ジャーナル: Cytoskeleton (Hoboken) / 年: 2014 タイトル: Structure of dimeric axonemal dynein in cilia suggests an alternative mechanism of force generation. 著者: Hironori Ueno / Khanh Huy Bui / Takuji Ishikawa / Yohsuke Imai / Takami Yamaguchi / Takashi Ishikawa / 要旨: The mechanism by which the two different heads of the ciliary outer dynein arm produce force to translocate the microtubule during beating is still unknown. In this report we use cryo-electron ...The mechanism by which the two different heads of the ciliary outer dynein arm produce force to translocate the microtubule during beating is still unknown. In this report we use cryo-electron tomography and image processing to analyze the conformational changes and the relative abundance of each conformation of the two dynein heads from mouse respiratory cilia. In the absence of nucleotides the majority of dynein dimers are in the apo form and both heads are tightly packed, whereas they are dissociated and move independently in the presence of nucleotides. The head of the external outer arm dynein heavy chain has a diagonal shift toward both the neighboring B-tubule and the proximal end of the axoneme, while the head of the internal heavy chain shifts only longitudinally toward the proximal end. In the presence of nucleotides a significant number of the dynein dimers have two heads overlapped in the proximal shifting form or overlapped in the apo form. During ciliary bending axonemal dynein translocates microtubules by moving with short steps and two heads stay at the same position longer than cytoplasmic dynein. This demonstrates that the step of the outer arm dynein dimer is not dominated by the hand-over-hand motion, but also indicates the difference between axonemal dynein and cytoplasmic dynein.
ダウンロード / ファイル: emd_2632.map.gz / 形式: CCP4 / 大きさ: 11.9 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
注釈
averaged subtomograms of outer arm dynein dimers from mouse respiratory cilia. Classified as an APO form.
ボクセルのサイズ
X=Y=Z: 7.25 Å
密度
表面レベル
登録者による: 0.25 / ムービー #1: 0.25
最小 - 最大
-7.18062878 - 10.31612206
平均 (標準偏差)
0.00495047 (±1.03347766)
対称性
空間群: 1
詳細
EMDB XML:
マップ形状
Axis order
X
Y
Z
Origin
0
0
6
サイズ
200
200
80
Spacing
200
200
80
セル
A: 1450.0 Å / B: 1450.0 Å / C: 580.0 Å α=β=γ: 90.0 °
CCP4マップ ヘッダ情報:
mode
Image stored as Reals
Å/pix. X/Y/Z
7.25
7.25
7.25
M x/y/z
200
200
80
origin x/y/z
0.000
0.000
0.000
length x/y/z
1450.000
1450.000
580.000
α/β/γ
90.000
90.000
90.000
start NX/NY/NZ
-184
-184
-183
NX/NY/NZ
368
368
368
MAP C/R/S
1
2
3
start NC/NR/NS
0
0
6
NC/NR/NS
200
200
80
D min/max/mean
-7.181
10.316
0.005
-
添付データ
-
試料の構成要素
-
全体 : Outer arm dynein dimer from mouse respiratory cilia in the absenc...
全体
名称: Outer arm dynein dimer from mouse respiratory cilia in the absence of additional nucleotides
要素
試料: Outer arm dynein dimer from mouse respiratory cilia in the absence of additional nucleotides
細胞器官・細胞要素: outer arm dyneins with a microtubule doublet
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超分子 #1000: Outer arm dynein dimer from mouse respiratory cilia in the absenc...
超分子
名称: Outer arm dynein dimer from mouse respiratory cilia in the absence of additional nucleotides タイプ: sample / ID: 1000 / 詳細: in situ sample / Number unique components: 1
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超分子 #1: outer arm dyneins with a microtubule doublet
超分子
名称: outer arm dyneins with a microtubule doublet / タイプ: organelle_or_cellular_component / ID: 1 / 集合状態: dimer / 組換発現: No