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Yorodumi- EMDB-30553: Cryo-EM structure of disome from saccharomyces cerevisiae, the le... -
+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-30553 | |||||||||
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Title | Cryo-EM structure of disome from saccharomyces cerevisiae, the leading ribosome in the rotated state with A/P and P/E tRNAs | |||||||||
Map data | Cryo-EM structure of disome from saccharomyces cerevisiae. leading ribosome in the rotated state with A/P and P/E tRNAs | |||||||||
Sample |
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Biological species | Saccharomyces cerevisiae (brewer's yeast) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 4.59 Å | |||||||||
Authors | Yu L / Taolan Z / Yanming C | |||||||||
Funding support | China, 1 items
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Citation | Journal: To Be Published Title: Cryo-EM structure of disome from saccharomyces cerevisiae, the leading ribosome in the rotated state with A/P and P/E tRNAs Authors: Yu L / Taolan Z / Yanming C | |||||||||
History |
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-Structure visualization
Movie |
Movie viewer |
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Structure viewer | EM map: SurfViewMolmilJmol/JSmol |
Supplemental images |
-Downloads & links
-EMDB archive
Map data | emd_30553.map.gz | 240.1 MB | EMDB map data format | |
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Header (meta data) | emd-30553-v30.xml emd-30553.xml | 7.2 KB 7.2 KB | Display Display | EMDB header |
Images | emd_30553.png | 27.8 KB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-30553 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-30553 | HTTPS FTP |
-Validation report
Summary document | emd_30553_validation.pdf.gz | 332.4 KB | Display | EMDB validaton report |
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Full document | emd_30553_full_validation.pdf.gz | 332 KB | Display | |
Data in XML | emd_30553_validation.xml.gz | 7.4 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-30553 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-30553 | HTTPS FTP |
-Related structure data
Related structure data | C: citing same article (ref.) |
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Similar structure data |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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Related items in Molecule of the Month |
-Map
File | Download / File: emd_30553.map.gz / Format: CCP4 / Size: 325 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Annotation | Cryo-EM structure of disome from saccharomyces cerevisiae. leading ribosome in the rotated state with A/P and P/E tRNAs | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.356 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
-Sample components
-Entire : Cryo-EM structure of disome from saccharomyces cerevisiae, the le...
Entire | Name: Cryo-EM structure of disome from saccharomyces cerevisiae, the leading ribosome in the rotated state with A/P and P/E tRNAs |
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Components |
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-Supramolecule #1: Cryo-EM structure of disome from saccharomyces cerevisiae, the le...
Supramolecule | Name: Cryo-EM structure of disome from saccharomyces cerevisiae, the leading ribosome in the rotated state with A/P and P/E tRNAs type: complex / ID: 1 / Parent: 0 |
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Source (natural) | Organism: Saccharomyces cerevisiae (brewer's yeast) |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 7.5 |
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Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 34.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: SPOT SCAN / Imaging mode: BRIGHT FIELD |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
-Image processing
Startup model | Type of model: EMDB MAP EMDB ID: |
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Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 4.59 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 27599 |
Initial angle assignment | Type: ANGULAR RECONSTITUTION |
Final angle assignment | Type: ANGULAR RECONSTITUTION |