EMDB-29685: 48-nm doublet microtubule from Tetrahymena thermophila strain CU428

Basic information

Entry

Database: EMDB / ID: EMD-29685

• Title: 48-nm doublet microtubule from Tetrahymena thermophila strain CU428
• Map data: composite map of 48-nm doublet from Tetrahymena strain CU428

Sample

• Organelle or cellular component: 48-nm repeat unit of microtubule doublet from Tetrahymena thermophila strain CU428
  • Protein or peptide: x 46 types
• Ligand: x 3 types

• Keywords: Cilia / Axoneme / Doublet Microtubule / Microtubule Inner Protein / STRUCTURAL PROTEIN

Function / homology

Function:

cilium-dependent cell motility / axoneme assembly / axonemal microtubule / nucleoside-diphosphate kinase / UTP biosynthetic process / CTP biosynthetic process / motile cilium / nucleoside diphosphate kinase activity / GTP biosynthetic process / mitotic cytokinesis / axoneme / cilium assembly / alpha-tubulin binding / microtubule-based process / Hsp70 protein binding / mitotic spindle organization / meiotic cell cycle / cell projection / Hsp90 protein binding / structural constituent of cytoskeleton / mitotic spindle / Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement / microtubule / cytoskeleton / hydrolase activity / calmodulin binding / cilium / GTPase activity / calcium ion binding / GTP binding / zinc ion binding / ATP binding / metal ion binding / nucleus / cytoplasm

Domain/homology:

CCDC81, HU domain 1 / CCDC81, HU domain 2 / CCDC81 eukaryotic HU domain 1 / CCDC81 eukaryotic HU domain 2 / Sperm-tail PG-rich repeat / Sperm-tail PG-rich repeat / : / : / Uncharacterised protein FAM166/UPF0605 / Cilia- and flagella-associated protein 77 / Ciliary microtubule inner protein 2B-like / Cilia- and flagella-associated protein 77 / Nucleoside diphosphate kinase 7 / Piercer of microtubule wall 1/2 / Piercer of microtubule wall 1/2 / Cilia- and flagella-associated protein 161-like domain / Cilia- and flagella-associated protein 141 / Cilia- and flagella-associated protein 141 / : / : / Meiosis-specific nuclear structural protein 1 / Cilia- and flagella-associated protein 45 / NDPK7, second NDPk domain / Cilia- and flagella- associated protein 210 / CFAP53/TCHP / Trichohyalin-plectin-homology domain / Trichohyalin-plectin-homology domain / RIB43A / RIB43A / DM10 domain / EF-hand domain-containing protein EFHC1/EFHC2/EFHB / DM10 domain / DM10 domain profile. / Domains in hypothetical proteins in Drosophila, C. elegans and mammals. Occurs singly in some nucleoside diphosphate kinases. / Enkurin domain / : / Calmodulin-binding / Enkurin domain profile. / : / CFA20 domain / Cilia- and flagella-associated protein 20/CFAP20DC / CFA20 domain / Parkin co-regulated protein / Parkin co-regulated protein / Dienelactone hydrolase / Dienelactone hydrolase family / Nucleoside diphosphate kinase, active site / Nucleoside diphosphate kinase (NDPK) active site signature. / Nucleoside diphosphate kinase / Nucleoside diphosphate kinase-like domain / Nucleoside diphosphate kinase / NDK / Nucleoside diphosphate kinase-like domain superfamily / EF-hand domain pair / Mir domain superfamily / Tubulin-beta mRNA autoregulation signal. / Alpha tubulin / Beta tubulin, autoregulation binding site / Beta tubulin / Tubulin / Tubulin, C-terminal / Tubulin C-terminal domain / Tubulin, conserved site / Tubulin subunits alpha, beta, and gamma signature. / Tubulin/FtsZ family, C-terminal domain / Tubulin/FtsZ-like, C-terminal domain / Tubulin/FtsZ, C-terminal / Tubulin/FtsZ, 2-layer sandwich domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ, GTPase domain / Tubulin/FtsZ, GTPase domain superfamily / EF-hand domain pair / EF-hand, calcium binding motif / EF-Hand 1, calcium-binding site / EF-hand calcium-binding domain. / EF-hand calcium-binding domain profile. / EF-hand domain / EF-hand domain pair / Alpha/Beta hydrolase fold / Armadillo-type fold / WD40 repeat, conserved site / Trp-Asp (WD) repeats signature. / WD domain, G-beta repeat / Trp-Asp (WD) repeats profile. / Trp-Asp (WD) repeats circular profile. / WD40 repeats / WD40 repeat / WD40-repeat-containing domain superfamily / WD40/YVTN repeat-like-containing domain superfamily

Component:

Flagellar FliJ protein / Uncharacterized protein / RIB43A protein / EF hand protein / Uncharacterized protein / Meiosis-specific nuclear structural protein 1 / Flagellar microtugule protofilament ribbon protein / DNA polymerase delta C4-type zinc-finger protein / Sperm-tail PG-rich repeat protein / Parkin co-regulated protein / Ptype ATPase, putative / Uncharacterized protein / EF-hand protein / EF-hand pair protein / EF-hand pair protein / Uncharacterized protein / Parkin co-regulated protein / Uncharacterized protein / Tubulin alpha chain / Tubulin beta chain / Uncharacterized protein / Uncharacterized protein / Transcription factor iib protein, putative / EF hand protein, putative / Sperm-tail PG-rich repeat protein / Cilia- and flagella-associated protein 52 / Uncharacterized protein / Uncharacterized protein / Dienelactone hydrolase family protein / Ciliary microtubule inner protein 2A-C-like domain-containing protein / Uncharacterized protein / Uncharacterized protein / Ciliary microtubule inner protein 2A-C-like domain-containing protein / Trichohyalin-plectin-homology domain-containing protein / STOP protein / EF hand protein / Cilia- and flagella-associated protein 53 / Protofilament ribbon protein / Uncharacterized protein / Sperm-tail PG-rich repeat protein / Cilia- and flagella-associated protein 45 / Nucleoside diphosphate kinase

• Biological species: Tetrahymena thermophila (eukaryote)
• Method: single particle reconstruction / cryo EM / Resolution: 4.1 Å
• Authors: Black CS / Kubo S / Yang SK / Bui KH

Funding support

Canada, 1 items

Organization	Grant number	Country
Canadian Institutes of Health Research (CIHR)		Canada

• Citation: Journal: Nat Commun / Year: 2023; Title: Native doublet microtubules from Tetrahymena thermophila reveal the importance of outer junction proteins.; Authors: Shintaroh Kubo / Corbin S Black / Ewa Joachimiak / Shun Kai Yang / Thibault Legal / Katya Peri / Ahmad Abdelzaher Zaki Khalifa / Avrin Ghanaeian / Caitlyn L McCafferty / Melissa Valente-Paterno / Chelsea De Bellis / Phuong M Huynh / Zhe Fan / Edward M Marcotte / Dorota Wloga / Khanh Huy Bui / ; Abstract: Cilia are ubiquitous eukaryotic organelles responsible for cellular motility and sensory functions. The ciliary axoneme is a microtubule-based cytoskeleton consisting of two central singlets and nine outer doublet microtubules. Cryo-electron microscopy-based studies have revealed a complex network inside the lumen of both tubules composed of microtubule-inner proteins (MIPs). However, the functions of most MIPs remain unknown. Here, we present single-particle cryo-EM-based analyses of the Tetrahymena thermophila native doublet microtubule and identify 42 MIPs. These data shed light on the evolutionarily conserved and diversified roles of MIPs. In addition, we identified MIPs potentially responsible for the assembly and stability of the doublet outer junction. Knockout of the evolutionarily conserved outer junction component CFAP77 moderately diminishes Tetrahymena swimming speed and beat frequency, indicating the important role of CFAP77 and outer junction stability in cilia beating generation and/or regulation.

History

Deposition	Feb 6, 2023	-
Header (metadata) release	Jun 14, 2023	-
Map release	Jun 14, 2023	-
Update	Dec 25, 2024	-
Current status	Dec 25, 2024	Processing site: RCSB / Status: Released

Map

• File: Download / File: emd_29685.map.gz / Format: CCP4 / Size: 512 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Annotation: composite map of 48-nm doublet from Tetrahymena strain CU428
• Voxel size: X=Y=Z: 1.37 Å

Density

Contour Level	By AUTHOR: 0.15
Minimum - Maximum	-0.004287836 - 2.3559375
Average (Standard dev.)	0.0109767355 (±0.07871197)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 512 512 512 Spacing 512 512 512
Cell	A=B=C: 701.44 Å α=β=γ: 90.0 °

Supplemental data

Mask #1

• File: emd_29685_msk_1.map

Half map: half map 2 of 48-nm doublet from Tetrahymena strain CU428

• File: emd_29685_half_map_1.map
• Annotation: half map 2 of 48-nm doublet from Tetrahymena strain CU428

Half map: half map 1 of 48-nm doublet from Tetrahymena strain CU428

• File: emd_29685_half_map_2.map
• Annotation: half map 1 of 48-nm doublet from Tetrahymena strain CU428

Sample components

Entire : 48-nm repeat unit of microtubule doublet from Tetrahymena thermop...

• Entire: Name: 48-nm repeat unit of microtubule doublet from Tetrahymena thermophila strain CU428

Components

• Organelle or cellular component: 48-nm repeat unit of microtubule doublet from Tetrahymena thermophila strain CU428
  • Protein or peptide: Tubulin beta chain
  • Protein or peptide: Tubulin alpha chain
  • Protein or peptide: OJ3
  • Protein or peptide: CFAP115
  • Protein or peptide: RIB57
  • Protein or peptide: RIB35
  • Protein or peptide: RIB43A protein
  • Protein or peptide: Protofilament ribbon protein
  • Protein or peptide: CFAP210
  • Protein or peptide: CFAP182B
  • Protein or peptide: CFAP182A
  • Protein or peptide: CFAP161A
  • Protein or peptide: CFAP143
  • Protein or peptide: Flagellar FliJ protein
  • Protein or peptide: CFAP129
  • Protein or peptide: CFAP127
  • Protein or peptide: DNA polymerase delta C4-type zinc-finger protein
  • Protein or peptide: Nucleoside diphosphate kinase
  • Protein or peptide: CFAP53
  • Protein or peptide: CFAP52A
  • Protein or peptide: Cilia- and flagella-associated protein 45
  • Protein or peptide: CFAP21A
  • Protein or peptide: CFAP20
  • Protein or peptide: RIB72B
  • Protein or peptide: Flagellar microtubule protofilament ribbon protein
  • Protein or peptide: CFAM166A
  • Protein or peptide: CFAP107
  • Protein or peptide: CFAM166B
  • Protein or peptide: RIB26
  • Protein or peptide: Nebulin
  • Protein or peptide: SB1
  • Protein or peptide: STPG1A
  • Protein or peptide: RIB38
  • Protein or peptide: RIB27B
  • Protein or peptide: CFAM166C
  • Protein or peptide: Parkin co-regulated protein PACRGB
  • Protein or peptide: Parkin co-regulated protein PACRGA
  • Protein or peptide: OJ2
  • Protein or peptide: IJ34
  • Protein or peptide: STPG2
  • Protein or peptide: RIB27A
  • Protein or peptide: RIB22
  • Protein or peptide: CFAP77A
  • Protein or peptide: B5B6_fMIP
  • Protein or peptide: CFAP112A
  • Protein or peptide: B2B3_fMIP
• Ligand: GUANOSINE-5'-TRIPHOSPHATE
• Ligand: MAGNESIUM ION
• Ligand: GUANOSINE-5'-DIPHOSPHATE

Supramolecule #1: 48-nm repeat unit of microtubule doublet from Tetrahymena thermop...

• Supramolecule: Name: 48-nm repeat unit of microtubule doublet from Tetrahymena thermophila strain CU428; type: organelle_or_cellular_component / ID: 1 / Parent: 0 ; Macromolecule list: #46, #45, #38, #21, #16, #27, #15, #26, #24, #28, #17, #9, #18, #29, #33, #8, #14, #22, #20, #13, #23, #19, #10, #25, #34, #4, #7, #3, #32, #41, #39-#40, #2, #30, #6, #35, #11, #36, #12, #31, #1, #5, #37, #42-#44; Details: Single Particle Analysis of the 48-nm repeating unit of the axoneme from intact cilia purified from Tetrahymena strain CU428
• Source (natural): Organism: Tetrahymena thermophila (eukaryote) / Strain: CU428 / Organelle: Cilia
• Molecular weight: Theoretical: 7.6 MDa

Macromolecule #1: RIB27A

• Macromolecule: Name: RIB27A / type: protein_or_peptide / ID: 1 / Number of copies: 4 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila (eukaryote) / Strain: CU428
• Molecular weight: Theoretical: 27.605641 KDa

Sequence

String:

MSQYAYDFNP AKTQSLQSRP IEHNKFSAWT GDQLYRTSYG THWTSKPQEP KTHVPPGYAG YIPGLKPNNH YGASYGEIAK NCLSNPKVA QNPFKLASTG FNYQRHDFRD PSLTATTHKF GAQTLLKNHP SIDQKSNQWQ SQTHDSFRNP LHKPNPTYRE T DKDLQTQK YFTKTSGFQQ NHTTFDRTGW VPEKVLHADR TTSEYRIHFN KQVPFHRDTV LFKERRLPPK EYNYKYMG

UniProtKB: Uncharacterized protein

Macromolecule #2: RIB38

• Macromolecule: Name: RIB38 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila (eukaryote) / Strain: CU428
• Molecular weight: Theoretical: 38.205637 KDa

Sequence

String:

MAQACDKYTT ALMMGNWYEE RLAPNQPFRE NLQTKSVREE EAAISFASDT NKLIPLSRVN RNLPWDTKGV IADDGFKEFR SVNRTSYDP INLNNYYNLG DCRQLYKHYT AEKKYPENNP TQIQTLKANQ YSLLNQTNSQ HIHKERQVPN NVSDFGSTFK K FPETHEKF FGMTTYQQSF SRPLKETAHQ IIAQSEKKIN HFAGTNERPV HQQSTKMTSV LTAEKYKNEQ EPKDNTSIQR SW LPYKDKA LQVAENNLIK NQTLNQTKGF TPFNPLITYK NNMSQVQQHD IATSLPIGSG EHSDQKRTNQ PGEFRHVRTD VTL IRNKPV TKR

UniProtKB: STOP protein

Macromolecule #3: CFAM166B

• Macromolecule: Name: CFAM166B / type: protein_or_peptide / ID: 3 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila (eukaryote) / Strain: CU428
• Molecular weight: Theoretical: 17.705826 KDa

Sequence

String:

MYLQSKTTNN IPGYTGHIPQ EELHEDILQI NQAKSHIPGY AGYIPGIRSE NMYGKTYGKI TYMSVTNQHH KGPDLPPDLR YTSTVKGEF TDQKGVKKQE LLRQAVIEEA KITSSGYGSS PLRTKYIDPS EYKEKEIQPA PECNLTYQEA CKYAYSN

UniProtKB: Ciliary microtubule inner protein 2A-C-like domain-containing protein

Macromolecule #4: CFAM166A

• Macromolecule: Name: CFAM166A / type: protein_or_peptide / ID: 4 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila (eukaryote) / Strain: CU428
• Molecular weight: Theoretical: 25.750811 KDa

Sequence

String:

MFEGTRLSYV VPGYTGHIPK VIRDDPIPIQ KQEPKYQIPG YDGYIPSIKS ENIFGKTYGK ITYTISKGDQ HQGQDVNAQQ RYASVAEET YINQNQVLQR TAAEIVGVPP KQLQYTLTDK MKDSNFWQTQ LQHTKKLQQE ESQNVRPQQF EESLDKFYGD D KVDPIRIG TPLPGYTGTN KRIVAANIFG QTFANARKTA AADQHNIDNE KMHNFKLQAQ NIPNIRR

UniProtKB: Ciliary microtubule inner protein 2A-C-like domain-containing protein

Macromolecule #5: RIB22

• Macromolecule: Name: RIB22 / type: protein_or_peptide / ID: 5 / Number of copies: 4 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila (eukaryote) / Strain: CU428
• Molecular weight: Theoretical: 22.401135 KDa

Sequence

String:

MNSDLQNLIN RVRSALLSRN QNTIRGLARA FRQLDSYDRN NKVDRDEFFI VLKENGVSLT KQEQFVLHQC FDRNNEGTVD FDEFLCVIR GELNEQRKAI VLKAFQKFDP HNTGFVEIRD LRGVYNCDNH PKIKTGQMTE EQVFVEFLQN FCDNSKDGKI Q LKEWIDYY SAVSVSIQND DHFEQVVKIA WGV

UniProtKB: EF hand protein

Macromolecule #6: CFAM166C

• Macromolecule: Name: CFAM166C / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila (eukaryote) / Strain: CU428
• Molecular weight: Theoretical: 25.069318 KDa

Sequence

String:

MNTTQMIPTY QDVKTGTTWL GGPTHVLQEQ HIPGYKGHVK GVQAESLHGK TFARITAECL NNRYKSGFIV DDEERFKTNY QLEYIRPNL RNNPTFTNNA SNVLNNRQRD DEVERLELIK AQLNSQPKTI EDVPPIDRVP VIGYQGFRPV YRHPLKQVKG P TEDEIDFQ ARTILELTKG QKEAPKEIPI VGYTGFQKGI KAENMFGKTF KDLSNASNRI F

UniProtKB: Uncharacterized protein

Macromolecule #7: CFAP107

• Macromolecule: Name: CFAP107 / type: protein_or_peptide / ID: 7 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila (eukaryote) / Strain: CU428
• Molecular weight: Theoretical: 21.298023 KDa

Sequence

String:

MNAQTIQDNV NKYRFGVLIG NFAEEKFGMD MAQRQIDERL PNSTMKDSYG LKNSALNCEP SKLTPIDKEF NQHVIFNTQG VQQHILFGH GVKQTDYNKR EYGTSYDLSF NQKIKPQTQI YSKYTPDALS ASRTFHKDTI FEKDYQKHIP ELGSKPTVPK D KARPYNEF TKTYDSTHMK IPLRK

UniProtKB: Uncharacterized protein

Macromolecule #8: CFAP127

• Macromolecule: Name: CFAP127 / type: protein_or_peptide / ID: 8 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila (eukaryote) / Strain: CU428
• Molecular weight: Theoretical: 55.558031 KDa

Sequence

String:

MYPKQTQTSY KPQQHPAVTQ RQFIANDTYF NQQHYQRHEI EDALARELQR RKLDDEAKQR EIQKIYEESE ELKLLKEKIR MAQVNKERT RQIAENQLRK FQTKKVEAEL EENILRKCEE EKRYEAEKER ILNEQRMQGR FVLQQQMLEK RQAAEEAHQQ Y LLEKDQVN AIVQNIINED KMAYESDKLK KKRNYQDMVE ALQEKAERKA QEKINEQLEN AKYKKFFEQK EQMQQEIKKK RE ELEAQKA IIFERLKAEE ERRRKEKELI EDLRMELYKE EFEAQERRKD REEREKRERT KQELQEAERQ HKYDKERQLA EEK RMEQQF REKMMEKYAQ DEKLEQLSAQ RRRMKEIDHK KEVERLWQEK LEMYREERER ELEEARKNEE LKSYKDELIR LEKE RLLRE YLDELAGFMP KGLLQTKEDT KFLKNQNPYA QRTGFASNFK IM

UniProtKB: Meiosis-specific nuclear structural protein 1

Macromolecule #9: CFAP161A

• Macromolecule: Name: CFAP161A / type: protein_or_peptide / ID: 9 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila (eukaryote) / Strain: CU428
• Molecular weight: Theoretical: 57.489621 KDa

Sequence

String:

MHNYNVDRKY QYNCRIGNWS EEWELDEYKM KEYLKNKEKQ KLVSTDKEDR LKRSLGGVNL SYANEGLVKF GYTVMIESDF MNAVVASNI WDKITGTEEA YGVTVTSQKE PIVRTAFQLL RYEQDQYQDD VIHYGQNFKI AVISRLSEKP LYLTSSHITP Q KCAKFSRK QEVSISTNEG INSAWCFEYM DPKLRYEMKG QPVRSGEPVL IKHIPTCQWL ATDNVQYNNE FGQEFEVFCN SY QCHNKTQ NLIAEKVGRK TIETPMRNQT NQNTFRIVTA LYPSQETQQN FEASYSQTLP RRRVEINKAD LLNKLRRALI NKN GSNGLL FFKNELLKQD KDNSGYLPQQ QFKQTVQCHG INFSDLDIKE VIQSFQELDN KINYSELLIQ LKGTLSEKRR NLIN LAYDV LAEKLGQNVT LSGIFSAFNK IRHPDVVKKY GTDKEIFNNF VLSWGNLNPH IAISREQFES YYHDFSACVD KEDYF EVVL KNSWDIPN

UniProtKB: EF hand protein, putative

Macromolecule #10: CFAP20

• Macromolecule: Name: CFAP20 / type: protein_or_peptide / ID: 10 / Number of copies: 7 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila (eukaryote) / Strain: CU428
• Molecular weight: Theoretical: 22.976535 KDa

Sequence

String:

MFKNTFQSGF LSILYSIGSK PLQIWDKSIR NGHIKRITDQ DILSSVLEIM GTNVSTNYIT APADPKETLG IKLPFLVMII KNLKKYYTF EVQVLDDKNV RRRFRASNYQ STTRVKPFIC TMPMRLDEGW NQIQFNLSDF TRRAYATNYI ETLRVQIHAN C RIRRIYFS DRLYSEEELP PEFKLFLPIQ GQNKTNV

UniProtKB: Transcription factor iib protein, putative

Macromolecule #11: Parkin co-regulated protein PACRGA

• Macromolecule: Name: Parkin co-regulated protein PACRGA / type: protein_or_peptide / ID: 11 / Number of copies: 4 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila (eukaryote) / Strain: CU428
• Molecular weight: Theoretical: 36.304816 KDa

Sequence

String:

MLPKIPQQGN TVGSISKREN VASQLIVEDH LKNILAKSTN SNYKPWKIPQ APKNPHSPFG DFPKEYLPKS KLKSEQHAPV FEDSQAATV VNKFQGLRQG TGGKTSTQLP VKQPFQAPNP ICGAFKKRTI PVSEFRRYYD RGDLPIKVDH QGSVNKIIWV I QPDQLDYH HYLPIFFDGL REKLDPYRFL AILGTYDLLE KGSNKILPVI PQLIIPVKTA LNTRDNEIIG IMLKILQRLV VS GDMIGEA LVPYYRQILP IFNLYKNCNK NVGDQIDYGQ RKKLTLGDLI QETLELFEQT GGEDAFINIK YMIPTYESCV HN

UniProtKB: Parkin co-regulated protein

Macromolecule #12: IJ34

• Macromolecule: Name: IJ34 / type: protein_or_peptide / ID: 12 / Number of copies: 4 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila (eukaryote) / Strain: CU428
• Molecular weight: Theoretical: 34.434062 KDa

Sequence

String:

MNLDSIFEWI LKQPINYPGK HQDSQPRTNP AYKPLEAKAQ FHIIFKATGE YIREKMLDGK GVNMRDFGAF TFEIFSDTVK PAQHSNFDI AKDLDEQRAD RKHVHRIRPC FVPDKKFRYS LARYAGKEEI SAPKSQHSIY QKGFGMMFCN AGPIAASCYL G KDVVSSAH NAFIQAVSDL TTLGHGLNID FGFVKVYVQD RDLRYSYNQN FANTLNNKNF EYRLRKSDTP TSEHWNTTYE SK WAKSSLN SLLKRPNSNQ VRNYYEKTLA LKIMSLDLST AEQTNYSRIK PKPQQLPPLK K

UniProtKB: Uncharacterized protein

Macromolecule #13: CFAP52A

• Macromolecule: Name: CFAP52A / type: protein_or_peptide / ID: 13 / Number of copies: 4 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila (eukaryote) / Strain: CU428
• Molecular weight: Theoretical: 73.429117 KDa

Sequence

String:

MEKELDIQAV IGFTGKVNEG LILHPDNEHL IYPLGSTIVV RHIISRSQTF LRGHDNQISI ITVSKTGKYI ASGQKTYMGF QADIIIWDF ETRSLMHRLK LHKVLIQSLS FCCNEQYLAS LGGQDDKNMM IVWDVEQGKA LYGAPNRDVV NQIKFFNNSD D KIIAVLNN GVQILTIDKA NKKVKSLDVN FGNIKRQYTC VAIDPSDTYC YCGTKTGDVF EINIERAIFK RLGPVKKLFS LG IGTLGLL PNGDIIVGAG DGTVAKLSIQ NMQVLSQSEV LGAVTSISFT GDYTHFFCGT SQSNIYWIDT EKLNPELRNT CHY ERINDI AFPYNYSDVF ATCSTTDIRV WNARNRQELL RIQVPNLECY CVTFMNDGKS IISGWNDGKI RAFLPQSGKL LYVI SDAHI HGVTALSTTS DCQRIVSGGS EGEVRVWVIN KQTQVMKASM KEHRGRVWSI QVKKNNDQAV SASADGSCII WDLKT FTRL MCLFESTLFK QVLYHPEESQ LLTTGSDRKI TYWEIYDGQA IRMLDGSEEG EVNALSITKE GEHFVSGGED KLVKLW GYD EGIKYYSGTG HSGAITRLQI SPDQRTVVSV GAEGAIFIWK MPEEVVHARA DNELPTISKE KHNNTQNQGD NKSSHSQ QH SQVSGASKGS QKRY

UniProtKB: Cilia- and flagella-associated protein 52

Macromolecule #14: DNA polymerase delta C4-type zinc-finger protein

• Macromolecule: Name: DNA polymerase delta C4-type zinc-finger protein / type: protein_or_peptide / ID: 14 / Number of copies: 4 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila (eukaryote) / Strain: CU428
• Molecular weight: Theoretical: 31.493797 KDa

Sequence

String:

MEEESIYNLI PKEYVPPPKE RRYRSQYPND LPPTGSTFIN HTTSRPGVAN LAGDFELSKG PHSHKDFCST FGRVKGSIKP STSDFRKKN TGTMGSNKLP IIKPFKYADH ESRRPNVPKI EEKPLMNQQS HKNYIVTNAV ENILSAPKAM KEPENYLTKR D YGKVPNYL VRNKEHLQTE YKMIQNLHLS EAEEQQRQRF LMSQEEVRQL RDGLKAKWEA VNKEYQTITH INKIDTVGLK RK KEECERE LTQLEKDIEK LNKQYIFVDT NM

UniProtKB: DNA polymerase delta C4-type zinc-finger protein

Macromolecule #15: RIB43A protein

• Macromolecule: Name: RIB43A protein / type: protein_or_peptide / ID: 15 / Number of copies: 5 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila (eukaryote) / Strain: CU428
• Molecular weight: Theoretical: 17.288318 KDa

Sequence

String:

MKRVKESYFR EHSDWSDING CSGAKEFEGE DLAYDARIKY QKETQKQWIE QQIREKKMRE EAERNEERAY ATQTLELNRM RGMLEDDFN RKKASIRQAV KEENQQLDKQ KRDLEKQSNN EKLNYERTEI DMVKTRGQKR PFP

UniProtKB: RIB43A protein

Macromolecule #16: RIB57

• Macromolecule: Name: RIB57 / type: protein_or_peptide / ID: 16 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila (eukaryote) / Strain: CU428
• Molecular weight: Theoretical: 57.580914 KDa

Sequence

String:

MDWINRLENI QNKIKFQLHA KKIDDLESLY RLMAEFDLDN SGRLEKDEFL KLLSKLGVFL TTQELRAVYD TYDTNRDEQI SYAEFVQMI RTTISEKRLA VVKHAFQFLD RRGNGKLAIQ DLVQQYRSEA HPRVRTRHKT AEQVKAEFVN AISKKSQDGQ T INENEFLE YYADVNATLP KEKEEYFIDI ILSTWGITSS VDYVSPERLN DLELILYEKI RQKTLPGEDE GRTVYRAFKY FD IAEKGVI DISQFKAALE KFGCVFDDKE IHALFSKFDV NRSGKICYDE FCGNFARLGS GNNPNVNPVF KLAREVPSPI INK LNADLK KQGPFAFRSL SKACQKADTF LNGSLSHDEF KWVIKEIGFS LTKTEFDNLF KFFDKNYEGK VIYSVFIDLV RGQQ SEIIT QSLSQGYDAL ASNNGGKVTV ESIVQRYNPL AIQIIYPTRK IAQDLQREFV AEWGGNLQQQ VNKNAFVEFF SDVRA FCDS ESKFHQYFSN AFGL

UniProtKB: EF-hand pair protein

Macromolecule #17: CFAP182A

• Macromolecule: Name: CFAP182A / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila (eukaryote) / Strain: CU428
• Molecular weight: Theoretical: 20.10984 KDa

Sequence

String:

MDKLLASQRI NNSFGCKAMY QTTNNDYGQP FLQDNKTVPT YNELVEAQTE VTRAEKQLKN KTVATKFSDL KVEDEDEIMK EKRKFDRNV IVPKKFPLKE DSYKAPEPGL KIGNPLYMTA NREYGMLKPT AFEIPNRFYP KDNTYSKSFP GMHKFDGLNT N ITFSKVHR ALDEY

UniProtKB: Uncharacterized protein

Macromolecule #18: CFAP143

• Macromolecule: Name: CFAP143 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila (eukaryote) / Strain: CU428
• Molecular weight: Theoretical: 30.485744 KDa

Sequence

String:

MELNQTTLES YNKQLQKGQG TLIGNWWEER ELRDVTGIGR SAHNYAKLKS TISQTGAQSL YKESPQTESV NDTNERTMGK KFNHIPNTT NSEYGKGFNK ADQLPRTGPV HLRTQQQMIN HIKQELNEIE NQKEIQRNIR YFQTTTQTEF GPKEHAMAGC T VGRRVMRT QNGQPITPDN RDEDILVDHG FLERQPFLTD EELKNQLPQG ESYLTQQPIT YWTEKTTDGF GCVYQSKSNP ND PKSTFKL NNQFLKTFHD YSHVRK

UniProtKB: Uncharacterized protein

Macromolecule #19: CFAP21A

• Macromolecule: Name: CFAP21A / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila (eukaryote) / Strain: CU428
• Molecular weight: Theoretical: 48.502359 KDa

Sequence

String:

MAANRTKDLF PGFKTAGAST KLPDESAMNC IKPKENLNPG TPEHIKKYRK SYKNQPGSTI LHYGIYDDQK PPETFVYGKK IEGSDHVQQ VMDSGKTDGI KQMINEIKEA KYHSRQVEPL GKRMERNYEF PQEVHQDNFK FGVATINSEN TAKEVMFPQK P AVNDQAAH DQYVRSHGNY EAGEQKNRNY NWKVDPQQHR FGKTDKIASN EVVYCLNQEA LQDQFPKTTI VQKKQEDFRN FK EDHLGLP KNLGQTNAKN NPDMVFGARL GGPDEWNAGK CISGEATLKE VQTDKDLGKT NRFGFRNITK DGDENRVFGV PTI RDDIQK PLMKSIADPN NYGDEKPAVN LLFPKKYDYM GVEQDDFKIK RHKYEIKDIF EKIGYKYKIG KFEGIFKRAQ EIEN STDNK VSCSSFLQAI QEMDHIE

UniProtKB: Uncharacterized protein

Macromolecule #20: CFAP53

• Macromolecule: Name: CFAP53 / type: protein_or_peptide / ID: 20 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila (eukaryote) / Strain: CU428
• Molecular weight: Theoretical: 60.717801 KDa

Sequence

String:

MILSQNQQFN YRNPNERAIY QRRVQDEKLD RLKTKLNQEF YEKEFACWEN RGKAVNEGNY IKQRLAELRV RSQRALNERR QKLATLLGR EHEQYKQEIH DLQETPEDIR QKMIQRVNHL KEEKEKERQK IVEEKREQQF VRNADELRKV DADFHELKTY H EQNIQIME KQKILQEKYE EEMIYAELYK YDIKKKEKEE LVKEYERKKK VDERNKVLHT QLAMNQTRRN QEKDLDSLEK MM LKEEWKK EEERHKKQQQ ELFQFNKDLN NEIKESNEEL QRRKAYEKDI EKQQDKEMIS RIITKEQMLD RLEAEQKRKF QEE TKAFLL NFKNRSNETH QYEAELERLI NEEAEKQWQR QNAIWQKERE AKIKLMHEVY DSRKTDIELK QTLVQVEKEQ KELE KDILT KAVAAYEQEV ERKKKELYLT NKQHQSSLIG QMHDKDRKRQ DELEKIRQEE EQERLRNIAY EQRLQEKKIQ GKQLL DELR KKRPF

UniProtKB: Cilia- and flagella-associated protein 53

Macromolecule #21: CFAP115

• Macromolecule: Name: CFAP115 / type: protein_or_peptide / ID: 21 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila (eukaryote) / Strain: CU428
• Molecular weight: Theoretical: 110.670203 KDa

Sequence

String:

MASVLAASGQ NILGAGRFNI SKNTITVNTD IEKLIKAEVF KKQIRIREFF YDFDRLRKGV VTEDKFRSAL SMLGMHLYEK DILSLINKY KSGPDQVKYT DFCDQIDAQF FDYNLAKENL ESTKSSSQIS QNESAIIQQI LLSIKQEIRT RRILLKQPFQ D FDRTRCSH ITVDQFSRVL TQLSILPNEQ LFNLLIRRYI DNGNPKEVNY VKFCDDVDNV SEMLETVIKG IKPNEKVFDP NE DIVEDTK DLKLMSTLFT SKKLNFGNKQ LNDVIVKIQA EVVMKRIRIR EFFKDYDNLR KGVVSEQQFR RVLDVSNIKL SEQ DINLLL SEFKVDNIPN GQVRYSDFCE VIDEIFTKKA IDKDPLATVT QFQPETTLPA RRYYLNLTEE EKGQLRHLLN LYHK EIQNK RVLIKPHFED FDKTKQGYIS KNQFLRILNQ FSLFPSEEAL NLILKRYTDK GTLDEVNYYE FCRDVDIYDE GVLVS KTYA DSFKNYQKPE KEFQPFIHND IPNDIEDLLA KLRAKVKEQR LRISEFLRDF DKLRSGNITK EQLRLGLSMA KLPLSD AEF TLVIENFAST TKENNVRWKD FCDQIDSVFT QKNLEKKSAT LPVFNPSTEY KYGRRGLTEQ ERRIAEHMKA RFRQFCQ AT RIDIKQFFQD WDRNGRNKVT PKQFRQVLTT VNFNISDPEF NAISRMYSSE DEHDVRYVDF IRDTKVYEQH LWSSNQTK D FGAHGLDTRK RPVDANVLLE EIKNIIKINR LRVGEYFADY DPLRKGVIPT NKFRGVISQM KIDLDEEQIL KLENMYILQ SDPTKLDYAS FLEDVNIVFT KTGLEKDPIA KPVPFEKKNA IDPRDVLTPQ EEEDLHVLLL RLEAVISKHR IMFKTHFQDK DIAKSGKVS FTRFRSILDL HKLPLTDQQF KLLCKRFAHQ GVEFNYIEFD EVVKKYENLY S

UniProtKB: EF hand protein

Macromolecule #22: Nucleoside diphosphate kinase

• Macromolecule: Name: Nucleoside diphosphate kinase / type: protein_or_peptide / ID: 22 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila (eukaryote) / Strain: CU428
• Molecular weight: Theoretical: 42.642727 KDa

Sequence

String:

MADIRYIFIV EWFDTAASLI RTYYLTYFTQ DKTIEMYDLK NKKVFLKRCE YAIKDSDLYI GSILNVYSRQ LKIVDFADVF TRSKFQNIK EKTFAMIKPD AYIHIGKIIS IIERSGLQIS NLKMTKMSQE DAREFYGEHK GKPFYDGLVN FMSSDLIVGM E LVGDNAIK RWRELLGPTN TLVAREQAPN SIRGLFGTDG TRNACHGSDS PGSAFRELNF FFAKTSKLKT QAFFNQCTCC VI KPHIVKQ NQVGEVIDMI LSEGFEISAL QTFFLDRPTA EEFYEVYKGV LPEFNAIAEH LTSGMCYALE VRQENAVKSF RDI AGPHDP EIAKVIRPNT IRARFGIDRV KNGIHCTDLE DDGVLEVEYF FNALQN

UniProtKB: Nucleoside diphosphate kinase

Macromolecule #23: Cilia- and flagella-associated protein 45

• Macromolecule: Name: Cilia- and flagella-associated protein 45 / type: protein_or_peptide / ID: 23 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila (eukaryote) / Strain: CU428
• Molecular weight: Theoretical: 59.580438 KDa

Sequence

String:

MPSSQASGGS KKSNADETLF GSRKTNKNNT KAQEEVVDKA RKGDFTHPEV VLIGESELQR MKNNAIITTK EETLHQKKIL EEQKEKQMA VSKAKKQKML QMEEEKKKQV PLSDFQLEEK VVKDSLLNRA QEIMNEQMDD VKEMNKMVMY AKCVTIRDKQ L TEKKDIRD EKKLEEKRKD LMMEIERLKK IKYYEEQEKK KKEEQKEGHL KIIDQIKENE LVRLRAKEEQ EREGQVMIKA IK ELEVEEQ HKALKKKSDQ KKLLDEIFEA NQNAILVKQK RVQEEKVEEE KIVKYNLEKA QKEAEYIAEQ KRIKDEKEKE LQR LREMQE KANDRQAELD FLRAKRAMEQ NERNARDKER REAELRQKIN QELLEARRQQ QYEKEQRLQE QAKNDRDEFQ RIIQ AQKQE REIEIKLQIE REQQIKKHAD ELRKQIALNE EKIKQDQRNR LEEGKKIRDQ LQQQKKMLES IKSQKLEQLQ KMNIP QKYT SELERKKINI

UniProtKB: Cilia- and flagella-associated protein 45

Macromolecule #24: CFAP210

• Macromolecule: Name: CFAP210 / type: protein_or_peptide / ID: 24 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila (eukaryote) / Strain: CU428
• Molecular weight: Theoretical: 61.707121 KDa

Sequence

String:

MSVQSQSRQN KSSYGSQRQG SIQQQSINQQ SRTSNNFNQS NKTFTQKNTI QVPFSEIVRM KENCQINSDP EKERKLKEKT ELYELSKQR VKNWPNTIQA IRKKKDETRF EKFKKDEEER RRVDEEEARY QANVKKEIIE KANKQIYEGN DRVKAFQSKL L LVDTLQER EGQIELKKQV QEMEKMREEI HLENMKENIR KLEEEEQRKK EELEEKKRHA QKILREQHEE MKVKYIKRLQ DE KIEGEII KQRALEAIAK EKEEERARRE KAIQIQIDTK KGNEVLQTIR AELKEKEKLE DEKIKQYAEK KEKITEMRRL REE MRFKEK QEQRQKLIDE QIKRLAAIKN EEESRINKQI IEAQMKADEA EKKKREAIAA QKKRFDESIQ LTLKRKEQQV VEQK MEDQA YQQYWQKKNV QIQQREHQEK REAFNRAKDL QDFHKVQANE KQKLREEAFL NEMDEAEKIK QRLENDDKNF NSWAE RMIR EWNSDGKDIK PLLKELATYN KKL

UniProtKB: Trichohyalin-plectin-homology domain-containing protein

Macromolecule #25: RIB72B

• Macromolecule: Name: RIB72B / type: protein_or_peptide / ID: 25 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila (eukaryote) / Strain: CU428
• Molecular weight: Theoretical: 60.972898 KDa

Sequence

String:

MASGFPKLPG FVPTQPIEQT SFKRVSAAKQ EYNRDYLHKN VPPVVYPLPR QVPKEPPSQK GMFNPNYMST THDTYRPVNC PNDVTELYQ PTWVKMDRQV LRFNAYFKES CVESSLEAYR VRKVVLFFYL EDSSIEISEP KQMNSGIPQG SFLKRQKVLK A DGSGVYLS LYDFQIGQDL EFFGKIFHIY DCDIYTREFY ENLGIPQGAS ELPDSDNWEK KTLNKFVPVK DHMLKDFMEH KL GGGRVPS QKQFLENDRK VLKFFVFSEI PFFLHYYLAD DTLEIREIHR QNSGRDPFPL YLKRQKLPKQ FALNQPGQTY AEN FVKPTD IKFGEDLVAF GKKFRINGCD KYTQDYYREK YNIDFPLGED YEMQYRDIVK REIPPYNGFG DEEDSLGYVY RLIP KPPRK DFFKWVDNQV TLRFNAKFNT NKPEDIHRRF IITYYLNDDS LQVYEPAIRN SGIPDGKFLE KNRYKNVQTN NEYFQ PTDL VVGKDVIING YSFRILECDE YTLKWFANNI VQ

UniProtKB: EF-hand protein

Macromolecule #26: Protofilament ribbon protein

• Macromolecule: Name: Protofilament ribbon protein / type: protein_or_peptide / ID: 26 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila (eukaryote) / Strain: CU428
• Molecular weight: Theoretical: 34.224398 KDa

Sequence

String:

MKELSQIIDK QISQLNLFGK IKKRKRQSNI YKMSGNTNSD FNRTNYQHKE QIIRCGISSL KCLDGEDLNQ GNRRRLQQLQ QRDWIEQQI REKEERKRQE DEEKKAFEQQ TLHINMMRGD LEDNLNQKRR NWEKNTKEFN IQQRNEKLDY ERSSHLDNQA Q NQYHITYC NTNNFQTENT GTCTSAFGPH RVIPYHWKGM NPQQKKDIIL EQDQQRHERE ILKNLERDED KAFSNQTEHN RF MLINLER QKNRQHRQRM DEIKEFNLLA AKEQKIKLKH MYD

UniProtKB: Protofilament ribbon protein

Macromolecule #27: RIB35

• Macromolecule: Name: RIB35 / type: protein_or_peptide / ID: 27 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila (eukaryote) / Strain: CU428
• Molecular weight: Theoretical: 34.952652 KDa

Sequence

String:

MSLYGVGLNV QVDRIKTEFR LYVSKQKGGL NVRNLRRIFE QLDYQQNGQL NLDDFKAGLN KFGFFLKNVD YQCLLKYFDV EQNGNVNYL AFLSCISQTL NQRRANFVLK VFDSIDSEQK GAVHPFSLAE RLEVVKNKDY QIGSKTREQL VGEYVAQFHK N QDGLITRD EFVSYYEDLG LGISSDQHFI EILQTQWGIK EDDDLAITKE EVKGIVKTIR QKLIQKTKGA NDEFLLRKLF KE FDISNSG FLTLDEMNAM LIKLEIPIQK RYLNAVFSQL DRNHSGVIEF EEFVYYIVND PYP

UniProtKB: EF-hand pair protein

Macromolecule #28: CFAP182B

• Macromolecule: Name: CFAP182B / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila (eukaryote) / Strain: CU428
• Molecular weight: Theoretical: 11.702059 KDa

Sequence

String:

MNYNNLTQEQ VQEQIKKLEE EHQDFHKKHA NTFFKTTYKT YGGEKQVIDV PKHFGHDGRF TDHLLHATMY RNHSLNTTCD KERWMKNSK DWMEHLN

UniProtKB: Uncharacterized protein

Macromolecule #29: Flagellar FliJ protein

• Macromolecule: Name: Flagellar FliJ protein / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila (eukaryote) / Strain: CU428
• Molecular weight: Theoretical: 12.098964 KDa

Sequence

String:

MINIQRSIKL NEKYQQAEQY SKKLEGISRI TNWHETKVKY DNANQNKYAR KMIDQEMKYA LKEVKMVRNV KLKELYEQEA KQWEQELSQ KGLAIYKHKH

UniProtKB: Flagellar FliJ protein

Macromolecule #30: RIB27B

• Macromolecule: Name: RIB27B / type: protein_or_peptide / ID: 30 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila (eukaryote) / Strain: CU428
• Molecular weight: Theoretical: 26.270043 KDa

Sequence

String:

MSQTENILDK KQNTTKQGID HSKFYEWSKQ FQYISSYQSN SSDKPNELRS DAIQGYKGFI PQIQSNNMFG QTFSRSARQV LASNVSNNP FKLSSTGHNS LRHNYADQSL NAFTHKFGAT TIQKNHPSLE SNLKQSLYKQ QFSKPVFSNE NNQDFNADDL N GNNNLSKK TILSGFMKNN SIVGFKECLD SQLQLKDSKS EYNDKFQSKN RIHRDTSIFH IRNKSQIIIP A

UniProtKB: Uncharacterized protein

Macromolecule #31: STPG2

• Macromolecule: Name: STPG2 / type: protein_or_peptide / ID: 31 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila (eukaryote) / Strain: CU428
• Molecular weight: Theoretical: 33.647141 KDa

Sequence

String:

MADKVEEEQA PPKIYTYIDL LKFENPEIYS SFGKMRLSHK TTAPKYGFGT ADRNKQAKVF QNKELAKTNF AGKSSPGPAY DVRDTDYFT YQKAPKWKIG SEVRNTLNTG SKHDFYLRKD VDFDPLEADI FRRPKAPTVR IGLELRFPND PKRHKGTPGP Q YNPTLRHE IPNPPKFSFG FRREIQGFSP LVANSSTPQL VGPGSYIQKN VPNTSKIRNE PKWSFPNAER FSGFQADLSN AH YTKPRAI GTQYDSRKQT LPMFSFGKST RESKRGTFKD MMSTQEVRIR ISMPKF

UniProtKB: Sperm-tail PG-rich repeat protein

Macromolecule #32: RIB26

• Macromolecule: Name: RIB26 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila (eukaryote) / Strain: CU428
• Molecular weight: Theoretical: 26.114875 KDa

Sequence

String:

MDDINKKVTT SKPSNVIFNT QTRGYCPGYL SPHNQISKKG LIVLQEWWGM NESICLLADE FAHQGFKVLV PDLYRGKVAK SHEEAGHLL TGLDWKGAIE DIAHALQYLI KLQGCTSVGI TGFCMGGALT LASLSAIEGF SCGSPFYGIC DQQTFPVTNI R VPVLAHFG EDDSMVGFSD VESAKALKLK AHEASVDFRL RTYPQAGHAF MRKESKTYQP QAAAAAFKET VEFFQQLLK

UniProtKB: Dienelactone hydrolase family protein

Macromolecule #33: CFAP129

• Macromolecule: Name: CFAP129 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila (eukaryote) / Strain: CU428
• Molecular weight: Theoretical: 31.385977 KDa

Sequence

String:

MISKVSGYQG FSSYGDKPYP SLKPGRQVLS PNDVWEQTQR TRDDASMYEN HQHYKTVYKV DVSNAVQPKV YQNHTHVKKG LQTQYQLQA TGKSVLSYGS DRKDQTFDPN NETSKLKTGV EHWKSNYNAN IKDPYSYSKA SRPEWSYHLK PHQVDSKIGP T EYKTTFGE FGTKPTDKLN EYGNEIINKH EDPLKMGTTK STFHIPNYTG FIPAARTVGK SLEHAAALNS RIDKSRATIV DN YHTKIPG YAGHQPKAPV NQRGFLREHC FSTVSSLKL

UniProtKB: Ptype ATPase, putative

Macromolecule #34: Flagellar microtubule protofilament ribbon protein

• Macromolecule: Name: Flagellar microtubule protofilament ribbon protein / type: protein_or_peptide / ID: 34 / Number of copies: 4 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila (eukaryote) / Strain: CU428
• Molecular weight: Theoretical: 72.643695 KDa

Sequence

String:

MNYNLPKTVP LLPGHCFPDH LKESHHKTQQ FTLVNNVHCE KTNYIEEKND PYLIDSLRMG TPPDLTYGKR KAPINDYIPR IQPPWLKYD RQVLRFYCYF QESVVENPYE NYRIRKCTLY YYLSDGTIHV NEPKIMNSGI EQGVFIKRQQ IPKQLNSTDY Y TWEDLNVG ININLFERVF RIVDCDSFTK EFFVYMGKKM NSPERVPNDA FEAQKTLKDI KIPPPNTKEY NEYNEVKLGG GH PNTGLQK YLENDRKVLS FNVLWNDTSL EGGLNYYILN YFLADDTTEI KEIKRHNSGK DAFPLFLCRK KLPKEPIMTH YPG MTLKKE EYYSPSDLVC GNMVRIYNKD CLIFNCDAYT KEWYLQNMNL QQIPITLKKE DIKRFYQPVP PYNGFGSEAD SLGS VYNLQ PKAPRKDINK MYTQDQYILR FEGKLISQNK EDNHRKFIIS FFCGDDTIMV YETADKNSGI WGGKFLERMN HNNPI NNKP YTELDFQIGE IIQLGVYRFQ LLRADEYTHK YMKSKPEVFK EADIEYTLNH LRKFATKYKS YDEFMVLLIK NIDPNR RGV IDFNDFVVGL RRLGYNLTYQ EIYTLMRYFD LDENWKLDVK SLFVALGGKQ

UniProtKB: Flagellar microtugule protofilament ribbon protein

Macromolecule #35: Parkin co-regulated protein PACRGB

• Macromolecule: Name: Parkin co-regulated protein PACRGB / type: protein_or_peptide / ID: 35 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila (eukaryote) / Strain: CU428
• Molecular weight: Theoretical: 28.41318 KDa

Sequence

String:

MQSLSPSRKV KPNNTTHLLN ISSKFTGTGG NCSSLAPKKV QPIPKVKKTG IFEAKAIEAV EINLFKKYYD RGDLPIAVNF HGAHRKISW KLEPELLDYH LYLPIFFEGL RETEEPYKFL ADQGCEELLA RGGAKIFAVL PQLIIPIKKA LSTKSHPIMC T TLKKIQKL VTSSDMIGEA LVPYYRQILP VMNMFKNKRL NIGDHIDYSQ RKNENLSDLI QETLEILEKY GGEDAYINIK YM IPTYESC MF

UniProtKB: Parkin co-regulated protein

Macromolecule #36: OJ2

• Macromolecule: Name: OJ2 / type: protein_or_peptide / ID: 36 / Number of copies: 4 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila (eukaryote) / Strain: CU428
• Molecular weight: Theoretical: 19.931387 KDa

Sequence

String:

MPPIDTKKSQ ITDFSQSTRL QYLGDKKSQK SAAFFRDTRV SSCSYISLKG GVPRAIPYYG SPTKTYADTM SKSSYISSLN HDTYRVRPY QHVGMSQKLL EPYHPHSYRN RLPVPDAPPQ FSNASQIEVG DRSEVNHRRF VSQSKNVYGN FGKFDPVSNP G ILASKTKW HHHLQSK

UniProtKB: Uncharacterized protein

Macromolecule #37: CFAP77A

• Macromolecule: Name: CFAP77A / type: protein_or_peptide / ID: 37 / Number of copies: 4 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila (eukaryote) / Strain: CU428
• Molecular weight: Theoretical: 28.989633 KDa

Sequence

String:

MSRTSTGFYP WNNRTQNTLL LKDDVGRSKP SAYSLPPVDY TYGRPLERDE EGAKEVSMTW KYHAETRPKE PDRDFAKLNK MSLTQGLHK SNQFSDYRKR VDARMKVVKG RNALPLYIPE EEFRYGLPNR PSTPIKLVLG NCYGLEFQQR IEQDYMYRSQ Q DSFKPRLL PKGNKASDLS YTYTKQKLSQ IQQNNNKELF KLTKFKKVQP RTNTYLHKNV TDVGKTSEIP NHEEHQHYDA NE VPNQAVE

UniProtKB: Sperm-tail PG-rich repeat protein

Macromolecule #38: OJ3

• Macromolecule: Name: OJ3 / type: protein_or_peptide / ID: 38 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila (eukaryote) / Strain: CU428
• Molecular weight: Theoretical: 14.31563 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

Macromolecule #39: SB1

• Macromolecule: Name: SB1 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila (eukaryote) / Strain: CU428
• Molecular weight: Theoretical: 17.793787 KDa

Sequence

String:

MIRDFVLQQT TEPEKKFNST VLIGNWYEER CDPNREQSKF YNERKFADNN YQKYTLSENK FQDTSNSWLN FQENKPEVKN DQFITMNMQ EYKKPSEQKR NSELKPFIVK KSHFDKNPHE LEEYREKWTK SAHTFDRMYL GTQKPN

UniProtKB: Uncharacterized protein

Macromolecule #40: STPG1A

• Macromolecule: Name: STPG1A / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila (eukaryote) / Strain: CU428
• Molecular weight: Theoretical: 42.132254 KDa

Sequence

String:

MQCLLLVFQF NQNYTVKKIA EKEILSNFYL QSRLIDQQNS SYLNMSQSLP SVQKSASMTL MPIMEMYNIS TRQHAAWGLD GYEVPKKYF DHLKVVQDRH FEEISKSGKA TKNNKIITKR GSYLEDEIKF RGQNPGPQKY DVTYKWVSDA EIEKGKKLPK N TKKNTFIE QIFLEQQRRG IPGPGKYNIL KTDEQVKAEA EKMNKKLKYG ERSNYLQEYE YLSSTLPGPG NYNPRPILPK IH KDNMSPD KWIAFHKAKL SKTAKSSLPD VGTYKMNYPL DYATFGKMLV KTQEEGGNKK SSVRYMGTEE RFKDPKKTKS KTS QIVPGP GQYPLVAKWQ GKEQKKDSKD KNWMDSITTG ISKSIYYS

UniProtKB: Sperm-tail PG-rich repeat protein

Macromolecule #41: Nebulin

• Macromolecule: Name: Nebulin / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila (eukaryote) / Strain: CU428
• Molecular weight: Theoretical: 61.047602 KDa

Sequence

String:

MTDNPQQPHK SKQEIQREQR KELARELRKA HFDLGFKEGF DDETRYREFY KWYDLEQSEK TKQEMLKLRN DLRSTHYILG TDDPNKLFV STATQSFVKP VNPQVSQLSV ETKNDLRSHH FNLGHYNDKV LSDYKLNYDQ KQIDPETLKD RKEQINFLRK H NHDFGDKN NYHSSMYNEN FNKSYDPHFL KQGKSKEEIH QQIVDLRKTN LVMGNNNPQF TSEAMSEFNN KPQAFRTQVD LG LKKSHFK LGEDPSLYET TTAKTYQGKQ MFQHDPEKIK ALSKDLRAEH FKLGNDPQSY TSEAAAKFKE FDKNSLTQQP DMS YLYRSH FNLEGFGGSN PVQHYVSNYK QNYEPKAAQK SEATRNDRAD RGSHIVFGSD KIDDQFKSEA QKNFVNFGRQ APSA LEKEV QADLRRHHYQ FGTDQPEMIS EMKKTFNDKT KESSQSKLDP NLIKDLRSNH FEYGTMGNEY TTTMQDIGRY QCQPS RLNP ELAKDLRSHH FRPGDLEKYY DTTYRLAFID FKAV

UniProtKB: Uncharacterized protein

Macromolecule #42: B5B6_fMIP

• Macromolecule: Name: B5B6_fMIP / type: protein_or_peptide / ID: 42 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila (eukaryote) / Strain: CU428
• Molecular weight: Theoretical: 43.166234 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

Macromolecule #43: CFAP112A

• Macromolecule: Name: CFAP112A / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila (eukaryote) / Strain: CU428
• Molecular weight: Theoretical: 45.038562 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)

Macromolecule #44: B2B3_fMIP

• Macromolecule: Name: B2B3_fMIP / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila (eukaryote) / Strain: CU428
• Molecular weight: Theoretical: 31.099145 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)AYA AA AYAYAAA AYAAAAYAAA AAAAAAAAAY AYAYAAAYAA AAAYAAAAAA YAAAAAAAYY AYAAAAYAAA AAAAAAAAAA AAA AAAAAY AAAYYYYAAA AAYAAYAAAY AAAAAAAYAA AYAAAAAAAA AAAAAAAAAA AAAAAYYAAA AAYAAAYAAA AAAA AAAAY AAAAAAYA(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)

Macromolecule #45: Tubulin alpha chain

• Macromolecule: Name: Tubulin alpha chain / type: protein_or_peptide / ID: 45 / Number of copies: 161 / Enantiomer: LEVO; EC number: Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement
• Source (natural): Organism: Tetrahymena thermophila (eukaryote) / Strain: CU428
• Molecular weight: Theoretical: 49.639035 KDa

Sequence

String:

MREVISIHVG QGGIQVGNAC WELFCLEHGI QPDGQMPSDK TIGGGDDAFN TFFSETGAGK HVPRAVFLDL EPTVIDEVRT GTYRQLFHP EQLISGKEDA ANNFARGHYT IGKEIVDLCL DRIRKLADNC TGLQGFLVFN SVGGGTGSGL GSLLLERLSV D YGKKSKLG FTIYPSPQVS TAVVEPYNSI LSTHSLLEHT DVAVMLDNEA IYDICRRNLD IERPTYTNLN RLIAQVISSL TA SLRFDGA LNVDITEFQT NLVPYPRIHF MLSSYAPIIS AEKAYHEQLS VAEITNSAFE PANMMAKCDP RHGKYMACSM MYR GDVVPK DVNASIATIK TKRTIQFVDW CPTGFKVGIN YQPPTVVPGG DLAKVMRAVC MISNSTAIAE VFSRLDHKFD LMYA KRAFV HWYVGEGMEE GEFSEAREDL AALEKDYEEV GIETAEGEGE EEGY

UniProtKB: Tubulin alpha chain

Macromolecule #46: Tubulin beta chain

• Macromolecule: Name: Tubulin beta chain / type: protein_or_peptide / ID: 46 / Number of copies: 161 / Enantiomer: LEVO
• Source (natural): Organism: Tetrahymena thermophila (eukaryote) / Strain: CU428
• Molecular weight: Theoretical: 49.617676 KDa

Sequence

String:

MREIVHIQGG QCGNQIGAKF WEVISDEHGI DPTGTYHGDS DLQLERINVY YNEATGGRYV PRAILMDLEP GTMDSVRAGP FGQLFRPDN FVFGQTGAGN NWAKGHYTEG AELIDSVLDV VRKEAEGCDC LQGFQITHSL GGGTGSGMGT LLISKVREEY P DRIMETFS VVPSPKVSDT VVEPYNATLS VHQLVENADE CMVIDNEALY DICFRTLKLT TPTYGDLNHL VSAAMSGVTC CL RFPGQLN SDLRKLAVNL IPFPRLHFFM IGFAPLTSRG SQQYRALTVP ELTQQMFDAK NMMCAADPRH GRYLTASALF RGR MSTKEV DEQMLNVQNK NSSYFVEWIP NNIKSSICDI PPKGLKMAVT FVGNSTAIQE MFKRVAEQFT AMFRRKAFLH WYTG EGMDE MEFTEAESNM NDLVSEYQQY QDATAEEEGE FEEEEGEN

UniProtKB: Tubulin beta chain

Macromolecule #47: GUANOSINE-5'-TRIPHOSPHATE

• Macromolecule: Name: GUANOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 47 / Number of copies: 161 / Formula: GTP
• Molecular weight: Theoretical: 523.18 Da

Chemical component information

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

Macromolecule #48: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 48 / Number of copies: 161 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Macromolecule #49: GUANOSINE-5'-DIPHOSPHATE

• Macromolecule: Name: GUANOSINE-5'-DIPHOSPHATE / type: ligand / ID: 49 / Number of copies: 161 / Formula: GDP
• Molecular weight: Theoretical: 443.201 Da

Chemical component information

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: cell

Sample preparation

• Concentration: 2.2 mg/mL
• Buffer: pH: 7.4
• Vitrification: Cryogen name: ETHANE

Electron microscopy

• Microscope: TFS KRIOS
• Image recording: Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 45.0 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 3.0 µm / Nominal defocus min: 1.0 µm
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

Startup model

Type of model: EMDB MAP
EMDB ID:

8537

• Final reconstruction: Applied symmetry - Point group: C₁ (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 4.1 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 148365
• Initial angle assignment: Type: NOT APPLICABLE
• Final angle assignment: Type: MAXIMUM LIKELIHOOD