National Institutes of Health/National Institute of Environmental Health Sciences (NIH/NIEHS)
ZIA ES103247
米国
National Institutes of Health/National Institute of Environmental Health Sciences (NIH/NIEHS)
1ZIC ES102488
米国
National Institutes of Health/National Institute of Environmental Health Sciences (NIH/NIEHS)
1ZIC ES103206
米国
National Institutes of Health/National Institute of Environmental Health Sciences (NIH/NIEHS)
1ZIC ES102487
米国
National Institutes of Health/National Institute of Environmental Health Sciences (NIH/NIEHS)
1ZI ES043010
米国
National Institutes of Health/National Institute of Environmental Health Sciences (NIH/NIEHS)
1ZIC ES103326
米国
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
1K99-GM143534
米国
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
R35-GM136435
米国
引用
ジャーナル: Nat Struct Mol Biol / 年: 2023 タイトル: Structural basis for pre-tRNA recognition and processing by the human tRNA splicing endonuclease complex. 著者: Cassandra K Hayne / Kevin John U Butay / Zachary D Stewart / Juno M Krahn / Lalith Perera / Jason G Williams / Robert M Petrovitch / Leesa J Deterding / A Gregory Matera / Mario J Borgnia / Robin E Stanley / 要旨: Throughout bacteria, archaea and eukarya, certain tRNA transcripts contain introns. Pre-tRNAs with introns require splicing to form the mature anticodon stem loop. In eukaryotes, tRNA splicing is ...Throughout bacteria, archaea and eukarya, certain tRNA transcripts contain introns. Pre-tRNAs with introns require splicing to form the mature anticodon stem loop. In eukaryotes, tRNA splicing is initiated by the heterotetrameric tRNA splicing endonuclease (TSEN) complex. All TSEN subunits are essential, and mutations within the complex are associated with a family of neurodevelopmental disorders known as pontocerebellar hypoplasia (PCH). Here, we report cryo-electron microscopy structures of the human TSEN-pre-tRNA complex. These structures reveal the overall architecture of the complex and the extensive tRNA binding interfaces. The structures share homology with archaeal TSENs but contain additional features important for pre-tRNA recognition. The TSEN54 subunit functions as a pivotal scaffold for the pre-tRNA and the two endonuclease subunits. Finally, the TSEN structures enable visualization of the molecular environments of PCH-causing missense mutations, providing insight into the mechanism of pre-tRNA splicing and PCH.