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- EMDB-26450: Pinch-off CHIKV assembly/budding intermediate from virus-infected... -
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Open data
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Basic information
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Title | Pinch-off CHIKV assembly/budding intermediate from virus-infected human cells | ||||||||||||
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![]() | alphavirus / icosahedral / enveloped virus / VIRUS | ||||||||||||
Biological species | ![]() ![]() | ||||||||||||
Method | subtomogram averaging / cryo EM / Resolution: 13.3 Å | ||||||||||||
![]() | Chmielewski D / Schmid MF / Jin J / Simmons G / Chiu W | ||||||||||||
Funding support | ![]()
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![]() | ![]() Title: In situ Alphavirus Assembly and Budding Mechanism Revealed by Cellular CryoET Authors: Chmielewski D / Schmid MF / Jin J / Simmons G / Chiu W | ||||||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 1.4 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 13.5 KB 13.5 KB | Display Display | ![]() |
Images | ![]() | 143.2 KB | ||
Filedesc metadata | ![]() | 4 KB | ||
Others | ![]() ![]() | 1.7 MB 1.7 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 669.5 KB | Display | ![]() |
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Full document | ![]() | 669 KB | Display | |
Data in XML | ![]() | 9.6 KB | Display | |
Data in CIF | ![]() | 11.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | C: citing same article ( |
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||
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Voxel size | X=Y=Z: 3.54 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: #1
File | emd_26450_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #2
File | emd_26450_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
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Sample components
-Entire : Chikungunya virus
Entire | Name: ![]() ![]() |
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Components |
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-Supramolecule #1: Chikungunya virus
Supramolecule | Name: Chikungunya virus / type: virus / ID: 1 / Parent: 0 / NCBI-ID: 37124 / Sci species name: Chikungunya virus / Virus type: VIRION / Virus isolate: SPECIES / Virus enveloped: Yes / Virus empty: No |
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Virus shell | Shell ID: 1 / Diameter: 700.0 Å / T number (triangulation number): 4 |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | subtomogram averaging |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 7 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TALOS ARCTICA |
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Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 2.0 e/Å2 |
Electron beam | Acceleration voltage: 200 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: -0.005 µm / Nominal defocus min: -0.002 µm |
Experimental equipment | ![]() Model: Talos Arctica / Image courtesy: FEI Company |
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Image processing
Final reconstruction | Applied symmetry - Point group: C5 (5 fold cyclic) / Resolution.type: BY AUTHOR / Resolution: 13.3 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: EMAN2 / Number subtomograms used: 354 |
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Extraction | Number tomograms: 185 / Number images used: 354 |
Final angle assignment | Type: PROJECTION MATCHING |