- EMDB-27326: Structure of hemolysin A secretion system HlyB/D complex, ATP-bound -
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基本情報
登録情報
データベース: EMDB / ID: EMD-27326
タイトル
Structure of hemolysin A secretion system HlyB/D complex, ATP-bound
マップデータ
試料
複合体: Membrane protein complex of HlyB and HlyD
タンパク質・ペプチド: Alpha-hemolysin translocation ATP-binding protein HlyB
タンパク質・ペプチド: Membrane fusion protein (MFP) family protein
リガンド: ADENOSINE-5'-TRIPHOSPHATE
リガンド: MAGNESIUM ION
キーワード
hydrolase / transport / MEMBRANE PROTEIN
機能・相同性
機能・相同性情報
type I protein secretion system complex / protein secretion by the type I secretion system / ATPase-coupled lipid transmembrane transporter activity / protein secretion / ABC-type transporter activity / peptidase activity / ATP hydrolysis activity / proteolysis / ATP binding / plasma membrane 類似検索 - 分子機能
Secretion protein HlyD, conserved site / Type I secretion membrane fusion protein, HlyD family / HlyD family secretion proteins signature. / HlyD family secretion protein / ATPase, type I secretion system, HlyB / Peptidase C39-like A / Peptidase C39 family / Peptidase C39, bacteriocin processing / Peptidase family C39 domain profile. / Type 1 protein exporter ...Secretion protein HlyD, conserved site / Type I secretion membrane fusion protein, HlyD family / HlyD family secretion proteins signature. / HlyD family secretion protein / ATPase, type I secretion system, HlyB / Peptidase C39-like A / Peptidase C39 family / Peptidase C39, bacteriocin processing / Peptidase family C39 domain profile. / Type 1 protein exporter / ABC transporter transmembrane region / ABC transporter type 1, transmembrane domain / ABC transporter integral membrane type-1 fused domain profile. / ABC transporter type 1, transmembrane domain superfamily / ABC transporter-like, conserved site / ABC transporters family signature. / ABC transporter / ABC transporter-like, ATP-binding domain / ATP-binding cassette, ABC transporter-type domain profile. / ATPases associated with a variety of cellular activities / AAA+ ATPase domain / P-loop containing nucleoside triphosphate hydrolase 類似検索 - ドメイン・相同性
Membrane fusion protein (MFP) family protein / Alpha-hemolysin translocation ATP-binding protein HlyB 類似検索 - 構成要素
ジャーナル: Cell / 年: 2022 タイトル: The hemolysin A secretion system is a multi-engine pump containing three ABC transporters. 著者: Hongtu Zhao / James Lee / Jue Chen / 要旨: Type 1 secretion systems (T1SSs) are widespread in pathogenic Gram-negative bacteria, extruding protein substrates following synthesis of the entire polypeptide. The Escherichia coli hemolysin A ...Type 1 secretion systems (T1SSs) are widespread in pathogenic Gram-negative bacteria, extruding protein substrates following synthesis of the entire polypeptide. The Escherichia coli hemolysin A secretion system has long been considered a prototype in structural and mechanistic studies of T1SSs. Three membrane proteins-an inner membrane ABC transporter HlyB, an adaptor protein HlyD, and an outer membrane porin TolC-are required for secretion. However, the stoichiometry and structure of the complex are unknown. Here, cryo-electron microscopy (cryo-EM) structures determined in two conformations reveal that the inner membrane complex is a hetero-dodecameric assembly comprising three HlyB homodimers and six HlyD subunits. Functional studies indicate that oligomerization of HlyB and HlyD is essential for protein secretion and that polypeptides translocate through a canonical ABC transporter pathway in HlyB. Our data suggest that T1SSs entail three ABC transporters, one that functions as a protein channel and two that allosterically power the translocation process.