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- EMDB-26067: CryoEM structure of the human 40S small ribosomal subunit in comp... -

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Basic information

Entry
Database: EMDB / ID: EMD-26067
TitleCryoEM structure of the human 40S small ribosomal subunit in complex with translation initiation factors eIF1A and eIF5B.
Map dataSharpened map
Sample
  • Cell: human 40S ribosomal subunit in complex with eIF1A and eIF5B
    • RNA: x 2 types
    • Protein or peptide: x 31 types
  • Protein or peptide: x 4 types
  • Ligand: x 3 types
KeywordseIF5B / translation / initiation / eIF1A / RIBOSOME
Function / homology
Function and homology information


negative regulation of endoplasmic reticulum unfolded protein response / oxidized pyrimidine DNA binding / response to TNF agonist / positive regulation of base-excision repair / negative regulation of peptidyl-serine phosphorylation / positive regulation of respiratory burst involved in inflammatory response / positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage / positive regulation of gastrulation / regulation of adenylate cyclase-activating G protein-coupled receptor signaling pathway / protein tyrosine kinase inhibitor activity ...negative regulation of endoplasmic reticulum unfolded protein response / oxidized pyrimidine DNA binding / response to TNF agonist / positive regulation of base-excision repair / negative regulation of peptidyl-serine phosphorylation / positive regulation of respiratory burst involved in inflammatory response / positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage / positive regulation of gastrulation / regulation of adenylate cyclase-activating G protein-coupled receptor signaling pathway / protein tyrosine kinase inhibitor activity / IRE1-RACK1-PP2A complex / positive regulation of endodeoxyribonuclease activity / nucleolus organization / positive regulation of Golgi to plasma membrane protein transport / translation at postsynapse / TNFR1-mediated ceramide production / protein-synthesizing GTPase / negative regulation of DNA repair / negative regulation of RNA splicing / mammalian oogenesis stage / regulation of translational initiation / supercoiled DNA binding / activation-induced cell death of T cells / neural crest cell differentiation / NF-kappaB complex / oxidized purine DNA binding / cysteine-type endopeptidase activator activity involved in apoptotic process / negative regulation of intrinsic apoptotic signaling pathway in response to hydrogen peroxide / ubiquitin-like protein conjugating enzyme binding / regulation of establishment of cell polarity / translation at presynapse / positive regulation of ubiquitin-protein transferase activity / Formation of the ternary complex, and subsequently, the 43S complex / negative regulation of phagocytosis / erythrocyte homeostasis / rRNA modification in the nucleus and cytosol / cytoplasmic side of rough endoplasmic reticulum membrane / protein kinase A binding / negative regulation of ubiquitin protein ligase activity / pigmentation / Ribosomal scanning and start codon recognition / ion channel inhibitor activity / Translation initiation complex formation / positive regulation of mitochondrial depolarization / positive regulation of T cell receptor signaling pathway / positive regulation of activated T cell proliferation / fibroblast growth factor binding / negative regulation of Wnt signaling pathway / monocyte chemotaxis / negative regulation of translational frameshifting / Protein hydroxylation / BH3 domain binding / TOR signaling / SARS-CoV-1 modulates host translation machinery / regulation of cell division / mTORC1-mediated signalling / T cell proliferation involved in immune response / Peptide chain elongation / iron-sulfur cluster binding / positive regulation of intrinsic apoptotic signaling pathway by p53 class mediator / Selenocysteine synthesis / positive regulation of signal transduction by p53 class mediator / Formation of a pool of free 40S subunits / endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / ubiquitin ligase inhibitor activity / Eukaryotic Translation Termination / Response of EIF2AK4 (GCN2) to amino acid deficiency / SRP-dependent cotranslational protein targeting to membrane / negative regulation of ubiquitin-dependent protein catabolic process / Viral mRNA Translation / negative regulation of respiratory burst involved in inflammatory response / phagocytic cup / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / GTP hydrolysis and joining of the 60S ribosomal subunit / L13a-mediated translational silencing of Ceruloplasmin expression / erythrocyte development / Major pathway of rRNA processing in the nucleolus and cytosol / regulation of translational fidelity / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / Protein methylation / spindle assembly / Nuclear events stimulated by ALK signaling in cancer / ribosomal small subunit export from nucleus / positive regulation of intrinsic apoptotic signaling pathway / rough endoplasmic reticulum / translation regulator activity / Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal / positive regulation of cell cycle / translation initiation factor binding / signaling adaptor activity / gastrulation / Maturation of protein E / Maturation of protein E / MDM2/MDM4 family protein binding / positive regulation of microtubule polymerization / cytosolic ribosome / ER Quality Control Compartment (ERQC) / Mitotic Prometaphase / DNA-(apurinic or apyrimidinic site) endonuclease activity
Similarity search - Function
Elongation factor Tu-type domain / Elongation factor Tu domain 4 / Translation initiation factor IF- 2, domain 3 / Translation-initiation factor 2 / Translation initiation factor IF- 2 / Translation initiation factor IF-2, domain 3 superfamily / : / Ribosomal protein S26e signature. / Ribosomal protein S21e, conserved site / Ribosomal protein S21e signature. ...Elongation factor Tu-type domain / Elongation factor Tu domain 4 / Translation initiation factor IF- 2, domain 3 / Translation-initiation factor 2 / Translation initiation factor IF- 2 / Translation initiation factor IF-2, domain 3 superfamily / : / Ribosomal protein S26e signature. / Ribosomal protein S21e, conserved site / Ribosomal protein S21e signature. / Ribosomal protein S26e / Ribosomal protein S26e superfamily / Ribosomal protein S26e / Ribosomal protein S19e, conserved site / Ribosomal protein S19e signature. / Small (40S) ribosomal subunit Asc1/RACK1 / Ribosomal protein S5, eukaryotic/archaeal / Ribosomal protein S21e / Ribosomal protein S21e superfamily / Ribosomal protein S21e / S27a-like superfamily / 40S Ribosomal protein S10 / : / Ribosomal protein S7e signature. / Plectin/S10, N-terminal / Plectin/S10 domain / Ribosomal protein S8e subdomain, eukaryotes / Ribosomal protein S27a / Ribosomal protein S27a / Ribosomal protein S27a / Ribosomal protein S27e signature. / 40S ribosomal protein S4, C-terminal domain / 40S ribosomal protein S4 C-terminus / Ribosomal protein S4e, N-terminal, conserved site / Ribosomal protein S4e signature. / Ribosomal protein S3, eukaryotic/archaeal / Ribosomal protein S19e / Ribosomal protein S8e, conserved site / Ribosomal protein S19e / Ribosomal protein S8e signature. / Ribosomal_S19e / Ribosomal protein S6, eukaryotic / Ribosomal protein S7e / Ribosomal protein S7e / Ribosomal protein S19A/S15e / 40S ribosomal protein S11, N-terminal / Ribosomal_S17 N-terminal / : / Ribosomal S24e conserved site / Ribosomal protein S24e signature. / Ribosomal protein S4e, N-terminal / RS4NT (NUC023) domain / Ribosomal protein S4, KOW domain / Ribosomal protein S4e / Ribosomal protein S4e, central region / Ribosomal protein S4e, central domain superfamily / Ribosomal family S4e / Ribosomal protein S23, eukaryotic/archaeal / Ribosomal protein S6/S6e/A/B/2, conserved site / Ribosomal protein S6e signature. / Ribosomal protein S24e / Ribosomal protein S24e / Ribosomal protein S27 / Ribosomal protein S27, zinc-binding domain superfamily / Ribosomal protein S27 / Ribosomal protein S17, archaeal/eukaryotic / Ribosomal protein S8e / Ribosomal protein S6e / Ribosomal protein S6e / Ribosomal protein S5/S7, eukaryotic/archaeal / Ribosomal protein S6e / Ribosomal protein S4/S9, eukaryotic/archaeal / Translational (tr)-type GTP-binding domain / Elongation factor Tu GTP binding domain / Translational (tr)-type guanine nucleotide-binding (G) domain profile. / Ribosomal protein S8e/ribosomal biogenesis NSA2 / Ribosomal protein S8e / : / Ubiquitin domain signature. / Ubiquitin conserved site / Ubiquitin domain / Ribosomal protein S3, conserved site / Ribosomal protein S3 signature. / KH domain / Type-2 KH domain profile. / K Homology domain, type 2 / Ribosomal protein S3, C-terminal / Ribosomal protein S3, C-terminal domain / Ribosomal protein S3, C-terminal domain superfamily / Ribosomal protein S15/S19, conserved site / Ribosomal protein S19 signature. / Ribosomal protein S19/S15 / Ribosomal protein S19/S15, superfamily / Ribosomal protein S19 / : / Ribosomal protein S5, N-terminal, conserved site / Ribosomal protein S5 signature. / Ribosomal protein S7, conserved site / Ribosomal protein S7 signature. / Ubiquitin family
Similarity search - Domain/homology
Small ribosomal subunit protein eS10 / Eukaryotic translation initiation factor 5B / Small ribosomal subunit protein uS5 / Small ribosomal subunit protein uS3 / Small ribosomal subunit protein eS19 / Small ribosomal subunit protein eS27 / Small ribosomal subunit protein uS4 / Small ribosomal subunit protein uS7 / Small ribosomal subunit protein eS7 / Small ribosomal subunit protein eS8 ...Small ribosomal subunit protein eS10 / Eukaryotic translation initiation factor 5B / Small ribosomal subunit protein uS5 / Small ribosomal subunit protein uS3 / Small ribosomal subunit protein eS19 / Small ribosomal subunit protein eS27 / Small ribosomal subunit protein uS4 / Small ribosomal subunit protein uS7 / Small ribosomal subunit protein eS7 / Small ribosomal subunit protein eS8 / Small ribosomal subunit protein uS8 / Small ribosomal subunit protein uS9 / Small ribosomal subunit protein uS12 / Small ribosomal subunit protein uS17 / Small ribosomal subunit protein eS4, X isoform / Small ribosomal subunit protein eS6 / Small ribosomal subunit protein uS19 / Small ribosomal subunit protein eS24 / Small ribosomal subunit protein eS26 / Ubiquitin-ribosomal protein eS31 fusion protein / Small ribosomal subunit protein eS21 / Small ribosomal subunit protein RACK1
Similarity search - Component
Biological speciesHomo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.2 Å
AuthorsLapointe CP / Grosely R
Funding support United States, Sweden, 3 items
OrganizationGrant numberCountry
Damon Runyon Cancer Research FoundationDRG-#2321-18 United States
Knut and Alice Wallenberg Foundation(KAW2015.0406 Sweden
National Institutes of Health/Eunice Kennedy Shriver National Institute of Child Health & Human Development (NIH/NICHD)(GM011378 United States
CitationJournal: Nature / Year: 2022
Title: eIF5B and eIF1A reorient initiator tRNA to allow ribosomal subunit joining.
Authors: Christopher P Lapointe / Rosslyn Grosely / Masaaki Sokabe / Carlos Alvarado / Jinfan Wang / Elizabeth Montabana / Nancy Villa / Byung-Sik Shin / Thomas E Dever / Christopher S Fraser / ...Authors: Christopher P Lapointe / Rosslyn Grosely / Masaaki Sokabe / Carlos Alvarado / Jinfan Wang / Elizabeth Montabana / Nancy Villa / Byung-Sik Shin / Thomas E Dever / Christopher S Fraser / Israel S Fernández / Joseph D Puglisi /
Abstract: Translation initiation defines the identity and quantity of a synthesized protein. The process is dysregulated in many human diseases. A key commitment step is when the ribosomal subunits join at a ...Translation initiation defines the identity and quantity of a synthesized protein. The process is dysregulated in many human diseases. A key commitment step is when the ribosomal subunits join at a translation start site on a messenger RNA to form a functional ribosome. Here, we combined single-molecule spectroscopy and structural methods using an in vitro reconstituted system to examine how the human ribosomal subunits join. Single-molecule fluorescence revealed when the universally conserved eukaryotic initiation factors eIF1A and eIF5B associate with and depart from initiation complexes. Guided by single-molecule dynamics, we visualized initiation complexes that contained both eIF1A and eIF5B using single-particle cryo-electron microscopy. The resulting structure revealed how eukaryote-specific contacts between the two proteins remodel the initiation complex to orient the initiator aminoacyl-tRNA in a conformation compatible with ribosomal subunit joining. Collectively, our findings provide a quantitative and architectural framework for the molecular choreography orchestrated by eIF1A and eIF5B during translation initiation in humans.
History
DepositionJan 26, 2022-
Header (metadata) releaseApr 27, 2022-
Map releaseApr 27, 2022-
UpdateJun 12, 2024-
Current statusJun 12, 2024Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_26067.png

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Map

FileDownload / File: emd_26067.map.gz / Format: CCP4 / Size: 421.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationSharpened map
Projections & slices

Image control

Size
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AxesZ (Sec.)Y (Row.)X (Col.)
1.31 Å/pix.
x 480 pix.
= 628.8 Å		1.31 Å/pix.
x 480 pix.
= 628.8 Å		1.31 Å/pix.
x 480 pix.
= 628.8 Å

Surface

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Images are generated by Spider.

Voxel sizeX=Y=Z: 1.31 Å
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Histogram (log scale)
Contour LevelBy AUTHOR: 0.1
Minimum - Maximum-0.031308204 - 2.5343385
Average (Standard dev.)0.0004691698 (±0.022956336)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions480480480
Spacing480480480
CellA=B=C: 628.8 Å
α=β=γ: 90.0 °

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Supplemental data

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Mask #1

Fileemd_26067_msk_1.map
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Additional map: Unsharpened map

Fileemd_26067_additional_1.map
AnnotationUnsharpened map
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Half map: Half map 1

Fileemd_26067_half_map_1.map
AnnotationHalf map 1
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Half map: Half map 2

Fileemd_26067_half_map_2.map
AnnotationHalf map 2
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Sample components

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Entire : human 40S ribosomal subunit in complex with eIF1A and eIF5B

EntireName: human 40S ribosomal subunit in complex with eIF1A and eIF5B
Components
  • Cell: human 40S ribosomal subunit in complex with eIF1A and eIF5B
    • RNA: 18S ribosomal RNA
    • RNA: Human Met-tRNAiMet
    • Protein or peptide: Translation initiation factor eIF1A
    • Protein or peptide: ribosomal protein uS2
    • Protein or peptide: 40S ribosomal protein S2
    • Protein or peptide: 40S ribosomal protein S4, X isoform
    • Protein or peptide: 40S ribosomal protein S3
    • Protein or peptide: 40S ribosomal protein S6
    • Protein or peptide: 40S ribosomal protein S7
    • Protein or peptide: 40S ribosomal protein S8
    • Protein or peptide: 40S ribosomal protein S9
    • Protein or peptide: 40S ribosomal protein S5
    • Protein or peptide: 40S ribosomal protein S11
    • Protein or peptide: 40S ribosomal protein S10
    • Protein or peptide: ribosomal protein uS15
    • Protein or peptide: ribosomal protein eS12
    • Protein or peptide: ribosomal protein uS11
    • Protein or peptide: 40S ribosomal protein S15
    • Protein or peptide: 40S ribosomal protein S16
    • Protein or peptide: ribosomal protein eS17
    • Protein or peptide: ribosomal protein uS13
    • Protein or peptide: 40S ribosomal protein S19
    • Protein or peptide: ribosomal protein uS10
    • Protein or peptide: 40S ribosomal protein S15a
    • Protein or peptide: 40S ribosomal protein S23
    • Protein or peptide: Isoform 3 of 40S ribosomal protein S24
    • Protein or peptide: 40S ribosomal protein S21
    • Protein or peptide: ribosomal protein eS25
    • Protein or peptide: 40S ribosomal protein S27
    • Protein or peptide: ribosomal protein eS28
    • Protein or peptide: ribosomal protein eS30
    • Protein or peptide: ribosomal protein eL41
    • Protein or peptide: Receptor of activated protein C kinase 1
  • Protein or peptide: Eukaryotic translation initiation factor 5B
  • Protein or peptide: 40S ribosomal protein S26
  • Protein or peptide: ribosomal protein uS14
  • Protein or peptide: 40S ribosomal protein S27a
  • Ligand: PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER
  • Ligand: GUANOSINE-5'-MONOPHOSPHATE
  • Ligand: ZINC ION

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Supramolecule #1: human 40S ribosomal subunit in complex with eIF1A and eIF5B

SupramoleculeName: human 40S ribosomal subunit in complex with eIF1A and eIF5B
type: cell / ID: 1 / Parent: 0 / Macromolecule list: #2-#30, #32-#33, #36-#37
Source (natural)Organism: Homo sapiens (human)

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Macromolecule #1: Eukaryotic translation initiation factor 5B

MacromoleculeName: Eukaryotic translation initiation factor 5B / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO / EC number: protein-synthesizing GTPase
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 69.868062 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: EERAYDKAKR RIEKRRLEHS KNVTEKDKLR APIICVLGHV DTGKTKILDK LRHTHVQDGE AGGITQQIGA TNVPLEAINE QTKMIKNFD RENVRIPGML IIDTPGHESF SNLRNRGSSL CDIAILVVDI MHGLEPQTIE SINLLKSKKC PFIVALNKID R LYDWKKSP ...String:
EERAYDKAKR RIEKRRLEHS KNVTEKDKLR APIICVLGHV DTGKTKILDK LRHTHVQDGE AGGITQQIGA TNVPLEAINE QTKMIKNFD RENVRIPGML IIDTPGHESF SNLRNRGSSL CDIAILVVDI MHGLEPQTIE SINLLKSKKC PFIVALNKID R LYDWKKSP DSDVAATLKK QKKNTKDEFE ERAKAIIVEF AQQGLNAALF YENKDPRTFV SLVPTSAHTG DGMGSLIYLL VE LTQTMLS KRLAHCEELR AQVMEVKALP GMGTTIDVIL INGRLKEGDT IIVPGVEGPI VTQIRGLLTP PPMKELRVKN QYE KHKEVE AAQGVKILGK DLEKTLAGLP LLVAYKEDEI PVLKDELIHE LKQTLNAIKL EEKGVYVQAS TLGSLEALLE FLKT SEVPY AGINIGPVHK KDVMKASVML EHDPQYAVIL AFDVRIERDA QEMADSLGVR IFSAEIIYHL FDAFTKYRQD YKKQK QEEF KHIAVFPCKI KILPQYIFNS RDPIVMGVTV EAGQVKQGTP MCVPSKNFVD IGIVTSIEIN HKQVDVAKKG QEVCVK IEP IPGESPKMFG RHFEATDILV SKISRQSIDA LKDWFRDEMQ KSDWQLIVEL KKVFEI

UniProtKB: Eukaryotic translation initiation factor 5B

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Macromolecule #4: Translation initiation factor eIF1A

MacromoleculeName: Translation initiation factor eIF1A / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 10.384011 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString:
RELVFKEDGQ EYAQVIKMLG NGRLEAMCFD GVKRLCHIRG KLRKKVWINT SDIILVGLRD YQDNKADVIL KYNADEARSL KAYGELPEH A

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Macromolecule #5: ribosomal protein uS2

MacromoleculeName: ribosomal protein uS2 / type: protein_or_peptide / ID: 5 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 47.84359 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: SGALDVLQMK EEDVLKFLAA GTHLGGTNLD FQMEQYIYKR KSDGIYIINL KRTWEKLLLA ARAIVAIENP ADVSVISSRN TGQRAVLKF AAATGATPIA GRFTPGTFTN QIQAAFREPR LLVVTDPRAD HQPLTEASYV NLPTIALCNT DSPLRYVDIA I PCNNKGAH ...String:
SGALDVLQMK EEDVLKFLAA GTHLGGTNLD FQMEQYIYKR KSDGIYIINL KRTWEKLLLA ARAIVAIENP ADVSVISSRN TGQRAVLKF AAATGATPIA GRFTPGTFTN QIQAAFREPR LLVVTDPRAD HQPLTEASYV NLPTIALCNT DSPLRYVDIA I PCNNKGAH SVGLMWWMLA REVLRMRGTI SREHPWEVMP DLYFYRDPVV DPFSKKDWYD VKAPAMFNIR NIGKTLVTRT QG TKIASDG LKGRVFEVSL ADLQNDEVAF RKFKLITEDV QGKNCLTNFH GMDLTRDKMC SMVKKWQTMI EAHVDVKTTD GYL LRLFCV GFTKKRNNQI RKTSYAQHQQ VRQIRKKMME IMTREVQTND LKEVVNKLIP DSIGKDIEKA CQSIYPLHDV FVRK VKMLK KPKFELGKLM ELHG

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Macromolecule #6: 40S ribosomal protein S2

MacromoleculeName: 40S ribosomal protein S2 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 24.091453 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: EWMPVTKLGR LVKDMKIKSL EEIYLFSLPI KESEIIDFFL GASLKDEVLK IMPVQKQTRA GQRTRFKAFV AIGDYNGHVG LGVKCSKEV ATAIRGAIIL AKLSIVPVRR GYWGNKIGKP HTVPCKVTGR CGSVLVRLIP APRGTGIVSA PVPKKLLMMA G IDDCYTSA ...String:
EWMPVTKLGR LVKDMKIKSL EEIYLFSLPI KESEIIDFFL GASLKDEVLK IMPVQKQTRA GQRTRFKAFV AIGDYNGHVG LGVKCSKEV ATAIRGAIIL AKLSIVPVRR GYWGNKIGKP HTVPCKVTGR CGSVLVRLIP APRGTGIVSA PVPKKLLMMA G IDDCYTSA RGCTATLGNF AKATFDAISK TYSYLTPDLW KETVFTKSPY QEFTDHLVKT

UniProtKB: Small ribosomal subunit protein uS5

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Macromolecule #7: 40S ribosomal protein S4, X isoform

MacromoleculeName: 40S ribosomal protein S4, X isoform / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 29.164336 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: ARGPKKHLKR VAAPKHWMLD KLTGVFAPRP STGPHKLREC LPLIIFLRNR LKYALTGDEV KKICMQRFIK IDGKVRTDIT YPAGFMDVI SIDKTGENFR LIYDTKGRFA VHRITPEEAK YKLCKVRKIF VGTKGIPHLV THDARTIRYP DPLIKVNDTI Q IDLETGKI ...String:
ARGPKKHLKR VAAPKHWMLD KLTGVFAPRP STGPHKLREC LPLIIFLRNR LKYALTGDEV KKICMQRFIK IDGKVRTDIT YPAGFMDVI SIDKTGENFR LIYDTKGRFA VHRITPEEAK YKLCKVRKIF VGTKGIPHLV THDARTIRYP DPLIKVNDTI Q IDLETGKI TDFIKFDTGN LCMVTGGANL GRIGVITNRE RHPGSFDVVH VKDANGNSFA TRLSNIFVIG KGNKPWISLP RG KGIRLTI AEERDKRLAA K

UniProtKB: Small ribosomal subunit protein eS4, X isoform

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Macromolecule #8: 40S ribosomal protein S3

MacromoleculeName: 40S ribosomal protein S3 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA-(apurinic or apyrimidinic site) lyase
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 24.970289 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: VQISKKRKFV ADGIFKAELN EFLTRELAED GYSGVEVRVT PTRTEIIILA TRTQNVLGEK GRRIRELTAV VQKRFGFPEG SVELYAEKV ATRGLCAIAQ AESLRYKLLG GLAVRRACYG VLRFIMESGA KGCEVVVSGK LRGQRAKSMK FVDGLMIHSG D PVNYYVDT ...String:
VQISKKRKFV ADGIFKAELN EFLTRELAED GYSGVEVRVT PTRTEIIILA TRTQNVLGEK GRRIRELTAV VQKRFGFPEG SVELYAEKV ATRGLCAIAQ AESLRYKLLG GLAVRRACYG VLRFIMESGA KGCEVVVSGK LRGQRAKSMK FVDGLMIHSG D PVNYYVDT AVRHVLLRQG VLGIKVKIML PWDPTGKIGP KKPLPDHVSI VEPKDEILPT TPISEQK

UniProtKB: Small ribosomal subunit protein uS3

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Macromolecule #9: 40S ribosomal protein S6

MacromoleculeName: 40S ribosomal protein S6 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 26.157934 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MKLNISFPAT GCQKLIEVDD ERKLRTFYEK RMATEVAADA LGEEWKGYVV RISGGNDKQG FPMKQGVLTH GRVRLLLSKG HSCYRPRRT GERKRKSVRG CIVDANLSVL NLVIVKKGEK DIPGLTDTTV PRRLGPKRAS RIRKLFNLSK EDDVRQYVVR K PLNKEGKK ...String:
MKLNISFPAT GCQKLIEVDD ERKLRTFYEK RMATEVAADA LGEEWKGYVV RISGGNDKQG FPMKQGVLTH GRVRLLLSKG HSCYRPRRT GERKRKSVRG CIVDANLSVL NLVIVKKGEK DIPGLTDTTV PRRLGPKRAS RIRKLFNLSK EDDVRQYVVR K PLNKEGKK PRTKAPKIQR LVTPRVLQHK RRRIALKKQR TKKNKEEAAE YAKLLAKRMK EAKEKRQE

UniProtKB: Small ribosomal subunit protein eS6

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Macromolecule #10: 40S ribosomal protein S7

MacromoleculeName: 40S ribosomal protein S7 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 21.187762 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString:
IVKPNGEKPD EFESGISQAL LELEMNSDLK AQLRELNITA AKEIEVGGGR KAIIIFVPVP QLKSFQKIQV RLVRELEKKF SGKHVVFIA QRRILPKPTR KSRTKNKQKR PRSRTLTAVH DAILEDLVFP SEIVGKRIRV KLDGSRLIKV HLDKAQQNNV E HKVETFSG VYKKLTGKDV NFEFPEF

UniProtKB: Small ribosomal subunit protein eS7

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Macromolecule #11: 40S ribosomal protein S8

MacromoleculeName: 40S ribosomal protein S8 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 23.945959 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: GISRDNWHKR RKTGGKRKPY HKKRKYELGR PAANTKIGPR RIHTVRVRGG NKKYRALRLD VGNFSWGSEC CTRKTRIIDV VYNASNNEL VRTKTLVKNC IVLIDSTPYR QWYESHYALP LGRKKGAKLT PEEEEILNKK RSKKIQKKYD ERKKNAKISS L LEEQFQQG ...String:
GISRDNWHKR RKTGGKRKPY HKKRKYELGR PAANTKIGPR RIHTVRVRGG NKKYRALRLD VGNFSWGSEC CTRKTRIIDV VYNASNNEL VRTKTLVKNC IVLIDSTPYR QWYESHYALP LGRKKGAKLT PEEEEILNKK RSKKIQKKYD ERKKNAKISS L LEEQFQQG KLLACIASRP GQCGRADGYV LEGKELEFYL RKIKARK

UniProtKB: Small ribosomal subunit protein eS8

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Macromolecule #12: 40S ribosomal protein S9

MacromoleculeName: 40S ribosomal protein S9 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 21.279271 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString:
PVARSWVCRK TYVTPRRPFE KSRLDQELKL IGEYGLRNKR EVWRVKFTLA KIRKAARELL TLDEKDPRRL FEGNALLRRL VRIGVLDEG KMKLDYILGL KIEDFLERRL QTQVFKLGLA KSIHHARVLI RQRHIRVRKQ VVNIPSFIVR LDSQKHIDFS L RSPYGGGR PGRVKRKNAK KG

UniProtKB: Small ribosomal subunit protein uS4

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Macromolecule #13: 40S ribosomal protein S5

MacromoleculeName: 40S ribosomal protein S5 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 21.327723 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString:
DIKLFGKWST DDVQINDISL QDYIAVKEKY AKYLPHSAGR YAAKRFRKAQ CPIVERLTNS MMMHGRNNGK KLMTVRIVKH AFEIIHLLT GENPLQVLVN AIINSGPRED STRIGRAGTV RRQAVDVSPL RRVNQAIWLL CTGAREAAFR NIKTIAECLA D ELINAAKG SSNSYAIKKK DELERVAKSN R

UniProtKB: Small ribosomal subunit protein uS7

+
Macromolecule #14: 40S ribosomal protein S11

MacromoleculeName: 40S ribosomal protein S11 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 17.170248 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString:
ADIQTERAYQ KQPTIFQNKK RVLLGETGKE KLPRYYKNIG LGFKTPKEAI EGTYIDKKCP FTGNVSIRGR ILSGVVTKMK MQRTIVIRR DYLHYIRKYN RFEKRHKNMS VHLSPCFRDV QIGDIVTVGE CRPLSKTVRF NVLKVTKA

UniProtKB: Small ribosomal subunit protein uS17

+
Macromolecule #15: 40S ribosomal protein S10

MacromoleculeName: 40S ribosomal protein S10 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 11.61676 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString:
MLMPKKNRIA IYELLFKEGV MVAKKDVHMP KHPELADKNV PNLHVMKAMQ SLKSRGYVKE QFAWRHFYWY LTNEGIQYLR DYLHLPPEI VPATLRRS

UniProtKB: Small ribosomal subunit protein eS10

+
Macromolecule #16: ribosomal protein uS15

MacromoleculeName: ribosomal protein uS15 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 16.340327 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString:
GRMHAPGKGL SQSALPYRRS VPTWLKLTSD DVKEQIYKLA KKGLTPSQIG VILRDSHGVA QVRFVTGNKI LRILKSKGLA PDLPEDLYH LIKKAVAVRK HLERNRKDKD AKFRLILIES RIHRLARYYK TKRVLPPNWK YE

+
Macromolecule #17: ribosomal protein eS12

MacromoleculeName: ribosomal protein eS12 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 13.347567 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString:
GVMDVNTALQ EVLKTALIHD GLARGIREAA KALDKRQAHL CVLASNCDEP MYVKLVEALC AEHQINLIKV DDNKKLGEWV GLCKIDREG KPRKVVGCSC VVVKDYGKES QAKDVIEEYF K

+
Macromolecule #18: ribosomal protein uS11

MacromoleculeName: ribosomal protein uS11 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 14.344424 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString:
GPQVAEGENV FGVCHIFASF NDTFVHVTDL SGKETICRVT GGMKVKADRD ESSPYAAMLA AQDVAQRCKE LGITALHIKL RATGGNRTK TPGPGAQSAL RALARSGMKI GRIEDVTPIP SDSTRRKGGR RGRRL

+
Macromolecule #19: 40S ribosomal protein S15

MacromoleculeName: 40S ribosomal protein S15 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 13.586022 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString:
FTYRGVDLDQ LLDMSYEQLM QLYSARQRRR LNRGLRRKQH SLLKRLRKAK KEAPPMEKPE VVKTHLRDMI ILPEMVGSMV GVYNGKTFN QVEIKPEMIG HYLGEFSITY KPVKHG

UniProtKB: Small ribosomal subunit protein uS19

+
Macromolecule #20: 40S ribosomal protein S16

MacromoleculeName: 40S ribosomal protein S16 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 15.76548 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString:
QSVQVFGRKK TATAVAHCKR GNGLIKVNGR PLEMIEPRTL QYKLLEPVLL LGKERFAGVD IRVRVKGGGH VAQIYAIRQS ISKALVAYY QKYVDEASKK EIKDILIQYD RTLLVADPRR CESKKFGGPG ARARYQKSYR

UniProtKB: Small ribosomal subunit protein uS9

+
Macromolecule #21: ribosomal protein eS17

MacromoleculeName: ribosomal protein eS17 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 15.250714 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString:
GRVRTKTVKK AARVIIEKYY TRLGNDFHTN KRVCEEIAII PSKKLRNKIA GYVTHLMKRI QRGPVRGISI KLQEEERERR DNYVPEVSA LDQEIIEVDP DTKEMLKLLD FGSLSNLQVT QPTVGMNFKT PRG

+
Macromolecule #22: ribosomal protein uS13

MacromoleculeName: ribosomal protein uS13 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 16.496223 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString:
LVIPEKFQHI LRVLNTNIDG RRKIAFAITA IKGVGRRYAH VVLRKADIDL TKRAGELTED EVERVITIMQ NPRQYKIPDW FLNRQKDVK DGKYSQVLAN GLDNKLREDL ERLKKIRAHR GLRHFWGLRV RGQHTKTTGR R

+
Macromolecule #23: 40S ribosomal protein S19

MacromoleculeName: 40S ribosomal protein S19 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 15.409689 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString:
VTVKDVNQQE FVRALAAFLK KSGKLKVPEW VDTVKLAKHK ELAPYDENWF YTRAASTARH LYLRGGAGVG SMTKIYGGRQ RNGVMPSHF SRGSKSVARR VLQALEGLKM VEKDQDGGRK LTPQGQRDLD RIAGQVAAAN

UniProtKB: Small ribosomal subunit protein eS19

+
Macromolecule #24: ribosomal protein uS10

MacromoleculeName: ribosomal protein uS10 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 11.508648 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString:
IHRIRITLTS RNVKSLEKVC ADLIRGAKEK NLKVKGPVRM PTKTLRITTR KTPCGEGSKT WDRFQMRIHK RLIDLHSPSE IVKQITSIS IEPGVEVEVT IA

+
Macromolecule #25: 40S ribosomal protein S15a

MacromoleculeName: 40S ribosomal protein S15a / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 14.734357 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString:
VRMNVLADAL KSINNAEKRG KRQVLIRPCS KVIVRFLTVM MKHGYIGEFE IIDDHRAGKI VVNLTGRLNK CGVISPRFDV QLKDLEKWQ NNLLPSRQFG FIVLTTSAGI MDHEEARRKH TGGKILGFFF

UniProtKB: Small ribosomal subunit protein uS8

+
Macromolecule #26: 40S ribosomal protein S23

MacromoleculeName: 40S ribosomal protein S23 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 15.626392 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString:
GKCRGLRTAR KLRSHRRDQK WHDKQYKKAH LGTALKANPF GGASHAKGIV LEKVGVEAKQ PNSAIRKCVR VQLIKNGKKI TAFVPNDGC LNFIEENDEV LVAGFGRKGH AVGDIPGVRF KVVKVANVSL LALYKGKKER PR

UniProtKB: Small ribosomal subunit protein uS12

+
Macromolecule #27: Isoform 3 of 40S ribosomal protein S24

MacromoleculeName: Isoform 3 of 40S ribosomal protein S24 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 14.361046 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString:
TVTIRTRKFM TNRLLQRKQM VIDVLHPGKA TVPKTEIREK LAKMYKTTPD VIFVFGFRTH FGGGKTTGFG MIYDSLDYAK KNEPKHRLA RHGLYEKKKT SRKQRKERKN RMKKVRGTAK ANVG

UniProtKB: Small ribosomal subunit protein eS24

+
Macromolecule #28: 40S ribosomal protein S21

MacromoleculeName: 40S ribosomal protein S21 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 8.977214 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString:
MQNDAGEFVD LYVPRKCSAS NRIIGAKDHA SIQMNVAEVD KVTGRFNGQF KTYAICGAIR RMGESDDSIL RLAKADGIVS KN

UniProtKB: Small ribosomal subunit protein eS21

+
Macromolecule #29: ribosomal protein eS25

MacromoleculeName: ribosomal protein eS25 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 8.182693 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString:
DKLNNLVLFD KATYDKLCKE VPNYKLITPA VVSERLKIRG SLARAALQEL LSKGLIKLVS KHRAQVIYTR NT

+
Macromolecule #30: 40S ribosomal protein S27

MacromoleculeName: 40S ribosomal protein S27 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 9.210843 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString:
PLAKDLLHPS PEEEKRKHKK KRLVQSPNSY FMDVKCPGCY KITTVFSHAQ TVVLCVGCST VLCQPTGGKA RLTEGCSFRR KQ

UniProtKB: Small ribosomal subunit protein eS27

+
Macromolecule #31: 40S ribosomal protein S26

MacromoleculeName: 40S ribosomal protein S26 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 11.341426 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString:
TKKRRNNGRA KKGRGHVQPI RCTNCARCVP KDKAIKKFVI RNIVEAAAVR DISEASVFDA YVLPKLYVKL HYCVSCAIHS KVVRNRSRE ARKDRTPPPR

UniProtKB: Small ribosomal subunit protein eS26

+
Macromolecule #32: ribosomal protein eS28

MacromoleculeName: ribosomal protein eS28 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 6.451396 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString:
PIKLARVTKV LGRTGSQGQC TQVRVEFMDD TSRSIIRNVK GPVREGDVLT LLESEREA

+
Macromolecule #33: ribosomal protein eS30

MacromoleculeName: ribosomal protein eS30 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 5.640648 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString:
AAAAAARAGK VRGQTPKVAK QEKKKKKTGR AKRRMQYNRR FVNVKGPNAN S

+
Macromolecule #34: ribosomal protein uS14

MacromoleculeName: ribosomal protein uS14 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 6.502573 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString:
HQQLYWSHPR KFGQGSRSCR VCSNRHGLIR KYGLNMCRQC FRQYAKDIGF IKLD

+
Macromolecule #35: 40S ribosomal protein S27a

MacromoleculeName: 40S ribosomal protein S27a / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 7.623009 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString:
YTTPKKNKHK RKKVKLAVLK YYKVDENGKI SRLRRECPSD ECGAGVFMAS HFDRHYCGKC CLTYC

UniProtKB: Ubiquitin-ribosomal protein eS31 fusion protein

+
Macromolecule #36: ribosomal protein eL41

MacromoleculeName: ribosomal protein eL41 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 2.683481 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString:
MRAKWRKKRM RRLKRKRRK

+
Macromolecule #37: Receptor of activated protein C kinase 1

MacromoleculeName: Receptor of activated protein C kinase 1 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 34.726164 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: TEQMTLRGTL KGHNGWVTQI ATTPQFPDMI LSASRDKTII MWKLTRDETN YGIPQRALRG HSHFVSDVVI SSDGQFALSG SWDGTLRLW DLTTGTTTRR FVGHTKDVLS VAFSSDNRQI VSGSRDKTIK LWNTLGVCKY TVQDESHSEW VSCVRFSPNS S NPIIVSCG ...String:
TEQMTLRGTL KGHNGWVTQI ATTPQFPDMI LSASRDKTII MWKLTRDETN YGIPQRALRG HSHFVSDVVI SSDGQFALSG SWDGTLRLW DLTTGTTTRR FVGHTKDVLS VAFSSDNRQI VSGSRDKTIK LWNTLGVCKY TVQDESHSEW VSCVRFSPNS S NPIIVSCG WDKLVKVWNL ANCKLKTNHI GHTGYLNTVT VSPDGSLCAS GGKDGQAMLW DLNEGKHLYT LDGGDIINAL CF SPNRYWL CAATGPSIKI WDLEGKIIVD ELKQEVISTS SKAEPPQCTS LAWSADGQTL FAGYTDNLVR VWQVTIG

UniProtKB: Small ribosomal subunit protein RACK1

+
Macromolecule #2: 18S ribosomal RNA

MacromoleculeName: 18S ribosomal RNA / type: rna / ID: 2 / Number of copies: 1
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 534.54475 KDa
SequenceString: UACCUGGUUG AUCCUGCCAG UAGCAUAUGC UUGUCUCAAA GAUUAAGCCA UGCAUGUCUA AGUACGCACG GCCGGUACAG UGAAACUGC GAAUGGCUCA UUAAAUCAGU UAUGGUUCCU UUGGUCGCUC GUACUUGGAU AACUGUGGUA AUUCUAGAGC U AAUACAUG ...String:
UACCUGGUUG AUCCUGCCAG UAGCAUAUGC UUGUCUCAAA GAUUAAGCCA UGCAUGUCUA AGUACGCACG GCCGGUACAG UGAAACUGC GAAUGGCUCA UUAAAUCAGU UAUGGUUCCU UUGGUCGCUC GUACUUGGAU AACUGUGGUA AUUCUAGAGC U AAUACAUG CCGACGGGCG CUGACCCCCU UCGCGGGGGG GAUGCGUGCA UUUAUCAGUG GUGACUCUAG AUAACCUCGG GC CGAUCGC ACGCCGGCGG CGACGACCCA UUCGAACGUC UGCCCUAUCA ACUUUCGAUG GUAGUCGCCG UGCCUACCAU GGU GACCAC GGGUGACGGG GAAUCAGGGU UCGAUUCCGG AGAGGGAGCC UGAGAAACGG CUACCACAUC CAAGGAAGGC AGCA GGCGC GCAAAUUACC CACUCCCGAC CCGGGGAGGU AGUGACGAAA AAUAACAAUA CAGGACUCUU UCGAGGCCCU GUAAU UGGA AUGAGUCCAC UUUAAAUCCU UUAACGAGGA UCCAUUGGAG GGCAAGUCUG GUGCCAGCAG CCGCGGUAAU UCCAGC UCC AAUAGCGUAU AUUAAAGUUG CUGCAGUUAA AAAGCUCGUA GUUGGAUCUC UAGCGUUUAC UUUGAAAAAA UUAGAGU GU UCAAAGCAGG CCGCCUGGAU ACCGCAGCUA GGAAUAAUGG AAUAGGACCG CGGUUCUAUU UUGUUGGUUU UCGGAACU G AGGCCAUGAU UAAGAGGGAC GGCCGGGGGC AUUCGUAUUG CGCCGCUAGA GGUGAAAUUC UUGGACCGGC GCAAGACGG ACCAGAGCGA AAGCAUUUGC CAAGAAUGUU UUCAUUAAUC AAGAACGAAA GUCGGAGGUU CGAAGACGAU CAGAUACCGU CGUAGUUCC GACCAUAAAC GAUGCCGACC GGCGAUGCGG CGGCGUUAUU CCCAUGACCC GCCGGGCAGC UUCCGGGAAA C CAAAGUCU UUGGGUUCCG GGGGGAGUAU GGUUGCAAAG CUGAAACUUA AAGGAAUUGA CGGAAGGGCA CCACCAGGAG UG GAGCCUG CGGCUUAAUU UGACUCAACA CGGGAAACCU CACCCGGCCC GGACACGGAC AGGAUUGACA GAUUGAUAGC UCU UUCUCG AUUCCGUGGG UGGUGGUGCA UGGCCGUUCU UAGUUGGUGG AGCGAUUUGU CUGGUUAAUU CCGAUAACGA ACGA GACUC UGGCAUGCUA ACUAGUUACG CGACCGGUCG GCGUAACUUC UUAGAGGGAC AAGUGGCGUU CAGCCACCCG AGAUU GAGC AAUAACAGGU CUGUGAUGCC CUUAGAUGUC CGGGGCUGCA CGCGCGCUAC ACUGACUGGC UCAGCGUGUC CUACGC CGG CAGGCGCGGG UAACCCGUUG AACCCCAUUC GUGAUGGGGA UCGGGGAUUG CAAUUAUUCC CCAUGAACGA GGAAUUC CC AGUAAGUGCG GGUCAUAAGC UUGCGUUGAU UAAGUCCCUG CCCUUUGUAC ACACCGCCCG UCGCUACUAC CGAUUGGA U GGUUUAGUGA GGCCCUCGGA UCGGCCCCGC CGGGGUGCCC UGGCGGAGCG CUGAGAAGAC GGUCGAACUU GACUAUCUA GAGGAAGUAA AAGUCGUAAC AAGGUUUCCG UAGGUGAACC UGCGGAAGGA UCAUUA

+
Macromolecule #3: Human Met-tRNAiMet

MacromoleculeName: Human Met-tRNAiMet / type: rna / ID: 3 / Number of copies: 1
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 24.23151 KDa
SequenceString:
AGCAGAGUGG CGCAGCGGAA GCGUGCUGGG CCCAUAACCC AGAGGUCGAU GGAUCGAAAC CAUCCUCUGC UACCA

GENBANK: GENBANK: K00328.1

+
Macromolecule #38: PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER

MacromoleculeName: PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER / type: ligand / ID: 38 / Number of copies: 1 / Formula: GNP
Molecular weightTheoretical: 522.196 Da
Chemical component information

ChemComp-GNP:
PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER / GppNHp, GMPPNP, energy-carrying molecule analogue*YM

+
Macromolecule #39: GUANOSINE-5'-MONOPHOSPHATE

MacromoleculeName: GUANOSINE-5'-MONOPHOSPHATE / type: ligand / ID: 39 / Number of copies: 1 / Formula: 5GP
Molecular weightTheoretical: 363.221 Da
Chemical component information

ChemComp-5GP:
GUANOSINE-5'-MONOPHOSPHATE

+
Macromolecule #40: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 40 / Number of copies: 3 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

-
Experimental details

-
Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

-
Sample preparation

BufferpH: 7.2
GridModel: Quantifoil R2/2 / Material: COPPER / Mesh: 300
VitrificationCryogen name: ETHANE

-
Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 70.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 50.0 µm / Illumination mode: OTHER / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 2.0 µm / Nominal defocus min: 0.5 µm
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

-
Image processing

Startup modelType of model: INSILICO MODEL
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.2 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 190000
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: PROJECTION MATCHING

+
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