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- EMDB-26004: Cryo-EM map of the full-length relaxin receptor RXFP1 in complex ... -
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Open data
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Basic information
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Title | Cryo-EM map of the full-length relaxin receptor RXFP1 in complex with heterotrimeric Gs | |||||||||
![]() | Relaxin receptor RXFP1 in complex with heterotrimeric Gs | |||||||||
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Function / homology | ![]() lung connective tissue development / nipple morphogenesis / Relaxin receptors / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | |||||||||
Biological species | ![]() ![]() ![]() ![]() ![]() | |||||||||
Method | ![]() ![]() | |||||||||
![]() | Erlandson SC / Rawson S / Kruse AC | |||||||||
Funding support | ![]()
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![]() | ![]() Title: The relaxin receptor RXFP1 signals through a mechanism of autoinhibition. Authors: Sarah C Erlandson / Shaun Rawson / James Osei-Owusu / Kelly P Brock / Xinyue Liu / Joao A Paulo / Julian Mintseris / Steven P Gygi / Debora S Marks / Xiaojing Cong / Andrew C Kruse / ![]() ![]() Abstract: The relaxin family peptide receptor 1 (RXFP1) is the receptor for relaxin-2, an important regulator of reproductive and cardiovascular physiology. RXFP1 is a multi-domain G protein-coupled receptor ...The relaxin family peptide receptor 1 (RXFP1) is the receptor for relaxin-2, an important regulator of reproductive and cardiovascular physiology. RXFP1 is a multi-domain G protein-coupled receptor (GPCR) with an ectodomain consisting of a low-density lipoprotein receptor class A (LDLa) module and leucine-rich repeats. The mechanism of RXFP1 signal transduction is clearly distinct from that of other GPCRs, but remains very poorly understood. In the present study, we determine the cryo-electron microscopy structure of active-state human RXFP1, bound to a single-chain version of the endogenous agonist relaxin-2 and the heterotrimeric G protein. Evolutionary coupling analysis and structure-guided functional experiments reveal that RXFP1 signals through a mechanism of autoinhibition. Our results explain how an unusual GPCR family functions, providing a path to rational drug development targeting the relaxin receptors. | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 55.9 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 23.3 KB 23.3 KB | Display Display | ![]() |
Images | ![]() | 35.3 KB | ||
Others | ![]() ![]() ![]() | 71.3 MB 71.4 MB 71.5 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7tmwC C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
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Annotation | Relaxin receptor RXFP1 in complex with heterotrimeric Gs | ||||||||||||||||||||
Voxel size | X=Y=Z: 1.06 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Additional map: Relaxin receptor RXFP1 in complex with heterotrimeric Gs
File | emd_26004_additional_1.map | ||||||||||||
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Annotation | Relaxin receptor RXFP1 in complex with heterotrimeric Gs | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: Relaxin receptor RXFP1 in complex with heterotrimeric Gs
File | emd_26004_half_map_1.map | ||||||||||||
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Annotation | Relaxin receptor RXFP1 in complex with heterotrimeric Gs | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: Relaxin receptor RXFP1 in complex with heterotrimeric Gs
File | emd_26004_half_map_2.map | ||||||||||||
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Annotation | Relaxin receptor RXFP1 in complex with heterotrimeric Gs | ||||||||||||
Projections & Slices |
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Density Histograms |
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Sample components
-Entire : Complex of the relaxin receptor RXFP1 and heterotrimeric Gs
Entire | Name: Complex of the relaxin receptor RXFP1 and heterotrimeric Gs |
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Components |
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-Supramolecule #1: Complex of the relaxin receptor RXFP1 and heterotrimeric Gs
Supramolecule | Name: Complex of the relaxin receptor RXFP1 and heterotrimeric Gs type: complex / ID: 1 / Parent: 0 / Macromolecule list: all |
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Molecular weight | Theoretical: 180 KDa |
-Supramolecule #2: Relaxin receptor 1, Guanine nucleotide-binding protein G(s) subun...
Supramolecule | Name: Relaxin receptor 1, Guanine nucleotide-binding protein G(s) subunit alpha isoforms short type: complex / ID: 2 / Parent: 1 / Macromolecule list: #1 |
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Source (natural) | Organism: ![]() ![]() |
-Supramolecule #3: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1,...
Supramolecule | Name: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1, Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 type: complex / ID: 3 / Parent: 1 / Macromolecule list: #2-#3 |
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Source (natural) | Organism: ![]() ![]() |
-Supramolecule #4: Camelid antibody VHH fragment Nb35
Supramolecule | Name: Camelid antibody VHH fragment Nb35 / type: complex / ID: 4 / Parent: 1 / Macromolecule list: #4 |
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Source (natural) | Organism: ![]() ![]() ![]() |
-Macromolecule #1: Relaxin receptor 1, Guanine nucleotide-binding protein G(s) subun...
Macromolecule | Name: Relaxin receptor 1, Guanine nucleotide-binding protein G(s) subunit alpha isoforms short fusion type: protein_or_peptide / ID: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() ![]() |
Recombinant expression | Organism: ![]() ![]() |
Sequence | String: DYKDDDDGGS LEVLFQGPGG SQDVKCSLGY FPCGNITKCL PQLLHCNGVD DCGNQADEDN CGDNNGWSLQ FDKYFASYYK MTSQYPFEAE TPECLVGSVP VQCLCQGLEL DCDETNLRAV PSVSSNVTAM SLQWNLIRKL PPDCFKNYHD LQKLYLQNNK ITSISIYAFR ...String: DYKDDDDGGS LEVLFQGPGG SQDVKCSLGY FPCGNITKCL PQLLHCNGVD DCGNQADEDN CGDNNGWSLQ FDKYFASYYK MTSQYPFEAE TPECLVGSVP VQCLCQGLEL DCDETNLRAV PSVSSNVTAM SLQWNLIRKL PPDCFKNYHD LQKLYLQNNK ITSISIYAFR GLNSLTKLYL SHNRITFLKP GVFEDLHRLE WLIIEDNHLS RISPPTFYGL NSLILLVLMN NVLTRLPDKP LCQHMPRLHW LDLEGNHIHN LRNLTFISCS NLTVLVMRKN KINHLNENTF APLQKLDELD LGSNKIENLP PLIFKDLKEL SQLNLSYNPI QKIQANQFDY LVKLKSLSLE GIEISNIQQR MFRPLMNLSH IYFKKFQYCG YAPHVRSCKP NTDGISSLEN LLASIIQRVF VWVVSAVTCF GNIFVICMRP YIRSENKLYA MSIISLCCAD CLMGIYLFVI GGFDLKFRGE YNKHAQLWME STHCQLVGSL AILSTEVSVL LLTFLTLEKY ICIVYPFRCV RPGKCRTITV LILIWITGFI VAFIPLSNKE FFKNYYGTNG VCFPLHSEDT ESIGAQIYSV AIFLGINLAA FIIIVFSYGS MFYSVHQSAI TATEIRNQVK KEMILAKRFF FIVFTDALCW IPIFVVKFLS LLQVEIPGTI TSWVVIFILP INSALNPILY TLTTRPFKEM IHRFWYNYRQ RKSMDSKGQK TYAPSFIWVE MWPLQEMPPE LMKPDLNSKT EDQRNEEKAQ REANKKIEKQ LQKDKQVYRA THRLLLLGAD NSGKSTIVKQ MRIYHGGSGG SGGTSGIFET KFQVDKVNFH MFDVGGQRDE RRKWIQCFND VTAIIFVVDS SDYNRLQEAL NLFKSIWNNR WLRTISVILF LNKQDLLAEK VLAGKSKIED YFPEFARYTT PEDATPEPGE DPRVTRAKYF IRDEFLRIST ASGDGRHYCY PHFTCAVDTE NARRIFNDCR DIIQRMHLRQ YELL UniProtKB: Relaxin receptor 1, Guanine nucleotide-binding protein G(s) subunit alpha isoforms short |
-Macromolecule #2: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
Macromolecule | Name: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 type: protein_or_peptide / ID: 2 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() ![]() |
Recombinant expression | Organism: ![]() ![]() ![]() |
Sequence | String: MHHHHHHGSS GSELDQLRQE AEQLKNQIRD ARKACADATL SQITNNIDPV GRIQMRTRRT LRGHLAKIYA MHWGTDSRLL VSASQDGKLI IWDSYTTNKV HAIPLRSSWV MTCAYAPSGN YVACGGLDNI CSIYNLKTRE GNVRVSRELA GHTGYLSCCR FLDDNQIVTS ...String: MHHHHHHGSS GSELDQLRQE AEQLKNQIRD ARKACADATL SQITNNIDPV GRIQMRTRRT LRGHLAKIYA MHWGTDSRLL VSASQDGKLI IWDSYTTNKV HAIPLRSSWV MTCAYAPSGN YVACGGLDNI CSIYNLKTRE GNVRVSRELA GHTGYLSCCR FLDDNQIVTS SGDTTCALWD IETGQQTTTF TGHTGDVMSL SLAPDTRLFV SGACDASAKL WDVREGMCRQ TFTGHESDIN AICFFPNGNA FATGSDDATC RLFDLRADQE LMTYSHDNII CGITSVSFSK SGRLLLAGYD DFNCNVWDAL KADRAGVLAG HDNRVSCLGV TDDGMAVATG SWDSFLKIWN UniProtKB: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 |
-Macromolecule #3: Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2
Macromolecule | Name: Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 type: protein_or_peptide / ID: 3 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() ![]() |
Recombinant expression | Organism: ![]() ![]() ![]() |
Sequence | String: MASNNTASIA QARKLVEQLK MEANIDRIKV SKAAADLMAY CEAHAKEDPL LTPVPASENP FREKKFFCAI L UniProtKB: Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 |
-Macromolecule #4: Camelid antibody VHH fragment Nb35
Macromolecule | Name: Camelid antibody VHH fragment Nb35 / type: protein_or_peptide / ID: 4 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() ![]() ![]() |
Recombinant expression | Organism: ![]() ![]() ![]() |
Sequence | String: QVQLQESGGG LVQPGGSLRL SCAASGFTFS NYKMNWVRQA PGKGLEWVSD ISQSGASISY TGSVKGRFTI SRDNAKNTLY LQMNSLKPED TAVYYCARCP APFTRDCFDV TSTTYAYRGQ GTQVTVSSLE VLFQGPGHHH HHHHHGSEDQ VDPRLIDGK |
-Experimental details
-Structure determination
Method | ![]() |
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Aggregation state | particle |
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Sample preparation
Concentration | 0.2 mg/mL |
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Buffer | pH: 7.5 |
Vitrification | Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 283.15 K / Instrument: FEI VITROBOT MARK IV |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD![]() |
Sample stage | Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER |
Image recording | Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 52.0 e/Å2 |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
Particle selection | Number selected: 15826542 |
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Startup model | Type of model: OTHER / Details: Ab initio model generated in RELION |
Initial angle assignment | Type: RANDOM ASSIGNMENT / Software - Name: RELION (ver. 3.1) |
Final angle assignment | Type: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3.1) |
Final reconstruction | Applied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 4.2 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 3.1) / Number images used: 45146 |