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- EMDB-30553: Cryo-EM structure of disome from saccharomyces cerevisiae, the le... -
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Open data
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Basic information
Entry | Database: EMDB / ID: EMD-30553 | |||||||||
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Title | Cryo-EM structure of disome from saccharomyces cerevisiae, the leading ribosome in the rotated state with A/P and P/E tRNAs | |||||||||
![]() | Cryo-EM structure of disome from saccharomyces cerevisiae. leading ribosome in the rotated state with A/P and P/E tRNAs | |||||||||
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Biological species | ![]() ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 4.59 Å | |||||||||
![]() | Yu L / Taolan Z / Yanming C | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Cryo-EM structure of disome from saccharomyces cerevisiae, the leading ribosome in the rotated state with A/P and P/E tRNAs Authors: Yu L / Taolan Z / Yanming C | |||||||||
History |
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Structure visualization
Movie |
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Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 240.1 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 7.2 KB 7.2 KB | Display Display | ![]() |
Images | ![]() | 27.8 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 332.4 KB | Display | ![]() |
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Full document | ![]() | 332 KB | Display | |
Data in XML | ![]() | 7.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | C: citing same article ( |
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Similar structure data |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
File | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Annotation | Cryo-EM structure of disome from saccharomyces cerevisiae. leading ribosome in the rotated state with A/P and P/E tRNAs | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.356 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
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Sample components
-Entire : Cryo-EM structure of disome from saccharomyces cerevisiae, the le...
Entire | Name: Cryo-EM structure of disome from saccharomyces cerevisiae, the leading ribosome in the rotated state with A/P and P/E tRNAs |
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Components |
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-Supramolecule #1: Cryo-EM structure of disome from saccharomyces cerevisiae, the le...
Supramolecule | Name: Cryo-EM structure of disome from saccharomyces cerevisiae, the leading ribosome in the rotated state with A/P and P/E tRNAs type: complex / ID: 1 / Parent: 0 |
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Source (natural) | Organism: ![]() ![]() |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 7.5 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 34.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: SPOT SCAN / Imaging mode: BRIGHT FIELD |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
Startup model | Type of model: EMDB MAP EMDB ID: |
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Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 4.59 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 27599 |
Initial angle assignment | Type: ANGULAR RECONSTITUTION |
Final angle assignment | Type: ANGULAR RECONSTITUTION |