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データを開く
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基本情報
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タイトル | Hsp90 provides platform for CRaf dephosphorylation by PP5 | |||||||||||||||
![]() | Composite map used for model fitting. | |||||||||||||||
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機能・相同性 | ![]() regulation of type II interferon-mediated signaling pathway / : / response to arachidonic acid / HSP90-CDC37 chaperone complex / positive regulation of cyclin-dependent protein kinase activity / death-inducing signaling complex assembly / positive regulation of mitophagy in response to mitochondrial depolarization / negative regulation of proteasomal protein catabolic process / Aryl hydrocarbon receptor signalling / peptidyl-serine dephosphorylation ...regulation of type II interferon-mediated signaling pathway / : / response to arachidonic acid / HSP90-CDC37 chaperone complex / positive regulation of cyclin-dependent protein kinase activity / death-inducing signaling complex assembly / positive regulation of mitophagy in response to mitochondrial depolarization / negative regulation of proteasomal protein catabolic process / Aryl hydrocarbon receptor signalling / peptidyl-serine dephosphorylation / peptidyl-threonine dephosphorylation / aryl hydrocarbon receptor complex / dynein axonemal particle / intermediate filament cytoskeleton organization / histone methyltransferase binding / type B pancreatic cell proliferation / regulation of Rho protein signal transduction / SHOC2 M1731 mutant abolishes MRAS complex function / Gain-of-function MRAS complexes activate RAF signaling / positive regulation of protein localization to cell surface / Rap1 signalling / ATP-dependent protein binding / protein kinase regulator activity / regulation of cell motility / response to morphine / protein folding chaperone complex / insulin secretion involved in cellular response to glucose stimulus / negative regulation of protein metabolic process / positive regulation of tau-protein kinase activity / Negative feedback regulation of MAPK pathway / post-transcriptional regulation of gene expression / myosin phosphatase activity / telomerase holoenzyme complex assembly / Respiratory syncytial virus genome replication / regulation of cyclin-dependent protein serine/threonine kinase activity / Uptake and function of diphtheria toxin / GP1b-IX-V activation signalling / IFNG signaling activates MAPKs / protein serine/threonine phosphatase activity / Drug-mediated inhibition of ERBB2 signaling / Resistance of ERBB2 KD mutants to trastuzumab / Resistance of ERBB2 KD mutants to sapitinib / Resistance of ERBB2 KD mutants to tesevatinib / Resistance of ERBB2 KD mutants to neratinib / Resistance of ERBB2 KD mutants to osimertinib / Resistance of ERBB2 KD mutants to afatinib / Resistance of ERBB2 KD mutants to AEE788 / Resistance of ERBB2 KD mutants to lapatinib / Drug resistance in ERBB2 TMD/JMD mutants / protein-serine/threonine phosphatase / TPR domain binding / ERBB2-ERBB3 signaling pathway / positive regulation of transforming growth factor beta receptor signaling pathway / regulation of cell differentiation / Assembly and release of respiratory syncytial virus (RSV) virions / face development / pseudopodium / phosphatase activity / dendritic growth cone / somatic stem cell population maintenance / regulation of type I interferon-mediated signaling pathway / positive regulation of phosphoprotein phosphatase activity / phosphoprotein phosphatase activity / thyroid gland development / neurotrophin TRK receptor signaling pathway / Sema3A PAK dependent Axon repulsion / The NLRP3 inflammasome / regulation of protein ubiquitination / HSF1-dependent transactivation / response to unfolded protein / negative regulation of proteasomal ubiquitin-dependent protein catabolic process / telomere maintenance via telomerase / extrinsic apoptotic signaling pathway via death domain receptors / chaperone-mediated protein complex assembly / MAP kinase kinase kinase activity / HSF1 activation / Attenuation phase / protein targeting / cellular response to interleukin-4 / negative regulation of protein-containing complex assembly / RHOBTB2 GTPase cycle / Purinergic signaling in leishmaniasis infection / Schwann cell development / axonal growth cone / type II interferon-mediated signaling pathway / DNA polymerase binding / supramolecular fiber organization / negative regulation of extrinsic apoptotic signaling pathway via death domain receptors / Signaling by ERBB2 / : / heat shock protein binding / HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand / activation of adenylate cyclase activity / response to muscle stretch / nitric-oxide synthase regulator activity / myelination / CD209 (DC-SIGN) signaling / Constitutive Signaling by Overexpressed ERBB2 / protein dephosphorylation / ESR-mediated signaling 類似検索 - 分子機能 | |||||||||||||||
生物種 | ![]() | |||||||||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.8 Å | |||||||||||||||
![]() | Jaime-Garza M / Nowotny CA / Coutandin D / Wang F / Tabios M / Agard DA | |||||||||||||||
資金援助 | ![]()
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![]() | ![]() タイトル: Hsp90 provides a platform for kinase dephosphorylation by PP5. 著者: Maru Jaime-Garza / Carlos A Nowotny / Daniel Coutandin / Feng Wang / Mariano Tabios / David A Agard / ![]() 要旨: The Hsp90 molecular chaperone collaborates with the phosphorylated Cdc37 cochaperone for the folding and activation of its many client kinases. As with many kinases, the Hsp90 client kinase CRaf is ...The Hsp90 molecular chaperone collaborates with the phosphorylated Cdc37 cochaperone for the folding and activation of its many client kinases. As with many kinases, the Hsp90 client kinase CRaf is activated by phosphorylation at specific regulatory sites. The cochaperone phosphatase PP5 dephosphorylates CRaf and Cdc37 in an Hsp90-dependent manner. Although dephosphorylating Cdc37 has been proposed as a mechanism for releasing Hsp90-bound kinases, here we show that Hsp90 bound kinases sterically inhibit Cdc37 dephosphorylation indicating kinase release must occur before Cdc37 dephosphorylation. Our cryo-EM structure of PP5 in complex with Hsp90:Cdc37:CRaf reveals how Hsp90 both activates PP5 and scaffolds its association with the bound CRaf to dephosphorylate phosphorylation sites neighboring the kinase domain. Thus, we directly show how Hsp90's role in maintaining protein homeostasis goes beyond folding and activation to include post translationally modifying its client kinases. | |||||||||||||||
履歴 |
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構造の表示
添付画像 |
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ダウンロードとリンク
-EMDBアーカイブ
マップデータ | ![]() | 108.4 MB | ![]() | |
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ヘッダ (付随情報) | ![]() ![]() | 32.3 KB 32.3 KB | 表示 表示 | ![]() |
FSC (解像度算出) | ![]() | 11.5 KB | 表示 | ![]() |
画像 | ![]() | 89.6 KB | ||
その他 | ![]() ![]() ![]() | 116.4 MB 75.1 MB 75 MB | ||
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-検証レポート
文書・要旨 | ![]() | 780.2 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 779.8 KB | 表示 | |
XML形式データ | ![]() | 18.4 KB | 表示 | |
CIF形式データ | ![]() | 24.2 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
関連構造データ | ![]() 8gftMC ![]() 8gaeC C: 同じ文献を引用 ( M: このマップから作成された原子モデル |
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類似構造データ | 類似検索 - 機能・相同性 ![]() |
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リンク
EMDBのページ | ![]() ![]() |
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「今月の分子」の関連する項目 |
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マップ
ファイル | ![]() | ||||||||||||||||||||
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注釈 | Composite map used for model fitting. | ||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 0.835 Å | ||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||
詳細 | EMDB XML:
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-添付データ
-追加マップ: Postprocessed composite map which gave FSC curve file,...
ファイル | emd_29984_additional_1.map | ||||||||||||
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注釈 | Postprocessed composite map which gave FSC curve file, map resolution, and was used for model fitting. | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
-ハーフマップ: Composite half map.
ファイル | emd_29984_half_map_1.map | ||||||||||||
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注釈 | Composite half map. | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
-ハーフマップ: Composite half map.
ファイル | emd_29984_half_map_2.map | ||||||||||||
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注釈 | Composite half map. | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
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試料の構成要素
+全体 : Hsp90:Cdc37:CRaf:PP5 complex
+超分子 #1: Hsp90:Cdc37:CRaf:PP5 complex
+超分子 #2: Protein Phosphatase 5 (H304A)
+分子 #1: Heat shock protein HSP 90-beta
+分子 #2: Hsp90 co-chaperone Cdc37, N-terminally processed
+分子 #3: RAF proto-oncogene serine/threonine-protein kinase
+分子 #4: Serine/threonine-protein phosphatase 5
+分子 #5: ADENOSINE-5'-DIPHOSPHATE
+分子 #6: MAGNESIUM ION
+分子 #7: POTASSIUM ION
+分子 #8: ADENOSINE-5'-TRIPHOSPHATE
+分子 #9: MANGANESE (II) ION
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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![]() | 単粒子再構成法 |
試料の集合状態 | particle |
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試料調製
濃度 | 0.09 mg/mL | ||||||||||||||||||
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緩衝液 | pH: 7.5 構成要素:
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グリッド | モデル: Quantifoil R1.2/1.3 / 材質: GOLD / メッシュ: 300 / 支持フィルム - 材質: GRAPHENE OXIDE / 支持フィルム - トポロジー: CONTINUOUS / 支持フィルム - Film thickness: 1.0 nm | ||||||||||||||||||
凍結 | 凍結剤: ETHANE / チャンバー内湿度: 100 % / チャンバー内温度: 283.15 K 詳細: 3uL OF SAMPLE, 10C, 100% HUMIDITY, 30S WAIT TIME, 3S BLOT TIME, -2 BLOT FORCE. |
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電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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ソフトウェア | 名称: SerialEM (ver. 3.8-beta) |
撮影 | フィルム・検出器のモデル: GATAN K3 BIOQUANTUM (6k x 4k) 実像数: 4160 / 平均電子線量: 69.0 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: ![]() |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / 最大 デフォーカス(公称値): 1.8 µm / 最小 デフォーカス(公称値): 0.8 µm / 倍率(公称値): 105000 |
試料ステージ | 試料ホルダーモデル: FEI TITAN KRIOS AUTOGRID HOLDER ホルダー冷却材: NITROGEN |
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |
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画像解析
-原子モデル構築 1
初期モデル | (Chain: PDB, experimental model, PDB, experimental model, PDB, experimental model) |
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ソフトウェア | 名称: ISOLDE (ver. 1.0b3) |
詳細 | This model was built using rigid body docking in Chimera and ChimeraX for all main chains, and RosettaCM to add remaining fragments not included in previous models. Refinement was done using iterative Phenix and RosettaRelax, and finalized with ISOLDE. |
精密化 | プロトコル: RIGID BODY FIT |
得られたモデル | ![]() PDB-8gft: |