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Open data
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Basic information
Entry | Database: EMDB / ID: EMD-30868 | |||||||||
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Title | liver ribosome | |||||||||
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Biological species | Ribosome display vector pRDV (others) | |||||||||
Method | subtomogram averaging / cryo EM / Resolution: 18.0 Å | |||||||||
![]() | Zhang JG / Zhang DY / Sun L / Ji G / Huang XJ / Niu TX / Xu JS / Ma CY / Zhu Y / Gao N ...Zhang JG / Zhang DY / Sun L / Ji G / Huang XJ / Niu TX / Xu JS / Ma CY / Zhu Y / Gao N / Xu W / Sun F | |||||||||
![]() | ![]() Title: VHUT-cryo-FIB, a method to fabricate frozen hydrated lamellae from tissue specimens for in situ cryo-electron tomography Authors: Zhang J / Zhang D / Sun L / Ji G / Huang X / Niu T / Xu J / Ma C / Zhu Y / Gao N / Xu W / Sun F | |||||||||
History |
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Structure visualization
Movie |
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Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 4.8 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 7.5 KB 7.5 KB | Display Display | ![]() |
Images | ![]() | 237.7 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 326.8 KB | Display | ![]() |
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Full document | ![]() | 326.4 KB | Display | |
Data in XML | ![]() | 6.3 KB | Display | |
Data in CIF | ![]() | 7.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data | |
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EM raw data | ![]() Data size: 112.1 Data #1: ungaligned multi-frame micrographs of ribosome [micrographs - multiframe]) |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
File | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Voxel size | X=Y=Z: 2.7 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
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Sample components
-Entire : liver ribosome
Entire | Name: liver ribosome |
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Components |
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-Supramolecule #1: liver ribosome
Supramolecule | Name: liver ribosome / type: complex / ID: 1 / Parent: 0 |
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Source (natural) | Organism: Ribosome display vector pRDV (others) |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | subtomogram averaging |
Aggregation state | tissue |
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Sample preparation
Buffer | pH: 7 |
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Vitrification | Cryogen name: OTHER |
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Electron microscopy
Microscope | FEI TITAN |
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Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 3.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD |
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Image processing
Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 18.0 Å / Resolution method: FSC 0.143 CUT-OFF / Number subtomograms used: 13 |
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Extraction | Number tomograms: 13 / Number images used: 13 |
Final angle assignment | Type: MAXIMUM LIKELIHOOD |