EMDB-25441: Structure of Bfr2-Lcp5 Complex Observed in the Small Subunit Proc...

Basic information

Entry

Database: EMDB / ID: EMD-25441

• Title: Structure of Bfr2-Lcp5 Complex Observed in the Small Subunit Processome Isolated from R2TP-depleted Yeast Cells
• Map data: Composite map

Sample

• Complex: Structure of Bfr2-Lcp5 Complex Observed in the Small Subunit Processome Isolated from R2TP-depleted Yeast Cells
  • Protein or peptide: x 63 types
  • RNA: x 3 types

• Keywords: Bfr2 / Lcp5 / Small subunit processome / RIBOSOMAL PROTEIN / RIBOSOME

Function / homology

Function:

rRNA acetylation involved in maturation of SSU-rRNA / 18S rRNA cytidine N-acetyltransferase activity / box H/ACA snoRNA binding / tRNA N4-acetyltransferase activity / regulation of ribosomal protein gene transcription by RNA polymerase II / rRNA small subunit pseudouridine methyltransferase Nep1 / tRNA acetylation / Noc4p-Nop14p complex / RNA fragment catabolic process / rRNA 2'-O-methylation / CURI complex / UTP-C complex / t-UTP complex / endonucleolytic cleavage in ITS1 upstream of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / nuclear microtubule / Mpp10 complex / snoRNA guided rRNA 2'-O-methylation / Pwp2p-containing subcomplex of 90S preribosome / rRNA (pseudouridine) methyltransferase activity / regulation of rRNA processing / box C/D sno(s)RNA binding / septum digestion after cytokinesis / endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / box C/D sno(s)RNA 3'-end processing / rDNA heterochromatin / rRNA methyltransferase activity / endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / regulation of transcription by RNA polymerase I / box C/D methylation guide snoRNP complex / single-stranded telomeric DNA binding / tRNA export from nucleus / rRNA primary transcript binding / rRNA base methylation / sno(s)RNA-containing ribonucleoprotein complex / 90S preribosome assembly / O-methyltransferase activity / small nuclear ribonucleoprotein complex / rRNA methylation / histone H2A Q104 methyltransferase activity / mTORC1-mediated signalling / Protein hydroxylation / mRNA modification / U3 snoRNA binding / poly(U) RNA binding / positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay / Formation of the ternary complex, and subsequently, the 43S complex / poly(A)+ mRNA export from nucleus / Translation initiation complex formation / preribosome, small subunit precursor / Ribosomal scanning and start codon recognition / snoRNA binding / establishment of cell polarity / Major pathway of rRNA processing in the nucleolus and cytosol / positive regulation of transcription by RNA polymerase I / nucleolar large rRNA transcription by RNA polymerase I / SRP-dependent cotranslational protein targeting to membrane / GTP hydrolysis and joining of the 60S ribosomal subunit / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / Formation of a pool of free 40S subunits / L13a-mediated translational silencing of Ceruloplasmin expression / proteasome assembly / 90S preribosome / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / RNA processing / regulation of translational fidelity / ribosomal subunit export from nucleus / ribosomal small subunit export from nucleus / vesicle-mediated transport / Transferases; Acyltransferases; Transferring groups other than aminoacyl groups / RNA endonuclease activity / enzyme activator activity / nuclear periphery / Transferases; Transferring one-carbon groups; Methyltransferases / ribosome assembly / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of SSU-rRNA / small-subunit processome / translational initiation / maintenance of translational fidelity / rRNA processing / unfolded protein binding / protein transport / ribosome biogenesis / ribosomal small subunit assembly / ribosomal small subunit biogenesis / small ribosomal subunit rRNA binding / cytosolic small ribosomal subunit / cytoplasmic translation / tRNA binding / rRNA binding / ribosome / structural constituent of ribosome / translation / ribonucleoprotein complex / mRNA binding / nucleolus / mitochondrion / RNA binding

Domain/homology:

Regulator of rDNA transcription 14 / Regular of rDNA transcription protein 14 / Apoptosis-antagonizing transcription factor, C-terminal / AATF leucine zipper-containing domain / Protein AATF/Bfr2 / : / Apoptosis-antagonizing transcription factor, C-terminal / Apoptosis antagonizing transcription factor / NUC153 / Nucleolar protein 10/Enp2 / NUC153 domain / U3 small nucleolar RNA-associated protein 8 / : / Utp8, N-terminal beta propeller / Utp8, C-terminal / rRNA biogenesis protein Rrp5 / : / : / Ribosomal RNA assembly KRR1 / : / : / : / KRR1 small subunit processome component, second KH domain / U3 small nucleolar RNA-associated protein 20, N-terminal / U3 snoRNA associated / Nucleolar complex protein 4 / U3 small nucleolar RNA-associated protein 20, C-terminal / : / U3 small nucleolar RNA-associated protein 20, N-terminal / U3 snoRNA associated / U3 small nucleolar RNA-associated protein 20 domain / Possible tRNA binding domain / RNA cytidine acetyltransferase NAT10 / Possible tRNA binding domain / Helicase domain / tRNA(Met) cytidine acetyltransferase TmcA, N-terminal / TmcA/NAT10/Kre33 / Helicase / tRNA(Met) cytidine acetyltransferase TmcA, N-terminal / GNAT acetyltransferase 2 / NOL6/Upt22 / Small-subunit processome, Utp11 / Sof1-like protein / BING4, C-terminal domain / Fcf2 pre-rRNA processing, C-terminal / Nrap protein domain 1 / Nrap protein, domain 2 / Nrap protein, domain 3 / Nrap protein, domain 4 / Nrap protein, domain 5 / Nrap protein, domain 6 / rRNA-processing protein Fcf1, PIN domain / Fcf2/DNTTIP2 / WD repeat-containing protein WDR46/Utp7 / : / : / Nrap protein domain 1 / Utp11 protein / Sof1-like domain / BING4CT (NUC141) domain / Fcf2 pre-rRNA processing / Nrap protein PAP/OAS-like domain / Nrap protein domain 3 / Nrap protein nucleotidyltransferase domain 4 / Nrap protein PAP/OAS1-like domain 5 / Nrap protein domain 6 / BING4CT (NUC141) domain / rRNA-processing protein Fcf1/Utp23 / Ribosomal RNA-processing protein 7, C-terminal domain / Ribosomal RNA-processing protein 7 / Rrp7, RRM-like N-terminal domain / Fcf1 / Ribosomal RNA-processing protein 7 (RRP7) C-terminal domain / Rrp7 RRM-like N-terminal domain / U3 small nucleolar RNA-associated protein 15, C-terminal / UTP15 C terminal / Nucleolar protein 58/56, N-terminal / U3 small nucleolar RNA-associated protein 18 / NOP5NT (NUC127) domain / RNA 3'-terminal phosphate cyclase-like, conserved site / RNA 3'-terminal phosphate cyclase signature. / U3 small nucleolar RNA-associated protein 13, C-terminal / Periodic tryptophan protein 2 / Utp13 specific WD40 associated domain / Small-subunit processome, Utp12 / Dip2/Utp12 Family / Sas10/Utp3/C1D / Small-subunit processome, Utp21 / U3 small nucleolar ribonucleoprotein complex, subunit Mpp10 / RNA 3'-terminal phosphate cyclase type 2 / Sas10/Utp3/C1D family / Mpp10 protein / Utp21 specific WD40 associated putative domain / Ribosomal RNA-processing protein Rrp9-like / RNA 3'-terminal phosphate cyclase / Ribosomal biogenesis, methyltransferase, EMG1/NEP1 / RNA 3'-terminal phosphate cyclase, insert domain / RNA 3'-terminal phosphate cyclase domain / RNA 3'-terminal phosphate cyclase, insert domain superfamily / RNA 3'-terminal phosphate cyclase domain superfamily

Component:

Ribonucloprotein / RRP9 isoform 1 / Nucleolar protein 58 / U3 small nucleolar RNA-associated protein 11 / Small ribosomal subunit protein uS4A / Small ribosomal subunit protein uS15 / Small ribosomal subunit protein uS8A / Small ribosomal subunit protein uS12A / Small ribosomal subunit protein eS24A / Small ribosomal subunit protein eS4A / Small ribosomal subunit protein eS6A / Small ribosomal subunit protein eS8A / Small ribosomal subunit protein uS17A / Small ribosomal subunit protein uS9A / rRNA 2'-O-methyltransferase fibrillarin / Ribosomal RNA-processing protein 7 / KRR1 small subunit processome component / Periodic tryptophan protein 2 / Small ribosomal subunit protein uS7 / Small ribosomal subunit protein eS7A / U3 small nucleolar ribonucleoprotein protein IMP3 / Protein SOF1 / U3 small nucleolar RNA-associated protein 20 / Ribosome biogenesis protein UTP30 / Essential nuclear protein 1 / Small ribosomal subunit protein uS11B / U3 small nucleolar RNA-associated protein 7 / U3 small nucleolar ribonucleoprotein protein LCP5 / U3 small nucleolar RNA-associated protein 18 / Regulator of rDNA transcription protein 14 / Protein FAF1 / U3 small nucleolar RNA-associated protein MPP10 / Ribosome biogenesis protein ENP2 / U3 small nucleolar RNA-associated protein 22 / U3 small nucleolar RNA-associated protein 8 / RNA cytidine acetyltransferase / U3 small nucleolar ribonucleoprotein protein IMP4 / U3 small nucleolar RNA-associated protein 5 / U3 small nucleolar RNA-associated protein 15 / rRNA biogenesis protein RRP5 / rRNA-processing protein FCF1 / U3 small nucleolar RNA-associated protein 13 / U3 small nucleolar RNA-associated protein 21 / Ribosomal RNA small subunit methyltransferase NEP1 / Nucleolar complex protein 4 / Protein BFR2 / RNA 3'-terminal phosphate cyclase-like protein / U3 small nucleolar RNA-associated protein 16 / rRNA-processing protein FCF2 / Small ribosomal subunit protein eS28A / Pre-rRNA-processing protein PNO1

• Biological species: Saccharomyces cerevisiae (brewer's yeast)
• Method: single particle reconstruction / cryo EM / Resolution: 3.99 Å
• Authors: Rai J / Zhao Y / Li H

Funding support

United States, 1 items

Organization	Grant number	Country
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)	R01 GM124622	United States

• Citation: Journal: Commun Biol / Year: 2022; Title: Artificial intelligence-assisted cryoEM structure of Bfr2-Lcp5 complex observed in the yeast small subunit processome.; Authors: Yu Zhao / Jay Rai / Chong Xu / Huan He / Hong Li / ; Abstract: Eukaryotic ribosome is maturated through an elaborate process that includes modification, processing and folding of pre-ribosomal RNA (pre-rRNAs) by a series of ribosome assembly intermediates. More than 70 factors participate in the dynamic assembly and disassembly of the small subunit processome (90S) inside nucleolus, leading to the early maturation of small subunit. The 5' domain of the 18S rRNA is the last to be incorporated into the stable 90S prior to the cleavage of pre-rRNA at the A1 site. This step is facilitated by the Kre33-Enp2-Bfr2-Lcp5 protein module with the participation of the DEAD-box protein Dbp4. Though structures of Kre33 and Enp2 have been modeled in previously observed 90S structures, that of Bfr2-Lcp5 complex remains unavailable. Here, we report an AlphaFold-assisted structure determination of the Bfr2-Lcp5 complex captured in a 3.99 Å - 7.24 Å cryoEM structure of 90S isolated from yeast cells depleted of Pih1, a chaperone protein of the 90S core assembly. The structure model is consistent with the protein-protein interaction results and the secondary structures of recombinant Bfr2 and Bfr2-Lcp5 complex obtained by Circular Dichroism. The Bfr2-Lcp5 complex interaction mimics that of exosome factors Rrp6-Rrp47 and acts to regulate 90S transitions.

History

Deposition	Nov 17, 2021	-
Header (metadata) release	Jul 6, 2022	-
Map release	Jul 6, 2022	-
Update	Aug 16, 2023	-
Current status	Aug 16, 2023	Processing site: RCSB / Status: Released

Map

• File: Download / File: emd_25441.map.gz / Format: CCP4 / Size: 307.5 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Annotation: Composite map
• Voxel size: X=Y=Z: 1.074 Å

Density

Contour Level	By AUTHOR: 0.007
Minimum - Maximum	-0.009132517 - 0.0651138
Average (Standard dev.)	0.0012406418 (±0.0028897533)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 432 432 432 Spacing 432 432 432
Cell	A=B=C: 463.968 Å α=β=γ: 90.0 °

Supplemental data

Additional map: Core region (3.99 Angstorm) from 199,534 particles.

• File: emd_25441_additional_1.map
• Annotation: Core region (3.99 Angstorm) from 199,534 particles.

Additional map: Central Domain (7.24 Angstorm) from 199,534 particles.

• File: emd_25441_additional_2.map
• Annotation: Central Domain (7.24 Angstorm) from 199,534 particles.

Additional map: Head domain (5.04 Angstorm) from 84,570 particles.

• File: emd_25441_additional_3.map
• Annotation: Head domain (5.04 Angstorm) from 84,570 particles.

Sample components

Entire : Structure of Bfr2-Lcp5 Complex Observed in the Small Subunit Proc...

• Entire: Name: Structure of Bfr2-Lcp5 Complex Observed in the Small Subunit Processome Isolated from R2TP-depleted Yeast Cells

Components

• Complex: Structure of Bfr2-Lcp5 Complex Observed in the Small Subunit Processome Isolated from R2TP-depleted Yeast Cells
  • Protein or peptide: U3 small nucleolar RNA-associated protein MPP10
  • Protein or peptide: Nucleolar protein 56
  • Protein or peptide: Something about silencing protein 10
  • RNA: 5' ETS
  • RNA: U3 snoRNA
  • Protein or peptide: 40S ribosomal protein S18-A
  • Protein or peptide: 40S ribosomal protein S4-A
  • Protein or peptide: 40S ribosomal protein S5
  • Protein or peptide: 40S ribosomal protein S7-A
  • Protein or peptide: 40S ribosomal protein S8-A
  • Protein or peptide: 40S ribosomal protein S9-A
  • Protein or peptide: 40S ribosomal protein S16-A
  • Protein or peptide: 40S ribosomal protein S11-A
  • Protein or peptide: 40S ribosomal protein S22-A
  • Protein or peptide: 40S ribosomal protein S24-A
  • Protein or peptide: 40S ribosomal protein S28-A
  • Protein or peptide: NET1-associated nuclear protein 1
  • Protein or peptide: U3 small nucleolar RNA-associated protein 15
  • Protein or peptide: U3 small nucleolar RNA-associated protein 9
  • Protein or peptide: U3 small nucleolar RNA-associated protein 5
  • Protein or peptide: U3 small nucleolar RNA-associated protein 10
  • Protein or peptide: U3 small nucleolar RNA-associated protein 4
  • Protein or peptide: Periodic tryptophan protein 2
  • Protein or peptide: U3 small nucleolar RNA-associated protein 6
  • Protein or peptide: U3 small nucleolar RNA-associated protein 12
  • Protein or peptide: U3 small nucleolar RNA-associated protein 18
  • Protein or peptide: U3 small nucleolar RNA-associated protein 21
  • Protein or peptide: Protein SOF1
  • Protein or peptide: Ribosome biogenesis protein ENP2
  • Protein or peptide: U3 small nucleolar RNA-associated protein 7
  • Protein or peptide: U3 small nucleolar ribonucleoprotein protein IMP3
  • Protein or peptide: 40S ribosomal protein S14-B
  • Protein or peptide: KRR1 small subunit processome component
  • Protein or peptide: rRNA 2'-O-methyltransferase fibrillarin
  • Protein or peptide: Ribonucloprotein
  • Protein or peptide: RRP9 isoform 1
  • Protein or peptide: RNA 3'-terminal phosphate cyclase-like protein
  • Protein or peptide: Ribosome biogenesis protein BMS1
  • Protein or peptide: Ribosomal RNA small subunit methyltransferase NEP1
  • Protein or peptide: rRNA-processing protein FCF1
  • Protein or peptide: U3 small nucleolar ribonucleoprotein protein IMP4
  • Protein or peptide: Ribosome biogenesis protein UTP30
  • Protein or peptide: Pre-rRNA-processing protein PNO1
  • Protein or peptide: rRNA-processing protein FCF2
  • Protein or peptide: 40S ribosomal protein S23-A
  • Protein or peptide: U3 small nucleolar RNA-associated protein 14
  • Protein or peptide: Nucleolar complex protein 14
  • Protein or peptide: U3 small nucleolar RNA-associated protein 11
  • Protein or peptide: Essential nuclear protein 1
  • Protein or peptide: rRNA biogenesis protein RRP5
  • Protein or peptide: U3 small nucleolar RNA-associated protein 22
  • Protein or peptide: Ribosomal RNA-processing protein 7
  • RNA: 18S pre-rRNA
  • Protein or peptide: Nucleolar complex protein 4
  • Protein or peptide: U3 small nucleolar RNA-associated protein 8
  • Protein or peptide: Bud site selection protein 21
  • Protein or peptide: U3 small nucleolar RNA-associated protein 13
  • Protein or peptide: Protein FAF1
  • Protein or peptide: Nucleolar protein 58
  • Protein or peptide: Regulator of rDNA transcription protein 14
  • Protein or peptide: U3 small nucleolar RNA-associated protein 20
  • Protein or peptide: RNA cytidine acetyltransferase
  • Protein or peptide: 40S ribosomal protein S6-A
  • Protein or peptide: 40S ribosomal protein S13
  • Protein or peptide: Protein BFR2
  • Protein or peptide: U3 small nucleolar ribonucleoprotein protein LCP5

Supramolecule #1: Structure of Bfr2-Lcp5 Complex Observed in the Small Subunit Proc...

• Supramolecule: Name: Structure of Bfr2-Lcp5 Complex Observed in the Small Subunit Processome Isolated from R2TP-depleted Yeast Cells; type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#66
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)

Macromolecule #1: U3 small nucleolar RNA-associated protein MPP10

• Macromolecule: Name: U3 small nucleolar RNA-associated protein MPP10 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 28.232779 KDa

Sequence

String:

KNLSSFEKQQ IEIRKQIEQL ENEAVAEKKW SLKGEVKAKD RPEDALLTEE LEFDRTAKPV PVITSEVTES LEDMIRRRIQ DSNFDDLQR RTLLDITRKS QRPQFELSDV KSSKSLAEIY EDDYTRAEDE SALSEELQKA HSEISELYAN LVYKLDVLSS V HFVPKPAS TSLEIRVETP TISMEDAQPL YMSNASSLAP QEIYNVGKAE KDGEIRLKNG VAMSKEELTR EDKNRLRRAL KR KRSKA

UniProtKB: U3 small nucleolar RNA-associated protein MPP10

Macromolecule #2: Nucleolar protein 56

• Macromolecule: Name: Nucleolar protein 56 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 45.461172 KDa

Sequence

String:

PIEYLLFEEP TGYAVFKVKL QQDDIGSRLK EVQEQINDFG AFTKLIELVS FAPFKGAAEA LENANDISEG LVSESLKAIL DLNLPKASS KKKNITLAIS DKNLGPSIKE EFPYVDCISN ELAQDLIRGV RLHGEKLFKG LQSGDLERAQ LGLGHAYSRA K VKFSVQKN DNHIIQAIAL LDQLDKDINT FAMRVKEWYG WHFPELAKLV PDNYTFAKLV LFIKDKASLN (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)A LL(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)GQDISETDM ENVCVFAQRV ASLADYRRQL YDYLCE KMH TVAPNLSELI GEVIGARLIS HAGSLTNLSK QAASTVQILG AEKALFRALK TKGNTPKYGL IYHSGFISKA SAKNKGR IS RYLANKCSMA SRIDNYSEEP SNVFGSVLKK QVEQRLEFY

Macromolecule #3: Something about silencing protein 10

• Macromolecule: Name: Something about silencing protein 10 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 20.009535 KDa

Sequence

String:

TDRFKGDDDI P(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)DEAR K RGMHDNNGAD LDDKDYGSED EAVSRSINTQ GENDYYQQVQ RGKQDKKISR KEAHKNAVIA AREGKLAELA ENVSGDGKR AINYQILKNK GLTPKRNKDN RNSRVKKRKK YQKAQKKLKS VRAVYSGGQS GVYEGEKTGI KKGLTRSVKF KN

Macromolecule #6: 40S ribosomal protein S18-A

• Macromolecule: Name: 40S ribosomal protein S18-A / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 14.516669 KDa

Sequence

String:

SLVVQEQGSF QHILRLLNTN VDGNIKIVYA LTTIKGVGRR YSNLVCKKAD VDLHKRAGEL TQEELERIVQ IMQNPTHYKI PAWFLNRQN DITDGKDYHT LANNVESKLR DDLERLKKIR (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

Macromolecule #7: 40S ribosomal protein S4-A

• Macromolecule: Name: 40S ribosomal protein S4-A / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 25.662736 KDa

Sequence

String:

APHHWLLDKL SGCYAPRPSA GPHKLRESLP LIVFLRNRLK YALNGREVKA ILMQRHVKVD GKVRTDTTYP AGFMDVITLD ATNENFRLV YDVKGRFAVH RITDEEASYK LGKVKKVQLG KKGVPYVVTH DGRTIRYPDP NIKVNDTVKI DLASGKITDF I KFDAGKLV YVTGGRNLGR IGTIVHKERH DGGFDLVHIK DSLDNTFVTR LNNVFVIGEQ GKPYISLPKG

UniProtKB: Small ribosomal subunit protein eS4A

Macromolecule #8: 40S ribosomal protein S5

• Macromolecule: Name: 40S ribosomal protein S5 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 23.756203 KDa

Sequence

String:

DFEVVEEFTP VVLATPIPEE VQQAQTEIKL FNKWSFEEVE VKDASLVDYV QVRQPIFVAH TAGRYANKRF RKAQCPIIER LTNSLMMNG RNNGKKLKAV RIIKHTLDII NVLTDQNPIQ VVVDAITNTG PREDTTRVGG GGAARRQAVD VSPLRRVNQA I ALLTIGAR EAAFRNIKTI AETLAEELIN AAKGSSTSYA IKKKDELERV AKSNR

UniProtKB: Small ribosomal subunit protein uS7

Macromolecule #9: 40S ribosomal protein S7-A

• Macromolecule: Name: 40S ribosomal protein S7-A / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 21.658209 KDa

Sequence

String:

MSAPQAKILS QAPTELELQV AQAFVELENS SPELKAELRP LQFKSIREID VAGGKKALAI FVPVPSLAGF HKVQTKLTRE LEKKFQDRH VIFLAERRIL PKPSRTSRQV QKRPRSRTLT AVHDKILEDL VFPTEIVGKR VRYLVGGNKI QKVLLDSKDV Q QIDYKLES FQAVYNKLTG KQIVFEIPSE TH

UniProtKB: Small ribosomal subunit protein eS7A

Macromolecule #10: 40S ribosomal protein S8-A

• Macromolecule: Name: 40S ribosomal protein S8-A / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 22.537803 KDa

Sequence

String:

MGISRDSRHK RSATGAKRAQ FRKKRKFELG RQPANTKIGA KRIHSVRTRG GNKKYRALRI ETGNFSWASE GISKKTRIAG VVYHPSNNE LVRTNTLTKA AIVQIDATPF RQWFEAHYGQ TLGKKKNVKE EETVAKSKNA ERKWAARAAS AKIESSVESQ F SAGRLYAC ISSRPGQSGR CDGYILEGEE LAFYLRRLTA KK

UniProtKB: Small ribosomal subunit protein eS8A

Macromolecule #11: 40S ribosomal protein S9-A

• Macromolecule: Name: 40S ribosomal protein S9-A / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 20.093445 KDa

Sequence

String:

KTYSTPKRPY ESSRLDAELK LAGEFGLKNK KEIYRISFQL SKIRRAARDL LTRDEKDPKR LFEGNALIRR LVRVGVLSED KKKLDYVLA LKVEDFLERR LQTQVYKLGL AKSVHHARVL ITQRHIAVGK QIVNIPSFMV RLDSEKHIDF APTSPFGGAR P GRVARRNA ARKAEAS

UniProtKB: Small ribosomal subunit protein uS4A

Macromolecule #12: 40S ribosomal protein S16-A

• Macromolecule: Name: 40S ribosomal protein S16-A / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 13.798053 KDa

Sequence

String:

AVPSVQTFGK KKSATAVAHV KAGKGLIKVN GSPITLVEPE ILRFKVYEPL LLVGLDKFSN IDIRVRVTGG GHVSQVYAIR QAIAKGLVA YHQKYVDEQS KNELKKAFTS YDRTLLIADS RRPEPK

UniProtKB: Small ribosomal subunit protein uS9A

Macromolecule #13: 40S ribosomal protein S11-A

• Macromolecule: Name: 40S ribosomal protein S11-A / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 17.785934 KDa

Sequence

String:

MSTELTVQSE RAFQKQPHIF NNPKVKTSKR TKRWYKNAGL GFKTPKTAIE GSYIDKKCPF TGLVSIRGKI LTGTVVSTKM HRTIVIRRA YLHYIPKYNR YEKRHKNVPV HVSPAFRVQV GDIVTVGQCR PISKTVRFNV VKVSAAAGKA NKQFAKF

UniProtKB: Small ribosomal subunit protein uS17A

Macromolecule #14: 40S ribosomal protein S22-A

• Macromolecule: Name: 40S ribosomal protein S22-A / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 14.260569 KDa

Sequence

String:

SSVLADALNA INNAEKTGKR QVLIRPSSKV IIKFLQVMQK HGYIGEFEYI DDHRSGKIVV QLNGRLNKCG VISPRFNVKI GDIEKWTAN LLPARQFGYV ILTTSAGIMD HEEARRKHVS GKILGFVY

UniProtKB: Small ribosomal subunit protein uS8A

Macromolecule #15: 40S ribosomal protein S24-A

• Macromolecule: Name: 40S ribosomal protein S24-A / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 10.130715 KDa

Sequence

String:

AVTIRTRKVI SNPLLARKQF VVDVLHPNRA NVSKDELREK LAEVYKAEKD AVSVFGFRTQ FGGGKSVGFG LVYNSVAEAK KFEPTYRLV R

UniProtKB: Small ribosomal subunit protein eS24A

Macromolecule #16: 40S ribosomal protein S28-A

• Macromolecule: Name: 40S ribosomal protein S28-A / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 7.116281 KDa

Sequence

String:

TPVTLAKVIK VLGRTGSRGG VTQVRVEFLE DTSRTIVRNV KGPVRENDIL VLMESEREAR RLR

UniProtKB: Small ribosomal subunit protein eS28A

Macromolecule #17: NET1-associated nuclear protein 1

• Macromolecule: Name: NET1-associated nuclear protein 1 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 100.91143 KDa

Sequence

String:

MTQSLGIEQY KLSVVSGGKP ALNNLSSVTG NKNIARLSQD QRNYIIPFNN QIKVYSVETR QCVKTLKFAN NSLLSGIFLQ EEENNESIV KILLGDITVP QQEDAHLITV FTNNGHVIVL NYKGKLVESP KHFKISLADE KLANVFHSEG NYRILTTFKD P SQKAHNSL QSYRLYALTF DDAKKQFEVA HQAEWHNVIL SNISSNGKLL AHMCKDVSTK DHEHKSISVV SLFDDSVNLS FP LGSILSS QTQSLSYNTR YVSSMAIDNM GQQLAVGFAS GVISIVSLAD LQIRLLKWHI DSVLSLSFSH DGSYLLSGGW EKV MSLWQL ETNSQQFLPR LNGIIIDCQV LGPQGNYYSL ILQMTENNSN SDYQFLLLNA SDLTSKLSIN GPLPVFNSTI KHIQ QPISA MNTKNSNSIT SLNHSKKKQS RKLIKSRRQD FTTNVEINPI NKNLYFPHIS AVQIFDFYKN EQVNYQYLTS GVNNS MGKV RFELNLQDPI ITDLKFTKDG QWMITYEIEY PPNDLLSSKD LTHILKFWTK NDNETNWNLK TKVINPHGIS VPITKI LPS PRSVNNSQGC LTADNNGGLK FWSFDSHESN WCLKKISLPN FNHFSNSVSL AWSQDGSLIF HGFDDKLQIL DFDTFKK FE SLENTKTVSE FTLDSEIQTV KLINDTNLIV ATRTTLNAIN LLRGQVINSF DLYPFVNGVY KNGHMDRLIT CDERTGNI A LVINQQLTDL DGVPTINYKS RIIIFDSDLS TKLGNFTHHE YISWIGWNYD TDFIFLDIES TLGVVGTTVN TQLSDEVNN EGILDGLVSN TITTSASNSD IFAEQLHKLS S(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)NDED EEDIALEFIN GEKKDKLVNM NSFTSMFDNI QNVQMDTFFD RVMKVLT

Macromolecule #18: U3 small nucleolar RNA-associated protein 15

• Macromolecule: Name: U3 small nucleolar RNA-associated protein 15 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 57.765289 KDa

Sequence

String:

MSTARPRIIT SKAPLLPQQT TPEQRYWRQY TSAQLVKEHN SVTHISFNPQ HPHDFAVTSS TRVQIFSSRT RQVIKTFSRF KDVVYSASF RSDGKLLCAG DATGLVSVYD SYNPRTILLS INASTHPTHV TKFHTQDNKI LATASDDRVT RLWDISNAYE P QLELTGAT DYVRTLSFIP AAPHLVATGS YDGLIRLYDT RSSGSTPIYS LNHDQPVENV IAVSPTQIVS CGGNNFKVWD LT SNKKLYE RGNFNKAVTC LDYVENFDSP MQSALIASSL DGHVKVFDPL DNFQVKFGWK FSGPVLSCAV SPSTAQGNRH LVA GLSSGL LAIRTKKKEK RSSDKENAPA SFNKNAKSNN FQRMMRGSEY QGDQEHIIHN DKVRSQRRMR AFERNINQFK WSEA LDNAF VPGMAKELTL TVLQELRKRG KVRVALYGRD ESTLEPLLNW CLKGIEDVRS ASIVADWVAV VLELYGNTLE SSPVL QELM IDLKTKVRHE IHKSKEAQRI EGMLQLLTS

UniProtKB: U3 small nucleolar RNA-associated protein 15

Macromolecule #19: U3 small nucleolar RNA-associated protein 9

• Macromolecule: Name: U3 small nucleolar RNA-associated protein 9 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 13.282782 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)DHL SKIFLTISKS ITQNPWNEEN LLPLWLKWLL TLKSGELNSI KDKHTKKNCK HLKSALRSSE EI LPVLLGI QGRLEMLRRQ AKLRED

Macromolecule #20: U3 small nucleolar RNA-associated protein 5

• Macromolecule: Name: U3 small nucleolar RNA-associated protein 5 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 62.177625 KDa

Sequence

String:

SPVLQSAYDP SGQYLCYVTV ALDKQRVGVQ PTQRATSSGV DTVWNENFLY LEDSKLKVTC LKWVNLASSD TVAIILGMNN GEIWLYSVL ANEVTYKFTT GNSYEIKDID LMGNQLWCID SSDAFYQFDL LQFKLLQHFR INNCVQLNKL TIVPAGDSVA Q LLVASHSI SLIDIEEKKV VMTFPGHVSP VSTLQVITNE FFISGAEGDR FLNVYDIHSG MTKCVLVAES DIKELSHSGQ AD SIAVTTE DGSLEIFVDP LVSSSTKKRG NKSKKSSKKI QIVSKDGRKV PIYNAFINKD LLNVSWLQNA TMPYFKNLQW REI PNEYTV EISLNWNNKN KSADRDLHGK DLASATNYVE GNARVTSGDN FKHVDDAIKS WERELTSLEQ EQAKPPQANE LLTE TFGDK LESSTVARIS GKKTNLKGSN LKTATTTGTV TVILSQALQS NDHSLLETVL NNRDERVIRD TIFRLKPALA VILLE RLAE RIARQTHRQG PLNVWVKWCL IIHGGYLVSI PNLMSTLSSL HSTLKRRSDL LPRLLALDAR LDCTINKFKT L

UniProtKB: U3 small nucleolar RNA-associated protein 5

Macromolecule #21: U3 small nucleolar RNA-associated protein 10

• Macromolecule: Name: U3 small nucleolar RNA-associated protein 10 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 49.044969 KDa

Sequence

String:

SSLSDQLAQV ASNNATVALD RKRRQKLHSA SLIYNSKTAA TQDYDFIFEN ASKALEELSQ IEPKFAIFSR TLFSESSISL DRNVQTKEE IKDLDNAINA YLLLASSKWY LAPTLHATEW LVRRFQIHVK NTEMLLLSTL NYYQTPVFKR ILSIIKLPPL F NCLSNFVR SEKPPTALTM IKLFNDMDFL KLYTSYLDQC IKHNATYTNQ LLFTTCCFIN VVAFNSNNDE KLNQLVPILL EI SAKLLAS KSKDCQIAAH TILVVFATAL PLKKTIILAA METILSNLDA KEAKHSALLT ICKLFQTLKG QGNVDQLPSK IFK LFDSKF DTVSILTFLD KEDKPVCDKF ITSYTRSIAR YDRSKLNIIL SLLKKIRLER YEVRLIITDL IYLSEILEDK SQLV ELFEY FISINEDLVL KCLKSLGLTG (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

Macromolecule #22: U3 small nucleolar RNA-associated protein 4

• Macromolecule: Name: U3 small nucleolar RNA-associated protein 4 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 84.357133 KDa

Sequence

String:

ERMIVHRCRF VDFTPATITS LAFSHKSNIN KLTPSDLRLA IGRSNGNIEI WNPRNNWFQE MVIEGGKDRS IEGLCWSNVN GESLRLFSI GGSTVVTEWD LATGLPLRNY DCNSGVIWSI SINDSQDKLS VGCDNGTVVL IDISGGPGVL EHDTILMRQE A RVLTLAWK KDDFVIGGCS DGRIRIWSAQ KNDENMGRLL HTMKVDKAKK ESTLVWSVIY LPRTDQIASG DSTGSIKFWD FQ FATLNQS FKAHDADVLC LTTDTDNNYV FSAGVDRKIF QFSQNTNKSQ KNNRWVNSSN RLLHGNDIRA ICAYQSKGAD FLV SGGVEK TLVINSLTSF SNGNYRKMPT VEPYSKNVLV NKEQRLVVSW SESTVKIWTM GTDSSTEQNY KLVCKLTLKD DQNI STCSL SPDGQVLVVG RPSTTKVFHL QPVGNKLKVT KLDNDLLLRT STKLVKFIDN SKIVICSCED DVFIVDLESE EDEKP QEVE LLEVTSTKSS IKVPYINRIN HLEVDQNIAV ISRGCGVVDI LDLKARISKP LARLNNFITA VHINTSRKSV VVITAD NKI YEFNMNLNSE AENEDSESVL TQWSKNNTDN LPKEWKTLKE NCVGIFSDIE NSSRLWFWGA TWISRIDFDV DFPINKR RK QKKRTHEGLT ITDESNFMND EEDDEDDDID MEISENLNVL LNQGN(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)HFFFTDKYK PLLFVDLISS NELAIIERNP LTFHSK QKA FIQPKLVF

Macromolecule #23: Periodic tryptophan protein 2

• Macromolecule: Name: Periodic tryptophan protein 2 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 96.434289 KDa

Sequence

String:

KSDFKFSNLL GTVYRQGNIT FSDDGKQLLS PVGNRVSVFD LINNKSFTFE YEHRKNIAAI DLNKQGTLLI SIDEDGRAIL VNFKARNVL HHFNFKEKCS AVKFSPDGRL FALASGRFLQ IWKTPDVNKD RQFAPFVRHR VHAGHFQDIT SLTWSQDSRF I LTTSKDLS AKIWSVDSEE KNLAATTFNG HRDYVMGAFF SHDQEKIYTV SKDGAVFVWE FTKRPSDDDD NESEDDDKQE EV DISKYSW RITKKHFFYA NQAKVKCVTF HPATRLLAVG FTSGEFRLYD LPDFTLIQQL SMGQNPVNTV SVNQTGEWLA FGS SKLGQL LVYEWQSESY ILKQQGHFDS TNSLAYSPDG SRVVTASEDG KIKVWDITSG FCLATFEEHT SSVTAVQFAK RGQV MFSSS LDGTVRAWDL IRYRNFRTFT GTERIQFNCL AVDPSGEVVC AGSLDNFDIH VWSVQTGQLL DALSGHEGPV SCLSF SQEN SVLASASWDK TIRIWSIFGR SQQVEPIEVY SDVLALSMRP DGKEVAVSTL KGQISIFNIE DAKQVGNIDC RKDIIS GRF NQDRFTAKNS ERSKFFTTIH YSFDGMAIVA GGNNNSICLY DVPNEVLLKR FIVSRNMALN GTLEFLNSKK MTEAGSL DL IDDAGENSDL EDRIDNSLPG SQRGGDLSTR KMRPEVRVTS VQFSPTANAF AAASTEGLLI YSTNDTILFD PFDLDVDV T PHSTVEALRE KQFLNALVMA FRLNEEYLIN KVYEAIPIKE IPLVASNIPA IYLPRILKFI GDFAIESQHI EFNLIWIKA LLSASGGYIN EHKYLFSTAM RSIQRFIVRV AKEVVNTTTD NKYTYRFLVS TDGSM

UniProtKB: Periodic tryptophan protein 2

Macromolecule #24: U3 small nucleolar RNA-associated protein 6

• Macromolecule: Name: U3 small nucleolar RNA-associated protein 6 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 46.689199 KDa

Sequence

String:

SKTRYYLEQC IPEMDDLVEK GLFTKNEVSL IMKKRTDFEH RLNSRGSSIN DYIKYINYES NVNKLRAKRC KRILQVKKTN SLSDWSIQQ RIGFIYQRGT NKFPQDLKFW AMYLNYMKAR GNQTSYKKIH NIYNQLLKLH PTNVDIWISC AKYEYEVHAN F KSCRNIFQ NGLRFNPDVP KLWYEYVKFE LNFITKLINR RKVMGLINER EQELDMQNEQ KNNQAPDEEK SHLQVPSTGD SM KDKLNEL PEADISVLGN AETNPALRGD IALTIFDVCM KTLGKHYINK HKGYYAISDS KMNIELNKET LNYLFSESLR YIK LFDEF(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

Macromolecule #25: U3 small nucleolar RNA-associated protein 12

• Macromolecule: Name: U3 small nucleolar RNA-associated protein 12 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 104.697141 KDa

Sequence

String:

YQRFEQAAAF GVIASNANCV WIPASSGNSN GSGPGQLITS ALEDVNIWDI KTGDLVSKLS DGLPPGASDA RGAKPAECTY LEAHKDTDL LAVGYADGVI KVWDLMSKTV LLNFNGHKAA ITLLQFDGTG TRLISGSKDS NIIVWDLVGE VGLYKLRSHK D SITGFWCQ GEDWLISTSK DGMIKLWDLK THQCIETHIA HTGECWGLAV KDDLLITTGT DSQVKIWKLD IENDKMGGKL TE MGIFEKQ SKQRGLKIEF ITNSSDKTSF FYIQNADKTI ETFRIR(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)KG(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)KE SYSSFILHPF QTIRSLYKIK SASWTTVSSS KLELVLTTSS NTIEYYSI P YEKRDPTSPA PLKTHTIELQ GQRTDVRSID ISDDNKLLAT ASNGSLKIWN IKTHKCIRTF ECGYALTCKF LPGGLLVIL GTRNGELQLF DLASSSLLDT IEDAHDAAIW SLDLTSDGKR LVTGSADKTV KFWDFKVENS LVPGTKNKFL PVLKLHHDTT LELTDDILC VRVSPDDRYL AISLLDNTVK VFFLDSMKFY LSLYGHKLPV LSIDISFDSK MIITSSADKN IKIWGLDFGD C HKSLFAHQ DSIMNVKFLP QSHNFFSCSK DAVVKYWDGE KFECIQKLYA HQSEVWALAV ATDGGFVVSS SHDHSIRIWE ET EDQVFLE EEKEKELEEQ YEDTLLTSLE EGNGDDAFKA DASGEGVEDE ASGVHKQTLE SLKAGERLME ALDLGIAEIE GLE AYNRDM KL(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)KPQGNAV LIAVNKTPEQ YIMDTLLRI RMSQLEDALM VMPFSYVLKF LKFIDTVMQN KTLLHSHLPL ICKNLFFIIK FNHKELVSQK NEELKLQINR V KTELRSAL KSTEDDLGFN VQGLKFVKQQ WNLRHNYEFV DEYDQQEKES NSARKRVFGT VI

Macromolecule #26: U3 small nucleolar RNA-associated protein 18

• Macromolecule: Name: U3 small nucleolar RNA-associated protein 18 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 66.49425 KDa

Sequence

String:

MTMATTAMNV SVPPPDEEEQ LLAKFVFGDT TDLQENLAKF NADFIFNEQE MDVEDQEDEG SESDNSEEDE AQNGELDHVN NDQLFFVDD GGNEDSQDKN EDTMDVDDED DSSSDDYSED SEEAAWIDSD DEKIKVPILV TNKTKKLRTS YNESKINGVH Y INRLRSQF EKIYPRPKWV DDESDSELDD EEDDEEEGSN NVINGDINAL TKILSTTYNY KDTLSNSKLL PPKKLDIVRL KD ANASHPS HSAIQSLSFH PSKPLLLTGG YDKTLRIYHI DGKTNHLVTS LHLVGSPIQT CTFYTSLSNQ NQQNIFTAGR RRY MHSWDL SLENLTHSQT AKIEKFSRLY GHESTQRSFE NFKVAHLQNS QTNSVHGIVL LQGNNGWINI LHSTSGLWLM GCKI EGVIT DFCIDYQPIS RGKFRTILIA VNAYGEVWEF DLNKNGHVIR RWKDQGGVGI TKIQVGGGTT TTCPALQISK IKQNR WLAV GSESGFVNLY DRNNAMTSST PTPVAALDQL TTTISNLQFS PDGQILCMAS RAVKDALRLV HLPSCSVFSN WPTSGT PLG KVTSVAFSPS GGLLAVGNEQ GKVRLWKLNH Y

UniProtKB: U3 small nucleolar RNA-associated protein 18

Macromolecule #27: U3 small nucleolar RNA-associated protein 21

• Macromolecule: Name: U3 small nucleolar RNA-associated protein 21 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 102.793328 KDa

Sequence

String:

KNSKIFSPFR IIGNVSNGVP FATGTLGSTF YIVTCVGKTF QIYDANTLHL LFVSEKETPS SIVALSAHFH YVYAAYENKV GIYKRGIEE HLLELETDAN VEHLCIFGDY LCASTDDNSI FIYKKSDPQD KYPSEFYTKL TVTEIQGGEI VSLQHLATYL N KLTVVTKS NVLLFNVRTG KLVFTSNEFP DQITTAEPAP VLDIIALGTV TGEVIMFNMR KGKRIRTIKI PQSRISSLSF RT DGSSHLS VGTSSGDLIF YDLDRRSRIH VLKNIHRESY GGVTQATFLN GQPIIVTSGG DNSLKEYVFD PSLSQGSGDV VVQ PPRYLR SRGGHSQPPS YIAFADSQSH FMLSASKDRS LWSFSLRKDA QSQEMSQRLH KKQDGGRVGG STIKSKFPEI VALA IENAR IGEWENIITA HKDEKFARTW DMRNKRVGRW TFDTTDDGFV KSVAMSQCGN FGFIGSSNGS ITIYNMQSGI LRKKY KLHK RAVTGISLDG MNRKMVSCGL DGIVGFYDFN KSTLLGKLKL DAPITAMVYH RSSDLFALAL DDLSIVVIDA VTQRVV RQL WGHSNRITAF DFSPEGRWIV SASLDSTIRT WDLPTGGCID GIIVDNVATN VKFSPNGDLL ATTHVTGNGI CIWTNRA QF KTVSTRTIDE SEFARMALPS TSVRGNDSML SGALESNGGE DLNDIDFNTY TSLEQIDKEL LTLSIGPRSK MNTLLHLD V IRKRSKPKEA PKKSEKLPFF LQLSGEKVGD EASVREGIAH ETPEEIHRRD QEAQKKLDAE EQMNKFKVTG RLGFESHFT KQLREGSQSK DYSSLLATLI NFSPAAVDLE IRSLNSFEPF DEIVWFIDAL TQGLKSNKNF ELYETFMSLL FKAHGDVIHA NNKNQDIAS ALQNWEDVHK KEDRLDDLVK FCMGVAAFVT TA

UniProtKB: U3 small nucleolar RNA-associated protein 21

Macromolecule #28: Protein SOF1

• Macromolecule: Name: Protein SOF1 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 53.819594 KDa

Sequence

String:

MKIKTIKRSA DDYVPVKSTQ ESQMPRNLNP ELHPFERARE YTKALNATKL ERMFAKPFVG QLGYGHRDGV YAIAKNYGSL NKLATGSAD GVIKYWNMST REEFVSFKAH YGLVTGLCVT QPRFHDKKPD LKSQNFMLSC SDDKTVKLWS INVDDYSNKN S SDNDSVTN EEGLIRTFDG ESAFQGIDSH RENSTFATGG AKIHLWDVNR LKPVSDLSWG ADNITSLKFN QNETDILAST GS DNSIVLY DLRTNSPTQK IVQTMRTNAI CWNPMEAFNF VTANEDHNAY YYDMRNLSRS LNVFKDHVSA VMDVDFSPTG DEI VTGSYD KSIRIYKTNH GHSREIYHTK RMQHVFQVKY SMDSKYIISG SDDGNVRLWR SKAWERSNVK TTREKNKLEY DEKL KERFR HMPEIKRISR HRHVPQVIKK AQEIKNIELS SIKRREANER RTRKDMPYIS ERKKQIVGTV

UniProtKB: Protein SOF1

Macromolecule #29: Ribosome biogenesis protein ENP2

• Macromolecule: Name: Ribosome biogenesis protein ENP2 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 41.373207 KDa

Sequence

String:

VLKSTSANDV SVYQVSGTNV SRSLPDWIAK KRKRQLKNDL EYQNRVELIQ DFEFSEASNK IKVSRDGQYC MATGTYKPQI HVYDFANLS LKFDRHTDAE NVDFTILSDD WTKSVHLQND RSIQFQNKGG LHYTTRIPKF GRSLVYNKVN CDLYVGASGN E LYRLNLEK GRFLNPFKLD TEGVNHVSIN EVNGLLAAGT ETNVVEFWDP RSRSRVSKLY LENNIDNRPF QVTTTSFRND GL TFACGTS NGYSYIYDLR TSEPSIIKDQ GYGFDIKKII WLDNVGTENK IVTCDKRIAK IWDRLDGKAY ASMEPSVDIN DIE HVPGTG MFFTANESIP MHTYYIPSLG PSPRWCSFLD SITEEL

UniProtKB: Ribosome biogenesis protein ENP2

Macromolecule #30: U3 small nucleolar RNA-associated protein 7

• Macromolecule: Name: U3 small nucleolar RNA-associated protein 7 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 48.73109 KDa

Sequence

String:

QTKDKKLRAG LKKIDEQYKK AVSSAAATDY LLPESNGYLE PENELEKTFK VQQSEIKSSV DVSTANKALD LSLKEFGPYH IKYAKNGTH LLITGRKGHV ASMDWRKGQL RAELFLNETC HSATYLQNEQ YFAVAQKKYT FIYDHEGTEL HRLKQHIEAR H LDFLPYHY LLVTAGETGW LKYHDVSTGQ LVSELRTKAG PTMAMAQNPW NAVMHLGHSN GTVSLWSPSM PEPLVKLLSA RG PVNSIAI DRSGYYMATT GADRSMKIWD IRNFKQLHSV ESLPTPGTNV SISDTGLLAL SRGPHVTLWK DALKLSGDSK PCF GSMGGN PHRNTPYMSH LFAGNKVENL GFVPFEDLLG VGHQTGITNL IVPGAGEANY DALELNPFET KKQRQEQEVR TLLN KLPAD TITLDPNSIG SVDKRSSTIR LNAKDLAQTT MDA

UniProtKB: U3 small nucleolar RNA-associated protein 7

Macromolecule #31: U3 small nucleolar ribonucleoprotein protein IMP3

• Macromolecule: Name: U3 small nucleolar ribonucleoprotein protein IMP3 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 21.797334 KDa

Sequence

String:

VRKLKHHEQK LLKKVDFLEW KQDQGHRDTQ VMRTYHIQNR EDYHKYNRIC GDIRRLANKL SLLPPTDPFR RKHEQLLLDK LYAMGVLTT KSKISDLENK VTVSAICRRR LPVIMHRLKM AETIQDAVKF IEQGHVRVGP NLINDPAYLV TRNMEDYVTW V DNSKIKKT LLRYRNQIDD FDFS

UniProtKB: U3 small nucleolar ribonucleoprotein protein IMP3

Macromolecule #32: 40S ribosomal protein S14-B

• Macromolecule: Name: 40S ribosomal protein S14-B / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 11.645293 KDa

Sequence

String:

NSQVFGVARI YASFNDTFVH VTDLSGKETI ARVTGGMKVK ADRDESSPYA AMLAAQDVAA KCKEVGITAV HVKIRATGGT RTKTPGPGG QAALRALARS GLRIGRIEDV TP

UniProtKB: Small ribosomal subunit protein uS11B

Macromolecule #33: KRR1 small subunit processome component

• Macromolecule: Name: KRR1 small subunit processome component / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 20.17099 KDa

Sequence

String:

ESSFMTLFPK YRESYLKTIW NDVTRALDKH NIACVLDLVE GSMTVKTTRK TYDPAIILKA RDLIKLLARS VPFPQAVKIL QDDMACDVI KIGNFVTNKE RFVKRRQRLV GPNGNTLKAL ELLTKCYILV QGNTVSAMGP FKGLKEVRRV VEDCMKNIHP I YHIKELMI KRELAKR

UniProtKB: KRR1 small subunit processome component

Macromolecule #34: rRNA 2'-O-methyltransferase fibrillarin

• Macromolecule: Name: rRNA 2'-O-methyltransferase fibrillarin / type: protein_or_peptide / ID: 34 / Number of copies: 2 / Enantiomer: LEVO; EC number: Transferases; Transferring one-carbon groups; Methyltransferases
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 27.341668 KDa

Sequence

String:

ARGGAKVVIE PHRHAGVYIA RGKEDLLVTK NMAPGESVYG EKRISVEEPS KEDGVPPTKV EYRVWNPFRS KLAAGIMGGL DELFIAPGK KVLYLGAASG TSVSHVSDVV GPEGVVYAVE FSHRPGRELI SMAKKRPNII PIIEDARHPQ KYRMLIGMVD C VFADVAQP DQARIIALNS HMFLKDQGGV VISIKANCID STVDAETVFA REVQKLREER IKPLEQLTLE PYERDHCIVV GR YMRSGLK

UniProtKB: rRNA 2'-O-methyltransferase fibrillarin

Macromolecule #35: Ribonucloprotein

• Macromolecule: Name: Ribonucloprotein / type: protein_or_peptide / ID: 35 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 13.083232 KDa

Sequence

String:

NPKAFPLADA ALTQQILDVV QQAANLRQLK KGANEATKTL NRGISEFIIM AADCEPIEIL LHLPLLCEDK NVPYVFVPSR VALGRACGV SRPVIAASIT TNDASAIKTQ IYAVKDKIET LL

UniProtKB: Ribonucloprotein

Macromolecule #36: RRP9 isoform 1

• Macromolecule: Name: RRP9 isoform 1 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 52.527512 KDa

Sequence

String:

FDAGDLDKDI IASRLKEDVA EQQGRVFRYF GDKLLISEAK QSFTRVGENN LTCISCFQPV LNKYTFEESS NGDKNKGRLF AYTVSKDLQ LTKYDITDFS KRPKKLKYAK GGAKYIPTSK HEYENTTEGH YDEILTVAAS PDGKYVVTGG RDRKLIVWST E SLSPVKVI PTKDRRGEVL SLAFRKNSDQ LYASCADFKI RTYSINQFSQ LEILYGHHDI VEDISALAME RCVTVGARDR TA MLWKIPD ETRLTFRGGD EPQKLLRRWM KENAKEGEDG EVKYPDESEA PLFFCEGSID VVSMVDDFHF ITGSDNGNIC LWS LAKKKP IFTERIAHGI LPEPSFNDIS GETDEELRKR QLQGKKLLQP FWITSLYAIP YSNVFISGSW SGSLKVWKIS DNLR SFELL GELSGAKGVV TKIQVVESGK HGKEKFRILA SIAKEHRLGR WIANVSGARN GIYSAVIDQ

UniProtKB: RRP9 isoform 1

Macromolecule #37: RNA 3'-terminal phosphate cyclase-like protein

• Macromolecule: Name: RNA 3'-terminal phosphate cyclase-like protein / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 39.588859 KDa

Sequence

String:

KYTTFQGSQN FRLRIVLATL SGKPIKIEKI RSGDLNPGLK DYEVSFLRLI ESVTNGSVIE ISYTGTTVIY RPGIIVGGAS THICPSSKP VGYFVEPMLY LAPFSKKKFS ILFKGITASH NDAGIEAIKW GLMPVMEKFG VRECALHTLK RGSPPLGGGE V HLVVDSLI AQPITMHEID RPIISSITGV AYSTRVSPSL VNRMIDGAKK VLKNLQCEVN ITADVWRGEN SGKSPGWGIT LV AQSKQKG WSYFAEDIGD AGSIPEELGE KVACQLLEEI SKSAAVGRNQ LPLAIVYMVI GKEDIGRLRI NKEQIDERFI ILL RDIKKI FNTEVFLKPV DEADNEDMIA TIKGIGFTNT SKKI

UniProtKB: RNA 3'-terminal phosphate cyclase-like protein

Macromolecule #38: Ribosome biogenesis protein BMS1

• Macromolecule: Name: Ribosome biogenesis protein BMS1 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 127.88968 KDa

Sequence

String:

TMQRSSDVNE RKLHVPMVDR TPEDDPPPFI VAVVGPPGTG KTTLIRSLVR RMTKSTLNDI QGPITVVSGK HRRLTFLECP ADDLNAMID IAKIADLVLL LIDGNFGFEM ETMEFLNIAQ HHGMPRVLGV ATHLDLFKSQ STLRASKKRL KHRFWTEVYQ G AKLFYLSG VINGRYPDRE ILNLSRFISV MKFRPLKWRN EHPYMLADRF TDLTHPELIE TQGLQIDRKV AIYGYLHGTP LP SAPGTRV HIAGVGDFSV AQIEKLPDPC PTPFYQQKLD DFEREKMKEE AKANGEITTA STTRRRKRLD DKDKLIYAPM SDV GGVLMD KDAVYIDIGK KNEEPSFVPG QERGEGEKLM TGLQSVEQSI AEKFDGVGLQ LFSNGTELHE VADHEGMDVE SGEE SIEDD EGKSKGRTSL RKPRIYGKPV QEEDADIDNL PSDEEPYTND DDVQDSEPRM VEIDFNNTGE QGAEKLALET DS (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)NIGKLIY MDNISPEE C IRRWRGEDDD SKDESDIEED VDDDFFRKKD GTVTKEGNKD HAVDLEKFVP YFDTFEKLAK KWKSVDAIKE RFLGAGILG NDNKTKSDSN EGGEELYGDF EDLEDGNPSE QAEDNSDKES EDEDENEDTN GDDDNSFTNF DAEEKKDLTM EQEREMNAAK KEKLRAQFE IEEGENFKED DENNEYDTWY ELQKAKISKQ LEINNIEYQE MTPEQRQRIE GFKAGSYVRI VFEKVPMEFV K NFNPKFPI VMGGLLPTEI KFGIVKARLR RHRWHKKILK TNDPLVLSLG WRRFQTLPIY TTTDSRTRTR MLKYTPEHTY CN AAFYGPL CSPNTPFCGV QIVANSDTGN GFRIAATGIV EEIDVNIEIV KKLKLVGFPY KIFKNTAFIK DMFSSAMEVA RFE GAQIKT VSGIRGEIKR ALSKPEGHYR AAFEDKILMS DIVILRSWYP VRVKKFYNPV TSLLLKEKTE WKGLRLTGQI RAAM NLETP SNPDSAYHKI ERVERHFNGL KVPKAVQKEL PFKSQIHQMK PQKKKTYMAK RAVVLGGDEK KARSFIQKVL TISKA KDSK RKEQKASQRK ERLKKLAKME EEKSQRDKEK KKEYFAQN

Macromolecule #39: Ribosomal RNA small subunit methyltransferase NEP1

• Macromolecule: Name: Ribosomal RNA small subunit methyltransferase NEP1 / type: protein_or_peptide / ID: 39 / Number of copies: 2 / Enantiomer: LEVO; EC number: rRNA small subunit pseudouridine methyltransferase Nep1
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 26.19048 KDa

Sequence

String:

QKALPASLVP QAPPVLTSKD KITKRMIVVL AMASLETHKI SSNGPGGDKY VLLNCDDHQG LLKKMGRDIS EARPDITHQC LLTLLDSPI NKAGKLQVYI QTSRGILIEV NPTVRIPRTF KRFSGLMVQL LHKLSIRSVN SEEKLLKVIK NPITDHLPTK C RKVTLSFD APVIRVQDYI EKLDDDESIC VFVGAMARGK DNFADEYVDE KVGLSNYPLS ASVACSKFCH GAEDAWNI

UniProtKB: Ribosomal RNA small subunit methyltransferase NEP1

Macromolecule #40: rRNA-processing protein FCF1

• Macromolecule: Name: rRNA-processing protein FCF1 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 21.003861 KDa

Sequence

String:

TRKFGLVKRT LNTKKDQRLK KNQENIKTKE DPELTRNIPQ VSSALFFQYN QAIKPPYQVL IDTNFINFSI QKKVDIVRGM MDCLLAKCN PLITDCVMAE LEKLGPKYRI ALKLARDPRI KRLSCSHKGT YADDCLVHRV LQHKCYIVAT NDAGLKQRIR K IPGIPLMS VGGHAYVIEK LPDVF

UniProtKB: rRNA-processing protein FCF1

Macromolecule #41: U3 small nucleolar ribonucleoprotein protein IMP4

• Macromolecule: Name: U3 small nucleolar ribonucleoprotein protein IMP4 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 33.536168 KDa

Sequence

String:

MLRRQARERR EYLYRKAQEL QDSQLQQKRQ IIKQALAQGK PLPKELAEDE SLQKDFRYDQ SLKESEEADD LQVDDEYAAT SGIMDPRII VTTSRDPSTR LSQFAKEIKL LFPNAVRLNR GNYVMPNLVD ACKKSGTTDL VVLHEHRGVP TSLTISHFPH G PTAQFSLH NVVMRHDIIN AGNQSEVNPH LIFDNFTTAL GKRVVCILKH LFNAGPKKDS ERVITFANRG DFISVRQHVY VR TREGVEI AEVGPRFEMR LFELRLGTLE NKDADVEWQL RRFIRTANKK DYL

UniProtKB: U3 small nucleolar ribonucleoprotein protein IMP4

Macromolecule #42: Ribosome biogenesis protein UTP30

• Macromolecule: Name: Ribosome biogenesis protein UTP30 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 28.576559 KDa

Sequence

String:

GLAEKALKAL ILQCEENPSL KNDKDIHIII NTGKKMGINR DNIPRIIPLT KYKLFKPRDL NILLITKDPS ALYRETLTKD EHTSELFKE IISVKNLRRR FKGSKLTQLY KDFDLVVADY RVHHLLPEVL GSRFYHGSKK LPYMIRMSKE VKLKRQQMVE K CDPIYVRA QLRSICKNTS YIPNNDNCLS VRVGYIQKHS IPEILQNIQD TINFLTDKSK RPQGGVIKGG IISIFVKTSN ST SLPIYQF

UniProtKB: Ribosome biogenesis protein UTP30

Macromolecule #43: Pre-rRNA-processing protein PNO1

• Macromolecule: Name: Pre-rRNA-processing protein PNO1 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 20.189639 KDa

Sequence

String:

SRKIMVPPHR MTPLRNSWTK IYPPLVEHLK LQVRMNLKTK SVELRTNPKF TTDPGALQKG ADFIKAFTLG FDLDDSIALL RLDDLYIET FEVKDVKTLT GDHLSRAIGR IAGKDGKTKF AIENATRTRI VLADSKIHIL GGFTHIRMAR ESVVSLILGS P PGKVYGNL RTVASRLKER Y

UniProtKB: Pre-rRNA-processing protein PNO1

Macromolecule #44: rRNA-processing protein FCF2

• Macromolecule: Name: rRNA-processing protein FCF2 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 20.139271 KDa

Sequence

String:

SKFQEIETNL KKLPKLETGF DALANKKKKK NVLPSVETED KRKPNKSDKN DNDWFTLPKP DDNMRREVQR DLLLIKHRAA LDPKRHYKK QRWEVPERFA IGTIIEDKSE FYSSRMNRKE RKSTILETLM GDEASNKYFK RKYNEIQEKS TSGRKAHYKK M KEMRKKR

UniProtKB: rRNA-processing protein FCF2

Macromolecule #45: 40S ribosomal protein S23-A

• Macromolecule: Name: 40S ribosomal protein S23-A / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 11.280271 KDa

Sequence

String:

SPFGGSSHAK GIVLEKLGIE SKQPNSAIRK CVRVQLIKNG KKVTAFVPND GCLNFVDEND EVLLAGFGRK GKAKGDIPGV RFKVVKVSG VSLLALWKEK KEKPR

UniProtKB: Small ribosomal subunit protein uS12A

Macromolecule #46: U3 small nucleolar RNA-associated protein 14

• Macromolecule: Name: U3 small nucleolar RNA-associated protein 14 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 21.558754 KDa

Sequence

String:

QRIQQRHDRK AAYEISRQEV SKWNDIVQQN RRADHLIFPL NKPTEHNHAS AFTRTQDVPQ TELQEKVDQV LQESN(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)NVIINEKVN KKNLKYQSSA VPFPFENREQ YERSLRMPIG QEWTSRASHQ ELIKPRIMTK PGQVID PLK AP

Macromolecule #47: Nucleolar complex protein 14

• Macromolecule: Name: Nucleolar complex protein 14 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 89.734891 KDa

Sequence

String:

MAGSQLKNLK AALKARGLTG QTNVKSKNKK NSKRQAKEYD REEKKKAIAE IREEFNPFEI KAARNKRRDG LPSKTADRIA VGKPGISKQ IGEEQRKRAF EARKMMKNKR GGVIDKRFGE RDKLLTEEEK MLERFTRERQ SQSKRNANLF NLEDDEDDGD M FGDGLTHL GQSLSLEDEL ANDEEDFLAS KRFNEDDAEL QQPQRKKTKA EVMKEVIAKS KFYKQERQKA QGIMEDQIDN LD DNFEDVM SELMMTQPKK NPMEPKTDLD KEYDIKVKE(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) KEKLGKFTAV LLRHIIFLSN QNYLKNVQSF KRTQNALISI LKSLSEKYNR ELSEECRDY INEMQARYKK NHFDALSNGD LVFFSIIGIL FSTSDQYHLV ITPALILMSQ FLEQIKFNSL KRIAFGAVLV R IVSQYQRI SKRYIPEVVY FFQKILLTFI VEKENQEKPL DFENIRLDSY ELGLPLDVDF TKKRSTIIPL HTLSTMDTEA HP VDQCVSV LLNVMESLDA TISTVWKSLP AFNEIILPIQ QLLSAYTSKY SDFEKPRNIL NKVEKLTKFT EHIPLALQNH KPV SIPTHA PKYEENFNPD KKSYDPDRTR SEINKMKAQL KKERKFTMKE IRKDAKFEAR QRIEEKNKES SDYHAKMAHI VNTI NTEEG AEKNKYERER KLR

Macromolecule #48: U3 small nucleolar RNA-associated protein 11

• Macromolecule: Name: U3 small nucleolar RNA-associated protein 11 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 29.604078 KDa

Sequence

String:

KLVHDVQKKQ HRERSQLTSR SRYGFLEKHK DYVKRAQDFH RKQSTLKVLR EKAKERNPDE YYHAMHSRKT DAKGLLISSR HGDEEDESL SMDQVKLLKT QDSNYVRTLR QIELKKLEKG AKQLMFKSSG NHTIFVDSRE KMNEFTPEKF FNTTSEMVNR S ENRLTKDQ LAQDISNNRN ASSIMPKESL DKKKLKKFKQ VKQHLQRETQ LKQVQQRMDA QRELLKKGSK KKIVDSSGKI SF KWKKQRK R

UniProtKB: U3 small nucleolar RNA-associated protein 11

Macromolecule #49: Essential nuclear protein 1

• Macromolecule: Name: Essential nuclear protein 1 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 29.998658 KDa

Sequence

String:

ALPEKVIKAY TTVGSILKTW THGKLPKLFK VIPSLRNWQD VIYVTNPEEW SPHVVYEATK LFVSNLTAKE SQKFINLILL ERFRDNIET SEDHSLNYHI YRAVKKSLYK PSAFFKGFLF PLVETGCNVR EATIAGSVLA KVSVPALHSS AALSYLLRLP F SPPTTVFI KILLDKKYAL PYQTVDDCVY YFMRFRILDD GSNGEDATRV LPVIWHKAFL TFAQRYKNDI TQDQRDFLLE TV RQRGHKD IGPEIRRELL AGAS

UniProtKB: Essential nuclear protein 1

Macromolecule #50: rRNA biogenesis protein RRP5

• Macromolecule: Name: rRNA biogenesis protein RRP5 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 30.781115 KDa

Sequence

String:

APESVADFER LLIGNPNSSV VWMNYMAFQL QLSEIEKARE LAERALKTIN FREEAEKLNI WIAMLNLENT FGTEETLEEV FSRACQYMD SYTIHTKLLG IYEISEKFDK AAELFKATAK KFGGEKVSIW VSWGDFLISH NEEQEARTIL GNALKALPKR N HIEVVRKF AQLEFAKGDP ERGRSLFEGL VADAPKRIDL WNVYVDQEVK AKDKKKVEDL FERIITKKIT RKQAKFFFNK WL QFEESEG DEKTIEYVKA KATEYVAS

UniProtKB: rRNA biogenesis protein RRP5

Macromolecule #51: U3 small nucleolar RNA-associated protein 22

• Macromolecule: Name: U3 small nucleolar RNA-associated protein 22 / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 130.146141 KDa

Sequence

String:

LFKSNIFKLQ IDELLEQVKL KQKHVLKVEK FLHKLYDILQ EIPDWEEKSL AEVDSFFKNK IVSVPFVDPK PIPQNTNYKF NYKKPDISL IGSFALKAGI YQPNGSSIDT LLTMPKELFE KKDFLNFRCL HKRSVYLAYL THHLLILLKK DKLDSFLQLE Y SYFDNDPL LPILRISCSK PTGDSLSDYN FYKTRFSINL LIGFPYKVFE PKKLLPNRNC IRIAQESKEQ SLPATPLYNF SV LSSSTHE NYLKYLYKTK KQTESFVEAT VLGRLWLQQR GFSSNMSHSG SLGGFGTFEF TILMAALLNG GGINSNKILL HGF SSYQLF KGVIKYLATM DLCHDGHLQF HSNPENSSSS PASKYIDEGF QTPTLFDKST KVNILTKMTV SSYQILKEYA GETL RMLNN VVQDQFSNIF LTNISRFDNL KYDLCYDVQL PLGKYNNLET SLAATFGSME RVKFITLENF LAHKITNVAR YALGD RIKY IQIEMVGQKS DFPITKRKVY SNTGGNHFNF DFVRVKLIVN PSECDKLVTK GPAHSETMST EAAVFKNFWG IKSSLR RFK DGSITHCCVW STSSSEPIIS SIVNFALQKH VSKKAQISNE TIKKFHNFLP LPNLPSSAKT SVLNLSSFFN LKKSFDD LY KIIFQMKLPL SVKSILPVGS AFRYTSLCQP VPFAYSDPDF FQDVILEFET SPKWPDEITS LEKAKTAFLL KIQEELSA N SSTYRSFFSR DESIPYNLEI VTLNILTPEG YGFKFRVLTE RDEILYLRAI ANARNELKPE LEATFLKFTA KYLASVRHT RTLENISHSY QFYSPVVRLF KRWLDTHLLL GHITDELAEL IAIKPFVDPA PYFIPGSLEN GFLKVLKFIS QWNWKDDPLI LDLVKPEDD IRDTFETSIG AGSELDSKTM KKLSERLTLA QYKGIQMNFT NLRNSDPNGT HLQFFVASKN DPSGILYSSG I PLPIATRL TALAKVAVNL LQTHGLNQQT INLLFTPGLK DYDFVVDLRT PIGLKSSCGI LSATEFKNIT NDQAPSNFPE NL NDLSEKM DPTYQLVKYL NLKYKNSLIL SSRKYIGVNG GEKGDKNVIT GLIKPLFKGA HKFRVNLDCN VKPVDDENVI LNK EAIFHE IAAFGNDMVI NFETD

UniProtKB: U3 small nucleolar RNA-associated protein 22

Macromolecule #52: Ribosomal RNA-processing protein 7

• Macromolecule: Name: Ribosomal RNA-processing protein 7 / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 21.53241 KDa

Sequence

String:

IEDISAMKNG FIVVPFKLPD HKALPKSQEA SLHFMFAKRH QSSNSNESDC LFLVNLPLLS NIEHMKKFVG QLCGKYDTVS HVEELLYND EFGLHEVDLS ALTSDLMSST DVNEKRYTPR NTALLKFVDA ASINNCWNAL KKYSNLHAKH PNELFEWTYT T PSFTTFVN FYKPLDIDYL KEDIHTHMA

UniProtKB: Ribosomal RNA-processing protein 7

Macromolecule #54: Nucleolar complex protein 4

• Macromolecule: Name: Nucleolar complex protein 4 / type: protein_or_peptide / ID: 54 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 59.433219 KDa

Sequence

String:

MVLLISEIKD IAKRLTAAGD RKQYNSIIKL INELVIPENV TQLEEDETEK NLRFLVMSLF QIFRKLFSRG DLTLPSSKKS TLEKEQFVN WCRKVYEAFK TKLLAIISDI PFETSLGLDS LDVYLQLAEL ESTHFASEKG APFFPNKTFR KLIIALWSSN M GEIEDVKS SGASENLIIV EFTEKYYTKF ADIQYYFQSE FNQLLEDPAY QDLLLKNVGK WLALVNHDKH CSSVDADLEI FV PNPPQAI ENESKFKSNF EKNWLSLLNG QLSLQQYKSI LLILHKRIIP HFHTPTKLMD FLTDSYNLQS SNKNAGVVPI LAL NGLFEL MKRFNLEYPN FYMKLYQIIN PDLMHVKYRA RFFRLMDVFL SSTHLSAHLV ASFIKKLARL TLESPPSAIV TVIP FIYNL IRKHPNCMIM LHNPAFISNP FQTPDQVANL KTLKENYVDP FDVHESDPEL THALDSSLWE LASLMEHYHP NVATL AKIF AQPFKKLSYN MEDFLDWNYD SLLNAESSR

UniProtKB: Nucleolar complex protein 4

Macromolecule #55: U3 small nucleolar RNA-associated protein 8

• Macromolecule: Name: U3 small nucleolar RNA-associated protein 8 / type: protein_or_peptide / ID: 55 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 77.467344 KDa

Sequence

String:

DYVQVADGTF NESTNNITLG ISGSSISQYI INPTPKLTFD YPIPSTNIIT ACNAEKGQAN IDGNIEASTD DEANNEKTIN TQKKRNVEI WAFGLMVNKG NYTLNVITKA LEDTTDTSND HLSESDIDNK AYTGSDEFLS QYKIKAKAKV MSIKIDTKNS L VIAILQNG LIEIFDFKLT LLHSFDISYD NLKYAKWFTE NGTEYVFVLC PLQDDKVCYK LLELTDCGSG ESSPIKELSS TI IEGFSFE NSKLCYQFGK LYKLNQGKIY IYSLPHCQLQ QVIEFPMVDK LSPGDDLISF QPVSVNRVLL TVNNVIYLLD LLH CSTLSQ RELTHVKTFQ LLKSAVINSE KSHNSKTIAI GISTKNGPNP TSSLEIINID VGTNTLKDSL GKSFQVGNND SSVI LKPLF DDKDINDKRV KCNDVSGDSS VPVLHCNEVI EKLSALQDND ITSFDDIFFK ELKIKEEHYT EKDRYISDPG FLNKV LDLI FGKFSGNDYP KTLTFLLTHP LFPLSRTRNL LSLLRDQPRL FKQAIVTCPN LPLNELLEEL FSIRNRELLL DISFRI LQD FTRDSIKQEM KKLSKLDVQN FIEFITSGGE DSSPECFNPS QSTQLFQLLS LVLDSIGLFS LEGALLENLT LYIDKQV EI AERNTELWNL IDTKGFQHGF ASSTFDNGTS QKRALPTYTM EYLDI

UniProtKB: U3 small nucleolar RNA-associated protein 8

Macromolecule #56: Bud site selection protein 21

• Macromolecule: Name: Bud site selection protein 21 / type: protein_or_peptide / ID: 56 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 7.070449 KDa

Sequence

String:

IKTKKRKTLK NLRKDSVKRG KFRVQLLSTT QDSKTLPPKK ESSIIRSKDR WLNRKALNKG

UniProtKB: U3 small nucleolar RNA-associated protein 16

Macromolecule #57: U3 small nucleolar RNA-associated protein 13

• Macromolecule: Name: U3 small nucleolar RNA-associated protein 13 / type: protein_or_peptide / ID: 57 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 90.455766 KDa

Sequence

String:

YKGISLNPIY AGSSAVATVS ENGKILATPV LDEINIIDLT PGSRKILHKI SNEDEQEITA LKLTPDGQYL TYVSQAQLLK IFHLKTGKV VRSMKISSPS YILDADSTST LLAVGGTDGS IIVVDIENGY ITHSFKGHGG TISSLKFYGQ LNSKIWLLAS G DTNGMVKV WDLVKRKCLH TLQEHTSAVR GLDIIEVPDN DEPSLNLLSG GRDDIINLWD FNMKKKCKLL KTLPVNQQVE SC GFLKDGD GKRIIYTAGG DAIFQLIDSE SGSVLKRTNK PIEELFIIGV LPILSNSQMF LVLSDQTLQL INVEEDLKND EDT IQVTSS IAGNHGIIAD MRYVGPELNK LALATNSPSL RIIPVPDLSG PEASLPLDVE IYEGHEDLLN SLDATEDGLW IATA SKDNT AIVWRYNENS CKFDIYAKYI GHSAAVTAVG LPNIVSKGYP EFLLTASNDL TIKKWIIPKP TASMDVQIIK VSEYT RHAH EKDINALSVS PNDSIFATAS YDKTCKIWNL ENGELEATLA NHKRGLWDVS FCQYDKLLAT SSGDKTVKIW SLDTFS VMK TLEGHTNAVQ RCSFINKQKQ LISCGADGLI KIWDCSSGEC LKTLDGHNNR LWALSTMNDG DMIVSADADG VFQFWKD CT EQEIEEEQEK AKLQVEQEQS LQNYMSKGDW TNAFLLAMTL DHPMRLFNVL KRALGESRSR QDTEEGKIEV IFNEELDQ A ISILNDEQLI LLMKRCRDWN TNAKTHTIAQ RTIRCILMHH NIAKLSEIPG MVKIVDAIIP YTQRHFTRVD NLVEQSYIL DYALVEMDKL F

UniProtKB: U3 small nucleolar RNA-associated protein 13

Macromolecule #58: Protein FAF1

• Macromolecule: Name: Protein FAF1 / type: protein_or_peptide / ID: 58 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 27.095045 KDa

Sequence

String:

KTQPKVIRFN GPSDVYVPPS KKTQKLLRSG KTLTQINKKL ESTEAKEEKE DETLEAENLQ NDLELQQFLR ESHLLSAFNN GGSGSTNSG VSLTLQSMGG GNDDGIVYQD DQVIGKARSR TLEMRLNRLS RVNGHQDKIN KLEKVPMHIR RGMIDKHVKR I KKYEQEAA EGGIVLSKVK KGQFRKIEST YKKDIERRIG GSIKARDKEK ATKRERGLKI SSVGRSTRNG LIVSKRDIAR IS

UniProtKB: Protein FAF1

Macromolecule #59: Nucleolar protein 58

• Macromolecule: Name: Nucleolar protein 58 / type: protein_or_peptide / ID: 59 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 47.80873 KDa

Sequence

String:

AYVLTETSAG YALLKASDKK IYKSSSLIQD LDSSDKVLKE FKIAAFSKFN SAANALEEAN SIIEGKVSSQ LEKLLEEIKK DKKSTLIVS ETKLANAINK LGLNFNVVSD AVTLDIYRAI KEYLPELLPG MSDNDLSKMS LGLAHSIGRH KLKFSADKVD V MIIQAIAL LDDLDKELNT YAMRCKEWYG WHFPELAKIV TDSVAYARII LTMGIRSKAS ETDLSEILPE EIEERVKTAA EV SMGTEIT QTDLDNINAL AEQIVEFAAY REQLSNYLSA RMKAIAPNLT QLVGELVGAR LIAHSGSLIS LAKSPASTIQ ILG AEKALF RALKTKHDTP KYGLLYHASL VGQATGKNKG KIARVLAAKA AVSLRYDALA EDRDDSGDIG LESRAKVENR LSQL EGRDL RTTPKVVREA KKVEMTEARA YNADADTAKA A

UniProtKB: Nucleolar protein 58

Macromolecule #60: Regulator of rDNA transcription protein 14

• Macromolecule: Name: Regulator of rDNA transcription protein 14 / type: protein_or_peptide / ID: 60 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 10.916149 KDa

Sequence

String:

RKYLNKLIKR NSQNLRSWDL EEEVRDELED IQQSILKDTV STANTDRSKR RRFKRKQFKE DIKESDFVKD HRYPGLTPGL APVGLSDEE DSS

UniProtKB: Regulator of rDNA transcription protein 14

Macromolecule #61: U3 small nucleolar RNA-associated protein 20

• Macromolecule: Name: U3 small nucleolar RNA-associated protein 20 / type: protein_or_peptide / ID: 61 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 278.852688 KDa

Sequence

String:

QRQTTKSSKR YRYSSFKARI DDLKIEPARN LEKRVHDYVE SSHFLASFDQ WKEINLSAKF TEFAAEIEHD VQTLPQILYH DKKIFNSLV SFINFHDEFS LQPLLDLLAQ FCHDLGPDFL KFYEEAIKTL INLLDAAIEF ESSNVFEWGF NCLAYIFKYL S KFLVKKLV LTCDLLIPLL SHSKEYLSRF SAEALSFLVR KCPVSNLREF VRSVFEKLEG DDEQTNLYEG LLILFTESMT ST QETLHSK AKAIMSVLLH EALTKSSPER SVSLLSDIWM NISKYASIES LLPVYEVMYQ DFNDSLDATN IDRILKVLTT IVF SESGRK IPDWNKITIL IERIMSQSEN CASLSQDKVA FLFALFIRNS DVKTLTLFHQ KLFNYALTNI SDCFLEFFQF ALRL SYERV FSFNGLKFLQ LFLKKNWQSQ GKKIALFFLE VDDKPELQKV REVNFPEEFI LSIRDFFVTA EINDSNDLFE IYWRA IIFK YSKLQNTEII IPLLERIFST FASPDNFTKD MVGTLLKIYR KEDDASGNNL LKTILDNYEN YKESLNFLRG WNKLVS NLH PSESLKGLMS HYPSLLLSLT DNFMLPDGKI RYETLELMKT LMILQGMQVP DLLSSCMVIE EIPLTLQNAR DLTIRIK NV GAEFGKTKTD KLVSSFFLKY LFGLLTVRFS PVWTGVFDTL PNVYTKDEAL VWKLVLSFIK LPDENQNLDY YQPLLEDG A NKVLWDSSVV RLRDTIDTFS HIWSKYSTQN TSIISTTIER RGNTTYPILI RNQALKVMLS IPQVAENHFV DIAPFVYND FKTYKDEEDM ENERVITGSW TEVDRNVFLK TLSKFKNIKN VYSATELHDH LMVLLGSRNT DVQKLALDAL LAYKNPTLNK YRDNLKNLL DDTLFKDEIT TFLTENGSQS IKAEDEKVVM PYVLRIFFGR AQVPPTSGQK RSRKIAVISV LPNFKKPYIN D FLSLASER LDYNYFFGNS HQINSSKATL KTIRRMTGFV NIVNSTLSVL RTNFPLHTNS VLQPLIYSIA MAYYVLDTES TE EVHLRKM ASNLRQQGLK CLSSVFEFVG NTFDWSTSME DIYAVVVKPR ISHFSDENLQ QPSSLLRLFL YWAHNPSLYQ FLY YDEFAT ATALMDTISN QHVKEAVIGP IIEAADSIIR NPVNDDHYVD LVTLICTSCL KILPSLYVKL SDSNSISTFL NLLV SITEM GFIQDDHVRS RLISSLISIL KGKLKKLQEN DTQKILKILK LIVFNYNCSW SDIEELYTTI SSLFKTFDER NLRVS LTEL FIELGRKVPE LESISKLVAD LNSYSSSRMH EYDFPRILST FKGLIEDGYK SYSELEWLPL LFTFLHFINN KEELAL RTN ASHAIMKFID FINEKPNLNE ASKSISMLKD ILLPNIRIGL RDSLEEVQSE YVSVLSYMVK NTKYFTDFED MAILLYN GD EEADFFTNVN HIQLHRRQRA IKRLGEHAHQ LKDNSISHYL IPMIEHYVFS DDERYRNIGN ETQIAIGGLA QHMSWNQY K ALLRRYISML KTKPNQMKQA VQLIVQLSVP LRETLRIVRD GAESKLTLSK FPSNLDEPSN FIKQELYPTL SKILGTRDD ETIIERMPIA EALVNIVLGL TNDDITNFLP SILTNICQVL RSKSEELRDA VRVTLGKISI ILGAEYLVFV IKELMATLKR GSQIHVLSY TVHYILKSMH GVLKHSDLDT SSSMIVKIIM ENIFGFAGEE KDSENYHTKV KEIKSNKSYD AGEILASNIS L TEFGTLLS PVKALLMVRI NLRNQNKLSE LLRRYLLGLN HNSDSESESI LKFCHQLFQE SEMSNSPQIP KKKVKDQVDE KE DFFLVNL ESKSYTINSN SLLLNSTLQK FALDLLRNVI TRHRSFLTVS HLEGFIPFLR DSLLSENEGV VISTLRILIT LIR LDFSDE SSEIFKNCAR KVLNIIKVSP STSSELCQMG LKFLSAFIRH TDSTLKDTAL SYVLGRVLPD LNEPSRQGLA FNFL KALVS KHIMLPELYD IADTTREIMV TNHSKEIRDV SRSVYYQFLM EYDQSKGRLE KQFKFMVDNL QYPTESGRQS VMELI NLII TKANPALLSK LSSSFFLALV NVSFNDDAPR CREMASVLIS TMLPKLENKD LEIVEKYIAA WLKQVDNASF LNLGLR TYK VYLKSIGFEH TIELDELAIK RIRYILSDTS VGSEHQWDLV YSALNTFSSY MEATESVYKH GFKDIWDGII TCLLYPH SW VRQSAANLVH QLIANKDKLE ISLTNLEIQT IATRILHQLG APSIPENLAN VSIKTLVNIS ILWKEQRTPF IMDVSKQT G EDLKYTTAID YMVTRIGGII RSDEHRMDSF MSKKACIQLL ALLVQVLDED EVIAEGEKIL LPLYGYLETY YSRAVDEEQ EELRTLSNEC LKILEDKL

UniProtKB: U3 small nucleolar RNA-associated protein 20

Macromolecule #62: RNA cytidine acetyltransferase

• Macromolecule: Name: RNA cytidine acetyltransferase / type: protein_or_peptide / ID: 62 / Number of copies: 2 / Enantiomer: LEVO; EC number: Transferases; Acyltransferases; Transferring groups other than aminoacyl groups
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 104.469625 KDa

Sequence

String:

AKKAIDSRIP SLIRNGVQTK QRSIFVIVGD RARNQLPNLH YLMMSADLKM NKSVLWAYKK KLLGFTSHRK KRENKIKKEI KRGTREVNE MDPFESFISN QNIRYVYYKE SEKILGNTYG MCILQDFEAL TPNLLARTIE TVEGGGIVVI LLKSMSSLKQ L YTMTMDVH ARYRTEAHGD VVARFNERFI LSLGSNPNCL VVDDELNVLP LSGAKNVKPL PPKEDDELPP KQLELQELKE SL EDVQPAG SLVSLSKTVN QAHAILSFID AISEKTLNFT VALTAGRGRG KSAALGISIA AAVSHGYSNI FVTSPSPENL KTL FEFIFK GFDALGYQEH IDYDIIQSTN PDFNKAIVRV DIKRDHRQTI QYIVPQDHQV LGQAELVVID EAAAIPLPIV KNLL GPYLV FMASTINGYE GTGRSLSLKL IQQLRNQNNT SGRESTQTAV VSRDNKEKDS HLHSQSRQLR EISLDEPIRY APGDP IEKW LNKLLCLDVT LIKNPRFATR GTPHPSQCNL FVVNRDTLFS YHPVSENFLE KMMALYVSSH YKNSPNDLQL MSDAPA HKL FVLLPPIDPK DGGRIPDPLC VIQIALEGEI SKESVRNSLS RGQRAGGDLI PWLISQQFQD EEFASLSGAR IVRIATN PE YASMGYGSRA IELLRDYFEG KFTDMSEDVR PKDYSIKRVS DKELAKTNLL KDDVKLRDAK TLPPLLLKLS EQPPHYLH Y LGVSYGLTQS LHKFWKNNSF VPVYLRQTAN DLTGEHTCVM LNVLEGRESN WLVEFAKDFR KRFLSLLSYD FHKFTAVQA LSVIESSKKA QDLSDDEKHD NKELTRTHLD DIFSPFDLKR LDSYSNNLLD YHVIGDMIPM LALLYFGDKM GDSVKLSSVQ SAILLAIGL QRKNIDTIAK ELNLPSNQTI AMFAKIMRKM SQYF

UniProtKB: RNA cytidine acetyltransferase

Macromolecule #63: 40S ribosomal protein S6-A

• Macromolecule: Name: 40S ribosomal protein S6-A / type: protein_or_peptide / ID: 63 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 25.123168 KDa

Sequence

String:

MKLNISYPVN GSQKTFEIDD EHRIRVFFDK RIGQEVDGEA VGDEFKGYVF KISGGNDKQG FPMKQGVLLP TRIKLLLTKN VSCYRPRRD GERKRKSVRG AIVGPDLAVL ALVIVKKGEQ ELEGLTDTTV PKRLGPKRAN NIRKFFGLSK EDDVRDFVIR R EVTKGEKT YTKAPKIQRL VTPQRLQRKR HQRALKVRNA QAQREAAAEY AQLLAKRLSE R

UniProtKB: Small ribosomal subunit protein eS6A

Macromolecule #64: 40S ribosomal protein S13

• Macromolecule: Name: 40S ribosomal protein S13 / type: protein_or_peptide / ID: 64 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 14.16459 KDa

Sequence

String:

LSSESVIEQI VKYARKGLTP SQIGVLLRDA HGVTQARVIT GNKIMRILKS NGLAPEIPED LYYLIKKAVS VRKHLERNRK DKDAKFRLI LIESRIHRLA RYYRTVAVLP PNWKYESATA SALVN

UniProtKB: Small ribosomal subunit protein uS15

Macromolecule #65: Protein BFR2

• Macromolecule: Name: Protein BFR2 / type: protein_or_peptide / ID: 65 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 61.285039 KDa

Sequence

String:

MEKSLADQIS DIAIKPVNKD FDIEDEENAS LFQHNEKNGE SDLSDYGNSN TEETKKAHYL EVEKSKLRAE KGLELNDPKY TGVKGSRQA LYEEVSENED EEEEEEEEEE KEEDALSFRT DSEDEEVEID EEESDADGGE TEEAQQKRHA LSKLIQQETK Q AINKLSQS VQRDASKGYS ILQQTKLFDN IIDLRIKLQK AVIAANKLPL TTESWEEAKM DDSEETKRLL KENEKLFNNL FN RLINFRI KFQLGDHITQ NEEVAKHKLS KKRSLKELYQ ETNSLDSELK EYRTAVLNKW STKVSSASGN AALSSNKFKA INL PADVQV ENQLSDMSRL MKRTKLNRRN ITPLYFQKDC ANGRLPELIS PVVKDSVDDN ENSDDGLDIP KNYDPRRKDN NAID ITENP YVFDDEDFYR VLLNDLIDKK ISNAHNSESA AITITSTNAR SNNKLKKNID TKASKGRKLN YSVQDPIANY EAPIT SGYK WSDDQIDEFF AGLLGQRVNF NENEDEEQHA RIENDEELEA VKNDDIQIFG

UniProtKB: Protein BFR2

Macromolecule #66: U3 small nucleolar ribonucleoprotein protein LCP5

• Macromolecule: Name: U3 small nucleolar ribonucleoprotein protein LCP5 / type: protein_or_peptide / ID: 66 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 40.864391 KDa

Sequence

String:

MSELNALLKD INGSLTATSE SLERLSGIYS NSATDEIPES NQLHEHLFYD AKKPAEKVSL LSLKNGSMLG YINSLLMLIG NRLDDECKD PSAMDARERS IQHRVVLERG VKPLEKKLAY QLDKLTRAYV KMEKEYKDAE KRALEKSTLV NHSGNDDSED D ESSEDEIA YRPNTSGIIN TNKKSSAYRV EETAKQENGE ENDDNETGVY KPPKITAVLP PQQTHFEDRF DAREHKDRSN KS RMQAMEE YIRESSDQPD WSASIGADIV NHGRGGIKSL RDTEKERRVT SFEEDNFTRL NITNKAEKRK QKQRERNARM NVI GGEDFG IFSSKRKLED STSRRGAKKT RSAWDRAQRR L

UniProtKB: U3 small nucleolar ribonucleoprotein protein LCP5

Macromolecule #4: 5' ETS

• Macromolecule: Name: 5' ETS / type: rna / ID: 4 / Number of copies: 1
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 225.543094 KDa

Sequence

String:

AUGCGAAAGC AGUUGAAGAC AAGUUCGAAA AGAGUUUGGA AACGAAUUCG AGUAGGCUUG UCGUUCGUUA UGUUUUUGUA AAUGGCCUC GUCAAACGGU GGAGAGAGUC GCUAGGUGAU CGUCAGAUCU GCCUAGUCUC UAUACAGCGU GUUUAAUUGA C AUGGGUUG AUGCGUAUUG AGAGAUACAA UUUGGGAAGA AAUUCCCAGA GUGUGUUUCU UUUGCGUUUA ACCUGAACAG UC UCAUCGU GGGCAUCUUG CGAUUCCAUU GGUGAGCAGC GAAGGAUUUG GUGGAUUACU AGCUAAUAGC AAUCUAUUUC AAA GAAUUC AAACUUGGGG GAAUGCCUUG UUGAAUAGCC GGUCGCAAGA CUGUGAUUCU UCAAGUGUAA CCUCCUCUCA AAUC AGCGA UAUCAAACGU ACCAUUCCGU GAAACACCGG GGUAUCUGUU UGGUGGAACC UGAUUAGAGG AAACUCAAAG AGUGC UAUG GUAUGGUGAC GGAGUGCGCU GGUCAAGAGU GUAAAAGCUU UUUGAACAGA GAGCAUUUCC GGCAGCAGAG AGACCU GAA AAAGCAAUUU UUCUGGAAUU UCAGCUGUUU CCAAACUCAA UAAGUAUCUU CUAGCAAGAG GGAAUAGGUG GGAAAAA AA AAAAGAGAUU UCGGUUUCUU UCUUUUUUAC UGCUUGUUGC UUCUUCUUUU AAGAUAGU

GENBANK: GENBANK: NR_132207.1

Macromolecule #5: U3 snoRNA

• Macromolecule: Name: U3 snoRNA / type: rna / ID: 5 / Number of copies: 1
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 106.503258 KDa

Sequence

String:

GUCGACGUAC UUCAUAGGAU CAUUUCUAUA GGAAUCGUCA CUCUUUGACU CUUCAAAAGA GCCACUGAAU CCAACUUGGU UGAUGAGUC CCAUAACCUU UGUACCCCAG AGUGAGAAAC CGAAAUUGAA UCUAAAUUAG CUUGGUCCGC AAUCCUUAGC G GUUCGGCC AUCUAUAAUU UUGAAUAAAA AUUUUGCUUU GCCGUUGCAU UUGUAGUUUU UUCCUUUGGA AGUAAUUACA AU AUUUUAU GGCGCGAUGA UCUUGACCCA UCCUAUGUAC UUCUUUUUUG AAGGGAUAGG GCUCUAUGGG UGGGUACAAA UGG CAGUCU GACAAGU

GENBANK: GENBANK: LN809883.1

Macromolecule #53: 18S pre-rRNA

• Macromolecule: Name: 18S pre-rRNA / type: rna / ID: 53 / Number of copies: 1
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 582.052438 KDa

Sequence

String:

AGAUAGUUAU CUGGUUGAUC CUGCCAGUAG UCAUAUGCUU GUCUCAAAGA UUAAGCCAUG CAUGUCUAAG UAUAAGCAAU UUAUACAGU GAAACUGCGA AUGGCUCAUU AAAUCAGUUA UCGUUUAUUU GAUAGUUCCU UUACUACAUG GUAUAACUGU G GUAAUUCU AGAGCUAAUA CAUGCUUAAA AUCUCGACCC UUUGGAAGAG AUGUAUUUAU UAGAUAAAAA AUCAAUGUCU UC GGACUCU UUGAUGAUUC AUAAUAACUU UUCGAAUCGC AUGGCCUUGU GCUGGCGAUG GUUCAUUCAA AUUUCUGCCC UAU CAACUU UCGAUGGUAG GAUAGUGGCC UACCAUGGUU UCAACGGGUA ACGGGGAAUA AGGGUUCGAU UCCGGAGAGG GAGC CUGAG AAACGGCUAC CACAUCCAAG GAAGGCAGCA GGCGCGCAAA UUACCCAAUC CUAAUUCAGG GAGGUAGUGA CAAUA AAUA ACGAUACAGG GCCCAUUCGG GUCUUGUAAU UGGAAUGAGU ACAAUGUAAA UACCUUAACG AGGAACAAUU GGAGGG CAA GUCUGGUGCC AGCAGCCGCG GUAAUUCCAG CUCCAAUAGC GUAUAUUAAA GUUGUUGCAG UUAAAAAGCU CGUAGUU GA ACUUUGGGCC CGGUUGGCCG GUCCGAUUUU UUCGUGUACU GGAUUUCCAA CGGGGCCUUU CCUUCUGGCU AACCUUGA G UCCUUGUGGC UCUUGGCGAA CCAGGACUUU UACUUUGAAA AAAUUAGAGU GUUCAAAGCA GGCGUAUUGC UCGAAUAUA UUAGCAUGGA AUAAUAGAAU AGGACGUUUG GUUCUAUUUU GUUGGUUUCU AGGACCAUCG UAAUGAUUAA UAGGGACGGU CGGGGGCAU CAGUAUUCAA UUGUCAGAGG UGAAAUUCUU GGAUUUAUUG AAGACUAACU ACUGCGAAAG CAUUUGCCAA G GACGUUUU CAUUAAUCAA GAACGAAAGU UAGGGGAUCG AAGAUGAUCA GAUACCGUCG UAGUCUUAAC CAUAAACUAU GC CGACUAG GGAUCGGGUG GUGUUUUUUU AAUGACCCAC UCGGCACCUU ACGAGAAAUC AAAGUCUUUG GGUUCUGGGG GGA GUAUGG UCGCAAGGCU GAAACUUAAA GGAAUUGACG GAAGGGCACC ACCAGGAGUG GAGCCUGCGG CUUAAUUUGA CUCA ACACG GGGAAACUCA CCAGGUCCAG ACACAAUAAG GAUUGACAGA UUGAGAGCUC UUUCUUGAUU UUGUGGGUGG UGGUG CAUG GCCGUUCUUA GUUGGUGGAG UGAUUUGUCU GCUUAAUUGC GAUAACGAAC GAGACCUUAA CCUACUAAAU AGUGGU GCU AGCAUUUGCU GGUUAUCCAC UUCUUAGAGG GACUAUCGGU UUCAAGCCGA UGGAAGUUUG AGGCAAUAAC AGGUCUG UG AUGCCCUUAG ACGUUCUGGG CCGCACGCGC GCUACACUGA CGGAGCCAGC GAGUCUAACC UUGGCCGAGA GGUCUUGG U AAUCUUGUGA AACUCCGUCG UGCUGGGGAU AGAGCAUUGU AAUUAUUGCU CUUCAACGAG GAAUUCCUAG UAAGCGCAA GUCAUCAGCU UGCGUUGAUU ACGUCCCUGC CCUUUGUACA CACCGCCCGU CGCUAGUACC GAUUGAAUGG CUUAGUGAGG CCUCAGGAU CUGCUUAGAG AAGGGGGCAA CUCCAUCUCA GAGCGGAGAA UUUGGACAAA CUUGGUCAUU UAGAGGAACU A AAAGUCGU AACAAGGUUU CCGUAGGUGA ACCUGCGGAA GGAUCAUUA

GENBANK: GENBANK: CP011558.1

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Buffer: pH: 7.7
• Vitrification: Cryogen name: ETHANE

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Image recording: Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 1.074 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 1.3 µm
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• Startup model: Type of model: NONE
• Final reconstruction: Resolution.type: BY AUTHOR / Resolution: 3.99 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 199534
• Initial angle assignment: Type: NOT APPLICABLE
• Final angle assignment: Type: MAXIMUM LIKELIHOOD