+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-26931 | |||||||||
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Title | Negative stain map of cH4/3 state 3 | |||||||||
Map data | Negative stain map of cH4/3 state 3 | |||||||||
Sample |
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Keywords | Hemagglutinin / chimeric HA / broadly neutralizing stem antibody / CR9114 / IMMUNE SYSTEM | |||||||||
Biological species | Influenza A virus | |||||||||
Method | single particle reconstruction / negative staining / Resolution: 20.0 Å | |||||||||
Authors | Han J / Ward AB | |||||||||
Funding support | United States, 1 items
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Citation | Journal: Proc Natl Acad Sci U S A / Year: 2022 Title: Influenza chimeric hemagglutinin structures in complex with broadly protective antibodies to the stem and trimer interface. Authors: Xueyong Zhu / Julianna Han / Weina Sun / Eduard Puente-Massaguer / Wenli Yu / Peter Palese / Florian Krammer / Andrew B Ward / Ian A Wilson / Abstract: Influenza virus hemagglutinin (HA) has been the primary target for influenza vaccine development. Broadly protective antibodies targeting conserved regions of the HA unlock the possibility of ...Influenza virus hemagglutinin (HA) has been the primary target for influenza vaccine development. Broadly protective antibodies targeting conserved regions of the HA unlock the possibility of generating universal influenza immunity. Two group 2 influenza A chimeric HAs, cH4/3 and cH15/3, were previously designed to elicit antibodies to the conserved HA stem. Here, we show by X-ray crystallography and negative-stain electron microscopy that a broadly protective antistem antibody can stably bind to cH4/3 and cH15/3 HAs, thereby validating their potential as universal vaccine immunogens. Furthermore, flexibility was observed in the head domain of the chimeric HA structures, suggesting that antibodies could also potentially interact with the head interface epitope. Our structural and binding studies demonstrated that a broadly protective antihead trimeric interface antibody could indeed target the more open head domain of the cH15/3 HA trimer. Thus, in addition to inducing broadly protective antibodies against the conserved HA stem, chimeric HAs may also be able to elicit antibodies against the conserved trimer interface in the HA head domain, thereby increasing the vaccine efficacy. | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_26931.map.gz | 11.9 MB | EMDB map data format | |
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Header (meta data) | emd-26931-v30.xml emd-26931.xml | 12.4 KB 12.4 KB | Display Display | EMDB header |
Images | emd_26931.png | 43.4 KB | ||
Filedesc metadata | emd-26931.cif.gz | 3.8 KB | ||
Others | emd_26931_half_map_1.map.gz emd_26931_half_map_2.map.gz | 11.9 MB 11.9 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-26931 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-26931 | HTTPS FTP |
-Validation report
Summary document | emd_26931_validation.pdf.gz | 688.6 KB | Display | EMDB validaton report |
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Full document | emd_26931_full_validation.pdf.gz | 688.2 KB | Display | |
Data in XML | emd_26931_validation.xml.gz | 9.6 KB | Display | |
Data in CIF | emd_26931_validation.cif.gz | 11.2 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-26931 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-26931 | HTTPS FTP |
-Related structure data
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_26931.map.gz / Format: CCP4 / Size: 15.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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Annotation | Negative stain map of cH4/3 state 3 | ||||||||||||||||||||||||||||||||||||
Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.77 Å | ||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: Negative stain half map of cH4/3 state 3
File | emd_26931_half_map_1.map | ||||||||||||
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Annotation | Negative stain half map of cH4/3 state 3 | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: Negative stain half map of cH4/3 state 3
File | emd_26931_half_map_2.map | ||||||||||||
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Annotation | Negative stain half map of cH4/3 state 3 | ||||||||||||
Projections & Slices |
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Density Histograms |
-Sample components
-Entire : Negative stain map of cH4/3 state 3
Entire | Name: Negative stain map of cH4/3 state 3 |
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Components |
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-Supramolecule #1: Negative stain map of cH4/3 state 3
Supramolecule | Name: Negative stain map of cH4/3 state 3 / type: organelle_or_cellular_component / ID: 1 / Parent: 0 |
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Source (natural) | Organism: Influenza A virus |
-Experimental details
-Structure determination
Method | negative staining |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 7.4 |
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Staining | Type: NEGATIVE / Material: uranyl formate |
-Electron microscopy
Microscope | FEI TECNAI F20 |
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Image recording | Film or detector model: TVIPS TEMCAM-F416 (4k x 4k) / Average electron dose: 50.0 e/Å2 |
Electron beam | Acceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: -2.0 µm / Nominal defocus min: -1.0 µm |
Experimental equipment | Model: Tecnai F20 / Image courtesy: FEI Company |
-Image processing
Startup model | Type of model: PDB ENTRY PDB model - PDB ID: |
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Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 20.0 Å / Resolution method: FSC 0.5 CUT-OFF / Number images used: 989 |
Initial angle assignment | Type: ANGULAR RECONSTITUTION |
Final angle assignment | Type: ANGULAR RECONSTITUTION |