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- EMDB-3013: Electron cryo-microscopy of Cowpea Mosaic Virus containing RNA-1 ... -
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Open data
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Basic information
Entry | Database: EMDB / ID: EMD-3013 | |||||||||
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Title | Electron cryo-microscopy of Cowpea Mosaic Virus containing RNA-1 (CPMVb) | |||||||||
![]() | Single particle reconstruction of wild type Cowpea mosaic virus containing RNA-1 (CPMVb) | |||||||||
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![]() | CPMV / virus / comoviridae / picornavirales. | |||||||||
Function / homology | ![]() transport of virus in host, cell to cell / host cell plasmodesma / T=3 icosahedral viral capsid / virus-mediated perturbation of host defense response / host cell nucleus / GTP binding / structural molecule activity / DNA binding / RNA binding Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.44 Å | |||||||||
![]() | Hesketh EL / Meshcheriakova Y / Dent KC / Saxena P / Thompson R / Reddy V / Cockburn JJ / Lomonossoff GP / Ranson NA | |||||||||
![]() | ![]() Title: Mechanisms of assembly and genome packaging in an RNA virus revealed by high-resolution cryo-EM. Authors: Emma L Hesketh / Yulia Meshcheriakova / Kyle C Dent / Pooja Saxena / Rebecca F Thompson / Joseph J Cockburn / George P Lomonossoff / Neil A Ranson / ![]() Abstract: Cowpea mosaic virus is a plant-infecting member of the Picornavirales and is of major interest in the development of biotechnology applications. Despite the availability of >100 crystal structures of ...Cowpea mosaic virus is a plant-infecting member of the Picornavirales and is of major interest in the development of biotechnology applications. Despite the availability of >100 crystal structures of Picornavirales capsids, relatively little is known about the mechanisms of capsid assembly and genome encapsidation. Here we have determined cryo-electron microscopy reconstructions for the wild-type virus and an empty virus-like particle, to 3.4 Å and 3.0 Å resolution, respectively, and built de novo atomic models of their capsids. These new structures reveal the C-terminal region of the small coat protein subunit, which is essential for virus assembly and which was missing from previously determined crystal structures, as well as residues that bind to the viral genome. These observations allow us to develop a new model for genome encapsidation and capsid assembly. | |||||||||
History |
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Structure visualization
Movie |
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Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 199.2 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 9.5 KB 9.5 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 14 KB | Display | ![]() |
Images | ![]() | 488.7 KB | ||
Others | ![]() | 165.9 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 347.3 KB | Display | ![]() |
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Full document | ![]() | 346.4 KB | Display | |
Data in XML | ![]() | 13 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 5a32MC ![]() 3014C ![]() 5a33C M: atomic model generated by this map C: citing same article ( |
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Similar structure data |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
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Annotation | Single particle reconstruction of wild type Cowpea mosaic virus containing RNA-1 (CPMVb) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.04 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
-Supplemental map: emd 3013 additional 1.map
File | emd_3013_additional_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
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Sample components
-Entire : Cowpea mosaic virus containing RNA-1
Entire | Name: Cowpea mosaic virus containing RNA-1 |
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Components |
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-Supramolecule #1000: Cowpea mosaic virus containing RNA-1
Supramolecule | Name: Cowpea mosaic virus containing RNA-1 / type: sample / ID: 1000 / Number unique components: 1 |
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Molecular weight | Theoretical: 3.71 MDa |
-Supramolecule #1: Cowpea mosaic virus
Supramolecule | Name: Cowpea mosaic virus / type: virus / ID: 1 / Name.synonym: CPMV / Details: Wild-type virus containing RNA-1 / NCBI-ID: 12264 / Sci species name: Cowpea mosaic virus / Virus type: VIRION / Virus isolate: SPECIES / Virus enveloped: No / Virus empty: No / Syn species name: CPMV |
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Host (natural) | Organism: ![]() ![]() |
Molecular weight | Theoretical: 3.71 MDa |
Virus shell | Shell ID: 1 / Diameter: 280 Å / T number (triangulation number): 3 |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Concentration | 5.8 mg/mL |
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Grid | Details: 200 mesh quantifoil grids with 2 um holes, glow discharged |
Vitrification | Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 94 K / Instrument: FEI VITROBOT MARK IV |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Temperature | Min: 90 K / Max: 90 K |
Date | Nov 1, 2014 |
Image recording | Category: CCD / Film or detector model: FEI FALCON II (4k x 4k) / Number real images: 1754 / Average electron dose: 45 e/Å2 Details: Images are averages of 16 images recorded on a Falcon II direct detector |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Calibrated magnification: 134615 / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 8.0 µm / Nominal defocus min: 0.5 µm / Nominal magnification: 134615 |
Sample stage | Specimen holder: Liquid nitrogen cooled / Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |