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- EMDB-30923: CryoEM Structure of Mannose Transporter ManYZ and Microcin E492 (... -
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Open data
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Basic information
Entry | Database: EMDB / ID: EMD-30923 | |||||||||
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Title | CryoEM Structure of Mannose Transporter ManYZ and Microcin E492 (MceA) complex | |||||||||
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![]() | MceA / Microcin E492 / Bacteriocin / PTS / ManYZ / transporter / Mannose / PROTEIN TRANSPORT | |||||||||
Function / homology | ![]() mannose transmembrane transport / protein-N(PI)-phosphohistidine-mannose phosphotransferase system transporter activity / glucose import across plasma membrane / cytolysis / phosphoenolpyruvate-dependent sugar phosphotransferase system / transmembrane transporter complex / monoatomic ion transport / defense response to bacterium / membrane / plasma membrane Similarity search - Function | |||||||||
Biological species | ![]() ![]() ![]() ![]() ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 2.28 Å | |||||||||
![]() | Wang JW / Zeng JW | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Structure of the mannose phosphotransferase system (man-PTS) complexed with microcin E492, a pore-forming bacteriocin. Authors: Kai Huang / Jianwei Zeng / Xueli Liu / Tianyu Jiang / Jiawei Wang / ![]() | |||||||||
History |
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Structure visualization
Movie |
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Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 14 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 12.5 KB 12.5 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 14 KB | Display | ![]() |
Images | ![]() | 67.2 KB | ||
Filedesc metadata | ![]() | 5.7 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 359.5 KB | Display | ![]() |
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Full document | ![]() | 359 KB | Display | |
Data in XML | ![]() | 13.7 KB | Display | |
Data in CIF | ![]() | 18.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7dyrMC M: atomic model generated by this map C: citing same article ( |
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Similar structure data |
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Voxel size | X=Y=Z: 1.061 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
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Sample components
-Entire : ManYZ trimer complex with Microcin E492 (MceA)
Entire | Name: ManYZ trimer complex with Microcin E492 (MceA) |
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Components |
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-Supramolecule #1: ManYZ trimer complex with Microcin E492 (MceA)
Supramolecule | Name: ManYZ trimer complex with Microcin E492 (MceA) / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#3 |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Theoretical: 0.53 kDa/nm |
-Macromolecule #1: PTS system mannose-specific EIIC component
Macromolecule | Name: PTS system mannose-specific EIIC component / type: protein_or_peptide / ID: 1 / Number of copies: 3 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Theoretical: 27.647621 KDa |
Recombinant expression | Organism: ![]() ![]() |
Sequence | String: MEITTLQIVL VFIVACIAGM GSILDEFQFH RPLIACTLVG IVLGDMKTGI IIGGTLEMIA LGWMNIGAAV APDAALASII STILVIAGH QSIGAGIALA IPLAAAGQVL TIIVRTITVA FQHAADKAAD NGNLTAISWI HVSSLFLQAM RVAIPAVIVA L SVGTSEVQ ...String: MEITTLQIVL VFIVACIAGM GSILDEFQFH RPLIACTLVG IVLGDMKTGI IIGGTLEMIA LGWMNIGAAV APDAALASII STILVIAGH QSIGAGIALA IPLAAAGQVL TIIVRTITVA FQHAADKAAD NGNLTAISWI HVSSLFLQAM RVAIPAVIVA L SVGTSEVQ NMLNAIPEVV TNGLNIAGGM IVVVGYAMVI NMMRAGYLMP FFYLGFVTAA FTNFNLVALG VIGTVMAVLY IQ LSPKYNR VAGAPAQAAG NNDLDNELD UniProtKB: PTS system mannose-specific EIIC component |
-Macromolecule #2: PTS system mannose-specific EIID component
Macromolecule | Name: PTS system mannose-specific EIID component / type: protein_or_peptide / ID: 2 / Number of copies: 3 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Theoretical: 30.983373 KDa |
Recombinant expression | Organism: ![]() ![]() |
Sequence | String: MVDTTQTTTE KKLTQSDIRG VFLRSNLFQG SWNFERMQAL GFCFSMVPAI RRLYPENNEA RKQAIRRHLE FFNTQPFVAA PILGVTLAL EEQRANGAEI DDGAINGIKV GLMGPLAGVG DPIFWGTVRP VFAALGAGIA MSGSLLGPLL FFILFNLVRL A TRYYGVAY ...String: MVDTTQTTTE KKLTQSDIRG VFLRSNLFQG SWNFERMQAL GFCFSMVPAI RRLYPENNEA RKQAIRRHLE FFNTQPFVAA PILGVTLAL EEQRANGAEI DDGAINGIKV GLMGPLAGVG DPIFWGTVRP VFAALGAGIA MSGSLLGPLL FFILFNLVRL A TRYYGVAY GYSKGIDIVK DMGGGFLQKL TEGASILGLF VMGALVNKWT HVNIPLVVSR ITDQTGKEHV TTVQTILDQL MP GLVPLLL TFACMWLLRK KVNPLWIIVG FFVIGIAGYA CGLLGL UniProtKB: PTS system mannose-specific EIID component |
-Macromolecule #3: Microcin E492
Macromolecule | Name: Microcin E492 / type: protein_or_peptide / ID: 3 / Number of copies: 3 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 7.887492 KDa |
Recombinant expression | Organism: ![]() ![]() |
Sequence | String: GETDPNTQLL NDLGNNMAWG AALGAPGGLG SAALGAAGGA LQTVGQGLID HGPVNVPIPV LIGPSWNGSG SGYNSATSSS GSGS UniProtKB: Microcin E492 |
-Macromolecule #4: alpha-D-mannopyranose
Macromolecule | Name: alpha-D-mannopyranose / type: ligand / ID: 4 / Number of copies: 3 / Formula: MAN |
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Molecular weight | Theoretical: 180.156 Da |
Chemical component information | ![]() ChemComp-MAN: |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 8 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: SUPER-RESOLUTION / Average electron dose: 50.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |