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- EMDB-29658: Mutant bacteriophage T4 gp41 helicase hexamer bound with single s... -

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Basic information

Entry
Database: EMDB / ID: EMD-29658
TitleMutant bacteriophage T4 gp41 helicase hexamer bound with single strand DNA and ATPgammaS in the stalled primosome
Map data
Sample
  • Complex: Complex of mutated T4 bacteriophage helicase gp41 with ssDNA/RNA hybrid
    • Protein or peptide: DnaB-like replicative helicase
    • DNA: DNA (5'-D(P*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*T)-3')
  • Ligand: PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER
  • Ligand: MAGNESIUM ION
Keywordsphage / complex / helicase / REPLICATION
Function / homology
Function and homology information


bidirectional double-stranded viral DNA replication / DNA helicase activity / Hydrolases; Acting on acid anhydrides; Acting on acid anhydrides to facilitate cellular and subcellular movement / DNA replication / hydrolase activity / DNA binding / ATP binding
Similarity search - Function
Bacteriophage T4 DnaB-like replicative helicase / DnaB-like helicase C terminal domain / DNA helicase, DnaB-like, C-terminal / Superfamily 4 helicase domain profile. / P-loop containing nucleoside triphosphate hydrolase
Similarity search - Domain/homology
DnaB-like replicative helicase
Similarity search - Component
Biological speciesEscherichia phage T4 (virus)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.61 Å
AuthorsFeng X / Li H
Funding support United States, 2 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)GM131754 United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)GM013306 United States
Citation
Journal: Nat Commun / Year: 2023
Title: Structural basis of the T4 bacteriophage primosome assembly and primer synthesis.
Authors: Feng X / Spiering MM / de Luna Almeida Santos R / Benkovic SJ / Li H
#1: Journal: Biorxiv / Year: 2023
Title: Structural basis of the T4 bacteriophage primosome assembly and primer synthesis.
Authors: Feng X / Spiering MM / de Luna Almeida Santos R / Benkovic SJ / Li H
History
DepositionFeb 1, 2023-
Header (metadata) releaseJun 14, 2023-
Map releaseJun 14, 2023-
UpdateAug 23, 2023-
Current statusAug 23, 2023Processing site: RCSB / Status: Released

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Structure visualization

Downloads & links

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Map

FileDownload / File: emd_29658.map.gz / Format: CCP4 / Size: 30.5 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Voxel sizeX=Y=Z: 1.029 Å
Density
Contour LevelBy AUTHOR: 0.02
Minimum - Maximum-0.21607828 - 0.27765292
Average (Standard dev.)-0.000000484475 (±0.00897432)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions200200200
Spacing200200200
CellA=B=C: 205.8 Å
α=β=γ: 90.0 °

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Supplemental data

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Sample components

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Entire : Complex of mutated T4 bacteriophage helicase gp41 with ssDNA/RNA ...

EntireName: Complex of mutated T4 bacteriophage helicase gp41 with ssDNA/RNA hybrid
Components
  • Complex: Complex of mutated T4 bacteriophage helicase gp41 with ssDNA/RNA hybrid
    • Protein or peptide: DnaB-like replicative helicase
    • DNA: DNA (5'-D(P*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*T)-3')
  • Ligand: PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER
  • Ligand: MAGNESIUM ION

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Supramolecule #1: Complex of mutated T4 bacteriophage helicase gp41 with ssDNA/RNA ...

SupramoleculeName: Complex of mutated T4 bacteriophage helicase gp41 with ssDNA/RNA hybrid
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#2
Source (natural)Organism: Escherichia phage T4 (virus)

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Macromolecule #1: DnaB-like replicative helicase

MacromoleculeName: DnaB-like replicative helicase / type: protein_or_peptide / ID: 1 / Details: mutant Bacteriophage T4 / Number of copies: 6 / Enantiomer: LEVO
EC number: Hydrolases; Acting on acid anhydrides; Acting on acid anhydrides to facilitate cellular and subcellular movement
Source (natural)Organism: Escherichia phage T4 (virus)
Molecular weightTheoretical: 48.796711 KDa
Recombinant expressionOrganism: Escherichia coli BL21(DE3) (bacteria)
SequenceString: MVEIILSHLI FDQAYFSKVW PYMDSEYFES GPAKNTFKLI KSHVNEYHSV PSINALNVAL ENSSFTETEY SGVKTLISKL ADSPEDHSW LVKETEKYVQ QRAMFNATSK IIEIQTNAEL PPEKRNKKMP DVGAIPDIMR QALSISFDSY VGHDWMDDYE A RWLSYMNK ...String:
MVEIILSHLI FDQAYFSKVW PYMDSEYFES GPAKNTFKLI KSHVNEYHSV PSINALNVAL ENSSFTETEY SGVKTLISKL ADSPEDHSW LVKETEKYVQ QRAMFNATSK IIEIQTNAEL PPEKRNKKMP DVGAIPDIMR QALSISFDSY VGHDWMDDYE A RWLSYMNK ARKVPFKLRI LNKITKGGAE TGTLNVLMAG VNVGKSLGLC SLAADYLQLG HNVLYISMQM AEEVCAKRID AN MLDVSLD DIDDGHISYA EYKGKMEKWR EKSTLGRLIV KQYPTGGADA NTFRSLLNEL KLKKNFVPTI IIVDYLGICK SCR IRVYSE NSYTTVKAIA EELRALAVET ETVLWTAAQV GKQAWDSSDV NMSDIAESAG LPATADFMLA VIETEELAAA EQQL IKQIK SRYGDKNKWN KFLMGVQKGN QKWVEIE

UniProtKB: DnaB-like replicative helicase

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Macromolecule #2: DNA (5'-D(P*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*T)-3')

MacromoleculeName: DNA (5'-D(P*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*T)-3') / type: dna / ID: 2 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: Escherichia phage T4 (virus)
Molecular weightTheoretical: 3.605356 KDa
SequenceString:
(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT) (DT)(DT)

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Macromolecule #3: PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER

MacromoleculeName: PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER / type: ligand / ID: 3 / Number of copies: 5 / Formula: AGS
Molecular weightTheoretical: 523.247 Da
Chemical component information

ChemComp-AGS:
PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER / ATP-gamma-S, energy-carrying molecule analogue*YM

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Macromolecule #4: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 4 / Number of copies: 5 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.8
Component:
ConcentrationFormulaName
20.0 mMHEPESHEPES
100.0 mMNaClsodium chloride
10.0 mMMgCl2Magnesium chloride
2.0 mMDTTDTT

Details: 20 mM HEPES pH 7.8, 100 mM NaCl, 10 mM MgCl2 and 2 mM DTT
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 66.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 1.0 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Startup modelType of model: PDB ENTRY
PDB model - PDB ID:

6qem

Final reconstructionAlgorithm: FOURIER SPACE / Resolution.type: BY AUTHOR / Resolution: 3.61 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 3.1) / Number images used: 272868
Initial angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC (ver. 3.3.1)
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3.1)
Final 3D classificationNumber classes: 3 / Avg.num./class: 800000 / Software - Name: RELION (ver. 3.1)

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Atomic model buiding 1

RefinementSpace: REAL / Protocol: OTHER
Output model

PDB-8g0z:
Mutant bacteriophage T4 gp41 helicase hexamer bound with single strand DNA and ATPgammaS in the stalled primosome

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