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- EMDB-27441: CryoEM structure of Influenza A virus A/Melbourne/1/1946 (H1N1) h... -
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Open data
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Basic information
Entry | ![]() | |||||||||
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Title | CryoEM structure of Influenza A virus A/Melbourne/1/1946 (H1N1) hemagglutinin bound to CR6261 Fab | |||||||||
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![]() | NIAID hemagglutinin stalk binding antibody / Structural Genomics / Seattle Structural Genomics Center for Infectious Disease / SSGCID / VIRAL PROTEIN / VIRAL PROTEIN-IMMUNE SYSTEM complex | |||||||||
Biological species | ![]() ![]() ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 2.62 Å | |||||||||
![]() | Seattle Structural Genomics Center for Infectious Disease (SSGCID) | |||||||||
Funding support | ![]()
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![]() | ![]() Title: CryoEM structure of Influenza A virus A/Melbourne/1/1946 (H1N1) hemagglutinin bound to CR6261 Fab Authors: Yang M / Edwards TE / Horanyi PS / Lorimer DD | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 624.4 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 20.5 KB 20.5 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 19.4 KB | Display | ![]() |
Images | ![]() | 56.6 KB | ||
Others | ![]() ![]() | 614.8 MB 614.8 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 928.9 KB | Display | ![]() |
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Full document | ![]() | 928.5 KB | Display | |
Data in XML | ![]() | 28.2 KB | Display | |
Data in CIF | ![]() | 37 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||
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Voxel size | X=Y=Z: 0.675 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: #1
File | emd_27441_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #2
File | emd_27441_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
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Sample components
-Entire : CryoEM structure of Influenza A virus A/Melbourne/1/1946 (H1N1) h...
Entire | Name: CryoEM structure of Influenza A virus A/Melbourne/1/1946 (H1N1) hemagglutinin bound to CR6261 Fab |
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Components |
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-Supramolecule #1: CryoEM structure of Influenza A virus A/Melbourne/1/1946 (H1N1) h...
Supramolecule | Name: CryoEM structure of Influenza A virus A/Melbourne/1/1946 (H1N1) hemagglutinin bound to CR6261 Fab type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#4 |
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Molecular weight | Theoretical: 450 KDa |
-Macromolecule #1: Hemagglutinin HA1 chain
Macromolecule | Name: Hemagglutinin HA1 chain / type: protein_or_peptide / ID: 1 / Number of copies: 3 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Theoretical: 40.34752 KDa |
Recombinant expression | Organism: ![]() |
Sequence | String: MVLVNQSHQG FNKEHTSKMV SAIVLYVLLA AAAHSAFAGS DTICIGYHAN NSTDTVDTVL EKNVTVTHSV NLLEDSHNGK LCRLKGIAP LQLGKCNIAG WILGNPECDS LLPASSWSYI VETPNSKNGI CYPGDFIDYE ELREQLSSVS SFERFEIFPK E SSWPKHST ...String: MVLVNQSHQG FNKEHTSKMV SAIVLYVLLA AAAHSAFAGS DTICIGYHAN NSTDTVDTVL EKNVTVTHSV NLLEDSHNGK LCRLKGIAP LQLGKCNIAG WILGNPECDS LLPASSWSYI VETPNSKNGI CYPGDFIDYE ELREQLSSVS SFERFEIFPK E SSWPKHST TKGVTAACSH AGKSSFYRNL LWLTKKEDSY PKLSNSYVNK KGKEVLVLWG VHHPSSSKEQ QTLYQNENAY VS VVSSNYN RRFIPEIAER PEVKDQAGRI NYYWTLLEPG DTIIFEANGN LVAPWYAFAL SRGFGSGIIT SNASMHECNT KCQ TPQGAI NSSLPFQNIH PVTIGECPKY VKSAKLRMVT GLRNIPSI |
-Macromolecule #2: Hemagglutinin HA2 chain
Macromolecule | Name: Hemagglutinin HA2 chain / type: protein_or_peptide / ID: 2 / Number of copies: 3 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Theoretical: 23.845508 KDa |
Recombinant expression | Organism: ![]() |
Sequence | String: GLFGAIAGFI EGGWTGMIDG WYGYHHQNEQ GSGYAADQKS TQNAINGITN KVNSVIEKMN TQFTAVGKEF NNLEKRMENL NKKVDDGFL DIWTYNAELL VLLENERTLD FHDSNVKNLY EKVKIQLKNN AKEIGNGCFE FYHKCDNECM ESVRNGTYDY P KYSKEFLV ...String: GLFGAIAGFI EGGWTGMIDG WYGYHHQNEQ GSGYAADQKS TQNAINGITN KVNSVIEKMN TQFTAVGKEF NNLEKRMENL NKKVDDGFL DIWTYNAELL VLLENERTLD FHDSNVKNLY EKVKIQLKNN AKEIGNGCFE FYHKCDNECM ESVRNGTYDY P KYSKEFLV PRGSPGSGYI PEAPRDGQAY VRKDGEWVLL STFLGHHHHH H |
-Macromolecule #3: CR6261 Fab heavy chain
Macromolecule | Name: CR6261 Fab heavy chain / type: protein_or_peptide / ID: 3 / Number of copies: 3 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 27.066621 KDa |
Recombinant expression | Organism: ![]() |
Sequence | String: MEWSWVFLFF LSVTTGVHSE VQLVESGAEV KKPGSSVKVS CKASGGPFRS YAISWVRQAP GQGPEWMGGI IPIFGTTKYA PKFQGRVTI TADDFAGTVY MELSSLRSED TAMYYCAKHM GYQVRETMDV WGKGTTVTVS SASTKGPSVF PLAPSSKSTS G GTAALGCL ...String: MEWSWVFLFF LSVTTGVHSE VQLVESGAEV KKPGSSVKVS CKASGGPFRS YAISWVRQAP GQGPEWMGGI IPIFGTTKYA PKFQGRVTI TADDFAGTVY MELSSLRSED TAMYYCAKHM GYQVRETMDV WGKGTTVTVS SASTKGPSVF PLAPSSKSTS G GTAALGCL VKDYFPEPVT VSWNSGALTS GVHTFPAVLQ SSGLYSLSSV VTVPSSSLGT QTYICNVNHK PSNTKVDKRV EP KSCDKHH HHHH |
-Macromolecule #4: CR6261 Fab light chain
Macromolecule | Name: CR6261 Fab light chain / type: protein_or_peptide / ID: 4 / Number of copies: 3 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 25.514395 KDa |
Recombinant expression | Organism: ![]() |
Sequence | String: MEWSWVFLFF LSVTTGVHSQ SVLTQPPSVS AAPGQKVTIS CSGSSSNIGN DYVSWYQQLP GTAPKLLIYD NNKRPSGIPD RFSGSKSGT SATLGITGLQ TGDEANYYCA TWDRRPTAYV VFGGGTKLTV LGAAAGQPKA APSVTLFPPS SEELQANKAT L VCLISDFY ...String: MEWSWVFLFF LSVTTGVHSQ SVLTQPPSVS AAPGQKVTIS CSGSSSNIGN DYVSWYQQLP GTAPKLLIYD NNKRPSGIPD RFSGSKSGT SATLGITGLQ TGDEANYYCA TWDRRPTAYV VFGGGTKLTV LGAAAGQPKA APSVTLFPPS SEELQANKAT L VCLISDFY PGAVTVAWKA DSSPVKAGVE TTTPSKQSNN KYAASSYLSL TPEQWKSHRS YSCQVTHEGS TVEKTVAPTE CS |
-Macromolecule #7: 2-acetamido-2-deoxy-beta-D-glucopyranose
Macromolecule | Name: 2-acetamido-2-deoxy-beta-D-glucopyranose / type: ligand / ID: 7 / Number of copies: 6 / Formula: NAG |
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Molecular weight | Theoretical: 221.208 Da |
Chemical component information | ![]() ChemComp-NAG: |
-Macromolecule #8: water
Macromolecule | Name: water / type: ligand / ID: 8 / Number of copies: 61 / Formula: HOH |
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Molecular weight | Theoretical: 18.015 Da |
Chemical component information | ![]() ChemComp-HOH: |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Concentration | 0.5 mg/mL | |||||||||
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Buffer | pH: 8 Component:
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Grid | Model: EMS Lacey Carbon / Material: COPPER / Mesh: 300 / Support film - Material: GRAPHENE OXIDE / Support film - topology: LACEY | |||||||||
Vitrification | Cryogen name: ETHANE / Chamber humidity: 90 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Number grids imaged: 1 / Number real images: 9179 / Average exposure time: 2.4 sec. / Average electron dose: 80.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 2.5 µm / Nominal defocus min: 1.0 µm / Nominal magnification: 130000 |
Sample stage | Cooling holder cryogen: NITROGEN |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |