porphyridium purpureum (eukaryote)
Molecular weightTheoretical: 17.639123 KDa
SequenceString:
MSIVTKSIVN ADAEARYLSP GELDRIKSFV LSGQRRLRIA QTLTENRERI VKQGGQQLFQ RRPDVVSPGG NAYGEEMTAT CLRDLDYYL RLVTYGIIAG DVTPIEEIGL VGVKEMYSAL GTPISGVAEG IRCMKDVACS LLSGEDAAEV GFYFDYTLAA M Q

UniProtKB: Allophycocyanin alpha subunit

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Macromolecule #21: Allophycocyanin beta subunit

MacromoleculeName: Allophycocyanin beta subunit / type: protein_or_peptide / ID: 21 / Number of copies: 22 / Enantiomer: LEVO
Source (natural)Organism: Porphyridium purpureum (eukaryote)
Molecular weightTheoretical: 17.415797 KDa
SequenceString:
MQDAITSVIN AADVQGKYLD ANSVEKLRGY FQTGELRVRA AATIAANAAT IIKEAVAKSL LYSDITRPGG (MEN)MYTTR RYA ACIRDLDYYL RYATYGMLAG DPSILDERVL NGLKETYNSL GVPIGATIQA IQAMKEVTGS LVGSDAGKEM GLYFDYI CS GLS

UniProtKB: Allophycocyanin beta subunit

+
Macromolecule #22: Allophycocyanin gamma subunit

MacromoleculeName: Allophycocyanin gamma subunit / type: protein_or_peptide / ID: 22 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Porphyridium purpureum (eukaryote)
Molecular weightTheoretical: 18.126934 KDa
SequenceString:
MSLVTQVILS ADDELRYPTA GELETISSYL KTGEYRIRLI SILQGKEQEI IRLASKKIFQ LHPEYIAPGG NASGARQRAL CLRDYGWYL RLITYAILAG DKEPLEKIGI IGVREMYNSL GVPIIGMIDA IKCLKEATVE VISQEEEDFV APYYDYIIQG M S

UniProtKB: Allophycocyanin gamma subunit

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Macromolecule #23: Allophycocyanin beta 18 subunit

MacromoleculeName: Allophycocyanin beta 18 subunit / type: protein_or_peptide / ID: 23 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Porphyridium purpureum (eukaryote)
Molecular weightTheoretical: 19.77874 KDa
SequenceString:
MQDIITSVIN QYDLSGRYLD IKGINQINNY FDTGLKRLMI AKLINKEATN LIKEASELLF IEQPELLRPC GNAFIGNAYT TRRYAACIR DIEYYLRYSA YSIIAGNNSI LDERVLDGLK ETYNSLLVPI GPTIRVIQLL KEIIQKKFST ENIENAIIAE P FDYLAINL SDKNL

UniProtKB: Allophycocyanin beta 18 subunit

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Macromolecule #24: Phycobilisome 7.8 kDa linker polypeptide, allophycocyanin-associa...

MacromoleculeName: Phycobilisome 7.8 kDa linker polypeptide, allophycocyanin-associated, core
type: protein_or_peptide / ID: 24 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Porphyridium purpureum (eukaryote)
Molecular weightTheoretical: 14.108206 KDa
SequenceString:
MAGGAAFVLT SSGFAGTRLR SAAVCGAQGK SRAHGVAVAM AYDMDGTGAG SLEPRGIKSS AVEKDSRCFK VTFVLPSQSR LYTFRELQN VYTTKMVPFS SWYAEQQRIQ KMGGKILKVE LVAGGQMRSV GNQ

UniProtKB: Phycobilisome 7.8 kDa linker polypeptide, allophycocyanin-associated, core

+
Macromolecule #25: Phycobilisome linker polypeptide

MacromoleculeName: Phycobilisome linker polypeptide / type: protein_or_peptide / ID: 25 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Porphyridium purpureum (eukaryote)
Molecular weightTheoretical: 99.468367 KDa
SequenceString: MVTKASGGSP VVKPQLYKTA SMLTIAQAEQ QDRFLELGEL NQLVSFLNTG NIRLEIADLL TKNANIIVAR AADRIFVGGS AISYLERPQ ASIIEANSAD IASIRQMSGD SQSNFLENLT SVFQGNNATP TGFKPISVVR YGPSRMKKSL RDLDWFLRYL T YAIVAGDP ...String:
MVTKASGGSP VVKPQLYKTA SMLTIAQAEQ QDRFLELGEL NQLVSFLNTG NIRLEIADLL TKNANIIVAR AADRIFVGGS AISYLERPQ ASIIEANSAD IASIRQMSGD SQSNFLENLT SVFQGNNATP TGFKPISVVR YGPSRMKKSL RDLDWFLRYL T YAIVAGDP NILFVNIRGL REIIENACSS AATIVALKEM KKTSLSLFPE NSIQKEIIEE YFNVVVDEFI NPALTDTIRK RT SNDLQGL RLPQIYAKAG ISRQKFVMKP GLSTDEKQSV ISACYRQVFE RDISKAYGFS FSVLESQVKN GQISIKEFVR SLG KSSVYQ KQFYQPYVNS RVVELAFRHF LGRNLSSLAE FQKFFAILSK KGLTGLVDSL INSREYSDYF NEETVPYIRG FGEE PQECR NWGTQIDLFQ YSAPFRKVPQ SITLFSDYLK ALPDQHPYGR GNDPLLIQFG AIFPIGTKNL KQNPAPFGKD TRRLL IRRG PGIYNQVGNP STRSVSVGSL GPKVFKSEGI NSNAQRTNNE SILQASYLAV FGRMIYQNER IGLKGIDNKF LDNNLS VKE LIRSLAISDT FRSLYWTPLY VCKSIEWIHY RLLGRPTYGR QEINQYFNVA YKKGFVGVIN SIIDSVEYNE CFGDNIV PY ERYLTANSVS QRQLKLGNII KSANLKPQNI EKFVQLGQSQ TNQNLYSIKY KVKQGVSKLR DQQKIFETKG SLSKDAYL S IFQAACRQIF ERDISTFVIG NEIENIKIQF IKGQISVKEM INALGKSSVY LKEFYNPYPN IKVIELGTKH FLGRAPNNQ AEIRFYNQIL ASCGLQAFID MLTNSQEYAE IFGEVRVPFR RFPTLPAANF PNTNTLFDKQ TKQNSVVIVP SFKAITGNQ

UniProtKB: Phycobiliprotein ApcE

+
Macromolecule #26: Lrc4

MacromoleculeName: Lrc4 / type: protein_or_peptide / ID: 26 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Porphyridium purpureum (eukaryote)
Molecular weightTheoretical: 16.91526 KDa
SequenceString:
MAFVACGPLR AGEGGARLGA RKAACSMQLA PPGIPPGEDA RNNQSLRQYV ARPVETYQKR SFATPLPLTW TGETETVGAF DVVVPPQEK DLPVSGEATS AFVKYSDMVR AERKAALQAL LSASAAGEGR PTCGAEGRKF VSNANPVLVN GVKCVEYWRK

UniProtKB: Uncharacterized protein

+
Macromolecule #27: LRC5

MacromoleculeName: LRC5 / type: protein_or_peptide / ID: 27 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Porphyridium purpureum (eukaryote)
Molecular weightTheoretical: 30.272959 KDa
SequenceString: MAFVSGAGVA VPAGAKASAP LCALRMSGYG DYSYSTDRTK GHVNQYYVDK ARSRSDWGNR NVLPASEGDA VLGRTAKGAV AVPEFGIPQ LDDPVLGFGP DSMVDPRIAE ADGAVWRWDA GFVDESMTLA SCADISDEAV ADEAFAKFRG SVLAERGAMI T KAESATAS ...String:
MAFVSGAGVA VPAGAKASAP LCALRMSGYG DYSYSTDRTK GHVNQYYVDK ARSRSDWGNR NVLPASEGDA VLGRTAKGAV AVPEFGIPQ LDDPVLGFGP DSMVDPRIAE ADGAVWRWDA GFVDESMTLA SCADISDEAV ADEAFAKFRG SVLAERGAMI T KAESATAS VITSLRDGLY SGEAQLLTAS GQRLANVAGQ EKIATISGYT WDGQPQTEIP GKPFVKSIGA MDYMDGVEGG DV VAAKVGA FWKPKAPKEV PYKRPMGANT PELPYNTVPR LVQAAGLAVQ E

UniProtKB: Uncharacterized protein

+
Macromolecule #28: FAS1 domain-containing protein

MacromoleculeName: FAS1 domain-containing protein / type: protein_or_peptide / ID: 28 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Porphyridium purpureum (eukaryote)
Molecular weightTheoretical: 26.620727 KDa
SequenceString: MAGFVASTGA VLRKQQVDAA PVCERASSRA RAVAPLSMAR TAYPYTGSGY GSAGVPYGQD TYGYKATTAK SITETAAQAG VFNTFVKLL NESGVEKLVE QAGPYTVFAP TDDAFAALLE PHSFNKLATL LRPENNDALR KVLMHHVIPG AFTSASLMDR A VTVKSLAG ...String:
MAGFVASTGA VLRKQQVDAA PVCERASSRA RAVAPLSMAR TAYPYTGSGY GSAGVPYGQD TYGYKATTAK SITETAAQAG VFNTFVKLL NESGVEKLVE QAGPYTVFAP TDDAFAALLE PHSFNKLATL LRPENNDALR KVLMHHVIPG AFTSASLMDR A VTVKSLAG EPISIMGLNK LVTAGTAKVV RADVPCANGC IIHAVSSVII PPNYVPVPQP TKPVFPRSVI AEIAKLPTPR QA LGLDPAP SKIVKY

UniProtKB: FAS1 domain-containing protein

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Macromolecule #29: PHYCOERYTHROBILIN

MacromoleculeName: PHYCOERYTHROBILIN / type: ligand / ID: 29 / Number of copies: 1298 / Formula: PEB
Molecular weightTheoretical: 588.694 Da
Chemical component information

ChemComp-PEB:
PHYCOERYTHROBILIN

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Macromolecule #30: PHYCOUROBILIN

MacromoleculeName: PHYCOUROBILIN / type: ligand / ID: 30 / Number of copies: 40 / Formula: PUB
Molecular weightTheoretical: 590.71 Da
Chemical component information

ChemComp-CYB:
PHYCOUROBILIN

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Macromolecule #31: PHYCOCYANOBILIN

MacromoleculeName: PHYCOCYANOBILIN / type: ligand / ID: 31 / Number of copies: 120 / Formula: CYC
Molecular weightTheoretical: 588.694 Da
Chemical component information

ChemComp-CYC:
PHYCOCYANOBILIN

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Macromolecule #32: phenylmethanesulfonic acid

MacromoleculeName: phenylmethanesulfonic acid / type: ligand / ID: 32 / Number of copies: 4 / Formula: PMS
Molecular weightTheoretical: 172.202 Da
Chemical component information

ChemComp-IOX:
phenylmethanesulfonic acid

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

-
Sample preparation

BufferpH: 7
Component:
ConcentrationFormulaName
0.75 mol/LNa2HPO4disodium hydrogen phosphate
0.75 mol/LKH2PO4monopotassium phosphate
GridModel: PELCO Ultrathin Carbon with Lacey Carbon / Material: COPPER / Mesh: 200 / Support film - Material: CARBON / Support film - topology: LACEY / Support film - Film thickness: 2 / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 30 sec. / Pretreatment - Atmosphere: AIR / Pretreatment - Pressure: 0.1 kPa
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 18 K / Instrument: FEI VITROBOT MARK IV

-
Electron microscopy

MicroscopeFEI TITAN KRIOS
TemperatureMin: 70.0 K / Max: 70.0 K
Specialist opticsEnergy filter - Name: GIF Quantum ER
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: SUPER-RESOLUTION / Digitization - Dimensions - Width: 4096 pixel / Digitization - Dimensions - Height: 4096 pixel / Digitization - Frames/image: 1-32 / Number grids imaged: 2 / Number real images: 6438 / Average exposure time: 8.2 sec. / Average electron dose: 50.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 100.0 µm / Calibrated defocus max: 2.3000000000000003 µm / Calibrated defocus min: 1.3 µm / Calibrated magnification: 130000 / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 2.3000000000000003 µm / Nominal defocus min: 1.3 µm / Nominal magnification: 130000
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

+
Image processing

Particle selectionNumber selected: 315046
CTF correctionSoftware - Name: CTFFIND (ver. 4.15) / Type: NONE
Startup modelType of model: PDB ENTRY
PDB model - PDB ID:

6kgx

Final reconstructionNumber classes used: 2 / Applied symmetry - Point group: C2 (2 fold cyclic) / Algorithm: FOURIER SPACE / Resolution.type: BY AUTHOR / Resolution: 3.0 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 3.1beta) / Number images used: 87399
Initial angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3.1beta)
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3.1beta)
Final 3D classificationNumber classes: 5 / Avg.num./class: 87399 / Software - Name: RELION (ver. 3.1beta)

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Atomic model buiding 1

Initial modelPDB ID:

6kgx


Chain - Source name: PDB / Chain - Initial model type: experimental model
RefinementSpace: REAL / Protocol: RIGID BODY FIT / Overall B value: 62.05 / Target criteria: 0,99
Output model

PDB-7ezx:
Structure of the phycobilisome from the red alga Porphyridium purpureum in Middle Light

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