ジャーナル: Nat Commun / 年: 2023 タイトル: Native doublet microtubules from Tetrahymena thermophila reveal the importance of outer junction proteins. 著者: Shintaroh Kubo / Corbin S Black / Ewa Joachimiak / Shun Kai Yang / Thibault Legal / Katya Peri / Ahmad Abdelzaher Zaki Khalifa / Avrin Ghanaeian / Caitlyn L McCafferty / Melissa Valente- ...著者: Shintaroh Kubo / Corbin S Black / Ewa Joachimiak / Shun Kai Yang / Thibault Legal / Katya Peri / Ahmad Abdelzaher Zaki Khalifa / Avrin Ghanaeian / Caitlyn L McCafferty / Melissa Valente-Paterno / Chelsea De Bellis / Phuong M Huynh / Zhe Fan / Edward M Marcotte / Dorota Wloga / Khanh Huy Bui / 要旨: Cilia are ubiquitous eukaryotic organelles responsible for cellular motility and sensory functions. The ciliary axoneme is a microtubule-based cytoskeleton consisting of two central singlets and nine ...Cilia are ubiquitous eukaryotic organelles responsible for cellular motility and sensory functions. The ciliary axoneme is a microtubule-based cytoskeleton consisting of two central singlets and nine outer doublet microtubules. Cryo-electron microscopy-based studies have revealed a complex network inside the lumen of both tubules composed of microtubule-inner proteins (MIPs). However, the functions of most MIPs remain unknown. Here, we present single-particle cryo-EM-based analyses of the Tetrahymena thermophila native doublet microtubule and identify 42 MIPs. These data shed light on the evolutionarily conserved and diversified roles of MIPs. In addition, we identified MIPs potentially responsible for the assembly and stability of the doublet outer junction. Knockout of the evolutionarily conserved outer junction component CFAP77 moderately diminishes Tetrahymena swimming speed and beat frequency, indicating the important role of CFAP77 and outer junction stability in cilia beating generation and/or regulation.
全体 : 96-nm repeat unit of microtubule doublet from Tetrahymena thermophila
全体
名称: 96-nm repeat unit of microtubule doublet from Tetrahymena thermophila
要素
細胞器官・細胞要素: 96-nm repeat unit of microtubule doublet from Tetrahymena thermophila
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超分子 #1: 96-nm repeat unit of microtubule doublet from Tetrahymena thermophila
超分子
名称: 96-nm repeat unit of microtubule doublet from Tetrahymena thermophila タイプ: organelle_or_cellular_component / ID: 1 / 親要素: 0 詳細: Single Particle Analysis of the 96-nm repeating unit of the axoneme from intact cilia purified from Tetrahymena strains CU428 and K40R