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Open data
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Basic information
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Title | Human KCC1 bound with VU0463271 In an outward-open state | |||||||||
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Function / homology | ![]() ammonium import across plasma membrane / potassium:chloride symporter activity / Cation-coupled Chloride cotransporters / chloride ion homeostasis / ammonium channel activity / potassium ion homeostasis / cell volume homeostasis / potassium ion import across plasma membrane / monoatomic ion transport / chloride transmembrane transport ...ammonium import across plasma membrane / potassium:chloride symporter activity / Cation-coupled Chloride cotransporters / chloride ion homeostasis / ammonium channel activity / potassium ion homeostasis / cell volume homeostasis / potassium ion import across plasma membrane / monoatomic ion transport / chloride transmembrane transport / potassium ion transmembrane transport / chemical synaptic transmission / lysosomal membrane / synapse / protein kinase binding / ATP binding / membrane / plasma membrane Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.5 Å | |||||||||
![]() | Zhao YX / Cao EH | |||||||||
Funding support | 1 items
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![]() | ![]() Title: Structure of the human cation-chloride cotransport KCC1 in an outward-open state. Authors: Yongxiang Zhao / Jiemin Shen / Qinzhe Wang / Manuel Jose Ruiz Munevar / Pietro Vidossich / Marco De Vivo / Ming Zhou / Erhu Cao / ![]() ![]() Abstract: Cation-chloride cotransporters (CCCs) catalyze electroneutral symport of Cl with Na and/or K across membranes. CCCs are fundamental in cell volume homeostasis, transepithelia ion movement, ...Cation-chloride cotransporters (CCCs) catalyze electroneutral symport of Cl with Na and/or K across membranes. CCCs are fundamental in cell volume homeostasis, transepithelia ion movement, maintenance of intracellular Cl concentration, and neuronal excitability. Here, we present a cryoelectron microscopy structure of human K-Cl cotransporter (KCC)1 bound with the VU0463271 inhibitor in an outward-open state. In contrast to many other amino acid-polyamine-organocation transporter cousins, our first outward-open CCC structure reveals that opening the KCC1 extracellular ion permeation path does not involve hinge-bending motions of the transmembrane (TM) 1 and TM6 half-helices. Instead, rocking of TM3 and TM8, together with displacements of TM4, TM9, and a conserved intracellular loop 1 helix, underlie alternate opening and closing of extracellular and cytoplasmic vestibules. We show that KCC1 intriguingly exists in one of two distinct dimeric states via different intersubunit interfaces. Our studies provide a blueprint for understanding the mechanisms of CCCs and their inhibition by small molecule compounds. | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 59.7 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 13.2 KB 13.2 KB | Display Display | ![]() |
Images | ![]() | 30.5 KB | ||
Others | ![]() | 59.2 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7ttiMC ![]() 7tthC C: citing same article ( M: atomic model generated by this map |
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Similar structure data | Similarity search - Function & homology ![]() |
EM raw data | ![]() Data size: 3.9 TB Data #1: HumanKCC1-VU0463271 complex [micrographs - multiframe]) |
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Links
EMDB pages | ![]() ![]() |
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Map
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Voxel size | X=Y=Z: 1.035 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Additional map: #1
File | emd_26116_additional_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
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Sample components
-Entire : human potassium-chloride cotransporter 1 bound with VU0463271
Entire | Name: human potassium-chloride cotransporter 1 bound with VU0463271 |
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Components |
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-Supramolecule #1: human potassium-chloride cotransporter 1 bound with VU0463271
Supramolecule | Name: human potassium-chloride cotransporter 1 bound with VU0463271 type: complex / Chimera: Yes / ID: 1 / Parent: 0 / Macromolecule list: #1 |
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Source (natural) | Organism: ![]() |
Recombinant expression | Organism: ![]() |
-Macromolecule #1: Solute carrier family 12 member 4
Macromolecule | Name: Solute carrier family 12 member 4 / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 120.770492 KDa |
Recombinant expression | Organism: ![]() |
Sequence | String: MPHFTVVPVD GPRRGDYDNL EGLSWVDYGE RAELDDSDGH GNHRESSPFL SPLEASRGID YYDRNLALFE EELDIRPKVS SLLGKLVSY TNLTQGAKEH EEAESGEGTR RRAAEAPSMG TLMGVYLPCL QNIFGVILFL RLTWMVGTAG VLQALLIVLI C CCCTLLTA ...String: MPHFTVVPVD GPRRGDYDNL EGLSWVDYGE RAELDDSDGH GNHRESSPFL SPLEASRGID YYDRNLALFE EELDIRPKVS SLLGKLVSY TNLTQGAKEH EEAESGEGTR RRAAEAPSMG TLMGVYLPCL QNIFGVILFL RLTWMVGTAG VLQALLIVLI C CCCTLLTA ISMSAIATNG VVPAGGSYFM ISRSLGPEFG GAVGLCFYLG TTFAAAMYIL GAIEILLTYI APPAAIFYPS GA HDTSNAT LNNMRVYGTI FLTFMTLVVF VGVKYVNKFA SLFLACVIIS ILSIYAGGIK SIFDPPVFPV CMLGNRTLSR DQF DICAKT AVVDNETVAT QLWSFFCHSP NLTTDSCDPY FMLNNVTEIP GIPGAAAGVL QENLWSAYLE KGDIVEKHGL PSAD APSLK ESLPLYVVAD IATSFTVLVG IFFPSVTGIM AGSNRSGDLR DAQKSIPVGT ILAIITTSLV YFSSVVLFGA CIEGV VLRD KYGDGVSRNL VVGTLAWPSP WVIVIGSFFS TCGAGLQSLT GAPRLLQAIA KDNIIPFLRV FGHGKVNGEP TWALLL TAL IAELGILIAS LDMVAPILSM FFLMCYLFVN LACAVQTLLR TPNWRPRFKY YHWALSFLGM SLCLALMFVS SWYYALV AM LIAGMIYKYI EYQGAEKEWG DGIRGLSLSA ARYALLRLEE GPPHTKNWRP QLLVLLKLDE DLHVKYPRLL TFASQLKA G KGLTIVGSVI QGSFLESYGE AQAAEQTIKN MMEIEKVKGF CQVVVASKVR EGLAHLIQSC GLGGMRHNSV VLGWPYGWR QSEDPRAWKT FIDTVRCTTA AHLALLVPKN IAFYPSNHER YLEGHIDVWW IVHDGGMLML LPFLLRQHKV WRKCRMRIFT VAQMDDNSI QMKKDLAVFL YHLRLEAEVE VVEMHNSDIS AYTYERTLMM EQRSQMLRQM RLTKTERERE AQLVKDRHSA L RLESLYSD EEDESAVGAD KIQMTWTRDK YMTETWDPSH APDNFRELVH IKPDQSNVRR MHTAVKLNEV IVTRSHDARL VL LNMPGPP RNSEGDENYM EFLEVLTEGL ERVLLVRGGG REVITIYS |
-Macromolecule #3: N-cyclopropyl-N-(4-methyl-1,3-thiazol-2-yl)-2-[(6-phenylpyridazin...
Macromolecule | Name: N-cyclopropyl-N-(4-methyl-1,3-thiazol-2-yl)-2-[(6-phenylpyridazin-3-yl)sulfanyl]acetamide type: ligand / ID: 3 / Number of copies: 2 / Formula: JUX |
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Molecular weight | Theoretical: 382.502 Da |
Chemical component information | ![]() ChemComp-JUX: |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 7.4 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 50.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: DIFFRACTION / Nominal defocus max: 3.5 µm / Nominal defocus min: 0.7000000000000001 µm |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 3.5 Å / Resolution method: DIFFRACTION PATTERN/LAYERLINES / Number images used: 81855 |
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Initial angle assignment | Type: ANGULAR RECONSTITUTION |
Final angle assignment | Type: ANGULAR RECONSTITUTION |