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Yorodumi- EMDB-31246: Cryo-electron tomogram of phycobilisome-photosystem II supercompl... -
+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-31246 | |||||||||||||||
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Title | Cryo-electron tomogram of phycobilisome-photosystem II supercomplex on the thylakoid | |||||||||||||||
Map data | Cryo-electron tomogram of phycobilisome-photosystem II supercomplex on the thylakoid | |||||||||||||||
Sample |
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Biological species | Porphyridium purpureum (eukaryote) | |||||||||||||||
Method | electron tomography / cryo EM | |||||||||||||||
Authors | Sui SF / Li XM / Li MJ / Ma JF | |||||||||||||||
Funding support | China, 4 items
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Citation | Journal: To Be Published Title: In situ cryo-ET structure of phycobilisome-photosystem II supercomplex from red alga Authors: Sui SF / Li XM / Li MJ / Ma JF | |||||||||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_31246.map.gz | 463.1 MB | EMDB map data format | |
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Header (meta data) | emd-31246-v30.xml emd-31246.xml | 8.5 KB 8.5 KB | Display Display | EMDB header |
Images | emd_31246.png | 354.1 KB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-31246 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-31246 | HTTPS FTP |
-Validation report
Summary document | emd_31246_validation.pdf.gz | 402.5 KB | Display | EMDB validaton report |
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Full document | emd_31246_full_validation.pdf.gz | 402 KB | Display | |
Data in XML | emd_31246_validation.xml.gz | 4.5 KB | Display | |
Data in CIF | emd_31246_validation.cif.gz | 5 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-31246 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-31246 | HTTPS FTP |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_31246.map.gz / Format: CCP4 / Size: 535.8 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||
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Annotation | Cryo-electron tomogram of phycobilisome-photosystem II supercomplex on the thylakoid | ||||||||||||||||||||||||||||||||
Projections & slices | Image control
Images are generated by Spider. generated in cubic-lattice coordinate | ||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 13.684 Å | ||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Sample components
-Entire : phycobilisome-photosystem II supercomplex
Entire | Name: phycobilisome-photosystem II supercomplex |
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Components |
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-Supramolecule #1: phycobilisome-photosystem II supercomplex
Supramolecule | Name: phycobilisome-photosystem II supercomplex / type: cell / ID: 1 / Parent: 0 / Details: phycobilisome, photosystem II |
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Source (natural) | Organism: Porphyridium purpureum (eukaryote) |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | electron tomography |
Aggregation state | cell |
-Sample preparation
Buffer | pH: 7 |
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Vitrification | Cryogen name: ETHANE |
Sectioning | Focused ion beam - Instrument: OTHER / Focused ion beam - Ion: OTHER / Focused ion beam - Voltage: 5 kV / Focused ion beam - Current: 0.1 nA / Focused ion beam - Duration: 3600 sec. / Focused ion beam - Temperature: 191 K / Focused ion beam - Initial thickness: 1000 nm / Focused ion beam - Final thickness: 200 nm Focused ion beam - Details: The value given for _emd_sectioning_focused_ion_beam.instrument is FEI Quanta FIB. This is not in a list of allowed values {'OTHER', 'DB235'} so OTHER is written into the XML file. |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 120.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
-Image processing
Final reconstruction | Number images used: 42 |
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