EMDB-30637: Cryo-EM structure of the activated spliceosome (Bact complex) at ...

Basic information

• Entry: Database: EMDB / ID: EMD-30637
• Title: Cryo-EM structure of the activated spliceosome (Bact complex) at an atomic resolution of 2.5 angstrom
• Map data: overall EM map of the yeast activated spliceosome (Bact complex) at the average resolution of 2.5 angstrom

Sample

• Complex: the activated spliceosome Bact complex
  • Protein or peptide: x 43 types
  • RNA: x 4 types
  • Ligand: x 3 types
• Ligand: x 3 types

• Keywords: RNA splicing / spliceosome / Bact complex / Prp2 / Spp2 / ATPase/helicase / activation / SPLICING

Function / homology

Function:

maintenance of RNA location / RES complex / snoRNA splicing / generation of catalytic spliceosome for first transesterification step / U4/U6 snRNP / 7-methylguanosine cap hypermethylation / U2-type catalytic step 1 spliceosome / Prp19 complex / spliceosomal tri-snRNP complex / small nuclear ribonucleoprotein complex / ATP-dependent activity, acting on RNA / SMN-Sm protein complex / mRNA cis splicing, via spliceosome / U2-type spliceosomal complex / U2-type prespliceosome assembly / commitment complex / U4 snRNP / U2 snRNP / U1 snRNP / U2-type prespliceosome / precatalytic spliceosome / spliceosomal complex assembly / generation of catalytic spliceosome for second transesterification step / ATPase activator activity / mRNA 5'-splice site recognition / U5 snRNP / U2 snRNA binding / spliceosomal snRNP assembly / translation elongation factor activity / mRNA export from nucleus / U4/U6 x U5 tri-snRNP complex / catalytic step 2 spliceosome / RNA splicing / helicase activity / spliceosomal complex / mRNA splicing, via spliceosome / mRNA processing / RNA helicase activity / nucleic acid binding / RNA helicase / mRNA binding / GTPase activity / GTP binding / ATP hydrolysis activity / DNA binding / RNA binding / ATP binding / metal ion binding / nucleus / cytoplasm

Domain/homology:

U2 small nuclear ribonucleoprotein A' / Pre-mRNA-splicing factor Spp2-like / Spp2/MOS2, G-patch domain / Pre-mRNA-splicing factor CWC24-like / G-patch domain / cwf21 / Torus domain / Pre-mRNA-splicing factor Cwc2, RNA recognition motif / Torus domain / Splicing factor 3A subunit 1 / Splicing factor 3A subunit 1, conserved domain / Pre-mRNA splicing factor PRP21 like protein / SWAP/Surp / SWAP/Surp superfamily / Surp module / SURP motif repeat profile. / Suppressor-of-White-APricot splicing regulator / mRNA splicing factor Cwf21 domain / cwf21 domain / Domain of unknown function DUF382 / Domain of unknown function (DUF382) / RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain) / BUD31/G10-related, conserved site / : / : / G10 protein signature 1. / G10 protein signature 2. / G-patch domain / glycine rich nucleic binding domain / PHF5-like / PHF5-like protein / PSP, proline-rich / PSP / proline-rich domain in spliceosome associated proteins / Pre-mRNA-splicing factor Cwf15/Cwc15 / HAT (Half-A-TPR) repeat / Cwf15/Cwc15 cell cycle control protein / Splicing factor 3B subunit 5/RDS3 complex subunit 10 / Splicing factor 3B subunit 10 (SF3b10) / Zinc finger C-x8-C-x5-C-x3-H type (and similar) / Pre-mRNA-splicing factor Cwc2/Slt11 / G10 protein / Pre-mRNA-splicing factor BUD31 / Pre-mRNA splicing factor component Cdc5p/Cef1, C-terminal / pre-mRNA splicing factor component / : / U2A'/phosphoprotein 32 family A, C-terminal / occurring C-terminal to leucine-rich repeats / Leucine-rich repeat / Pre-mRNA-splicing factor Syf1-like / Snu114, GTP-binding domain / Zinc finger, CCCH-type superfamily / 116kDa U5 small nuclear ribonucleoprotein component, N-terminal / 116kDa U5 small nuclear ribonucleoprotein component, C-terminal / 116 kDa U5 small nuclear ribonucleoprotein component N-terminus / : / Helicase associated domain (HA2), ratchet-like / zinc finger / DEAD-box helicase, OB fold / Oligonucleotide/oligosaccharide-binding (OB)-fold / Helicase-associated domain / Helicase associated domain (HA2), winged-helix / Helicase associated domain (HA2) Add an annotation / Small nuclear ribonucleoprotein Sm D3 / Small nuclear ribonucleoprotein Sm D2 / Small nuclear ribonucleoprotein E / Small nuclear ribonucleoprotein G / Small nuclear ribonucleoprotein F / Sm-like protein Lsm7/SmG / Like-Sm (LSM) domain containing protein, LSm4/SmD1/SmD3 / Sm-like protein Lsm6/SmF / Myb-type HTH DNA-binding domain profile. / Zinc finger, CCCH-type / Zinc finger C3H1-type profile. / LSM domain / LSM domain, eukaryotic/archaea-type / snRNP Sm proteins / HAT (Half-A-TPR) repeat / HAT (Half-A-TPR) repeats / Forkhead associated domain / Forkhead-associated (FHA) domain profile. / FHA domain / Myb domain / : / Forkhead-associated (FHA) domain / Sm domain profile. / Myb-like DNA-binding domain / DNA/RNA helicase, ATP-dependent, DEAH-box type, conserved site / DEAH-box subfamily ATP-dependent helicases signature. / Translation elongation factor EFG/EF2, domain IV / Elongation factor G, domain IV / Elongation factor G, domain IV / LSM domain superfamily / Elongation factor G C-terminus / SMAD/FHA domain superfamily / Elongation factor EFG, domain V-like / Elongation factor G C-terminus / EF-G domain III/V-like / SANT SWI3, ADA2, N-CoR and TFIIIB'' DNA-binding domains / SANT/Myb domain

Component:

: / : / Pre-mRNA-splicing factor CLF1 / : / : / Pre-mRNA-splicing factor CWC24 / SNU114 isoform 1 / : / : / : / Pre-mRNA-splicing factor CWC21 / : / BJ4_G0014900.mRNA.1.CDS.1 / : / : / : / Pre-mRNA-splicing factor CWC2 / : / RDS3 isoform 1 / : / U2 small nuclear ribonucleoprotein A' / : / MSL1 isoform 1 / Small nuclear ribonucleoprotein Sm D3 / BUD31 isoform 1 / : / Sm protein F / : / Small nuclear ribonucleoprotein E / Small nuclear ribonucleoprotein Sm D2 / HLJ1_G0022770.mRNA.1.CDS.1 / HLJ1_G0043010.mRNA.1.CDS.1 / : / : / RDS3 complex subunit 10 / Pre-mRNA-splicing factor ATP-dependent RNA helicase-like protein PRP2 / Pre-mRNA-splicing factor PRP21 / Small nuclear ribonucleoprotein G / Pre-mRNA-splicing factor SPP2 / Pre-mRNA-splicing factor CEF1 / Pre-mRNA-splicing factor CWC15 / Pre-mRNA leakage protein 1

• Biological species: Saccharomyces cerevisiae (brewer's yeast) / Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
• Method: single particle reconstruction / cryo EM / Resolution: 2.5 Å
• Authors: Bai R / Wan R

Funding support

China, 1 items

Organization	Grant number	Country
National Natural Science Foundation of China (NSFC)	31930059	China

• Citation: Journal: Science / Year: 2021; Title: Mechanism of spliceosome remodeling by the ATPase/helicase Prp2 and its coactivator Spp2.; Authors: Rui Bai / Ruixue Wan / Chuangye Yan / Qi Jia / Jianlin Lei / Yigong Shi / ; Abstract: Spliceosome remodeling, executed by conserved adenosine triphosphatase (ATPase)/helicases including Prp2, enables precursor messenger RNA (pre-mRNA) splicing. However, the structural basis for the function of the ATPase/helicases remains poorly understood. Here, we report atomic structures of Prp2 in isolation, Prp2 complexed with its coactivator Spp2, and Prp2-loaded activated spliceosome and the results of structure-guided biochemical analysis. Prp2 weakly associates with the spliceosome and cannot function without Spp2, which stably associates with Prp2 and anchors on the spliceosome, thus tethering Prp2 to the activated spliceosome and allowing Prp2 to function. Pre-mRNA is loaded into a featured channel between the N and C halves of Prp2, where Leu from the N half and Arg from the C half prevent backward sliding of pre-mRNA toward its 5'-end. Adenosine 5'-triphosphate binding and hydrolysis trigger interdomain movement in Prp2, which drives unidirectional stepwise translocation of pre-mRNA toward its 3'-end. These conserved mechanisms explain the coupling of spliceosome remodeling to pre-mRNA splicing.

History

Deposition	Oct 26, 2020	-
Header (metadata) release	Mar 17, 2021	-
Map release	Mar 17, 2021	-
Update	Mar 27, 2024	-
Current status	Mar 27, 2024	Processing site: PDBj / Status: Released

Map

• File: Download / File: emd_30637.map.gz / Format: CCP4 / Size: 244.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Annotation: overall EM map of the yeast activated spliceosome (Bact complex) at the average resolution of 2.5 angstrom
• Voxel size: X=Y=Z: 1.06 Å

Density

Contour Level	By AUTHOR: 0.015 / Movie #1: 0.015
Minimum - Maximum	-0.119457714 - 0.25123748
Average (Standard dev.)	-0.00001784702 (±0.005586911)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 400 400 400 Spacing 400 400 400
Cell	A=B=C: 423.99997 Å α=β=γ: 90.0 °

CCP4 map header:

mode	Image stored as Reals
Å/pix. X/Y/Z	1.06	1.06	1.06
M x/y/z	400	400	400
origin x/y/z	0.000	0.000	0.000
length x/y/z	424.000	424.000	424.000
α/β/γ	90.000	90.000	90.000
MAP C/R/S	1	2	3
start NC/NR/NS	0	0	0
NC/NR/NS	400	400	400
D min/max/mean	-0.119	0.251	-0.000

Supplemental data

Additional map: EM map of Prp2 region in the Bact complex

• File: emd_30637_additional_1.map
• Annotation: EM map of Prp2 region in the Bact complex

Sample components

Entire : the activated spliceosome Bact complex

• Entire: Name: the activated spliceosome Bact complex

Components

• Complex: the activated spliceosome Bact complex
  • Protein or peptide: PRP8 isoform 1
  • RNA: U5 snRNAU5 spliceosomal RNA
  • Protein or peptide: SNU114 isoform 1
  • Protein or peptide: BRR2 isoform 1
  • Protein or peptide: Small nuclear ribonucleoprotein Sm D3
  • Protein or peptide: BJ4_G0014900.mRNA.1.CDS.1
  • Protein or peptide: SMD1 isoform 1
  • Protein or peptide: BJ4_G0037700.mRNA.1.CDS.1
  • Protein or peptide: Small nuclear ribonucleoprotein E
  • Protein or peptide: Sm protein F
  • Protein or peptide: Small nuclear ribonucleoprotein G
  • RNA: U6 snRNAU6 spliceosomal RNA
  • RNA: pre-mRNAPrimary transcript
  • RNA: U2 snRNAU2 spliceosomal RNA
  • Protein or peptide: HLJ1_G0053790.mRNA.1.CDS.1
  • Protein or peptide: BJ4_G0027490.mRNA.1.CDS.1
  • Protein or peptide: Small nuclear ribonucleoprotein Sm D3
  • Protein or peptide: PRP9 isoform 1
  • Protein or peptide: Pre-mRNA-splicing factor PRP21
  • Protein or peptide: PRP11 isoform 1
  • Protein or peptide: HSH155 isoform 1
  • Protein or peptide: HLJ1_G0043010.mRNA.1.CDS.1
  • Protein or peptide: RSE1 isoform 1
  • Protein or peptide: HSH49 isoform 1
  • Protein or peptide: BJ4_G0056610.mRNA.1.CDS.1
  • Protein or peptide: RDS3 complex subunit 10
  • Protein or peptide: Pre-mRNA-splicing factor CEF1
  • Protein or peptide: Pre-mRNA-processing factor 19
  • Protein or peptide: SNT309 isoform 1
  • Protein or peptide: BUD31 isoform 1
  • Protein or peptide: HLJ1_G0054350.mRNA.1.CDS.1
  • Protein or peptide: Pre-mRNA-processing protein 45
  • Protein or peptide: Pre-mRNA-splicing factor SLT11
  • Protein or peptide: Pre-mRNA-splicing factor CWC2
  • Protein or peptide: Pre-mRNA-splicing factor CWC15
  • Protein or peptide: Pre-mRNA leakage protein 1
  • Protein or peptide: SX2_G0027210.mRNA.1.CDS.1
  • Protein or peptide: Pre-mRNA-splicing factor CWC26
  • Protein or peptide: CDC40 isoform 1
  • Protein or peptide: Pre-mRNA-splicing factor CWC21
  • Protein or peptide: CWC22 isoform 1
  • Protein or peptide: Pre-mRNA-splicing factor CWC24
  • Protein or peptide: CWC27 isoform 1
  • Protein or peptide: Pre-mRNA-splicing factor SPP2
  • Protein or peptide: CLF1 isoform 1
  • Protein or peptide: SYF1 isoform 1
  • Protein or peptide: Pre-mRNA-splicing factor ATP-dependent RNA helicase-like protein PRP2
  • Ligand: INOSITOL HEXAKISPHOSPHATEPhytic acid
  • Ligand: GUANOSINE-5'-TRIPHOSPHATEGuanosine triphosphate
  • Ligand: MAGNESIUM ION
• Ligand: CALCIUM IONCalcium
• Ligand: ZINC ION
• Ligand: water

Supramolecule #1: the activated spliceosome Bact complex

• Supramolecule: Name: the activated spliceosome Bact complex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#50
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)

Macromolecule #1: PRP8 isoform 1

• Macromolecule: Name: PRP8 isoform 1 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 279.867469 KDa

Sequence

String:

MSGLPPPPPG FEEDSDLALP PPPPPPPGYE IEELDNPMVP SSVNEDTFLP PPPPPPSNFE INAEEIVDFT LPPPPPPPGL DELETKAEK KVELHGKRKL DIGKDTFVTR KSRKRAKKMT KKAKRSNLYT PKAEMPPEHL RKIINTHSDM ASKMYNTDKK A FLGALKYL PHAILKLLEN MPHPWEQAKE VKVLYHTSGA ITFVNETPRV IEPVYTAQWS ATWIAMRREK RDRTHFKRMR FP PFDDDEP PLSYEQHIEN IEPLDPINLP LDSQDDEYVK DWLYDSRPLE EDSKKVNGTS YKKWSFDLPE MSNLYRLSTP LRD EVTDKN YYYLFDKKSF FNGKALNNAI PGGPKFEPLY PREEEEDYNE FNSIDRVIFR VPIRSEYKVA FPHLYNSRPR SVRI PWYNN PVSCIIQNDE EYDTPALFFD PSLNPIPHFI DNNSSLNVSN TKENGDFTLP EDFAPLLAEE EELILPNTKD AMSLY HSPF PFNRTKGKMV RAQDVALAKK WFLQHPDEEY PVKVKVSYQK LLKNYVLNEL HPTLPTNHNK TKLLKSLKNT KYFQQT TID WVEAGLQLCR QGHNMLNLLI HRKGLTYLHL DYNFNLKPTK TLTTKERKKS RLGNSFHLMR ELLKMMKLIV DTHVQFR LG NVDAFQLADG IHYILNHIGQ LTGIYRYKYK VMHQIRACKD LKHIIYYKFN KNLGKGPGCG FWQPAWRVWL NFLRGTIP L LERYIGNLIT RQFEGRSNEI VKTTTKQRLD AYYDLELRNS VMDDILEMMP ESIRQKKART ILQHLSEAWR CWKANIPWD VPGMPAPIKK IIERYIKSKA DAWVSAAHYN RERIKRGAHV EKTMVKKNLG RLTRLWIKNE QERQRQIQKN GPEITPEEAT TIFSVMVEW LESRSFSPIP FPPLTYKNDT KILVLALEDL KDVYASKVRL NASEREELAL IEEAYDNPHD TLNRIKKYLL T QRVFKPVD ITMMENYQNI SPVYSVDPLE KITDAYLDQY LWYEADQRKL FPNWIKPSDS EIPPLLVYKW TQGINNLSEI WD VSRGQSA VLLETTLGEM AEKIDFTLLN RLLRLIVDPN IADYITAKNN VVINFKDMSH VNKYGLIRGL KFASFIFQYY GLV IDLLLL GQERATDLAG PANNPNEFMQ FKSKEVEKAH PIRLYTRYLD RIYMLFHFEE DEGEELTDEY LAENPDPNFE NSIG YNNRK CWPKDSRMRL IRQDVNLGRA VFWEIQSRVP TSLTSIKWEN AFVSVYSKNN PNLLFSMCGF EVRILPRQRM EEVVS NDEG VWDLVDERTK QRTAKAYLKV SEEEIKKFDS RIRGILMASG STTFTKVAAK WNTSLISLFT YFREAIVATE PLLDIL VKG ETRIQNRVKL GLNSKMPTRF PPAVFYTPKE LGGLGMISAS HILIPASDLS WSKQTDTGIT HFRAGMTHED EKLIPTI FR YITTWENEFL DSQRVWAEYA TKRQEAIQQN RRLAFEELEG SWDRGIPRIS TLFQRDRHTL AYDRGHRIRR EFKQYSLE R NSPFWWTNSH HDGKLWNLNA YRTDVIQALG GIETILEHTL FKGTGFNSWE GLFWEKASGF EDSMQFKKLT HAQRTGLSQ IPNRRFTLWW SPTINRANVY VGFLVQLDLT GIFLHGKIPT LKISLIQIFR AHLWQKIHES IVFDICQILD GELDVLQIES VTKETVHPR KSYKMNSSAA DITMESVHEW EVSKPSLLHE TNDSFKGLIT NKMWFDVQLR YGDYDSHDIS RYVRAKFLDY T TDNVSMYP SPTGVMIGID LAYNMYDAYG NWFNGLKPLI QNSMRTIMKA NPALYVLRER IRKGLQIYQS SVQEPFLNSS NY AELFNND IKLFVDDTNV YRVTVHKTFE GNVATKAING CIFTLNPKTG HLFLKIIHTS VWAGQKRLSQ LAKWKTAEEV SAL VRSLPK EEQPKQIIVT RKAMLDPLEV HMLDFPNIAI RPTELRLPFS AAMSIDKLSD VVMKATEPQM VLFNIYDDWL DRIS SYTAF SRLTLLLRAL KTNEESAKMI LLSDPTITIK SYHLWPSFTD EQWITIESQM RDLILTEYGR KYNVNISALT QTEIK DIIL GQNIKAPSVK RQKMAELEAA RSEKQNDEEA AGASTVMKTK TINAQGEEIV VVASADYESQ TFSSKNEWRK SAIANT LLY LRLKNIYVSA DDFVEEQNVY VLPKNLLKKF IEISDVKIQV AAFIYGMSAK DHPKVKEIKT VVLVPQLGHV GSVQISN IP DIGDLPDTEG LELLGWIHTQ TEELKFMAAS EVATHSKLFA DKKRDCIDIS IFSTPGSVSL SAYNLTDEGY QWGEENKD I MNVLSEGFEP TFSTHAQLLL SDRITGNFII PSGNVWNYTF MGTAFNQEGD YNFKYGIPLE FYNEMHRPVH FLQFSELAG DEELEAEQID VFS

UniProtKB: UNIPROTKB: A0A6A5PW68

Macromolecule #3: SNU114 isoform 1

• Macromolecule: Name: SNU114 isoform 1 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 114.174008 KDa

Sequence

String:

MEGDDLFDEF GNLIGVDPFD SDEEESVLDE QEQYQTNTFE GSGNNNEIES RQLTSLGSKK ELGISLEHPY GKEVEVLMET KNTQSPQTP LVEPVTERTK LQEHTIFTQL KKNIPKTRYN RDYMLSMANI PERIINVGVI GPLHSGKTSL MDLLVIDSHK R IPDMSKNV ELGWKPLRYL DNLKQEIDRG LSIKLNGSTL LCTDLESKSR MINFLDAPGH VNFMDETAVA LAASDLVLIV ID VVEGVTF VVEQLIKQSI KNNVAMCFVI NKLDRLILDL KLPPMDAYLK LNHIIANINS FTKGNVFSPI DNNIIFASTK LGF TFTIKE FVSYYYAHSI PSSKIDDFTT RLWGSVYYHK GNFRTKPFEN VEKYPTFVEF ILIPLYKIFS YALSMEKDKL KNLL RSNFR VNLSQEALQY DPQPFLKHVL QLIFRQQTGL VDAITRCYQP FELFDNKTAH LSIPGKSTPE GTLWAHVLKT VDYGG AEWS LVRIYSGLLK RGDTVRILDT SQSESRQKRQ LHDISKTETS NEDEDEDDET PSCEVEEIGL LGGRYVYPVH EAHKGQ IVL IKGISSAYIK SATLYSVKSK EDMKQLKFFK PLDYITEAVF KIVLQPLLPR ELPKLLDALN KISKYYPGVI IKVEESG EH VILGNGELYM DCLLYDLRAS YAKIEIKISD PLTVFSESCS NESFASIPVS NSISRLGEEN LPGLSISVAA EPMDSKMI Q DLSRNTLGKG QNCLDIDGIM DNPRKLSKIL RTEYGWDSLA SRNVWSFYNG NVLINDTLPD EISPELLSKY KEQIIQGFY WAVKEGPLAE EPIYGVQYKL LSISVPSDVN IDVMKSQIIP LMKKACYVGL LTAIPILLEP IYEVDITVHA PLLPIVEELM KKRRGSRIY KTIKVAGTPL LEVRGQVPVI ESAGFETDLR LSTNGLGMCQ LYFWHKIWRK VPGDVLDKDA FIPKLKPAPI N SLSRDFVM KTRRRKGIST GGFMSNDGPT LEKYISAELY AQLRENGLVP

UniProtKB: SNU114 isoform 1

Macromolecule #4: BRR2 isoform 1

• Macromolecule: Name: BRR2 isoform 1 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 246.470266 KDa

Sequence

String:

MTEHETKDKA KKIREIYRYD EMSNKVLKVD KRFMNTSQNP QRDAEISQPK SMSGRISAKD MGQGLCNNIN KGLKENDVAV EKTGKSASL KKIQQHNTIL NSSSDFRLHY YPKDPSNVET YEQILQWVTE VLGNDIPHDL IIGTADIFIR QLKENEENED G NIEERKEK IQHELGINID SLKFNELVKL MKNITDYETH PDNSNKQAVA ILADDEKSDE EEVTEMSNNA NVLGGEINDN ED DDEEYDY NDVEVNSKKK NKRALPNIEN DIIKLSDSKT SNIESVPIYS IDEFFLQRKL RSELGYKDTS VIQDLSEKIL NDI ETLEHN PVALEQKLVD LLKFENISLA EFILKNRSTI FWGIRLAKST ENEIPNLIEK MVAKGLNDLV EQYKFRETTH SKRE LDSGD DQPQSSEAKR TKFSNPAIPP VIDLEKIKFD ESSKLMTVTK VSLPEGSFKR VKPQYDEIHI PAPSKPVIDY ELKEI TSLP DWCQEAFPSS ETTSLNPIQS KVFHAAFEGD SNMLICAPTG SGKTNIALLT VLKALSHHYN PKTKKLNLSA FKIVYI APL KALVQEQVRE FQRRLAFLGI KVAELTGDSR LSRKQIDETQ VLVSTPEKWD ITTRNSNNLA IVELVRLLII DEIHLLH DD RGPVLESIVA RTFWASKYGQ EYPRIIGLSA TLPNYEDVGR FLRVPKEGLF YFDSSFRPCP LSQQFCGIKE RNSLKKLK A MNDACYEKVL ESINEGNQII VFVHSRKETS RTATWLKNKF AEENITHKLT KNDAGSKQIL KTEAANVLDP SLRKLIESG IGTHHAGLTR SDRSLSEDLF ADGLLQVLVC TATLAWGVNL PAHTVIIKGT DVYSPEKGSW EQLSPQDVLQ MLGRAGRPRY DTFGEGIII TDQSNVQYYL SVLNQQLPIE SQFVSKLVDN LNAEVVAGNI KCRNDAVNWL AYTYLYVRML ASPMLYKVPD I SSDGQLKK FRESLVHSAL CILKEQELVL YDAENDVIEA TDLGNIASSF YINHASMDVY NRELDEHTTQ IDLFRIFSMS EE FKYVSVR YEEKRELKQL LEKAPIPIRE DIDDPLAKVN VLLQSYFSQL KFEGFALNSD IVFIHQNAGR LLRAMFEICL KRG WGHPTR MLLNLCKSAT TKMWPTNCPL RQFKTCPVEV IKRLEASTVP WGDYLQLETP AEVGRAIRSE KYGKQVYDLL KRFP KMSVT CNAQPITRSV MRFNIEIIAD WIWDMNVHGS LEPFLLMLED TDGDSILYYD VLFITPDIVG HEFTLSFTYE LKQHN QNNL PPNFFLTLIS ENWWHSEFEI PVSFNGFKLP KKFPPPTPLL ENISISTSEL GNDDFSEVFE FKTFNKIQSQ VFESLY NSN DSVFVGSGKG TGKTAMAELA LLNHWRQNKG RAVYINPSGE KIDFLLSDWN KRFSHLAGGK IINKLGNDPS LNLKLLA KS HVLLATPVQF ELLSRRWRQR KNIQSLELMI YDDAHEISQG VYGAVYETLI SRMIFIATQL EKKIRFVCLS NCLANARD F GEWAGMTKSN IYNFSPSERI EPLEINIQSF KDVEHISFNF SMLQMAFEAS AAAAGNRNSS SVFLPSRKDC MEVASAFMK FSKAIEWDML NVEEEQIVPY IEKLTDGHLR APLKHGVGIL YKGMASNDER IVKRLYEYGA VSVLLISKDC SAFACKTDEV IILGTNLYD GAEHKYMPYT INELLEMVGL ASGNDSMAGK VLILTSHNMK AYYKKFLIEP LPTESYLQYI IHDTLNNEIA N SIIQSKQD CVDWFTYSYF YRRIHVNPSY YGVRDTSPHG ISVFLSNLVE TCLNDLVESS FIEIDDTEAE VTAEVNGGDD EA TEIISTL SNGLIASHYG VSFFTIQSFV SSLSNTSTLK NMLYVLSTAV EFESVPLRKG DRALLVKLSK RLPLRFPEHT SSG SVSFKV FLLLQAYFSR LELPVDFQND LKDILEKVVP LINVVVDILS ANGYLNATTA MDLAQMLIQG VWDVDNPLRQ IPHF NNKIL EKCKEINVET VYDIMALEDE ERDEILTLTD SQLAQVAAFV NNYPNVELTY SLNNSDSLIS GVKQKITIQL TRDVE PENL QVTSEKYPFD KLESWWLVLG EVSKKELYAI KKVTLNKETQ QYELEFDTPT SGKHNLTIWC VCDSYLDADK ELSFEI NVK

UniProtKB: UNIPROTKB: A0A6A5Q2I8

Macromolecule #5: Small nuclear ribonucleoprotein Sm D3

• Macromolecule: Name: Small nuclear ribonucleoprotein Sm D3 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 11.240139 KDa

Sequence

String:

MTMNGIPVKL LNEAQGHIVS LELTTGATYR GKLVESEDSM NVQLRDVIAT EPQGAVTHMD QIFVRGSQIK FIVVPDLLKN APLFKKNSS RPMPPIRGPK RR

UniProtKB: Small nuclear ribonucleoprotein Sm D3

Macromolecule #6: BJ4_G0014900.mRNA.1.CDS.1

• Macromolecule: Name: BJ4_G0014900.mRNA.1.CDS.1 / type: protein_or_peptide / ID: 6 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 22.42699 KDa

Sequence

String:

MSKIQVAHSS RLANLIDYKL RVLTQDGRVY IGQLMAFDKH MNLVLNECIE ERVPKTQLDK LRPRKDSKDG TTLNIKVEKR VLGLTILRG EQILSTVVED KPLLSKKERL VRDKKEKKQA QKQTKLRKEK EKKPGKIAKP NTANAKHTSS NSREIAQPSS S RYNGGNDN IGANRSRFNN EAPPQTRKFQ PPPGFKRK

UniProtKB: BJ4_G0014900.mRNA.1.CDS.1

Macromolecule #7: SMD1 isoform 1

• Macromolecule: Name: SMD1 isoform 1 / type: protein_or_peptide / ID: 7 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 16.296798 KDa

Sequence

String:

MKLVNFLKKL RNEQVTIELK NGTTVWGTLQ SVSPQMNAIL TDVKLTLPQP RLNKLNSNGI AMASLYLTGG QQPTASDNIA SLQYINIRG NTIRQIILPD SLNLDSLLVD QKQLNSLRRS GQIANDPSKK RRRDFGAPAN KRPRRGL

UniProtKB: UNIPROTKB: A0A6A5Q0W7

Macromolecule #8: BJ4_G0037700.mRNA.1.CDS.1

• Macromolecule: Name: BJ4_G0037700.mRNA.1.CDS.1 / type: protein_or_peptide / ID: 8 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 12.876066 KDa

Sequence

String:

MSSQIIDRPK HELSRAELEE LEEFEFKHGP MSLINDAMVT RTPVIISLRN NHKIIARVKA FDRHCNMVLE NVKELWTEKK GKNVINRER FISKLFLRGD SVIVVLKTPV E

UniProtKB: Small nuclear ribonucleoprotein Sm D2

Macromolecule #9: Small nuclear ribonucleoprotein E

• Macromolecule: Name: Small nuclear ribonucleoprotein E / type: protein_or_peptide / ID: 9 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 10.385098 KDa

Sequence

String:

MSNKVKTKAM VPPINCIFNF LQQQTPVTIW LFEQIGIRIK GKIVGFDEFM NVVIDEAVEI PVNSADGKED VEKGTPLGKI LLKGDNITL ITSAD

UniProtKB: Small nuclear ribonucleoprotein E

Macromolecule #10: Sm protein F

• Macromolecule: Name: Sm protein F / type: protein_or_peptide / ID: 10 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 9.669945 KDa

Sequence

String:

MSESSDISAM QPVNPKPFLK GLVNHRVGVK LKFNSTEYRG TLVSTDNYFN LQLNEAEEFV AGVSHGTLGE IFIRCNNVLY IRELPN

UniProtKB: Sm protein F

Macromolecule #11: Small nuclear ribonucleoprotein G

• Macromolecule: Name: Small nuclear ribonucleoprotein G / type: protein_or_peptide / ID: 11 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 8.490809 KDa

Sequence

String:

MVSTPELKKY MDKKILLNIN GSRKVAGILR GYDIFLNVVL DDAMEINGED PANNHQLGLQ TVIRGNSIIS LEALDAI

UniProtKB: Small nuclear ribonucleoprotein G

Macromolecule #15: HLJ1_G0053790.mRNA.1.CDS.1

• Macromolecule: Name: HLJ1_G0053790.mRNA.1.CDS.1 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 27.232252 KDa

Sequence

String:

MKFTPSIVID APQYYVDHFN GKYNVDKCVI LRDLQLETDS ESMPSSLKHL TKPTHILDLT NNDLIMIPDL SRRDDIHTLL LGRNNIVEV DGRLLPMNVQ NLTLSNNSIR RFEDLQRLRR APRTLKNLTL IGNQVCHLAN YREHVLRLVP HLETLDFQNV T AEERKSAM SFPRQADGDT LGPVNTAIRD NGSRDKTMEI MNLVVSKMTV ERRNELKKQL AEATSLEEIA RLEKLLSGGV

UniProtKB: U2 small nuclear ribonucleoprotein A'

Macromolecule #16: BJ4_G0027490.mRNA.1.CDS.1

• Macromolecule: Name: BJ4_G0027490.mRNA.1.CDS.1 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 12.850944 KDa

Sequence

String:

MVEPARKKQR IDRDTHHTVA EPVTEAKNTL YVSQLNEKIN MQRLRVNLFL LFATFGEVLK VSMNFKKQRG QAFITMRTID QASLAQISL NGERFFGKPL KVEFSKSETK TL

UniProtKB: MSL1 isoform 1

Macromolecule #17: Small nuclear ribonucleoprotein Sm D3

• Macromolecule: Name: Small nuclear ribonucleoprotein Sm D3 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 8.882204 KDa

Sequence

String:

NGIPVKLLNE AQGHIVSLEL TTGATYRGKL VESEDSMNVQ LRDVIATEPQ GAVTHMDQIF VRGSQIKFIV VPDLLKNAPL F

UniProtKB: Small nuclear ribonucleoprotein Sm D3

Macromolecule #18: PRP9 isoform 1

• Macromolecule: Name: PRP9 isoform 1 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 63.126445 KDa

Sequence

String:

MNLLETRRSL LEEMEIIENA IAERIQRNPE LYYHYIQESS KVFPDTKLPR SSLIAENKIY KFKKVKRKRK QIILQQHEIN IFLRDYQEK QQTFNKINRP EETQEDDKDL PNFERKLQQL EKELKNEDEN FELDINSKKD KYALFSSSSD PSRRTNILSD R ARDLDLNE IFTRDEQYGE YMELEQFHSL WLNVIKRGDC SLLQFLDILE LFLDDEKYLL TPPMDRKNDR YMAFLLKLSK YV ETFFFKS YALLDAAAVE NLIKSDFEHS YCRGSLRSEA KGIYCPFCSR WFKTSSVFES HLVGKIHKKN ESKRRNFVYS EYK LHRYLK YLNDEFSRTR SFVERKLAFT ANERMAEMDI LTQKYEAPAY DSTEKEGAEQ VDGEQRDGQL QEEHLSGKSF DMPL GPDGL PMPYWLYKLH GLDREYRCEI CSNKVYNGRR TFERHFNEER HIYHLRCLGI EPSSVFKGIT KIKEAQELWK NMQGQ SQLT SIAAVPPKPN PSQLKVPTEL ELEEEDEEGN VMSKKVYDEL KKQGLV

UniProtKB: UNIPROTKB: A0A6A5PZ67

Macromolecule #19: Pre-mRNA-splicing factor PRP21

• Macromolecule: Name: Pre-mRNA-splicing factor PRP21 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 33.111512 KDa

Sequence

String:

MEPEDTQLKE DIKTTVNYIK QHGVEFENKL LEDERFSFIK KDDPLHEYYT KLMNEPTDTV SGEDNDRKSE REIARPPDFL FSQYDTGIS RRDMEVIKLT ARYYAKDKSI VEQMISKDGE ARLNFMNSSH PLHKTFTDFV AQYKRVYSFT GQEIKKSKRT I LDNCFERT QYWEFEKDKD REHDKLVELC KIQFAAIPWD KFTQVAKFSI PEDTEIFEGS LDLEQMRLRR VQTGIKLFDS IK PTNEEEK IVSDQGKQKG GDSKGKKRKI RAVGETRLKK SKK

UniProtKB: Pre-mRNA-splicing factor PRP21

Macromolecule #20: PRP11 isoform 1

• Macromolecule: Name: PRP11 isoform 1 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 29.962809 KDa

Sequence

String:

MNYLEGVGSK KGGGGIASES QFNLQRRKEV ESLLSKGENV PYTFQDEKDD QVRSNPYIYK NHSGKLVCKL CNTMHMSWSS VERHLGGKK HGLNVLRRGI SIEKSSLGRE GQTTHDFRQQ QKIIEAKQSL KNNGTIPVCK IATVKNPKNG SVGLAIQVNY S SEVKENSV DSDDKAKVPP LIRIVSGLEL SDTKQKGKKF LVIAYEPFEN IAIELPPNEI LFSENNDMDN NNDGVDELNK KC TFWDAIS KLYYVQFFFK QAEQEQADV

UniProtKB: UNIPROTKB: A0A6A5Q100

Macromolecule #21: HSH155 isoform 1

• Macromolecule: Name: HSH155 isoform 1 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 110.166672 KDa

Sequence

String:

MSHPIQFVNA NNSDKSHQLG GQYSIPQDLR ENLQKEAARI GENEKDVLQE KMETRTVQNR EDSYHKRRFD MKFEPDSDTQ TVTSSENTQ DAVVPRKRKS RWDVKGYEPP DESSTAVKEN SDSALVNVEG IHDLMFFKPS DHKYFADVIS KKPIDELNKD E KKERTLSM LLLKIKNGNT ASRRTSMRIL TDKAVTFGPE MIFNRLLPIL LDRSLEDQER HLMIKTIDRV LYQLGDLTKP YV HKILVVA APLLIDEDPM VRSTGQEIIT NLSTVAGLKT ILTVMRPDIE NEDEYVRNVT SRAAAVVAKA LGVNQLLPFI NAA CHSRKS WKARHTGIKI VQQIGILLGI GVLNHLTGLM SCIKDCLMDD HVPVRIVTAH TLSTLAENSY PYGIEVFNVV LEPL WKGIR SHRGKVLSSF LKAVGSMIPL MDPEYAGYYT TEAMRIIRRE FDSPDDEMKK TILLVLQKCS AVESITPKFL REEIA PEFF QKFWVRRVAL DRPLNKVVTY TTVTLAKKLG CSYTIDKLLT PLRDEAEPFR TMAVHAVTRT VNLLGTADLD ERLETR LID ALLIAFQEQT NSDSIIFKGF GAVTVSLDIR MKPFLAPIVS TILNHLKHKT PLVRQHAADL CAILIPVIKN CHEFEML NK LNIILYESLG EVYPEVLGSI INAMYCITSV MDLDKLQPPI NQILPTLTPI LRNKHRKVEV NTIKFVGLIG KLAPTYAP P KEWMRICFEL LELLKSTNKE IRRSANATFG FIAEAIGPHD VLVALLNNLK VQERQLRVCT AVAIGIVAKV CGPYNVLPV IMNEYTTPET NVQNGVLKAM SFMFEYIGNM SKDYIYFITP LLEDALTDRD LVHRQTASNV ITHLALNCSG TGHEDAFIHL MNLLIPNIF ETSPHAIMRI LEGLEALSQA LGPGLFMNYI WAGLFHPAKN VRKAFWRVYN NMYVMYQDAM VPFYPVTPDN N EEYIEELD LVL

UniProtKB: UNIPROTKB: A0A6A5PSM1

Macromolecule #22: HLJ1_G0043010.mRNA.1.CDS.1

• Macromolecule: Name: HLJ1_G0043010.mRNA.1.CDS.1 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 27.503838 KDa

Sequence

String:

KPSLSQLKSQ VPYPQIIEWY DCDARYPGLL ASIKCTKNVI PVPSHWQSKK EYLSGRSLLG KRPFELPDII KKTNIEQMRS TLPQGGLDG QDEKSLKEAS RARVQPKMGA LDLDYKKLHD VFFKIGANWK PDHLLCFGDV YYENRNLFEE TNWKRMVDHK R PGRISQEL RAIMNLPEGQ LPPWCMKMKD IGLPTGYPDL KIAGLNWDIT NLKGDVYGKI IPNHHSRPKK QDRNYFGALI

UniProtKB: HLJ1_G0043010.mRNA.1.CDS.1

Macromolecule #23: RSE1 isoform 1

• Macromolecule: Name: RSE1 isoform 1 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 153.956781 KDa

Sequence

String:

MWGGGKMAVV SLSPHTAKMR KLFGQASTTM AYDGLKREAE RRTRSDHNIT MVAKDDELYL YHLTLKKQTN FVHSCIGHFV DLEAGSKRE QSQLCVATET HLELYDTADG ELKLIAKFQN LFATITSMKS LDLPHSGSRA KASNWPTFLA LTSDSGNLSI V QIIMHAGA LRLKTLVNQP LTRTTLRRVS PISYMEIDPN GRCIILSSVE QNKLCFLVDY AQKLRISSPL EIIRPHMVTL DM AVVDVNF NNPCFVTLEI DNAATQLSVH LIFYVLELGL NHIVKKADYL VNPSANFVLS LPDLSRYNIT TSLSDNNYDA DYD TLFNPF VVIGFENHIL VKDMNGFFSL KVEIPKRSIT NSRHKNVTII SGIVQKLKND FFVLLQSNHG DLFKLTVSPD TNDR NRPLV QLSYFDTIQN SHQLHIFKNG YLFALSEMNN NFLFQFEKLG VEKNDFSNVL TSKDPNKSLV FEPSIKLQNL SILSQ QLNL NPSIKSQIVS DSPLSIATKH FTNNKIITLT NAVNYSNLIS TSLPPNATKL WLIPDPATTG DNNTLLFITF PKKTMI LQI DNESMEELTP DEATRSAFKL SQDTTIHTCL MGSHSIIQVC TAELRHIVPT GKSRYSNKLT WVPPAGIRIV CATSSKT QL IISLSNYELV YFKIDVSSDS LIELTTHPEL DTMPSKVAIV QDTQHADLLA IADNEGMIKI MSLKDQKEDF LTVISLQL V SEKISDMIMV RDSSIGQLNL HVGLENGVYM KFHIGDVDGS FTDIKRRFLG LKPVSLSYLR EISVSLNNEE EEEEEEDDD DEKEEEEINS SGAKWMSCVV CHSSSTWVSY TWKNVWTIRQ LKDQNMLSCS KFVNADVAIN GVCSISSSGR LNIGRVSNFP TLDNWFHVH ESSVNKQENG GGDESNEEEE DEMEEEMEML QISTFRPRTI LSFPNNPKSI LFIDNHSGKK QCRISLQIDG E CLKFGSSD HLYKILDDID CVSAAIIDFT RQADHLIICA GDKRLLTYKI LVNKDKLSFD IELLHQTEII SPIHAMLKFK NF LLTAMGS TIVLYGLGKK QLLRRSVTQT PVSITKIVSM HQWNYERLAV GDIHESVTLF IWDPAGNVFI PYVDDSVKRH VTV LKFLDE ATVIGADRYG NAWTLRSPPE CEKIMSNHDP SELSNGAIKY PLDVITLQQK LPNTYDCKFK FQLLNHFFVN DIIT DFHIL DSLSNSDRPG CIYMGLQGTV GCFIPLLSKG NVFMMGNIEN IMAEADDTFY LDYESRKKNN NMRKEDDEEE SGSVV LQGR HGIEDEIICE GSCSILGRDH QEYRSYYAPV RKVIDGDLCE NFLRLSLNEQ EFLAKNLKSV QVEDIIQTIN EVRTNY M

UniProtKB: UNIPROTKB: A0A6A5PUP5

Macromolecule #24: HSH49 isoform 1

• Macromolecule: Name: HSH49 isoform 1 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 24.534152 KDa

Sequence

String:

MNYSADSGNT VYVGNIDPRI TKEQLYELFI QINPVLRIKY PKDKVLQAYQ GYAFIEFYNQ GDAQYAIKIM NNTVRLYDRL IKVRQVTNS TGTTNLPSNI SKDMILPIAK LFIKNLADSI DSDQLVKIFN KFGKLIREPE IFYLSNGKLK CAYVYFEDFE K ADLAIKSL NNQLVANNRI TVDYAFKENG KGNAKYGDDV DRLLNKEALK HNMLK

UniProtKB: UNIPROTKB: A0A6A5Q466

Macromolecule #25: BJ4_G0056610.mRNA.1.CDS.1

• Macromolecule: Name: BJ4_G0056610.mRNA.1.CDS.1 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 12.283573 KDa

Sequence

String:

MSRHQFDLIM CLKQPGVQTG LLCEKCDGKC PICDSYVRPK RKVRVCENCS FGKQAKNCII CNLNVGVNDA FYCWECCRLG KDKDGCPRI LNLGSNRLDR HFEKKKKV

UniProtKB: RDS3 isoform 1

Macromolecule #26: RDS3 complex subunit 10

• Macromolecule: Name: RDS3 complex subunit 10 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 9.888207 KDa

Sequence

String:

MAEKQRQLKL QKIYKQKYIG LGDESTTREQ WQRNVRNDTL NTLQGHSASL EYVSLSRGDL SIRDTRIHLL KSMSPGYKAY LREE

UniProtKB: RDS3 complex subunit 10

Macromolecule #27: Pre-mRNA-splicing factor CEF1

• Macromolecule: Name: Pre-mRNA-splicing factor CEF1 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 67.837773 KDa

Sequence

String:

MPPVPIYVKG GVWTNVEDQI LKAAVQKYGT HQWSKVASLL QKKTARQSEL RWNEYLNPKL NFTEFSKEED AQLLDLAREL PNQWRTIAD MMARPAQVCV ERYNRLLESE DSGGAALSTG VTDLKAGDIN PNAETQMARP DNGDLEDEEK EMLAEARARL L NTQGKKAT RKIRERMLEE SKRIAELQKR RELKQAGINV AIKKPKKKYG TDIDYNEDIV YEQAPMPGIY DTSTEDRQIK KK FEQFERK VNRKGLDGNK DKPSKKNKDK KRKHDENEHV EKAALGESTT LTDEYKKPKL ILSAPGTKQG KVTYKKKLES KRQ KLIEAQ ATGTVLTPKE LLPHDSGQED NERSNIKSGK QLKSRIRKFL VQMFASLPSP KNDFEIVLSE DEKEEDAEIA EYEK EFENE RAMNEEDNFI EPPSQNDAPR VSLVAVPLAY STLPIPEFKN NPQSAIDNKY NLLVANAINK EPHMVPEDTV DFLKE VESR MQHITQGRTS MKIQFKTAMP PTEVLLESIQ SKVESIEQLQ RKLQHVQPLE QQNNEMCSTL CHHSLPALIE GQRKYY ADY YAYRQEIRSL EGRRKRLQAM LNSSSSI

UniProtKB: Pre-mRNA-splicing factor CEF1

Macromolecule #28: Pre-mRNA-processing factor 19

• Macromolecule: Name: Pre-mRNA-processing factor 19 / type: protein_or_peptide / ID: 28 / Number of copies: 4 / Enantiomer: LEVO / EC number: RING-type E3 ubiquitin transferase
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 56.629777 KDa

Sequence

String:

MLCAISGKVP RRPVLSPKSR TIFEKSLLEQ YVKDTGNDPI TNEPLSIEEI VEIVPSAQQA SLTESTNSAT LKANYSIPNL LTSLQNEWD AIMLENFKLR STLDSLTKKL STVMYERDAA KLVAAQLLME KNEDSKDLPK SSQQAVAITR EEFLQGLLQS S RDFVARGK LKAPKWPILK NLELLQAQNY SRNIKTFPYK ELNKSMYYDK WVCMCRCEDG ALHFTQLKDS KTITTITTPN PR TGGEHPA IISRGPCNRL LLLYPGNQIT ILDSKTNKVL REIEVDSANE IIYMYGHNEV NTEYFIWADN RGTIGFQSYE DDS QYIVHS AKSDVEYSSG VLHKDSLLLA LYSPDGILDV YNLSSPDQAS SRFPVDEEAK IKEVKFADNG YWMVVECDQT VVCF DLRKD VGTLAYPTYT IPEFKTGTVT YDIDDSGKNM IAYSNESNSL TIYKFDKKTK NWTKDEESAL CLQSDTADFT DMDVV CGDG GIAAILKTND SFNIVALTP

UniProtKB: UNIPROTKB: A0A6A5PQI0

Macromolecule #29: SNT309 isoform 1

• Macromolecule: Name: SNT309 isoform 1 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 20.741455 KDa

Sequence

String:

MDGLSFVDKG KIPDGYKNEI DQLVKKEFAN IKREPVHPEI RGILAKRKGA DNSVSTLTNA LYTEYLKQRN NKKRRTPDFN DDDDTLFLE EYRRKYPRID TSRYIPNESS EVSLLGIVDS YLKHQEIVLD TLLPQTVSNQ WRINNDYIRQ TCTIVEEMNI Q QRKQINDL EIYRKRL

UniProtKB: UNIPROTKB: A0A6A5Q641

Macromolecule #30: BUD31 isoform 1

• Macromolecule: Name: BUD31 isoform 1 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 18.484502 KDa

Sequence

String:

MPRIKTRRSK PAPDGFEKIK PTLTDFEIQL RDAQKDKSSK LAAKSNEQLW EIMQLHHQRS RYIYTLYYKR KAISKDLYDW LIKEKYADK LLIAKWRKTG YEKLCCLRCI QKNETNNGST CICRVPRAQL EEEARKKGTQ VSFHQCVHCG CRGCASTD

UniProtKB: BUD31 isoform 1

Macromolecule #31: HLJ1_G0054350.mRNA.1.CDS.1

• Macromolecule: Name: HLJ1_G0054350.mRNA.1.CDS.1 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 37.639734 KDa

Sequence

String:

IVNRYEKLLS QRPEWHAPWK LSRVINGHLG WVRCVAIDPV DNEWFITGSN DTTMKVWDLA TGKLKTTLAG HVMTVRDVAV SDRHPYLFS VSEDKTVKCW DLEKNQIIRD YYGHLSGVRT VSIHPTLDLI ATAGRDSVIK LWDMRTRIPV ITLVGHKGPI N QVQCTPVD PQVVSSSTDA TVRLWDVVAG KTMKVLTHHK RSVRATALHP KEFSVASACT DDIRSWGLAE GSLLTNFESE KT GIINTLS INQDDVLFAG GDNGVLSFYD YKSGHKYQSL ATREMVGSLE GERSVLCSTF DKTGLRLITG EADKSIKIWK QDE TATKES EPGLAWNPNL S

UniProtKB: HLJ1_G0022770.mRNA.1.CDS.1

Macromolecule #32: Pre-mRNA-processing protein 45

• Macromolecule: Name: Pre-mRNA-processing protein 45 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 42.548727 KDa

Sequence

String:

MFSNRLPPPK HSQGRVSTAL SSDRVEPAIL TDQIAKNVKL DDFIPKRQSN FELSVPLPTK AEIQECTART KSYIQRLVNA KLANSNNRA SSRYVTETHQ APANLLLNNS HHIEVVSKQM DPLLPRFVGK KARKVVAPTE NDEVVPVLHM DGSNDRGEAD P NEWKIPAA VSNWKNPNGY TVALERRVGK ALDNENNTIN DGFMKLSEAL ENADKKARQE IRSKMELKRL AMEQEMLAKE SK LKELSQR ARYHNGTPQT GAIVKPKKQT STVARLKELA YSQGRDVSEK IILGAAKRSE QPDLQYDSRF FTRGANASAK RHE DQVYDN PLFVQQDIES IYKTNYEKLD EAVNVKSEGA SGSHGPIQFT KAESDDKSDN YGA

UniProtKB: UNIPROTKB: A0A6A5PTY9

Macromolecule #33: Pre-mRNA-splicing factor SLT11

• Macromolecule: Name: Pre-mRNA-splicing factor SLT11 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 40.98859 KDa

Sequence

String:

MNDEINEPPP NICEQCLGDE ANIRMTKIPQ GSECKICTLP FTLYHFKTSK RSNNIIKTLI CVRCATQRNI CQCCMLDSRW HIPIQLRDH LISLVNEENV MTEEAKNDMM KRFLSLKNVK LGGAQITSDP SEADNIVDKL KNILLRATSD GPSTPLIKNT T ALYKNEKG ANEVKNLEKY ASVDISHILK KLPLNESFLK NPSTKSFFLY NIDASIPEWK ITDTVSQLLG IKKWKDGNSL SL IVNHKAK CGGLRFQSSE LGERFVSKIS ETLVTPKGLK RGVLLIDRFR IFIIPWSSGF SAASFGTNTA ENIKLSLSLN KLI QLELGL SFPTKSTDNA KNDKKKTSKK VHKDRSKKSK PRANKLTI

UniProtKB: UNIPROTKB: A0A6A5Q0Z2

Macromolecule #34: Pre-mRNA-splicing factor CWC2

• Macromolecule: Name: Pre-mRNA-splicing factor CWC2 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 29.742814 KDa

Sequence

String:

MTSWRDKSAK VQVKESELPS SIPAQTGLTF NIWYNKWSQG FAGNTRFVSP FALQPQLHSG KTRGDNDGQL FFCLFFAKGM CCLGPKCEY LHHIPDEEDI GKLALRTEVL DCFGREKFAD YREDMGGIGS FRKKNKTLYV GGIDGALNSK HLKPAQIESR I RFVFSRLG DIDRIRYVES KNCGFVKFKY QANAEFAKEA MSNQTLLLPS DKEWDDRREG TGLLVKWANE DPDPAAQKRL QE ELKLESL NMMVHLINNN TNSA

UniProtKB: Pre-mRNA-splicing factor CWC2

Macromolecule #35: Pre-mRNA-splicing factor CWC15

• Macromolecule: Name: Pre-mRNA-splicing factor CWC15 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 19.975195 KDa

Sequence

String:

MTTSHRPQLE ARSGAKAAAY TPTGIEHARL LPGHTTLKYR KFKEEENLRA NCAQEDRSND KSLEEAVMNE EKQDVVGSGN LQETRSEKD QKDSLQELLV TQKNKVEDKA ELEGNEQLKG GNSSRRSWRK GTAFGRHKVT KETNIKEHAT KKSASGYIND M TKSEYHQE FLHKHVR

UniProtKB: Pre-mRNA-splicing factor CWC15

Macromolecule #36: Pre-mRNA leakage protein 1

• Macromolecule: Name: Pre-mRNA leakage protein 1 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 23.685682 KDa

Sequence

String:

MFHRRKRPYN TRNYGHDDKK FKSQYIDIMP DFSPSGLLEL ESNNKEGIAL KHVEPQDAIS PDNYMDMLGL EARDRTMYEL VIYRKNDKD KGPWKRYDLN GRSCYLVGRE LGHSLDTDLD DRTEIVVADI GIPEETSSKQ HCVIQFRNVR GILKCYVMDL D SSNGTCLN NVVIPGARYI ELRSGDVLTL SEFEEDNDYE LIFMNV

UniProtKB: Pre-mRNA leakage protein 1

Macromolecule #37: SX2_G0027210.mRNA.1.CDS.1

• Macromolecule: Name: SX2_G0027210.mRNA.1.CDS.1 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 14.880456 KDa

Sequence

String:

NKIQQINDKE LQSGILSPHQ SWHNEYKDNA YIYIGNLNRE LTEGDILTVF SEYGVPVDVI LSRDENTGES QGFAYLKYED QRSTILAVD NLNGFKIGGR ALKIDHTFYR PKRSLQKYYE AVKEELDRD

UniProtKB: UNIPROTKB: A0A6L1ALU2

Macromolecule #38: Pre-mRNA-splicing factor CWC26

• Macromolecule: Name: Pre-mRNA-splicing factor CWC26 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 14.039636 KDa

Sequence

String:

SKYEDEKAAE REQYLKNLNM GDVQKLGINV DAHDKKKNQT ASSLTIEDPA ITFTHDKERT VKTSLLGRKL YDKPAPENRF AIMPGSRWD GVHRSNGFEE KWFAKQNEIN EKKVQSYTLQ ED

UniProtKB: UNIPROTKB: A0A6L1AYM3

Macromolecule #39: CDC40 isoform 1

• Macromolecule: Name: CDC40 isoform 1 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 52.128762 KDa

Sequence

String:

MGLVDGYDTS SDSDLNFDEG KSVHEKKNGN LHEDTSYEPS SNNIHKRKSH FTKSELKRRR KTRKGDGPWG SWSSSDDETS QASETQKED QDIFVHALAE DNLDSEQIEV EEVSHFYGKS EKDYQGRGYL YPPNDVDVDL REERISFRCY LPKKVIRNYP G HPEGTTAL KFLPKTGHLI LSGGNDHTIK IWDFYHDYEC LRDFQGHNKP IKALRFTEDC QSFLSSSFDR SVKIWDTETG KV KTRLHLN STPADVESRP TNPHEFIVGL SNSKILHYDD RVSENQGLVQ TYDHHLSSIL ALKYFPDGSK FISSSEDKTV RIW ENQINV PIKQISDTAQ HSMPFLNVHP SQNYFCAQSM DNRIYSFSLK PKYKRHPKKI FKGHSSAGYG ISLAFSGDGR YICS GDSKS RLFTWDWNTS RLLNNIKIPG NKPITQVDWH PQETSKVICS GAAGKIYVCD

UniProtKB: UNIPROTKB: A0A6A5PYD5

Macromolecule #40: Pre-mRNA-splicing factor CWC21

• Macromolecule: Name: Pre-mRNA-splicing factor CWC21 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 15.793596 KDa

Sequence

String:

MSYNGIGLKS AKGSSTSGHV QRSLASNNRR RPQGSQQQRQ QRQNAIKKAS HDKASRPLAV QKQIETHMEK REIEVQVSEL RDRLEEEET LSEEQIDKKC EALRAKLTNE WQEQQRMSSL YTPRKARLTE EQHRHE

UniProtKB: Pre-mRNA-splicing factor CWC21

Macromolecule #41: CWC22 isoform 1

• Macromolecule: Name: CWC22 isoform 1 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 67.386062 KDa

Sequence

String:

MSTATIQDED IKFQRENWEM IRSHVSPIIS NLTMDNLQES HRDLFQVNIL IGRNIICKNV VDFTLNKQNG RLIPALSALI ALLNSDIPD IGETLAKELM LMFVQQFNRK DYVSCGNILQ CLSILFLYDV IHEIVILQIL LLLLEKNSLR LVIAVMKICG W KLALVSKK THDMIWEKLR YILQTQELSS TLRESLETLF EIRQKDYKSG SQGLFILDPT SYTVHTHSYI VSDEDEANKE LG NFEKCEN FNELTMAFDT LRQKLLINNT SDTNEGSNSQ LQIYDMTSTN DVEFKKKIYL VLKSSLSGDE AAHKLLKLKI ANN LKKSVV DIIIKSSLQE STFSKFYSIL SERMITFHRS WQTAYNETFE QNYTQDIEDY ETDQLRILGK FWGHLISYEF LPMD CLKII KLTEEESCPQ GRIFIKFLFQ ELVNELGLDE LQLRLNSSKL DGMFPLEGDA EHIRYSINFF TAIGLGLLTE DMRSR LTII QEVEDAEEEE KKLREEEELE KLRKKARESQ PTQGPKIHES RLFLQKDTRE NSRSRSPFTV ETRKRARSRT PPRGSR NHR NRSRTPPARR QRHR

UniProtKB: UNIPROTKB: A0A6A5Q1E9

Macromolecule #42: Pre-mRNA-splicing factor CWC24

• Macromolecule: Name: Pre-mRNA-splicing factor CWC24 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 29.797941 KDa

Sequence

String:

MFRKRLVNKS SSDEKNQKKR QKINFSEEKL VASDEEKGSS DLMSLAKSGN SRTLQLSHEN EGKLQKKGED LDKYTLTVND DSTKEDLLN FERKELAEKA KKRRPSDDNE LVLNMSGKNK RLTKQINQPT NIRTTVLMDF QPDVCKDYKQ TGYCGYGDSC K FLHSRDDF KTGWKLNQEW NADKEDSKAV TLDLEKIPFK CTLCKEDYKS PVVTNCGHYF CGSCFAKDMK KGTKCFICHK ET HGSAKVA SDLQKMLNKR KS

UniProtKB: Pre-mRNA-splicing factor CWC24

Macromolecule #43: CWC27 isoform 1

• Macromolecule: Name: CWC27 isoform 1 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 35.08093 KDa

Sequence

String:

MSSNIEPQTT AKCILYTTKG NIAIELWAKE CPETCKRFLS MLSDGTFTNG EFKELKPTQW LMFNANSTGE YRTVAEEKNP RIRFNRDGL LGWDRRRNTW FITVLADSKH VLNDCNVFGK IVGKSIYIFR EILGGEIEAS SRDNDVKRFM YPAVLKDVEI T IPFFEDIF GSKRRLEDNE KKEQEPAKKL VKSAKVKMVY EDEQEDDDGD VQKLKPRKRM ILPAWIKDDS RSEGIKLDAS LD QPQEALI REKTELHDNV DEATTKETES QENIKEEPMD KRERETLAML SKFQERIKNK NILK

UniProtKB: UNIPROTKB: A0A6A5Q291

Macromolecule #44: Pre-mRNA-splicing factor SPP2

• Macromolecule: Name: Pre-mRNA-splicing factor SPP2 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 20.685377 KDa

Sequence

String:

MSKFSLKLGS KTLKKNISKK TKKKNSLQKA NLFDWDDAET ASLSHKPQSK IKIQSIDKFD LDEESSSKKK LVIKLSENAD TKKNDAPLV EYVTEKEYNE VPVEEFGDAL LRGMGWESDS EQDSKGDKTQ SRNKDVSNVS QIHPDGLGIG AKLNKAINVE E ASFMPVVK IDKITGTKVD DDKKNKS

UniProtKB: Pre-mRNA-splicing factor SPP2

Macromolecule #45: CLF1 isoform 1

• Macromolecule: Name: CLF1 isoform 1 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 82.555859 KDa

Sequence

String:

MDTLEPTAVD THVSAEQILR DVYKKGQKAR GSTNIDILDL EELREYQRRK RTEYEGYLKR NRLDMGQWIR YAQFEIEQHD MRRARSIFE RALLVDSSFI PLWIRYIDAE LKVKCINHAR NLMNRAISTL PRVDKLWYKY LIVEESLNNV EIVRSLYTKW C SLEPGVNA WNSFVDFEIR QKNWNGVREI YSKYVMAHPQ MQTWLKWVRF ENRHGNTEFT RSVYSLAIDT VANLQNLQIW SD MEVAKLV NSFAHWEAAQ QEYERSSALY QIAIEKWPSN QLLKAGLLDF EKQFGDINSI EETISYKRKM EYETILSNNA YDY DTWWLY LDLISESFPK QIMQTFEKAI VDSRPKELSK NVQWKRYIYL WMRYICYVEL ELENSLLEEE LFQRLIDDII PHKH FTFSK IWLMYAKFLI RHDDVPKARK ILGKAIGLCP KAKTFKGYIE LEVKLKEFDR VRKIYEKFIE FQPSDLQIWS QYGEL EENL GDWDRVRGIY TIALDENSDF LTKEAKIVLL QKYITFETES QEFEKARKLY RRYLELNQYS PQSWIEFAMY QTSTPT EQQ LLDLAKLQSE NVDEDIEFEI TDENKLEARK VFEEAIVFFK EKDDKQGRLS ILEALKDYEE TYGTELDQET VKKRFPK VI KKVRLQNGVE EEFVDYIFPD DIDDDKPKPS KFLELAKKWK QEQAL

UniProtKB: Pre-mRNA-splicing factor CLF1

Macromolecule #46: SYF1 isoform 1

• Macromolecule: Name: SYF1 isoform 1 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 100.344016 KDa

Sequence

String:

MSAYIAMKGV ITNVDENIRN DEDVAFEYEI QKTPQNILTW KRYIEYWKEE GRTDKQIRWL YERFCSQFVT DTSIWEDYIR WESTKEVVE TSRIFWLFQR CLKSCVRDCD RICLSYLELA IEQYDLAMIR HALASSLMKM EREMHRKVWD PVIKFVEEKV L PLTQLDST QEDEEESTDE AELINVLLVK GFTKGGFISE EISENGSRGD IWSSHILERY LKVAPQQKRN ESLATLALTR DN ITIKSVY EKYLPQDENS GKYLPSSELP FELNFNYLAS LEKLGLDNQY EEFMRQMNGI YPDKWLFLIL SLAKYYISRG RLD SCGDLL KKSLQQTLRY SDFDRIYNFY LLFEQECSQF ILGKLKENDS KFFNQKDWTE KLQAHMATFE SLINLYDIYL NDVA LRQDS NLVETWMKRV SLQKSAAEKC NVYSEAILKI DPRKVGTPGS FGRLWCSYGD LYWRSNAIST ARELWTQSLK VPYPY IEDL EEIYLNWADR ELDKEGVERA FSILEDALHV PTNPEILLEK YKNGHRKIPA QTVLFNSLRI WSKYIDYLEA YCPKDA NSS DKIFNKTKMA YNTVIDLRLI TPAMAENFAL FLQNHYEVME SFQVYEKTIP LFPPEIQYEL WIEYLEVATS HQLSSLS PE HIRFLFEKAL KNLCSNGIDC KTIFIAYSVF EERISGLISK SIEILRRGAV IGTVSVSTHL ESRLQLWRMC ISKAESTL G PSVTRELYQE CIQILPNSKA VEFVIKFSDF ESSIGETIRA REILAYGAKL LPPSRNTELW DSFEIFELKH GDKETYKDM LKMKKVLESN MLIDSASVSH EEGNINFVAA ATSHAPNSHT LTQSTSSYSI NPDEIELDI

UniProtKB: UNIPROTKB: A0A6A5Q0A4

Macromolecule #47: Pre-mRNA-splicing factor ATP-dependent RNA helicase-like protein PRP2

• Macromolecule: Name: Pre-mRNA-splicing factor ATP-dependent RNA helicase-like protein PRP2; type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO / EC number: RNA helicase
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 99.947492 KDa

Sequence

String:

MSSITSETGK RRVKRTYEVT RQNDNAVRIE PSSLGEEEDK EAKDKNSALQ LKRSRYDPNK VFSNTNQGPE KNNLKGEQLG SQKKSSKYD EKITSNNELT TKKGLLGDSE NETKYASSNS KFNVEVTHKI KNAKEIDKIN RQRMWEEQQL RNAMAGQSDH P DDITLEGS DKYDYVFDTD AMIDYTNEED DLLPEEKLQY EARLAQALET EEKRILTIQE ARKLLPVHQY KDELLQEIKK NQ VLIIMGE TGSGKTTQLP QYLVEDGFTD QGKLQIAITQ PRRVAATSVA ARVADEMNVV LGKEVGYQIR FEDKTTPNKT VLK YMTDGM LLREFLTDSK LSKYSCIMID EAHERTLATD ILIGLLKDIL PQRPTLKLLI SSATMNAKKF SEFFDNCPIF NVPG RRYPV DIHYTLQPEA NYIHAAITTI FQIHTTQSLP GDILVFLTGQ EEIERTKTKL EEIMSKLGSR TKQMIITPIY ANLPQ EQQL KIFQPTPENC RKVVLATNIA ETSLTIDGIR YVIDPGFVKE NSYVPSTGMT QLLTVPCSRA SVDQRAGRAG RVGPGK CFR IFTKWSYLHE LELMPKPEIT RTNLSNTVLL LLSLGVTDLI KFPLMDKPSI PTLRKSLENL YILGALNSKG TITRLGK MM CEFPCEPEFA KVLYTAATHE QCQGVLEECL TIVSMLHETP SLFIGQKRDA AASVLSEVES DHILYLEIFN QWRNSKFS R SWCQDHKIQF KTMLRVRNIR NQLFRCSEKV GLVEKNDQAR MKIGNIAGYI NARITRCFIS GFPMNIVQLG PTGYQTMGR SSGGLNVSVH PTSILFVNHK EKAQRPSKYV LYQQLMLTSK EFIRDCLVIP KEEWLIDMVP QIFKDLIDDK TNRGRR

UniProtKB: Pre-mRNA-splicing factor ATP-dependent RNA helicase-like protein PRP2

Macromolecule #2: U5 snRNA

• Macromolecule: Name: U5 snRNA / type: rna / ID: 2 / Number of copies: 1
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 68.643344 KDa

Sequence

String:

AAGCAGCUUU ACAGAUCAAU GGCGGAGGGA GGUCAACAUC AAGAACUGUG GGCCUUUUAU UGCCUAUAGA ACUUAUAACG AACAUGGUU CUUGCCUUUU ACCAGAACCA UCCGGGUGUU GUCUCCAUAG AAACAGGUAA AGCUGUCCGU UACUGUGGGC U UGCCAUAU UUUUUGGAAC UUUUCUGCCC UUUUUCUCAA UGAGUAAGGA GGGCGU

GENBANK: GENBANK: CP008004.1

Macromolecule #12: U6 snRNA

• Macromolecule: Name: U6 snRNA / type: rna / ID: 12 / Number of copies: 1
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 35.883176 KDa

Sequence

String:

GUUCGCGAAG UAACCCUUCG UGGACAUUUG GUCAAUUUGA AACAAUACAG AGAUGAUCAG CAGUUCCCCU GCAUAAGGAU GAACCGUUU UACAAAGAGA UUUAUUUCGU UUU

GENBANK: GENBANK: CP007856.1

Macromolecule #13: pre-mRNA

• Macromolecule: Name: pre-mRNA / type: rna / ID: 13 / Number of copies: 1
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 44.772172 KDa

Sequence

String:

AAAAUAAAAA AAAAAAAAUU UGUAAGGUAU GUAUUAUUUU UU(N)(N)(N)(N)(N)(N)(N)(N) (N)(N)(N)(N) (N)(N)(N)(N)(N) (N)(N)(N)(N)(N)(N)(N)(N)(N)(N) (N)(N)(N)(N)(N)(N)(N)(N)(N)(N) (N) (N)(N)(N)(N)(N)(N)(N)(N) (N)(N)(N)(N)AAAAAA AA(N)(N)(N)AAAAA A(N)AAAAACUA GAUACUAACA CAUUUAAUU UUUUUUUGUU UUU(N)(N)UUUUU UUUUU

Macromolecule #14: U2 snRNA

• Macromolecule: Name: U2 snRNA / type: rna / ID: 14 / Number of copies: 1
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 376.324562 KDa

Sequence

String:

ACGAAUCUCU UUGCCUUUUG GCUUAGAUCA AGUGUAGUAU CUGUUCUUUU CAGUGUAACA ACUGAAAUGA CCUCAAUGAG GCUCAUUAC CUUUUAAUUU GUUACAAUAC ACAUUUUUUG GCACCCAAAA UAAUAAAAUG GACGGGAAGA GACUUUUUAA G CAAGACGU UUUCCGCUAA UGACACCUCG CACGAGUCGU UCUUGCUAUC UUUGGUCGCU UGAUGUUUCU UCUUAACCCG UU CUUAUGA UGGUUUUUCG AAAUUGGUUU UUGAGACGAC GGUUGCUCAA GGUUAUUGUU UUUGUUUUCU UCUGGUUGUU UUC UAUUUU CUUUUUUUUA GCUUUCUGUU UCUCCCUUAG UUUGGCUUUU UGCUUCAUAC UCUUCCCUGU CUUUCCGAGC CGUU UAUGU CCAACGCGGG AUUUGGUUUU UCUUUAUCGA UGGGAAGAAA UGGUGCUAUA GUAGGUUGGG AGAUAAUAUU UAUGG UAUG GGGUGCUAGU GCGGAUGGGG CGCUCUUAUU GUUGAUUUCU UCGCUCGUCU UCUUUUUCUG GUGGCGCUGC AAGAGG AAG UUUUUCGACU UUGUUAUGAU UUUUGGUUUG CAAGGAAAGG UGUCUUACGA UUCUUUUUUU GAUGUAAUAG GAUAAGC UU GCUUAUCCCC CAAGUAUCGG CCAAAGUUGU UGAUUUUCCU UUUGAAGUGU CCUCGGUUUG AGGGGGUGUA GGGUGGGG U UGGUCUACAA UAAGAGUGUU CCAUUGUUAA CGUGCUGGCG UCUUUUACUA UAUUUUUUUU CCCAGUUUAU UUUGUGCUU AUUUUCUCAU UGAGGAGAAG GAGCUCUUCU CGCAGGAUAU AAAUGGAGGU UUGCUAAAGG GGAGGAGAUG UGUUUGUGAG AAUACUGCU GAGAGAGUUC UGGAAGAGAA AAAAAGGAGG CAAUGGAAGG CGUUUGCUGG GAAAAGAGAA GAGCCAUGAC U GCAUCUGU UGUUUCAAGG CCAGUUUUAU UAACCGCCUA UGUCAUAGAG GCGUUUUUUU UGGAGGGAUU UGAAGAAUGC CG GCGGCAU CAAGAAACGG ACUUGAUGGU UGACGCCUGU UUUUAAAGUU AGAGACGUCG CGACCCUCGC ACUUGUGGAG UCG UUCUUG ACUUUUACUU UGGUCGCUUG AUGUUUCUCU CGUCUUCCCG UUCGCUCUU

Macromolecule #48: INOSITOL HEXAKISPHOSPHATE

• Macromolecule: Name: INOSITOL HEXAKISPHOSPHATE / type: ligand / ID: 48 / Number of copies: 1 / Formula: IHP
• Molecular weight: Theoretical: 660.035 Da

Chemical component information

ChemComp-IHP:
INOSITOL HEXAKISPHOSPHATE / Phytic acid

Macromolecule #49: GUANOSINE-5'-TRIPHOSPHATE

• Macromolecule: Name: GUANOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 49 / Number of copies: 1 / Formula: GTP
• Molecular weight: Theoretical: 523.18 Da

Chemical component information

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM / Guanosine triphosphate

Macromolecule #50: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 50 / Number of copies: 6 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Macromolecule #51: CALCIUM ION

• Macromolecule: Name: CALCIUM ION / type: ligand / ID: 51 / Number of copies: 1 / Formula: CA
• Molecular weight: Theoretical: 40.078 Da

Macromolecule #52: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 52 / Number of copies: 15 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Macromolecule #53: water

• Macromolecule: Name: water / type: ligand / ID: 53 / Number of copies: 1714 / Formula: HOH
• Molecular weight: Theoretical: 18.015 Da

Chemical component information

ChemComp-HOH:
WATER / Water

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Buffer: pH: 7.9
• Grid: Model: Quantifoil / Material: GOLD / Support film - #0 - Film type ID: 1 / Support film - #0 - Material: GRAPHENE / Support film - #0 - topology: CONTINUOUS / Support film - #1 - Film type ID: 2 / Support film - #1 - Material: CARBON / Support film - #1 - topology: CONTINUOUS / Support film - #2 - Film type ID: 3 / Support film - #2 - topology: HOLEY
• Vitrification: Cryogen name: ETHANE

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy
• Image recording: Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: SUPER-RESOLUTION / Average electron dose: 50.0 e/Ų
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• Startup model: Type of model: EMDB MAP
• Initial angle assignment: Type: MAXIMUM LIKELIHOOD
• Final angle assignment: Type: MAXIMUM LIKELIHOOD
• Final reconstruction: Resolution.type: BY AUTHOR / Resolution: 2.5 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 705371