National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)
AI147884
米国
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)
AI141002
米国
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)
AI127193
米国
引用
ジャーナル: Nature / 年: 2023 タイトル: Cryo-EM structure of SARS-CoV-2 postfusion spike in membrane. 著者: Wei Shi / Yongfei Cai / Haisun Zhu / Hanqin Peng / Jewel Voyer / Sophia Rits-Volloch / Hong Cao / Megan L Mayer / Kangkang Song / Chen Xu / Jianming Lu / Jun Zhang / Bing Chen / 要旨: The entry of SARS-CoV-2 into host cells depends on the refolding of the virus-encoded spike protein from a prefusion conformation, which is metastable after cleavage, to a lower-energy stable ...The entry of SARS-CoV-2 into host cells depends on the refolding of the virus-encoded spike protein from a prefusion conformation, which is metastable after cleavage, to a lower-energy stable postfusion conformation. This transition overcomes kinetic barriers for fusion of viral and target cell membranes. Here we report a cryogenic electron microscopy (cryo-EM) structure of the intact postfusion spike in a lipid bilayer that represents the single-membrane product of the fusion reaction. The structure provides structural definition of the functionally critical membrane-interacting segments, including the fusion peptide and transmembrane anchor. The internal fusion peptide forms a hairpin-like wedge that spans almost the entire lipid bilayer and the transmembrane segment wraps around the fusion peptide at the last stage of membrane fusion. These results advance our understanding of the spike protein in a membrane environment and may guide development of intervention strategies.
全体 : local map of SRAS-CoV-2 postfusion spike protein in nanodisc from...
全体
名称: local map of SRAS-CoV-2 postfusion spike protein in nanodisc from data set I
要素
複合体: local map of SRAS-CoV-2 postfusion spike protein in nanodisc from data set I
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超分子 #1: local map of SRAS-CoV-2 postfusion spike protein in nanodisc from...
超分子
名称: local map of SRAS-CoV-2 postfusion spike protein in nanodisc from data set I タイプ: complex / ID: 1 / 親要素: 0 / 含まれる分子: #1 詳細: local map of SARS-CoV-2 postfusion spike protein in nanodisc from data set I
由来(天然)
生物種: Severe acute respiratory syndrome coronavirus 2 (ウイルス)
分子量
理論値: 210 KDa
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実験情報
-
構造解析
手法
クライオ電子顕微鏡法
解析
単粒子再構成法
試料の集合状態
particle
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試料調製
濃度
1.5 mg/mL
緩衝液
pH: 7.5 構成要素:
濃度
式
名称
0.15 mol/L
NaCl
sodium chloride
0.025 mol/L
Tris-HCl
Tris hydrochloride
凍結
凍結剤: ETHANE / チャンバー内湿度: 100 % / チャンバー内温度: 277.15 K / 装置: FEI VITROBOT MARK IV
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電子顕微鏡法
顕微鏡
FEI TITAN KRIOS
撮影
フィルム・検出器のモデル: GATAN K3 BIOQUANTUM (6k x 4k) 平均電子線量: 1.01 e/Å2