EMDB-26821: A. baumannii ribosome-Streptothricin-D complex: 70S with E-site tRNA

Basic information

Entry

Database: EMDB / ID: EMD-26821

• Title: A. baumannii ribosome-Streptothricin-D complex: 70S with E-site tRNA
• Map data: A. baumannii 70S Ribosome-SD complex with E-site tRNA

Sample

• Complex: SD-70S Ribosome Complex with E-site tRNA
  • Protein or peptide: x 48 types
  • RNA: x 5 types
• Ligand: x 4 types

• Keywords: Streptothricin / Nourseothricin / Antibiotic / Ribosome / Ribosome-RNA complex

Function / homology

Function:

ribosomal small subunit biogenesis / small ribosomal subunit rRNA binding / large ribosomal subunit / ribosomal small subunit assembly / small ribosomal subunit / 5S rRNA binding / large ribosomal subunit rRNA binding / transferase activity / cytosolic small ribosomal subunit / ribosomal large subunit assembly / cytoplasmic translation / cytosolic large ribosomal subunit / tRNA binding / negative regulation of translation / rRNA binding / ribosome / structural constituent of ribosome / ribonucleoprotein complex / translation / mRNA binding / RNA binding / cytosol / cytoplasm

Domain/homology:

Ribosomal protein S21, conserved site / Ribosomal protein S21 signature. / Ribosomal protein L25, short-form / Ribosomal protein S14, bacterial/plastid / Ribosomal protein S21 superfamily / Ribosomal protein S21 / Ribosomal protein S16, conserved site / Ribosomal protein S16 signature. / Ribosomal protein S21 / : / Ribosomal protein L21, conserved site / Ribosomal protein L21 signature. / : / Ribosomal protein L6, conserved site / Ribosomal protein L6 signature 1. / Ribosomal protein L16, conserved site / Ribosomal protein L16 signature 2. / Ribosomal protein L9 signature. / Ribosomal protein L9, bacteria/chloroplast / Ribosomal protein L9, C-terminal / Ribosomal protein L9, C-terminal domain / Ribosomal protein L9, C-terminal domain superfamily / Ribosomal protein L17 signature. / Ribosomal L25p family / Ribosomal protein L25 / Ribosomal protein L36 signature. / Ribosomal protein L28/L24 superfamily / Ribosomal protein L25/Gln-tRNA synthetase, N-terminal / Ribosomal protein L25/Gln-tRNA synthetase, anti-codon-binding domain superfamily / Ribosomal protein L32p, bacterial type / Ribosomal protein L9, N-terminal domain superfamily / Ribosomal protein L9 / Ribosomal protein L9, N-terminal / Ribosomal protein L9, N-terminal domain / : / : / Ribosomal protein L28 / Ribosomal protein L35, conserved site / Ribosomal protein L35 signature. / Ribosomal protein L33, conserved site / Ribosomal protein L33 signature. / Ribosomal protein L35, non-mitochondrial / Ribosomal protein L5, bacterial-type / Ribosomal protein L18, bacterial-type / Ribosomal protein L6, bacterial-type / Ribosomal protein L9/RNase H1, N-terminal / Ribosomal protein L19, conserved site / Ribosomal protein L19 signature. / Ribosomal protein S3, bacterial-type / Ribosomal protein S6, conserved site / Ribosomal protein S6 signature. / Ribosomal protein S19, bacterial-type / Ribosomal protein S13, bacterial-type / Ribosomal protein L36 / Ribosomal protein L36 superfamily / Ribosomal protein L36 / Ribosomal protein S7, bacterial/organellar-type / Ribosomal protein S11, bacterial-type / Ribosomal protein S20 / Ribosomal protein S20 superfamily / Ribosomal protein S20 / Ribosomal protein L20 signature. / Ribosomal protein S9, bacterial/plastid / Ribosomal protein L27, conserved site / Ribosomal protein L27 signature. / Ribosomal protein S4, bacterial-type / 30S ribosomal protein S17 / Ribosomal protein S5, bacterial-type / Ribosomal protein L14P, bacterial-type / Ribosomal protein S6, plastid/chloroplast / Ribosomal protein L22, bacterial/chloroplast-type / Ribosomal protein L2, bacterial/organellar-type / Ribosomal protein S2, bacteria/mitochondria/plastid / Ribosomal protein L35 / Ribosomal protein L35 superfamily / Ribosomal protein L35 / Ribosomal protein L34, conserved site / Ribosomal L28 family / Ribosomal protein L34 signature. / Ribosomal protein L33 / Ribosomal protein L33 / Ribosomal protein L28/L24 / Ribosomal protein L18 / Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast / Ribosomal protein L33 superfamily / Ribosomal protein L30, bacterial-type / Ribosomal protein S18, conserved site / Ribosomal protein S18 signature. / Ribosomal protein L16 / Ribosomal protein S16 / Ribosomal protein S16 domain superfamily / Ribosomal protein S16 / : / L28p-like / Ribosomal protein L20 / Ribosomal protein S15, bacterial-type / Ribosomal protein L20 / Ribosomal protein L20, C-terminal / Ribosomal protein L21 / Ribosomal protein L27

Component:

Large ribosomal subunit protein uL22 / 30S ribosomal protein S10 / Small ribosomal subunit protein bS21 / Large ribosomal subunit protein bL34 / Small ribosomal subunit protein bS16 / Small ribosomal subunit protein bS18 / Small ribosomal subunit protein uS11 / Large ribosomal subunit protein bL33 / Small ribosomal subunit protein uS15 / Small ribosomal subunit protein bS20 / Large ribosomal subunit protein bL35 / Large ribosomal subunit protein bL27 / Large ribosomal subunit protein bL21 / Large ribosomal subunit protein bL32 / Large ribosomal subunit protein bL25 / Small ribosomal subunit protein uS12 / Small ribosomal subunit protein uS7 / Large ribosomal subunit protein uL13 / Large ribosomal subunit protein bL17 / Small ribosomal subunit protein uS4 / Small ribosomal subunit protein uS13 / Large ribosomal subunit protein bL36 / Large ribosomal subunit protein uL15 / Large ribosomal subunit protein uL30 / Small ribosomal subunit protein uS5 / Large ribosomal subunit protein uL18 / Large ribosomal subunit protein uL6 / Small ribosomal subunit protein uS8 / Small ribosomal subunit protein uS14 / Large ribosomal subunit protein uL5 / Large ribosomal subunit protein uL24 / Small ribosomal subunit protein uS17 / Large ribosomal subunit protein uL29 / Large ribosomal subunit protein uL16 / Small ribosomal subunit protein uS19 / Large ribosomal subunit protein uL2 / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein bL19 / Small ribosomal subunit protein bS6 / Large ribosomal subunit protein bL9 / Large ribosomal subunit protein uL14 / Large ribosomal subunit protein bL28 / Small ribosomal subunit protein uS3 / Small ribosomal subunit protein uS9 / Small ribosomal subunit protein uS2 / Large ribosomal subunit protein bL20

• Biological species: Acinetobacter baumannii AB0057 (bacteria)
• Method: single particle reconstruction / cryo EM / Resolution: 2.21 Å
• Authors: Morgan CE / Yu EW

Funding support

United States, 1 items

Organization	Grant number	Country
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)		United States

• Citation: Journal: PLoS Biol / Year: 2023; Title: Streptothricin F is a bactericidal antibiotic effective against highly drug-resistant gram-negative bacteria that interacts with the 30S subunit of the 70S ribosome.; Authors: Christopher E Morgan / Yoon-Suk Kang / Alex B Green / Kenneth P Smith / Matthew G Dowgiallo / Brandon C Miller / Lucius Chiaraviglio / Katherine A Truelson / Katelyn E Zulauf / Shade Rodriguez / Anthony D Kang / Roman Manetsch / Edward W Yu / James E Kirby / ; Abstract: The streptothricin natural product mixture (also known as nourseothricin) was discovered in the early 1940s, generating intense initial interest because of excellent gram-negative activity. Here, we establish the activity spectrum of nourseothricin and its main components, streptothricin F (S-F, 1 lysine) and streptothricin D (S-D, 3 lysines), purified to homogeneity, against highly drug-resistant, carbapenem-resistant Enterobacterales (CRE) and Acinetobacter baumannii. For CRE, the MIC50 and MIC90 for S-F and S-D were 2 and 4 μM, and 0.25 and 0.5 μM, respectively. S-F and nourseothricin showed rapid, bactericidal activity. S-F and S-D both showed approximately 40-fold greater selectivity for prokaryotic than eukaryotic ribosomes in in vitro translation assays. In vivo, delayed renal toxicity occurred at >10-fold higher doses of S-F compared with S-D. Substantial treatment effect of S-F in the murine thigh model was observed against the otherwise pandrug-resistant, NDM-1-expressing Klebsiella pneumoniae Nevada strain with minimal or no toxicity. Cryo-EM characterization of S-F bound to the A. baumannii 70S ribosome defines extensive hydrogen bonding of the S-F steptolidine moiety, as a guanine mimetic, to the 16S rRNA C1054 nucleobase (Escherichia coli numbering) in helix 34, and the carbamoylated gulosamine moiety of S-F with A1196, explaining the high-level resistance conferred by corresponding mutations at the residues identified in single rrn operon E. coli. Structural analysis suggests that S-F probes the A-decoding site, which potentially may account for its miscoding activity. Based on unique and promising activity, we suggest that the streptothricin scaffold deserves further preclinical exploration as a potential therapeutic for drug-resistant, gram-negative pathogens.

History

Deposition	May 2, 2022	-
Header (metadata) release	Apr 19, 2023	-
Map release	Apr 19, 2023	-
Update	Jun 12, 2024	-
Current status	Jun 12, 2024	Processing site: RCSB / Status: Released

Map

• File: Download / File: emd_26821.map.gz / Format: CCP4 / Size: 1000 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Annotation: A. baumannii 70S Ribosome-SD complex with E-site tRNA
• Voxel size: X=Y=Z: 0.72 Å

Density

Contour Level	By AUTHOR: 0.1
Minimum - Maximum	-0.039884165 - 0.9751709
Average (Standard dev.)	0.0029669704 (±0.023710506)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 640 640 640 Spacing 640 640 640
Cell	A=B=C: 460.80002 Å α=β=γ: 90.0 °

Supplemental data

Sample components

Entire : SD-70S Ribosome Complex with E-site tRNA

• Entire: Name: SD-70S Ribosome Complex with E-site tRNA

Components

• Complex: SD-70S Ribosome Complex with E-site tRNA
  • Protein or peptide: 50S ribosomal protein L33
  • Protein or peptide: 50S ribosomal protein L34
  • Protein or peptide: 50S ribosomal protein L35
  • Protein or peptide: 50S ribosomal protein L36
  • RNA: 23s ribosomal RNA
  • RNA: 5s ribosomal RNA
  • Protein or peptide: 50S ribosomal protein L2
  • Protein or peptide: 50S ribosomal protein L3
  • Protein or peptide: 50S ribosomal protein L4
  • Protein or peptide: 50S ribosomal protein L5
  • Protein or peptide: 50S ribosomal protein L6
  • Protein or peptide: 50S ribosomal protein L9
  • Protein or peptide: 50S ribosomal protein L13
  • Protein or peptide: 50S ribosomal protein L14
  • Protein or peptide: 50S ribosomal protein L15
  • Protein or peptide: 50S ribosomal protein L16
  • Protein or peptide: 50S ribosomal protein L17
  • Protein or peptide: 50S ribosomal protein L18
  • Protein or peptide: 50S ribosomal protein L19
  • Protein or peptide: 50S ribosomal protein L20
  • Protein or peptide: 50S ribosomal protein L21
  • Protein or peptide: 50S ribosomal protein L22
  • Protein or peptide: 50S ribosomal protein L23
  • Protein or peptide: 50S ribosomal protein L24
  • Protein or peptide: 50S ribosomal protein L25
  • Protein or peptide: 50S ribosomal protein L27
  • Protein or peptide: 50S ribosomal protein L28
  • Protein or peptide: 50S ribosomal protein L29
  • Protein or peptide: 50S ribosomal protein L30
  • Protein or peptide: 50S ribosomal protein L32
  • RNA: 16s Ribosomal RNA
  • Protein or peptide: 30S ribosomal protein S2
  • Protein or peptide: 30S ribosomal protein S3
  • Protein or peptide: 30S ribosomal protein S4
  • Protein or peptide: 30S ribosomal protein S5
  • Protein or peptide: 30S ribosomal protein S6
  • Protein or peptide: 30S ribosomal protein S7
  • Protein or peptide: 30S ribosomal protein S8
  • Protein or peptide: 30S ribosomal protein S9
  • Protein or peptide: 30S ribosomal protein S10
  • Protein or peptide: 30S ribosomal protein S11
  • Protein or peptide: 30S ribosomal protein S12
  • Protein or peptide: 30S ribosomal protein S13
  • Protein or peptide: 30S ribosomal protein S14
  • Protein or peptide: 30S ribosomal protein S15
  • Protein or peptide: 30S ribosomal protein S16
  • Protein or peptide: 30S ribosomal protein S17
  • Protein or peptide: 30S ribosomal protein S18
  • Protein or peptide: 30S ribosomal protein S19
  • Protein or peptide: 30S ribosomal protein S20
  • Protein or peptide: 30S ribosomal protein S21
  • RNA: tRNA-met
  • RNA: mRNA
• Ligand: ZINC ION
• Ligand: Streptothricin D
• Ligand: MAGNESIUM ION
• Ligand: water

Supramolecule #1: SD-70S Ribosome Complex with E-site tRNA

• Supramolecule: Name: SD-70S Ribosome Complex with E-site tRNA / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#53
• Source (natural): Organism: Acinetobacter baumannii AB0057 (bacteria)

Macromolecule #1: 50S ribosomal protein L33

• Macromolecule: Name: 50S ribosomal protein L33 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Acinetobacter baumannii AB0057 (bacteria) / Strain: AB0057
• Molecular weight: Theoretical: 6.10531 KDa

Sequence

String:

MRDKIRLVSS AGTGYFYTTT KNKRTMPEKM EIKKFDPKIR QHVIFKEAKI K

UniProtKB: Large ribosomal subunit protein bL33

Macromolecule #2: 50S ribosomal protein L34

• Macromolecule: Name: 50S ribosomal protein L34 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Acinetobacter baumannii AB0057 (bacteria)
• Molecular weight: Theoretical: 5.192247 KDa

Sequence

String:

MKRTFQPSEL KRKRVHGFRA RMATKAGRQV LARRRAKGRH SLTV

UniProtKB: Large ribosomal subunit protein bL34

Macromolecule #3: 50S ribosomal protein L35

• Macromolecule: Name: 50S ribosomal protein L35 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Acinetobacter baumannii AB0057 (bacteria) / Strain: AB0057
• Molecular weight: Theoretical: 7.424098 KDa

Sequence

String:

MAKLKTRRGA AKRFKATANG FKRKQAFKRH ILTKKSAKRI RQLRGCVMVH VSDVASVRRM CPYI

UniProtKB: Large ribosomal subunit protein bL35

Macromolecule #4: 50S ribosomal protein L36

• Macromolecule: Name: 50S ribosomal protein L36 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Acinetobacter baumannii AB0057 (bacteria) / Strain: AB0057
• Molecular weight: Theoretical: 4.276201 KDa

Sequence

String:

MKVQASVKKI CGSCKVIRRN GVIRVICSAE PRHKQRQG

UniProtKB: Large ribosomal subunit protein bL36

Macromolecule #7: 50S ribosomal protein L2

• Macromolecule: Name: 50S ribosomal protein L2 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Acinetobacter baumannii AB0057 (bacteria) / Strain: AB0057
• Molecular weight: Theoretical: 30.346115 KDa

Sequence

String:

MPIQKCKPTS PGRRFVEKVV HDHLHKGAPY APLVEAKKRT GGRNNNGHIT TRHVGGGHKQ HYRIVDFKRN KDGVPAVVER IEYDPNRTA HIALLKYADG ERRYIIAPKG LRAGDKVQSG NDAPIRPGNC LPLRNMPIGS TLHNVELKIG KGAQLARSAG A SVQLLGRD GSYAIIRLRS GEMRKVHVEC RAVIGEVSNQ ENNLRSLGKA GAARWRGVRP TVRGMAMNPI DHPHGGGEGR NK GIQPVSP WGQKAKGYKT RTNKRTTKMI IRDRRVK

UniProtKB: Large ribosomal subunit protein uL2

Macromolecule #8: 50S ribosomal protein L3

• Macromolecule: Name: 50S ribosomal protein L3 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Acinetobacter baumannii AB0057 (bacteria) / Strain: AB0057
• Molecular weight: Theoretical: 22.512639 KDa

Sequence

String:

MAIGLVGRKC GMTRIFTDAG VSVPVTVIEV DPNRITQIKT LETDGYQAVQ VTTGERRESR VTNAQKGHFA KAGVAAGRLV KEFRVTEAE LEGREVGGTI GVDLFTVGQI VDVTGQSKGK GFQGGVKRWN FRTQDATHGN SVSHRVLGST GQNQTPGRVF K GKKMAGHL GDERVTVQGL EIVSVDTERS VLVVKGAIPG ATGGDVIVRP TIKA

UniProtKB: Large ribosomal subunit protein uL3

Macromolecule #9: 50S ribosomal protein L4

• Macromolecule: Name: 50S ribosomal protein L4 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Acinetobacter baumannii AB0057 (bacteria) / Strain: AB0057
• Molecular weight: Theoretical: 21.583904 KDa

Sequence

String:

MNLKTVSGSA VELSEVAFGR EFNEALVHQV VTAYLAGGRQ GTRAHKSRAD VSGGGKKPFR QKGTGRARAG SIRSPIWVGG GKTFAARPQ DWSQKVNRKM YRGAMQCILA ELVRQDRLVL VEEFAVAAPK TKELLAKLND LNAARALIVT DAVDENLYLA A RNLPHVDV VDATAIDPVS LIAFDKVVMS VAAAKKIEVE LG

UniProtKB: Large ribosomal subunit protein uL4

Macromolecule #10: 50S ribosomal protein L5

• Macromolecule: Name: 50S ribosomal protein L5 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Acinetobacter baumannii AB0057 (bacteria) / Strain: AB0057
• Molecular weight: Theoretical: 20.051471 KDa

Sequence

String:

MARLKARYND ELKAKLQEEL SIKNVMEIPR ITKITLNMGV GAAATDKKLL DGAVADMQLI AGQKPVVTLA RKSIAGFKIR DGWPIGCKV TLRGDQMYEF LDRLISIAIP RIRDFRGFSA KSFDGRGNYS MGLKEQIVFP EIDFDKIDRI RGMDITITTT A RTDDEGRA LMRAFGFPFK

UniProtKB: Large ribosomal subunit protein uL5

Macromolecule #11: 50S ribosomal protein L6

• Macromolecule: Name: 50S ribosomal protein L6 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Acinetobacter baumannii AB0057 (bacteria) / Strain: AB0057
• Molecular weight: Theoretical: 19.126047 KDa

Sequence

String:

MSRVAKAPVT VPNGVTVTQN GRQVEVKGSK GTLSFNLHAL VELKQEEGKL QLAPAKESKD AWMQAGTARA VLNNLVKGVS EGFERKLQL VGVGYKAAVK GTVVNLNLGY SHPIDYALPE GVTAETPTAT EIILKSANKQ LLGQVAAEIR AYRSPEPYKG K GVRYSDEV ILRKEAKKK

UniProtKB: Large ribosomal subunit protein uL6

Macromolecule #12: 50S ribosomal protein L9

• Macromolecule: Name: 50S ribosomal protein L9 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Acinetobacter baumannii AB0057 (bacteria) / Strain: AB0057
• Molecular weight: Theoretical: 15.800955 KDa

Sequence

String:

MDVILLQRIK NLGKLGDKVS VKAGYGRNFL IPQGKAVAAT EANTAAFEAR RAELEKQEAE VLAAAQARAE QLNEVNIVIT AKAGDEGKL FGSIGTRDIA DALTNAGLTV DRAEVRLPNG ALRHTGEFNI AIQLHHDVVA EVLVTIVSE

UniProtKB: Large ribosomal subunit protein bL9

Macromolecule #13: 50S ribosomal protein L13

• Macromolecule: Name: 50S ribosomal protein L13 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Acinetobacter baumannii AB0057 (bacteria) / Strain: AB0057
• Molecular weight: Theoretical: 15.981466 KDa

Sequence

String:

MKTLSAKPAE VQHDWFVVDA TGKTLGRLAT EIARRLRGKH KTSYTPHVDT GDYIIVINAE QVQVTGNKAL DKKYYRHTEF PGGLKETNF EKLVAHKPEE IFERAVKGML PKGPLGYAMI KKMKVYAGSE HPHAAQQPQV LDI

UniProtKB: Large ribosomal subunit protein uL13

Macromolecule #14: 50S ribosomal protein L14

• Macromolecule: Name: 50S ribosomal protein L14 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Acinetobacter baumannii AB0057 (bacteria)
• Molecular weight: Theoretical: 13.523863 KDa

Sequence

String:

MIQTETMLDV ADNSGARRVQ CIKVLGGSHR RYASVGDIIK VTVKEAIPRA RVKKGDVMNA VVVRTKFGIR RPDGSVIRFD DNAAVILNN NKAPIATRIF GPVTRELRTE QFMKIISLAP EVL

UniProtKB: Large ribosomal subunit protein uL14

Macromolecule #15: 50S ribosomal protein L15

• Macromolecule: Name: 50S ribosomal protein L15 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Acinetobacter baumannii AB0057 (bacteria) / Strain: AB0057
• Molecular weight: Theoretical: 15.509915 KDa

Sequence

String:

MTLRLNELAP AEGAKREHRR LGRGIGSGVG KTGGRGIKGQ KSRKSGGVRP GFEGGQTAIY RRLPKFGFTS QIALKTAEVR LSELSKVEG DIVSLETLKA ANVVRRDQIR ARIVLSGEIT RAFTVQGVAL TKGAKAAIEA AGGKVEE

UniProtKB: Large ribosomal subunit protein uL15

Macromolecule #16: 50S ribosomal protein L16

• Macromolecule: Name: 50S ribosomal protein L16 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Acinetobacter baumannii AB0057 (bacteria) / Strain: AB0057
• Molecular weight: Theoretical: 15.498312 KDa

Sequence

String:

MLQPKRTKFR KVHKGRNTGL AHRGSTVSFG SIAIKATERG RMTARQIEAA RRTISRRIKR GGKIFIRVFP DKPITEKPLE VRMGNGKGN VEYWVCEIKP GKILYEIEGV NEDLAREAFA LAAAKLPFKT TIVTRTVM

UniProtKB: Large ribosomal subunit protein uL16

Macromolecule #17: 50S ribosomal protein L17

• Macromolecule: Name: 50S ribosomal protein L17 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Acinetobacter baumannii AB0057 (bacteria) / Strain: AB0057
• Molecular weight: Theoretical: 14.022215 KDa

Sequence

String:

MRHRNSGVKL GRTSSHRKAM FENLANSLFE HELIKTTLPK AKELRRVAEP LITLAKNDTV ANRRLAFART RNAATVGKLF TVLGPRYKE RNGGYLRVLK AGFRAGDAAP MAYVELVDRE VNTSAE

UniProtKB: Large ribosomal subunit protein bL17

Macromolecule #18: 50S ribosomal protein L18

• Macromolecule: Name: 50S ribosomal protein L18 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Acinetobacter baumannii AB0057 (bacteria) / Strain: AB0057
• Molecular weight: Theoretical: 12.443341 KDa

Sequence

String:

MNEKKQSRLR RAKSTRLHIR ALGATRLCVN RTPRHIYAQV ISADGGKVLA QASTLDASLR SGTTGNIEAA TKVGALIAER AKAAGVTKV AFDRSGFKYH GRIKALADAA REGGLEF

UniProtKB: Large ribosomal subunit protein uL18

Macromolecule #19: 50S ribosomal protein L19

• Macromolecule: Name: 50S ribosomal protein L19 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Acinetobacter baumannii AB0057 (bacteria) / Strain: AB0057
• Molecular weight: Theoretical: 13.619865 KDa

Sequence

String:

MSGKHPLVQA IENSQLKTDL PEFAPGDTVV VQVKVKEGDR ERLQAFEGVV IAKKNRGLNS AFTVRKISSG VGVERVFQTH SPVVAKIEV KRRGDVRRAK LYYLRDLSGK AARIREKLPA RKA

UniProtKB: Large ribosomal subunit protein bL19

Macromolecule #20: 50S ribosomal protein L20

• Macromolecule: Name: 50S ribosomal protein L20 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Acinetobacter baumannii AB0057 (bacteria)
• Molecular weight: Theoretical: 13.468946 KDa

Sequence

String:

MARVKRGVVA HRRHKKILAR AKGYYGARSR VYRVAFQAVI KAGQYAYRDR RQKKRQFRAL WIARINAGAR QNGLSYSRMI DGLKKAQVI IDRRVLADIA MHDAVAFAAL AEKAKGALAA

UniProtKB: Large ribosomal subunit protein bL20

Macromolecule #21: 50S ribosomal protein L21

• Macromolecule: Name: 50S ribosomal protein L21 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Acinetobacter baumannii AB0057 (bacteria) / Strain: AB0057
• Molecular weight: Theoretical: 11.496364 KDa

Sequence

String:

MYAVIQSGGK QHRVVEGETL KVELLKAESG ATITFDDVLM VVNGDNIQIG APVVAGAKVT AEVIGHGRHD KIRIIKMRRR KHYRKQQGH RQWFTELKIT GISG

UniProtKB: Large ribosomal subunit protein bL21

Macromolecule #22: 50S ribosomal protein L22

• Macromolecule: Name: 50S ribosomal protein L22 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Acinetobacter baumannii AB0057 (bacteria)
• Molecular weight: Theoretical: 11.828931 KDa

Sequence

String:

MEVTAKLRGA AISAQKARLV ADLIRGKSVA HALNILNFSN KKAAVLVKKA LESAIANAEH NNSLDVDDLK VSTIYVDEGM SLKRIMPRA KGRADRITKR TCHITVKVGV

UniProtKB: Large ribosomal subunit protein uL22

Macromolecule #23: 50S ribosomal protein L23

• Macromolecule: Name: 50S ribosomal protein L23 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Acinetobacter baumannii AB0057 (bacteria) / Strain: AB0057
• Molecular weight: Theoretical: 11.607392 KDa

Sequence

String:

MNNERIYQVL KGPVFSEKAQ VLGDTAGVQV FKVDINATKL EIKKAVEKLF GVEVVKVNTT ITKGKTKRFG RTLGRRSDVK KAYVTLKAG QDVEMADLGD TAESAAE

UniProtKB: Large ribosomal subunit protein uL23

Macromolecule #24: 50S ribosomal protein L24

• Macromolecule: Name: 50S ribosomal protein L24 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Acinetobacter baumannii AB0057 (bacteria) / Strain: AB0057
• Molecular weight: Theoretical: 11.187929 KDa

Sequence

String:

MAKIKKGDQV IVIAGKEKGK QGTVLSVSED RVKVEGLNLV KKHQKPNRVT GAEGGIVTQE ASLHISNVAI LNATTQKADR VGYQVIDGV KTRVYKSTGE SVAVAK

UniProtKB: Large ribosomal subunit protein uL24

Macromolecule #25: 50S ribosomal protein L25

• Macromolecule: Name: 50S ribosomal protein L25 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Acinetobacter baumannii AB0057 (bacteria) / Strain: AB0057
• Molecular weight: Theoretical: 10.942593 KDa

Sequence

String:

MANFVLNAQA RAEDKQGKGA SRRLRRESLV PAIIYGGNAE PVAVTLELRE LVKALESNVF FEEVVEIKVG DKVENVKIQA LQRHPAKNT PMHADFKRA

UniProtKB: Large ribosomal subunit protein bL25

Macromolecule #26: 50S ribosomal protein L27

• Macromolecule: Name: 50S ribosomal protein L27 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Acinetobacter baumannii AB0057 (bacteria) / Strain: AB0057
• Molecular weight: Theoretical: 9.072387 KDa

Sequence

String:

MATKKAGGST KNGRDSNPKM LGVKVYGGQT VTAGNIIVRQ RGTEFHAGAN VGMGRDHTLF ATADGVVKFE VKGQFGRRYV KVETV

UniProtKB: Large ribosomal subunit protein bL27

Macromolecule #27: 50S ribosomal protein L28

• Macromolecule: Name: 50S ribosomal protein L28 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Acinetobacter baumannii AB0057 (bacteria)
• Molecular weight: Theoretical: 9.125756 KDa

Sequence

String:

MSKVCQVTGK RPVVGNNVSH ANNKTKRRFE PNLHHHRFWL ESEKRFVRLR LTTKGMRIID KLGIEKVVAD LRAQGQKI

UniProtKB: Large ribosomal subunit protein bL28

Macromolecule #28: 50S ribosomal protein L29

• Macromolecule: Name: 50S ribosomal protein L29 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Acinetobacter baumannii AB0057 (bacteria) / Strain: AB0057
• Molecular weight: Theoretical: 7.449678 KDa

Sequence

String:

MKTKDLREKS VEELKALLDE QQLNQFRLRM AKATGQLGKS HEVQVARKTI ARIKTLLTEK QGNGQ

UniProtKB: Large ribosomal subunit protein uL29

Macromolecule #29: 50S ribosomal protein L30

• Macromolecule: Name: 50S ribosomal protein L30 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Acinetobacter baumannii AB0057 (bacteria) / Strain: AB0057
• Molecular weight: Theoretical: 6.654788 KDa

Sequence

String:

MKTIKVTQTK SSSHRLKNHK LCLQGLGLRR IGHTVEVQDT PSNRGMINKV YYMVSVEE

UniProtKB: Large ribosomal subunit protein uL30

Macromolecule #30: 50S ribosomal protein L32

• Macromolecule: Name: 50S ribosomal protein L32 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Acinetobacter baumannii AB0057 (bacteria) / Strain: AB0057
• Molecular weight: Theoretical: 7.096888 KDa

Sequence

String:

MAVQQNRKSR SRRDMRRSHD ALTENALTVD QATGETHRRH HVTKDGFYRG RQLFAKAADA E

UniProtKB: Large ribosomal subunit protein bL32

Macromolecule #32: 30S ribosomal protein S2

• Macromolecule: Name: 30S ribosomal protein S2 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Acinetobacter baumannii AB0057 (bacteria)
• Molecular weight: Theoretical: 27.680357 KDa

Sequence

String:

MADYNVSMRD LLQAGAHFGH QTRFWNPKMR QYIFGARNKI HIINLEHTVP ALNDALNFAN QLASKKNKVL FVGTKRAASN IIREQAQRA GQPYVDHRWL GGMLTNWKTL RQSINRLKDL QTQSQDGTFA KLTKREALER TREMEKLERS LGGVKNMGGL P DALFVIDV DHEAIAIKEA KNLGIPVIGI VDTNSNPDNV DYVIPGNDDA IRAVTLYASA MADAILAGKE YAQSQANAQA KG DDAAKDA SEA

UniProtKB: Small ribosomal subunit protein uS2

Macromolecule #33: 30S ribosomal protein S3

• Macromolecule: Name: 30S ribosomal protein S3 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Acinetobacter baumannii AB0057 (bacteria)
• Molecular weight: Theoretical: 27.972461 KDa

Sequence

String:

MGQKVHPIGI RLGVVKRHNA NWYANPKQYA EYLLKDLQVR EFLTKKLKNA MVSNILIERP SGAAKVTIST ARPGIVIGKK GEDIEKLQR ELTNIMGVPA QVSINEIDRP DLDARLVAEA IASQLEKRVM FRRAMKRAVQ NTMRAGAKGI KVEVSGRLGG A EIARTEWY REGRVPLHTL RADIDYATMR AETTYGTIGV KVWIFRGEIL GGMKQVMNPA PAEERPAKRG RGRGEGQERR GR RGDRAAD KGE

UniProtKB: Small ribosomal subunit protein uS3

Macromolecule #34: 30S ribosomal protein S4

• Macromolecule: Name: 30S ribosomal protein S4 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Acinetobacter baumannii AB0057 (bacteria) / Strain: AB0057
• Molecular weight: Theoretical: 23.311818 KDa

Sequence

String:

MARYIGPKCK LSRREGTDLQ LKSGVKPFDV KTKKANKAPG QHGQARGGKQ SEYSLQLREK QKVRRIYGVL ERQFSNYYKE AARVKGATG ENLLKLLESR LDNVVYRMGF GSTRAEARQL VSHRSITLNG RRVNIASIQV KAGDVIAVHE GAKQQLRIKN A IELAAQRG IPAWIEVDHS KLEGTFKAAP DRSDLPAEIN ESLIVELYSK

UniProtKB: Small ribosomal subunit protein uS4

Macromolecule #35: 30S ribosomal protein S5

• Macromolecule: Name: 30S ribosomal protein S5 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Acinetobacter baumannii AB0057 (bacteria) / Strain: AB0057
• Molecular weight: Theoretical: 17.181766 KDa

Sequence

String:

MAKVEQNEGL VEKLVAVDRV AKVVKGGRIF SFTALTVVGD GNGRVGFGRG KAREVPAAIS KALEAARRNM ITVDLAGTTL QHPVNARHG ASRVYMQPAS EGTGVIAGGA MRAVLEAAGV HNVLAKCYGS TNAANVVNAT FKGLRDMTSP EKVAAKRGKS V EEIQG

UniProtKB: Small ribosomal subunit protein uS5

Macromolecule #36: 30S ribosomal protein S6

• Macromolecule: Name: 30S ribosomal protein S6 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Acinetobacter baumannii AB0057 (bacteria) / Strain: AB0057
• Molecular weight: Theoretical: 14.986952 KDa

Sequence

String:

MRHYEIVLLV HPDQSDQVVG MVERYISQIK EADGQIHRLE DWGRRQLAYP INKIHKAHYI LMNVECGQST LDELEELFRY NDAIIRNLI IRREHAITEE SLLAKSAEEK RARKAQREEA QQVAQEAE

UniProtKB: Small ribosomal subunit protein bS6

Macromolecule #37: 30S ribosomal protein S7

• Macromolecule: Name: 30S ribosomal protein S7 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Acinetobacter baumannii AB0057 (bacteria) / Strain: AB0057
• Molecular weight: Theoretical: 17.733699 KDa

Sequence

String:

MPRRRVVAAR EILPDPKFSS QTIAKFMNHV MQDGKKSIAE SIVYGALERV QEKNKVDPVE FFETTLEKVR PMVEVKARRV GGATYQVPM EVRPSRRTAL AMRWLVDAAA KRSEKTMALR LAGELLDAAE GKGAAIKKRE DVHRMAEANK AFSHYRF

UniProtKB: Small ribosomal subunit protein uS7

Macromolecule #38: 30S ribosomal protein S8

• Macromolecule: Name: 30S ribosomal protein S8 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Acinetobacter baumannii AB0057 (bacteria) / Strain: AB0057
• Molecular weight: Theoretical: 14.250667 KDa

Sequence

String:

MSMQDTVADM LTRVRNAQMA KKQTVSMPSS KLKVAIANVL QQEGYISNVE VAQEETKSTL TITLKYFEGK PVIEMVKRVS RPGLRQYRG KDKLPSVKQG LGIAIVSTSK GIMTDRAARA AGIGGEVIAF VS

UniProtKB: Small ribosomal subunit protein uS8

Macromolecule #39: 30S ribosomal protein S9

• Macromolecule: Name: 30S ribosomal protein S9 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Acinetobacter baumannii AB0057 (bacteria)
• Molecular weight: Theoretical: 14.28761 KDa

Sequence

String:

MATNYGTGRR KTATARVFLS AGTGKLVINN RTLEQYFGRE TARMVVRQPL ELLEATEKYD LYITVKGGGI GGQAGAIRHG ITRALIAAD ETLKPVLRQA GFVTRDAREV ERKKLGLRKA RKRPQFSKR

UniProtKB: Small ribosomal subunit protein uS9

Macromolecule #40: 30S ribosomal protein S10

• Macromolecule: Name: 30S ribosomal protein S10 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Acinetobacter baumannii AB0057 (bacteria)
• Molecular weight: Theoretical: 11.718531 KDa

Sequence

String:

MSNQRIRIRL KSFDHRLIDQ SAQEIVETAK RTGAQVCGPI PMPTRIERFN VLTSPHVNKD ARDQYEIRTY KRLIDIVQPT DKTVDALMK LDLAAGVDVQ IALG

UniProtKB: 30S ribosomal protein S10

Macromolecule #41: 30S ribosomal protein S11

• Macromolecule: Name: 30S ribosomal protein S11 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Acinetobacter baumannii AB0057 (bacteria)
• Molecular weight: Theoretical: 13.558512 KDa

Sequence

String:

MAKDTRTRKK VTRTVSEGVA HIHASFNNTI VTITDRQGNA LAWATSGGQG FRGSRKSTPF AAQVAAEVAG KAALDYGLKN LDVLVKGPG PGRESAVRAL GAVGYKINSI TDVTPIPHNG CRPPKKRRV

UniProtKB: Small ribosomal subunit protein uS11

Macromolecule #42: 30S ribosomal protein S12

• Macromolecule: Name: 30S ribosomal protein S12 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Acinetobacter baumannii AB0057 (bacteria) / Strain: AB0057
• Molecular weight: Theoretical: 13.797134 KDa

Sequence

String:

MATTNQLIRK GRTTLVEKSK VPALKACPQR RGVCTRVYTT TPKKPNSAMR KVCRVRLTSG FEVSSYIGGE GHNLQEHSVV LIRGGRVKD LPGVRYHTVR GSLDCAGVKD RNQSRSKYGA KRPKK

UniProtKB: Small ribosomal subunit protein uS12

Macromolecule #43: 30S ribosomal protein S13

• Macromolecule: Name: 30S ribosomal protein S13 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Acinetobacter baumannii AB0057 (bacteria) / Strain: AB0057
• Molecular weight: Theoretical: 13.295635 KDa

Sequence

String:

MARIAGVNIP DNKHAVISLT YIFGIGRHTA KNILAAVGIT ETTKIRELDD AQLDAIRAEV AKVPTEGDLR REISMNIKRL MDLGCYRGL RHRRSLPVRG QRTKTNARTR KGPRKPIKK

UniProtKB: Small ribosomal subunit protein uS13

Macromolecule #44: 30S ribosomal protein S14

• Macromolecule: Name: 30S ribosomal protein S14 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Acinetobacter baumannii AB0057 (bacteria) / Strain: AB0057
• Molecular weight: Theoretical: 11.438427 KDa

Sequence

String:

MAKKGMINRE LKREKTVAKY AAKRAELKAT IANVNASDEE RFEAMLKLQA LPRNASPVRL RNRCGLTGRP HGYFRKFGLS RNKLRDTVM QGDVPGVVKA SW

UniProtKB: Small ribosomal subunit protein uS14

Macromolecule #45: 30S ribosomal protein S15

• Macromolecule: Name: 30S ribosomal protein S15 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Acinetobacter baumannii AB0057 (bacteria) / Strain: AB0057
• Molecular weight: Theoretical: 10.1456 KDa

Sequence

String:

MALTNADRAE IIAKFARAEN DTGSPEVQVA LLTAQINDLQ GHFKAHKHDH HSRRGLIRMV NQRRKLLDYL NGKDHERYTA LIGALGLRR

UniProtKB: Small ribosomal subunit protein uS15

Macromolecule #46: 30S ribosomal protein S16

• Macromolecule: Name: 30S ribosomal protein S16 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Acinetobacter baumannii AB0057 (bacteria)
• Molecular weight: Theoretical: 11.22306 KDa

Sequence

String:

MLPMVGAGII RKNYKAFIMV VIRLARGGAK KRPFYQIVVT DSRNARDGRF IERIGFFNPT AQGQAEKLRL DADRFAHWVS QGAQPSERV ASLAAQAKKA TA

UniProtKB: Small ribosomal subunit protein bS16

Macromolecule #47: 30S ribosomal protein S17

• Macromolecule: Name: 30S ribosomal protein S17 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Acinetobacter baumannii AB0057 (bacteria) / Strain: AB0057
• Molecular weight: Theoretical: 9.543101 KDa

Sequence

String:

MSEKTVRTLT GKVVSDKMDK SIVVLIERRV QHPLYGKSIR RSTKLHAHDE NNVAKIGDVV TIKESRPISK TKAWTLVEVV EAAAE

UniProtKB: Small ribosomal subunit protein uS17

Macromolecule #48: 30S ribosomal protein S18

• Macromolecule: Name: 30S ribosomal protein S18 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Acinetobacter baumannii AB0057 (bacteria)
• Molecular weight: Theoretical: 9.009452 KDa

Sequence

String:

MARFYRRRKF CRFTAENVAY IDYKDIDTLK QYITENGKIV PSRITGTKAR YQRQLALAIK QARYLSLIPY TDNHK

UniProtKB: Small ribosomal subunit protein bS18

Macromolecule #49: 30S ribosomal protein S19

• Macromolecule: Name: 30S ribosomal protein S19 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Acinetobacter baumannii AB0057 (bacteria) / Strain: AB0057
• Molecular weight: Theoretical: 10.206957 KDa

Sequence

String:

MPRSLKKGPF VDAHLFAKVE AAVASNSRKP IKTWSRRSMI LPDFVGLTIS VHNGRNHVPV IVTEHMVGHK LGEFAPTRTY RGHGVDKKS KR

UniProtKB: Small ribosomal subunit protein uS19

Macromolecule #50: 30S ribosomal protein S20

• Macromolecule: Name: 30S ribosomal protein S20 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Acinetobacter baumannii AB0057 (bacteria) / Strain: AB0057
• Molecular weight: Theoretical: 9.72342 KDa

Sequence

String:

MANSAQAKKR ARQNVKARKH NASLRSMVRT YIKRTLSAIA GGDYAVATEA YKKAVPVIDR MADKGIIHKN KAARHKSRLN AQVKALAN

UniProtKB: Small ribosomal subunit protein bS20

Macromolecule #51: 30S ribosomal protein S21

• Macromolecule: Name: 30S ribosomal protein S21 / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Acinetobacter baumannii AB0057 (bacteria)
• Molecular weight: Theoretical: 8.474033 KDa

Sequence

String:

MPQVKLKEGE PVDVAIRRFK RSCEKAGVLA DVRKREFYEK PTQERKRKKA AAVKRYQKKL ARESVRTTRL Y

UniProtKB: Small ribosomal subunit protein bS21

Macromolecule #5: 23s ribosomal RNA

• Macromolecule: Name: 23s ribosomal RNA / type: rna / ID: 5 / Number of copies: 1
• Source (natural): Organism: Acinetobacter baumannii AB0057 (bacteria)
• Molecular weight: Theoretical: 945.31525 KDa

Sequence

String:

GUUGUAUAGU CAAGUAAUUA AGUGCAUGUG GUGGAUGCCU UGGCAGUCAG AGGCGAUGAA AGACGUGAUA GCCUGCGAAA AGCUCCGGG GAGGCGGCAA AUAUCCUUUG AUCCGGAGAU GUCUGAAUGG GGGAACCCAC CUACUUUAAG GUAGGUAUUG C AACAUGAA UACAUAGUGU UGCAAGGCGA ACGAGGGGAA GUGAAACAUC UCAGUACCCU UAGGAAAAGA AAUCAAUUGA GA UUCCCUC AGUAGCGGCG AGCGAACGGG GAUCAGCCCA UUAAGUUAUG UGUGUUUUAG UGGAACGCUC UGGGAAGUGC GAA CGUAGA GGGUGAUAUU CCCGUACACG AAAGGGCACA CAUAAUGAUG ACGAGUAGGG CGAGGCACGU GAAACCUUGU CUGA AUAUG GGGGGACCAU CCUCCAAGGC UAAAUACUCC UGACUGACCG AUAGUGAACC AGUACCGUGA GGGAAAGGCG AAAAG AACC CCUGUGAGGG GAGUGAAAUA GAUCCUGAAA CCGCAUGCAU ACAAGCAGUG GGAGCACCUU CGUGGUGUGA CUGCGU ACC UUUUGUAUAA UGGGUCAGCG ACUUAUAUUC AGUAGCGAGG UUAACCGUAU AGGGGAGCCG UAGAGAAAUC GAGUCUU AA UAGGGCGUUU AGUUGCUGGG UAUAGACCCG AAACCAGGCG AUCUAUCCAU GAGCAGGUUG AAGGUUGGGU AACACUAA C UGGAGGACCG AACCCACUGU CGUUGAAAAG CCAGGGGAUG ACUUGUGGAU AGGGGUGAAA GGCUAAUCAA GCCUGGUGA UAGCUGGUUC UCCCCGAAAG CUAUUUAGGU AGCGCCUCGG ACGAAUACCA UAGGGGGUAG AGCACUGUUU CGGCUAGGGG GUCAUCCCG ACUUACCAAA CCGAUGCAAA CUCCGAAUAC CUAUGAGUAC UAUCCGGGAG ACAGACUGCG GG(PSU)GCUA AC GUCCGUAGUC AAGAGGAAAA CAAUCCAGAC CGCCAGCUAA GGCCCCAAAA UCAUAGUUAA GUGGGAAACG AUGUGGGA A GGCAUAGACA GCUAGGAGGU UGGCUUAGAA GCAGCCACCC UUUAAAGAAA GCGUAAUAGC UCACUAGUCG AGUCGGCCU GCGCGGAAGA UGUAACGGGG CUAAAACUAU GUGCCGAAGC UGCGGAUGUA UACUUUGUAU ACGUGGUAGG GGAGCGUUCU GUAAGCCGA UGAAGGUGUG UUGAGAAGCA UGCUGGAGGU AUCAGAAGUG CGAAUGCUGA CGUGAGUAAC GACAAAACGG G UGAAAAAC CCGUUCGCCG AAAGACCAAG GGUUCCAGUC CAACGUUAAU CGGGGCUGGG UGAGUCGACC CCUAAGGCGA GG CCGAAAG GCGUAGUCGA UGGGAAAAUG GUUAAUAUUC CAUUACUUCU GUGUAAUGCG AUGAGAGGAC GGAGAAGGCU AAA UCAGCC UGGCGUUGGU UGUCCAGGUG AAAGGAUGUA GGCAUGUAUC UUAGGCAAAU CCGGGGUACU CUAUGCUGAG AUCU GAUAG CAAGCUGUAC UUGUGCAGCG AAGUGGUUGA UGCCAUGCUU CCAGGAAAAG UCUCUAAGCU UCAGUUACAC AGGAA UCGU ACCCGAAACC GACACAGGUG GUCAGGUCGA GUAGACCAAG GCGCUUGAGA GAACUCUGCU GAAGGAACUA GGCAAA AUG GUACCGUAAC UUCGGGAGAA GGUACGCUGU UGUUGGUGAU GGAACUCGCU UCCUGAGCUG ACGACAGCCG CAGAAAC CA GGCCGCUGCA ACUGUUUAUU AAAAACAUAG CACUCUGCAA ACACGAAAGU GGACGUAUAG GGUGUGAUGC CUGCCCGG U GCUGGAAGGU UAAUUGAUGG GGUUAGCGUA AGCGAAGCUC UUGAUCGAAG CCCCAGUAAA CGGCGGCCG(PSU) AAC (3TD)A(PSU)AAC GGUCCUAAGG UAGCGAAA(5MU)U CCUUGUCGGG UAAGUUCCGA CCUGCACGAA UGGCAUAAUG AU GGCGGCG CUGUCUCCAG CAGAGGCUCA GUGAAAUCGA AAUCGCUGUG (6MZ)AGAUGCAGU GUACCCGCGG CUAGACGGA AAGACCCCGU (7MG)AACCUUUAC UGCAGCUUGA CACUGAACUU UGACCUUACU UGUGUAGGAU AGGUGGGAGG CUUUGA AGC UGGAACGCUA GUUCCAGUGG AGCCGUCCUU GAAAUACCAC CCUGGUAAUG UUGAGGUUCU AACUCUGUCC CGUGAUC CG GGACGAGGAC CGUGUCUGGU GGGUAGUUUG ACUG(OMG)GGCGG UCUCCUCCUA AAGAGUAACG GAGGAGUACG AAGG UGCGC UCAGCGUGGU CGGAAAUCAC GCGUAGAGUA UAAAGGCAAA AGCGCGCUUA ACUGCGAGAC CCACAAGUCG AGCAG GUAC GAAAGUAGGU CUUAGUGAUC CGGUGGUUCU GUAUGGAAGG GCCAUCGCUC AACGGAUAAA AGGUACUCUG (2MG)G GAUAACA GGC(PSU)GAUACC GCCCAAGAGU UCAUAUCGAC GGCGGUGUUU GGCACCUCG(2MA) (PSU)GUCGGCUCA U CUCAUCCU GGGGCUGAAG CAGGUCCCAA GGGUAUGGC(OMU) GUUCGCCAUU UAAAGAGGUA CGCGAGC(PSU)GG GUUU AGAAC GUCGUGAGAC AGU(PSU)CGGUCC CUAUCUACCG UGGGCGCUGG AAAUUUGAGA GGAUCUGCUC CUAGUACGAG A GGACCAGA GUGGACGAAC CUCUGGUGUA CCGGUUGUGA CGCCAGUCGC AUCGCCGGGU AGCUAUGUUC GGAAGGGAUA AC CGCUGAA AGCAUCUAAG CGGGAAGCCU ACCUCAAGAU AAGAUUUCCC UAGGACUUUA UGUCCUCUAA AGAGCCGUUC GAG ACUAGG ACGUUGAUAG GUUGGAUGUG GAAGCAUAGU GAUAUGUGAA GCUGACCAAU ACUAAUUGCU CGUGAGGCUU GACU AUACA ACACCCAAGC AGUUG

Macromolecule #6: 5s ribosomal RNA

• Macromolecule: Name: 5s ribosomal RNA / type: rna / ID: 6 / Number of copies: 1
• Source (natural): Organism: Acinetobacter baumannii AB0057 (bacteria)
• Molecular weight: Theoretical: 36.996992 KDa

Sequence

String:

GCUGGCGACC AUAGCAAGAG UGAACCACCU GAUCCCUUCC CGAACUCAGA AGUGAAACCU CUUAGCGCUG AUGGUAGUGU GGGAUUACC CAUGUGAGAG UAAGUCAUCG CCAGCU

GENBANK: GENBANK: CP035930.1

Macromolecule #31: 16s Ribosomal RNA

• Macromolecule: Name: 16s Ribosomal RNA / type: rna / ID: 31 / Number of copies: 1
• Source (natural): Organism: Acinetobacter baumannii AB0057 (bacteria)
• Molecular weight: Theoretical: 500.297531 KDa

Sequence

String:

UUUAACUGAA GAGUUUGAUC AUGGCUCAGA UUGAACGCUG GCGGCAGGCU UAACACAUGC AAGUCGAGCG GGGGAAGGUA GCUUGCUAC CGGACCUAGC GGCGGACGGG UGAGUAAUGC UUAGGAAUCU GCCUAUUAGU GGGGGACAAC AUCUCGAAAG G GAUGCUAA UACCGCAUAC GUCCUACGGG AGAAAGCAGG GGAUCUUCGG ACCUUGCGCU AAUAGAUGAG CCUAAGUCGG AU UAGCUAG UUGGUGGGGU AAAGGCCUAC CAAGGCGACG AUCUGUAGCG GGUCUGAGAG GAUGAUCCGC CACACUGGGA CUG AGACAC GGCCCAGACU CCUACGGGAG GCAGCAGUGG GGAAUAUUGG ACAAUGGGGG GAACCCUGAU CCAGCCAUGC CGCG UGUGU GAAGAAGGCC UUAUGGUUGU AAAGCACUUU AAGCGAGGAG GAGGCUACUC UAGUUAAUAC CUAGGGAUAG UGGAC GUUA CUCGCAGAAU AAGCACCGGC UAACUCUG(PSU)G CCAGCAGCC(7MG) CGGUAAUACA GAGGGUGCGA GCGUUAAU C GGAUUUACUG GGCGUAAAGC GUGCGUAGGC GGCUUAUUAA GUCGGAUGUG AAAUCCCCGA GCUUAACUUG GGAAUUGCA UUCGAUACUG GUGAGCUAGA GUAUGGGAGA GGAUGGUAGA AUUCCAGGUG UAGCGGUGAA AUGCGUAGAG AUCUGGAGGA AUACCGAUG GCGAAGGCAG CCAUCUGGCC UAAUACUGAC GCUGAGGUAC GAAAGCAUGG GGAGCAAACA GGAUUAGAUA C CCUGGUAG UCCAUGCCGU AAACGAUGUC UACUAGCCGU UGGGGCCUUU GAGGCUUUAG UGGCGCAGCU AACGCGAUAA GU AGACCGC CUGGGGAGUA CGGUCGCAAG ACUAAAACUC AAAUGAAUUG ACGGGGGCCC GCACAAGCGG UGGAGCAUGU GGU UUAAUU CGAU(2MG)(5MC)AACG CGAAGAACCU UACCUGGCCU UGACAUACUA GAAACUUUUC AGAGAUGGAU UGGUGC CUU CGGGAACCUA GAUACAGGUG CUGCAUGGCU GUCGUCAGCU CGUGUCGUGA GAUGUUGGGU UAAGUCCCGC AACGAGC GC AACCCUUUUC CUUACUUGCC AGCAUUUCGG AUGGGAACUU UAAGGAUACU GCCAGUGACA AACUGGAGGA AGGCGGGG A CGACGUCAAG UCAUCAUG(2MG)C CCUUACGGCC AGGGCUACAC ACGUGCUACA AUGGUCGGUA CAAAGGGUUG CUACA CAGC GAUGUGAUGC UAAUCUCAAA AAGCCGAUCG UAGUCCGGAU UGGAGUCUGC AACUCGACUC CAUGAAGUCG GAAUCG CUA GUAAUCGCGG AUCAGAAUGC CGCGGUGAAU ACGUUCCCGG GCCUUGUACA CACCG(4OC)CCGU CACACCAUGG GAG UUUGUU GCACCAGAAG UAGCUAGCCU AACUGCAAAG AGGGCGGUUA CCACGGUGUG GCCGAUGACU GGGGUGAAGU CG (UR3)AACAAG GUAGCCGUAG GGG(MA6)(MA6)CCUGC GGCUGGAUCA CCUCCUUAAC GAA

GENBANK: GENBANK: CP001182.2

Macromolecule #52: tRNA-met

• Macromolecule: Name: tRNA-met / type: rna / ID: 52 / Number of copies: 1
• Source (natural): Organism: Acinetobacter baumannii AB0057 (bacteria)
• Molecular weight: Theoretical: 24.760799 KDa

Sequence

String:

GGCUAUG(4SU)AG CUCAGUUGG(H2U) UAGAGCACCG CACUCAUAAU GCGGGGGUCA CAAG(5MU)(PSU)CAAG UCUC GUCAU AGCCACCA

GENBANK: GENBANK: CP026750.2

Macromolecule #53: mRNA

• Macromolecule: Name: mRNA / type: rna / ID: 53 / Number of copies: 1
• Source (natural): Organism: Acinetobacter baumannii AB0057 (bacteria)
• Molecular weight: Theoretical: 935.62 Da

Sequence

String:

AUG

Macromolecule #54: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 54 / Number of copies: 1 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Macromolecule #55: Streptothricin D

• Macromolecule: Name: Streptothricin D / type: ligand / ID: 55 / Number of copies: 11 / Formula: OIY
• Molecular weight: Theoretical: 758.867 Da

Chemical component information

ChemComp-OIY:
Streptothricin D

Macromolecule #56: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 56 / Number of copies: 1 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Macromolecule #57: water

• Macromolecule: Name: water / type: ligand / ID: 57 / Number of copies: 5 / Formula: HOH
• Molecular weight: Theoretical: 18.015 Da

Chemical component information

ChemComp-HOH:
WATER

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Buffer: pH: 7.4
• Vitrification: Cryogen name: ETHANE

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Image recording: Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 36.0 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: SPOT SCAN / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 1.0 µm
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• Startup model: Type of model: INSILICO MODEL / In silico model: ab inito
• Final reconstruction: Resolution.type: BY AUTHOR / Resolution: 2.21 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 451971
• Initial angle assignment: Type: MAXIMUM LIKELIHOOD
• Final angle assignment: Type: MAXIMUM LIKELIHOOD